aeruginosa Escherichia Abu-Hanifah Nichols Pseudomonas aeruginosa

Transcription

1 . Pseudomonas Klebsiella Proteus mirabilis 9. Staphylococcus.9.6 pneumoniae Pseudomonas Staphylococcus, Oguntibeju Nwobu Escherichia Klebsiella coli Proteus spp. pneumoniae 199 Fauci Serratia marcescens Citrobacter youngae Acinetobacter baumanni AbuHanifah Nichols Dhidah Nichols Lechmann Graig 1

2 National Committee of Clinical Laboratory Standars(NCCLS)00 Harley Prescott Harbone Harbone Baron Eosin Collee methylene Finegold blue Nag Inhibition Zone Harsh Saxena Kirby Hindeler Bauer 0.1 Proteus mirabilis Pseudomonas Klebsiella pneumoniae Staphylococcus ;Collee ; Betty ; Brooks 1

3 Motility Indol Simmon s Citrate Vogesproskauer Methylred Urease Eosin Methylene blue Oxidase V V=Variable,=Positive,=Negative V V Pseudomonas Proteus mirabilis Klebsiella pneumoniae Coagulase Staphylococcus Jawetz Klebsiella.6 K.pneumoniae E.coli pneumoniae. Pseudomonas Nwobu. Brooks.6. Oguntibeju. Jawetz.9.6 Staphylococcus 9..1 Nichols 001 Landman ; Jarvis 11.9 Proteus mirabilis Proteus spp

4 Pseudomonas Staphylococcus Proteus mirabilis Klebsiella pneumoniae Total Nalidixic AlYaseri David; Trimethoprim acid Gupta Jawetz Pseudomonas Tetracyclin Gentamicin Oguntibeju Nwobu Tertracyclin Gentamicin Daley 1. Staphylococcus Tetracyclin Nalidixic acid 1

5 1 % Tetracyclin% Ciprofloxicin% Gentamicin% Trimethoprim% Nitrofurantoin% Nalidixic acid% Rifampicin% Chloramphenicol % Pseudomonas 1 (91.0) (1.) 6 (6.0) 0 (6.9) 0 (6.9) 0 (6.9) 0 (6.9) 1 (.91) Staphylococcus 0 16 (0) () (0) () 1 (0) Proteus mirabilis () (.) (.) (.) 6 () (1.) (.) (.) 6 (.1) (.) (.) (.1) (1.) Klbsiella pneumoniae (0) 1 (0) (0) (0) (0) () 1 () () () () Taylor Mahasneh Allistatin I &II Chakravarty Pse. Staph. Pr. mirabilis E.coli K. pneumoniae En. cloacae

6 pipercillin/tazobactam.pathology.: 161. *David,L.; Paterson, W.;Gottberg,A.V.; Casellas, J.M.; and et al.(001). Outcome of cephalosporin treatment for serious infections due to apparently susceptible organisms producing extended spectrum B Lactamase.J.Clin.Microb.,9:06 1. Dhidah,L.;Dhidah,M.;Miladi,M.;Macem,N.; Troudi,M.(001).Nosocomial wound infection. Prvalance Study at Sahloul University.TunisMed.6:010. Fauci,A.S.; Braunwald,B.; Isselbacher,K.J.; Wllson,J.D.;and et al (199). Principles of Internal Medicine.1 th ed.mc GramHill Company.New York. Graig,A.and Lechmann,A.(199).Saunders Manual of Clinical Laboratory Science.1 st ed.saunders Company. Gupta,K. ;Scholes,D.and Stamm, W.E. (1999).Increasing prevalence of antimicrobial resistance among uropathogens causing acute uncomplicated cystitis in women. JAMA.,1:6. Harbone,J.B. (19). Phytochemical Methods Aguide to Modern Techniques of Plant Analysis. nd.london.new York, Chapman and Hall. Harley,J.P. and Prescott, L.M. (1996). Laboratory Exercises in Microbiology. rd ed. McGrawHill Company.U.S.A. Harsh, M.L. and Nag, T.N. (19). Antimicrobial principles from in vitro tissue culture of peganum harmala.j.of Nat.Prod.:66. Hindeler,J.(199).Antimicrobial Susceptibility Testing In:Essential Procedures for Clinical Microbiology Press. Washington. U.S.A.,J.G.; Krieg,N.R.; Sneath,P.H.A.; Staley,J.T. and Williams, S T. (199). Bergeys Manual of AbuHanifah, Y. (1990).PostOperative surgical wound infection.med.j. Malaysia.,:99. AlYaseri,A.J.(199).The Importance of Minimum Inhibitory Concentration of Antimicrobial Against Negative Bacilli Isolated Gram Urinary Tract Infection.M.Sc. Thesis Submitted of College of Medicin,University of Baghdad. Baron,E.J. and Finegold,S.M.(1990).Baileey and Scotts Diagnostic Microbiology. th ed. C.V. Mosby,U.S.A. Betty,D.R.;Klein, J.O. and Launary,V. (199). Epidemiology, microbiology and management of cojunctives paediatrician, Suppl,:101. Brooks, G.F.;Butel,J.S. and Morse,S.A. (001). Jawetz, Melnich and Adelbergs Medical Microbiology. nd ed., McGrawHill Copanies,Inc.,New York. Chakravarty, H.L.(199).Plant Wealth of Iraqi Adictionary Economic Plant. Ministry of Agriculture and Agration Reform, BaghdadIraq. Collee,J.G.;Fraser, A.G.; Marmion, B.P. and Simmons, A. (1996). Mackie and McCartney Practical Medical Microbiology.1 th ed. Churchill Livingstone, New York. Daley,D. ; Mulgrare,L.; Munro,R.; Neville, S.;Smith, I. and Dimcch,W. (1996). Anevalution of the invitro activity of 19

7 Bahren. Plytotherapy Res.,10: National Committee for Clinical Laboratory Standards(NCCLS) (00). Performans Standards for Antimicrobial Susceptibility Testing. Twelfth Information Supplement. Nwobu,R.A.U.and Oguntibeju, O.O. (00). Occurrence of Pseudomonas in postoperative wound infection. Pak.J.Med.Sc.,0:1191. Nichols.R.L. (1991). Surgical wound infection.am.j.med., 91(Suppl.B). 6. Saxena,G.;Farmer,S.; Hancock, R.E.W. and Towers, G.H.N. (199). Antimicrobial compounds from Alnus rubra.int.j.of Pharmcognosy, :6. Taylor,R.S.; Edel,F.; Manan;N. and Towers,G.(1996).Antimicrobial activity of southern nepales medicinal plants.j.of Ethnopharmacology,0:910. Determinative Bacteriology.9 th ed. Williams and Wilkins, Baltimore, U.S.A. Jarvis,W.R.(1996).Selected aspects of the socioeconomic impact of nosocomial infection.morbidity.mortilty.cost and Prevention.Infect.Control Hosp.Epidemiol.,1:. Jawetz,E.;Melnick,J.and Adelberg,E.A.(19).Review of Medical Microbiology.1 th ed. Appelton and Longer,Norwalk,U.S.A. Jawetz,E.; Melnick,J.L. and Adelberg,E.A.(001).Medical Microbiology. nd ed.appleton and Lange Cpmpany.U.S.A. Landman,D.(001).Mangement of infection due to resistance Staphylococcus.Am.J.Infection.0:0. Mahasneh,A.M.;Abbas,J.A. and Eloqilah,A.A.(1996).Antimicrobial activity of extracts of herbal plants used in the traditional medicine of The effect of some antibiotics and Allium sativum extract on the aerobic bacteria isolated from post operative surgical wound infection of AlDiwaniya s teaching hospital in AlDiwaniya city H. M. K. AlAbidi Coll.of Education/Univ.of AlQadisiya Abstract Seventy three samples were collected from operative wound of patients in AlDiwaniya s Teaching Hospital in AlDiwaniya city. Results of the isolation and identification of the bacteria were as fllows :Pseudomonas.%, Staphylococcus 9.%, Proteus mirabilis 11.9%, 10.%, Klebsiella pneumoniae.6%, Enterobacter cloacae.9%.as for effect of antibiotics on bacteria,it was found out that was the most powerful antibiotic while most of bacteria resisted the.the effect Allium sativum extract on growth of bacterial isolated, the results showed the prohibiting effect of the extract increases with increase and differs according to the kind of bacteria. It was also found out that the ethanol extract proved more powerful than aquatic extract. 0