Department of Biology, Microbiology and Biotechnology, Faculty of Science, Federal University, Ndufu-Alike, Ikwo, Nigeria

Save this PDF as:
Size: px
Start display at page:

Download "Department of Biology, Microbiology and Biotechnology, Faculty of Science, Federal University, Ndufu-Alike, Ikwo, Nigeria"

Transcription

1 SciFed Journal of Applied Microbiology Research Article Open Access Frequency and Antibiogram of Urinary Isolates of Klebsiella Pneumoniae Isolated from Urine Samples of Apparently Healthy School Children 1 Okonkwo E C, 1 Orji JO, 2 Nworie O, * 1 Ejikeugwu PC Ejikeugwu PC,, 2018, 1:1 *1 Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, P.M.B.053, Ebonyi State, Nigeria 2 Department of Biology, Microbiology and Biotechnology, Faculty of Science, Federal University, Ndufu-Alike, Ikwo, Nigeria Abstract Antimicrobial resistance is a natural biological phenomenon of bacterial adaption to harsh environment, but this development compromises the efficacy of some available antimicrobials due to the emergence and spread of drug resistant bacteria in both the community and hospital environment. In this study, the prevalence and antimicrobial susceptibility profile of Klebsiella pneumoniae isolated from urine samples of apparently healthy school children in a local primary school in Abakaliki, Nigeria was bacteriologically investigated. A total of 25 urine samples were collected from the school children using sterile urine containers. The isolation of Klebsiella pneumoniae was carried out on eosin methylene blue (EMB) and MacConkey (MAC) agar plates. Culture plates showing significant bacterial growth (>10 CFU/ml) were processed further for the identification of Klebsiella pneumoniae using standard microbiology techniques. Antibiogram was determined using the modified Kirby-Bauer disk diffusion technique as per the CLSI criteria on Mueller-Hinton agar plates. The rate of isolation of Klebsiella pneumoniae from the urine samples of the school children used in this study was high (60%; n=15). The result of the antimicrobial susceptibility testing showed that more than 70% of the Klebsiella pneumoniae isolated were highly resistant or intermediately resistant to ampicillin, chloramphenicol, and clarithromycin and augmentin. This was closely followed by resistance to perfloxacin, ciprofloxacin, ceftriaxone, gentamicin and ofloxacin. Nitrofurantoin had no inhibitory activity against the Klebsiella pneumoniaeisolates recovered in this study. The data provided could help to step up surveillance and monitoring measures geared towards the proper detection and reporting of antimicrobial resistance in the non-hospital environment. Keywords Antimicrobial Resistance; Enterobacteriaceae; Antimicrobial Susceptibility Testing; Gram Negative Bacteria; Nigeria Introduction Antimicrobial resistance is the ability of microorganisms, such as bacteria to grow in the presence of an antimicrobial agent that would normally kill it or inhibit its growth. It is one of the biggest current challenges facing the healthcare sector globally especially in the area of infection control and disease containment. Most Gramnegative bacteria including but not limited to Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa are fast becoming resistant to the onslaught of antimicrobial agents because they harbour and transmit drug resistance genes including but not limited to metallo-β-lactamases (MBLs), AmpC enzymes and extended spectrum β-lactamases known as ESBLs [1, 2]. It is now more *Corresponding author: Ejikeugwu PC, Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, P.M.B.053, Ebonyi State, Nigeria. Tel: Received January 04, 2018; Accepted February 13, 2018; Published February 20, 2018 Citation: Ejikeugwu PC (2018) Frequency and Antibiogram of Urinary Isolates of Klebsiella Pneumoniae Isolated from Urine Samples of Apparently Healthy School Children. 1:1. Copyright: 2017 Ejikeugwu PC. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. page 1 of 6

2 Healthy School Children. 1:1. worrisome that drug resistance in bacteria are not restricted to the hospital environment alone but bacterial pathogens including Klebsiella pneumoniae that are multidrug resistant in nature are now found even in the non-hospital environment which is not known for heavy antibiotic usage. Measuring the prevalence and antibiogram of drug resistant organisms coupled with proper use of available drugs is critical to reducing the possible risks associated with infections due to these organisms [3]. Klebsiella is a genus of non-motile, Gram-negative, oxidase-negative, rod-shaped bacteria with a prominent polysaccharidebased capsule; and they are ubiquitous in the environment [4, 5]. The members of the genus Klebsiella are a part of the normal flora of the gastrointestinal tract (GIT) of man and animals. Infections with Klebsiella pneumoniae can lead to a wide range of disease states, notably pneumonia, urinary tract infections, septicemia, meningitis, diarrhea, and soft tissue infections [4, 5, 6]. The majority of human Klebsiella infections are caused by K. pneumoniae, followed by K. oxytoca. Klebsiella species are sometimes regarded as coliforms as is applicable to E. coli and other enteric bacteria [4, 5]. The antimicrobial resistance nature of Klebsiella species is thought to be attributable mainly to the multidrug efflux pumps of the organism, which allows it to actively pump out antimicrobial agents from the cell [7]. The ability of K. pneumoniae to colonize the hospital environment, including carpeting, sinks, flowers, and various surfaces, as well as the skin of patients and hospital staff, has been identified as a major factor in the spread of hospital-acquired infections including drug resistance genes [8]. Klebsiella species is one of the major causes of both hospital-acquired and community-acquired infections, and some strains of this organism are notoriously resistant to some available antimicrobial agents [2, 6, 8]. It is in view of this that this present study presumptively evaluated the prevalence and antibiogram of Klebsiella pneumoniae isolates recovered from the urine samples of healthy school children in the community. Materials and Methods Study Area and Population The study area of this research was a local primary school located in Abakaliki metropolis of Ebonyi State Nigeria. Both male and female school children were recruited for this study. This study was carried out over a two (2) months period from July to August Sample Collection and Processing A total of 25 school children including male and female were used for this study. The school children were apparently healthy children who presented with no clinical signs and symptoms of any disease. The urine samples were obtained by informed consent of the school children used for this study. Urine samples were aseptically collected in sterile urine bottles containing boric acid from a total of 25 school children. All collected samples were labelled and transported to the Microbiology laboratory unit of Ebonyi State University, Abakaliki within one hour of collection for bacteriological analysis. Culture and identification of Klebsiella pneumoniae A loopful of the urine samples were each plated onto freshly prepared MacConkey (MAC) agar and eosin methylene blue (EMB) agar plates, and incubated at 37 o C for h [9]. All culture plates showing significant bacterial growth (>10 CFU/ml) were further processed by sub culturing onto freshly prepared MAC and EMB agar plates for isolation of pure cultures, and for the identification of Klebsiella pneumoniae. The isolated colonies growing on the culture media plate was identified based on their colonial characteristics, microscopy, and biochemical testing. Klebsiella pneumoniae produces mucoid colonies on MacConkey agar and non-metallic green sheen colonies on EMB agar; and they are Gram negative, citrate positive and urease positive [9]. Antibiogram The antimicrobial susceptibility testing was carried out on Mueller-Hinton (MH) agar plates as per the guidelines of the Clinical and Laboratory Standard Institute (CLSI) using the modified Kirby-Bauer disk diffusion technique as was previously described [1, 2]. The following antibiotics were used: ampicillin (30 mcg), nitrofurantoin (100 mcg), ciprofloxacin (10 mcg), gentamicin (10 mcg), ofloxacin (10 mcg), ceftriaxone (30 mcg), chloramphenicol (10 mcg), augmentin (30 mcg), clarithromycin (30 mcg) and perfloxacin (30 mcg). All susceptibility test plates were incubated at 37 o C for h; and inhibition zone diameter(s) were measured, recorded and interpreted using the standard antibiotic breakpoints recommended by the CLSI [10]. The degree of susceptibility of the isolated Klebsiella pneumoniae to the tested antibiotics were interpreted as either sensitive (S) or resistant (R) by measuring their respective inhibition page 2 of 6

3 Healthy School Children. 1:1. zone diameter (IZD) and comparing it to the standard breakpoints of the CLSI. Results Table 1 shows the colonial features, morphology and biochemical reaction of the isolated Klebsiella pneumoniae from the urine samples. Klebsiella pneumoniae produces mucoid colonies on MacConkey agar and nonmetallic green sheen colonies on eosin methylene blue agar (Table 1). A total of 15 (60%) isolates of Klebsiella pneumoniae was bacteriologically recovered from the urine samples. The prevalence of Klebsiella pneumoniae according to the sex of the school children used in this study is shown in (Table 2). It was observed in our study that more of the Klebsiella pneumoniae was isolated from females (69.2%) than males (50.0%). Statistical analysis however shows that there was no significant difference in the rate of isolation of Klebsiella pneumoniae between the male and female school children (p>0.05). Table 3 shows the distribution of the isolated Klebsiella pneumoniae based on the age of the school children used in this study. In relation to their age, Klebsiella pneumoniae was isolated more from school children in the age range of 8-10 years followed by school children in the age range of >12 (Table 3). The isolation of Klebsiella pneumoniae from the school children shows possible contamination from the school environment. Statistically, the occurrence of Klebsiella pneumoniae shows no significant difference as per the age of the school children (p>0.05). Table 1: Isolation of Klebsiella Pneumoniae from Urine Samples Bacteria Sample (n) Isolates n (%) Klebsiella pneumoniae Urine (25) 15 (60) Morphological appearance Mucoid colonies on MAC; nonmetallic green sheen colonies on EMB Gram reaction Gram negative Biochemical reaction Citrate positive Urease positive n-number, %-Percentage, EMB-eosin Methylene Blue, MAC-Mac Conkey Table 2: Prevalence of Klebsiella Pneumoniae in Urine Samples in Relation to sex Sex Number screened of number infected % Male Female Total Table 3: Distribution of Klebsiella Pneumoniae in Urine Sample in Relation to Age Age Number screened % isolation of Klebsiella pneumonia % of number infected > Aug > Total page 3 of 6

4 Healthy School Children. 1:1. The antimicrobial susceptibility pattern of the isolated Klebsiella pneumoniae is shown in Table 4. All the isolated Klebsiella pneumoniae showed varying levels of susceptibility and resistance to the tested antibiotics used in this study. However, the isolated Klebsiella pneumoniae were highly resistant or intermediately resistant to ampicillin, clarithromycin, chloramphenicol, ceftriaxone and ofloxacin. Moreover, this was closely followed by perfloxacin and augmentin (Table 4). None of the isolated Klebsiella pneumoniae was inhibited by the antimicrobial onslaught of nitrofurantoin. Table 4: Antibiogram of the Isolated Klebsiella Pneumoniae Isolates GN OF AMP N PF CT C AU CIP CM K11 S S R R S S R R R R K2 R S S R S S R R S R K3 S R R R S R R R R R K14 S S R R S S R S R R K5 R R R R R R R R R R K6 S R R R R R R R R S K10 S R R R S S R R S R K12 S R R R R R R S S R K13 S S R R S R R R R R K9 S S S R S R R S R R K15 S S R R R R S S S R K16 S S R R S R R R S S K17 S S R R R R S R R R K18 R R R R S R R S S R K1 R S R R R S R R R R Keys: K = Klebsiella Pneumoniae, R = Resistant, S = Susceptible, GN = Gentamicin (R= 12, S= 15) OF = Ofloxacin (R= 12, S= 16), AMP = Ampicillin (R= 13, S= 17) N = Nitrofurantoin (R= 14, S= 17), PF = Perfloxacin (R= 12, S= 17) CT = Ceftriaxone (R= 19, S= 23), C = Chloramphenicol (R= 12, S= 18) AU = Augmentin (R= 13, S= 18) CIP = Ciprofloxacin (R= 15, S= 21) CM = Clarithromycin (R= 13, S= 18) Discussion Klebsiella pneumoniae is a nosocomial pathogen as well as a normal microflora of the gut of humans and animals; and this organism is implicated as a causative agent in human pneumonia infections as well as in other upper respiratory tract infections [4, 5, 6]. Antimicrobial resistance is a natural phenomenon but it can be contained and averted through proper drug prescription and appropriate detection of their emergence and spread in environmental microbiota. The economic cost of antimicrobial resistance goes beyond the morbidity and mortality associated with it; it also includes the loss of efficacy or ineffectiveness of some available antimicrobial agents [6, 8, 10, 11]. In this study, the prevalence and antimicrobial susceptibility profile of Klebsiella pneumoniae isolates bacteriologically recovered from the urine samples of school children in a local primary school in Abakaliki, Nigeria was analyzed. Out of the 25 urine samples analyzed in this study, a total of 15 (60%) isolates of Klebsiella pneumoniae was bacteriologically recovered, and this indicates a possible bacterial infection of the school children in this school used in our study. According to Podschun et al. [6], Klebsiella pneumoniae is a bacterial organism that is ubiquitously found in the environment including surface waters; and the bacterium is found in animals and humans as an important community/nosocomial pathogen that may harbour antibiotic resistance traits [6, 7, 11]. The prevalence of Klebsiella pneumoniae in our study compares favorably page 4 of 6

5 Healthy School Children. 1:1. with a previous report by Omonigho et al. [12] and Ebie et al. [13] who reported that Klebsiella pneumoniae was the most occurring Enterobacteriaceae found in cases of urinary tract infections (UTI) of urine samples of patients analyzed from both the community and hospital environment. The result of the antimicrobial susceptibility testing shows that the Klebsiella pneumoniae were highly resistant to ampicillin, chloramphenicol, clarithromycin, ceftriaxone, augmentin and ciprofloxacin. This is similar to the reports of Adeyemo et al. [14] who also reported in their study that enteric organisms particularly Klebsiella pneumoniae are multidrug resistant in nature. Most notably, all the Klebsiella pneumoniae isolates were completely resistant to nitrofurantoin (100%), which is used clinically for the treatment of urinary tract infections (UTIs). This is in contrast to the work of Chikere et al. [15] who reported sensitivities of enteric organisms including Klebsiella pneumoniae to nitrofurantoin. In their study, Okonko et al. [16] reported sensitivities of enteric organisms including Klebsiella pneumoniae to nitrofurantoin; and they also recommended the use of nitrofurantoin for the treatment of UTIs especially when treatment is necessary. However, the Klebsiella pneumoniae isolates recovered in this study showed appreciable sensitivity to gentamicin and ofloxacin, which are found in the aminoglycoside and fluoroquinolone families of antibiotics respectively. Conclusively, our study shows that Klebsiella pneumoniae isolates from community samples are notoriously resistant to some commonly used antibiotics especially to nitrofurantoin to which all the Klebsiella pneumoniae isolates showed reduced susceptibility. We recommend the continuous screening of samples from the nonhospital environment for possible emergence and spread of drug resistant bacteria that may be responsible for some community acquired infections. Further molecular characterization of the Klebsiella pneumoniae isolated in this study is required for deciphering the genetic determinants responsible for the multidrug resistant nature of the isolates. References 1. Ejikeugwu C, Esimone C, Iroha I, et al. (2017) Molecular Identification of MBL Genes blaimp-1 and blavim-1 in Escherichia coli Strains Isolated from Abattoir by Multiplex PCR Technique. Res J Microbiol 12: Ejikeugwu C, Ikegbunam M, Ugwu C, et al. (2013) Phenotypic Detection of Klebsiella pneumoniae Strains-Producing Extended Spectrum β-lactamase (ESBL) Enzymes. Scholars Acad J Biosci 1: Ejikeugwu C, Esimone C, Iroha I, et al. (2016) Phenotypic detection of AmpC beta-lactamase among anal Pseudomonas aeruginosa isolates in a Nigerian abattoir. Arch Clin Microbiol 7: Brooks GF, Butel JS, Morse SA (2004) Medical Microbiology, 23rd edition, McGraw Hill Publishers. USA Madigan MT, Martinko JM, Dunlap PV et al. (2009) Brock Biology of microorganisms, 12th edition, Pearson Benjamin Cummings Publishers. USA Podschun R, Pietsch S, Holler C, et al. (2001) Incidence of Klebsiella species in surface waters and their expression of virulence factors. Appl Environ Microbiol 67: Ogawa W, Li Dai-Wei, Yu P, et al. (2005) Multidrug resistance in Klebsiella pneumoniae MGH78578 and cloning of genes responsible for the resistance. Biol Pharm Bull 28: Jadhau S, Rabindranath M, Nageshawari G, et al. (2012) Increasing incidence of multidrug resistance Klebsiella pneumoniae infections in hospital and community settings. Int J Microbiol Res 4: Cheesbrough M (2006) Biochemical tests to identify bacteria. In: Laboratory practice in tropical countries. Cambridge Press Jean B. Patel, Robin Patel, Melvin P. W, et al. (2011) Clinical Laboratory Standard Institute (CLSI) Performance standards for antimicrobial disk susceptibility test. Fifteenth informational supplement. CLSI document, Wayne, USA M100-S Bush K, Jacoby GA (2010) Updated functional classification of β-lactamases. Antimicrob Agents Chemother 54: Omonigho SE, Obasi EE, Akukalia RN (2001) In-vitro Resistance of Urinary Isolates of Escherichia coli and Klebsiella species to Nalidixic Acid. Nigeria J Microbiol 15: Ebie MY, Kandakai-Olukemi YT, Ayanbadejo J, et al. (2001) Urinary Tract Infections in a Nigerian Military Hospital. Nigerian J Microbiol 15: Adeyemo AA, Gbadegesin RA, Onyemenen IN, et al. (1994) Urinary Tract Pathogens and anti-microbial sensitivity in children in Ibadan. Nigeria Annals of Trop Paediatrics 14: page 5 of 6

6 Healthy School Children. 1: Chikere CB, Chikere BO, Omoni VT (2008) Antibiogram of clinical isolates from a hospital in Nigeria. African J Biotechnol 7: Okonko IO, Soleye FA, Amusan TA, et al. (2009) Incidence of Multidrug resistance (MDR) organisms in Abeokuta, Southwestern Nigeria. Global Journal of Pharmacology 3: Citation: Ejikeugwu PC (2017) Frequency and Antibiogram of Urinary Isolates of Klebsiella Pneumoniae Isolated from Urine Samples of Apparently Healthy School Children. 1:1. page 6 of 6

Bacteriological and antibiogram of AmpC producing Enterobacteriaceae isolated from abattoir.

Bacteriological and antibiogram of AmpC producing Enterobacteriaceae isolated from abattoir. Research Article http://www.alliedacademies.org/microbiology-current-research/ Bacteriological and antibiogram of AmpC producing Enterobacteriaceae isolated from abattoir. Ejikeugwu C 1*, Nworie O. 2,

More information

Prevalence of Metallo-Beta-Lactamase Producing Pseudomonas aeruginosa and its antibiogram in a tertiary care centre

Prevalence of Metallo-Beta-Lactamase Producing Pseudomonas aeruginosa and its antibiogram in a tertiary care centre International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 4 Number 9 (2015) pp. 952-956 http://www.ijcmas.com Original Research Article Prevalence of Metallo-Beta-Lactamase

More information

Nature and Science 2016;14(11) State, Nigeria. Nigeria. Nigeria

Nature and Science 2016;14(11)  State, Nigeria. Nigeria. Nigeria Antibiogram and Detection of Metallo-Beta-Lactamase (MBL) positive Escherichia coli isolates from abattoir Ejikeugwu Chika* 1, Edeh Chijioke 1, Iroha Ifeanyichukwu 1, Orji Jerry 1, Eluu Stanley 2, Ugbo

More information

Prevalence of Extended Spectrum Beta- Lactamase Producers among Various Clinical Samples in a Tertiary Care Hospital: Kurnool District, India

Prevalence of Extended Spectrum Beta- Lactamase Producers among Various Clinical Samples in a Tertiary Care Hospital: Kurnool District, India International Journal of Current Microbiology and Applied Sciences ISSN: 319-77 Volume Number (17) pp. 57-3 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/1.5/ijcmas.17..31

More information

Antibiotic Susceptibility Pattern of Vibrio cholerae Causing Diarrohea Outbreaks in Bidar, North Karnataka, India

Antibiotic Susceptibility Pattern of Vibrio cholerae Causing Diarrohea Outbreaks in Bidar, North Karnataka, India International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 4 Number 9 (2015) pp. 957-961 http://www.ijcmas.com Original Research Article Antibiotic Susceptibility Pattern

More information

Comparison of Antibiotic Resistance and Sensitivity with Reference to Ages of Elders

Comparison of Antibiotic Resistance and Sensitivity with Reference to Ages of Elders Daffodil International University Institutional Repository DIU Journal of Science and Technology Volume 10, Issue 1-2, July 2015 2016-06-16 Comparison of Antibiotic Resistance and Sensitivity with Reference

More information

RETROSPECTIVE STUDY OF GRAM NEGATIVE BACILLI ISOLATES AMONG DIFFERENT CLINICAL SAMPLES FROM A DIAGNOSTIC CENTER OF KANPUR

RETROSPECTIVE STUDY OF GRAM NEGATIVE BACILLI ISOLATES AMONG DIFFERENT CLINICAL SAMPLES FROM A DIAGNOSTIC CENTER OF KANPUR Original article RETROSPECTIVE STUDY OF GRAM NEGATIVE BACILLI ISOLATES AMONG DIFFERENT CLINICAL SAMPLES FROM A DIAGNOSTIC CENTER OF KANPUR R.Sujatha 1,Nidhi Pal 2, Deepak S 3 1. Professor & Head, Department

More information

ESBL Producers An Increasing Problem: An Overview Of An Underrated Threat

ESBL Producers An Increasing Problem: An Overview Of An Underrated Threat ESBL Producers An Increasing Problem: An Overview Of An Underrated Threat Hicham Ezzat Professor of Microbiology and Immunology Cairo University Introduction 1 Since the 1980s there have been dramatic

More information

ANTIBIOTIC SENSITIVITY PATTERN OF YERSINIA ENTEROCOLITICA ISOLATED FROM MILK AND DAIRY PRODUCTS*

ANTIBIOTIC SENSITIVITY PATTERN OF YERSINIA ENTEROCOLITICA ISOLATED FROM MILK AND DAIRY PRODUCTS* Short Communication ANTIBIOTIC SENSITIVITY PATTERN OF YERSINIA ENTEROCOLITICA ISOLATED FROM MILK AND DAIRY PRODUCTS* T.R.Pugazhenthi 1, A. Elango 2, C. Naresh Kumar 3, B. Dhanalakshmi 4 and A. Bharathidhasan

More information

Detection of ESBL Producing Gram Negative Uropathogens and their Antibiotic Resistance Pattern from a Tertiary Care Centre, Bengaluru, India

Detection of ESBL Producing Gram Negative Uropathogens and their Antibiotic Resistance Pattern from a Tertiary Care Centre, Bengaluru, India ISSN: 2319-7706 Volume 4 Number 12 (2015) pp. 578-583 http://www.ijcmas.com Original Research Article Detection of ESBL Producing Gram Negative Uropathogens and their Antibiotic Resistance Pattern from

More information

APPENDIX III - DOUBLE DISK TEST FOR ESBL

APPENDIX III - DOUBLE DISK TEST FOR ESBL Policy # MI\ANTI\04\03\v03 Page 1 of 5 Section: Antimicrobial Susceptibility Testing Manual Subject Title: Appendix III - Double Disk Test for ESBL Issued by: LABORATORY MANAGER Original Date: January

More information

Lab Exercise: Antibiotics- Evaluation using Kirby Bauer method.

Lab Exercise: Antibiotics- Evaluation using Kirby Bauer method. Lab Exercise: Antibiotics- Evaluation using Kirby Bauer method. OBJECTIVES 1. Compare the antimicrobial capabilities of different antibiotics. 2. Compare effectiveness of with different types of bacteria.

More information

European Committee on Antimicrobial Susceptibility Testing

European Committee on Antimicrobial Susceptibility Testing European Committee on Antimicrobial Susceptibility Testing Routine and extended internal quality control as recommended by EUCAST Version 5.0, valid from 015-01-09 This document should be cited as "The

More information

Susceptibility and Detection of Extended Spectrum β-lactamase Enzymes from Otitis Media Pathogens

Susceptibility and Detection of Extended Spectrum β-lactamase Enzymes from Otitis Media Pathogens American Journal of Infectious Diseases 9 (1): 24-29, 2013 ISSN: 1553-6203 2013 Science Publication doi:10.3844/ajidsp.2013.24.29 Published Online 9 (1) 2013 (http://www.thescipub.com/ajid.toc) Susceptibility

More information

a. 379 laboratories provided quantitative results, e.g (DD method) to 35.4% (MIC method) of all participants; see Table 2.

a. 379 laboratories provided quantitative results, e.g (DD method) to 35.4% (MIC method) of all participants; see Table 2. AND QUANTITATIVE PRECISION (SAMPLE UR-01, 2017) Background and Plan of Analysis Sample UR-01 (2017) was sent to API participants as a simulated urine culture for recognition of a significant pathogen colony

More information

EXTENDED-SPECTRUM BETA-LACTAMASE (ESBL) TESTING

EXTENDED-SPECTRUM BETA-LACTAMASE (ESBL) TESTING EXTENDED-SPECTRUM BETA-LACTAMASE (ESBL) TESTING CHN61: EXTENDED-SPECTRUM BETA-LACTAMASE (ESBL) TESTING 1.1 Introduction A common mechanism of bacterial resistance to beta-lactam antibiotics is the production

More information

Intrinsic, implied and default resistance

Intrinsic, implied and default resistance Appendix A Intrinsic, implied and default resistance Magiorakos et al. [1] and CLSI [2] are our primary sources of information on intrinsic resistance. Sanford et al. [3] and Gilbert et al. [4] have been

More information

Defining Extended Spectrum b-lactamases: Implications of Minimum Inhibitory Concentration- Based Screening Versus Clavulanate Confirmation Testing

Defining Extended Spectrum b-lactamases: Implications of Minimum Inhibitory Concentration- Based Screening Versus Clavulanate Confirmation Testing Infect Dis Ther (2015) 4:513 518 DOI 10.1007/s40121-015-0094-6 BRIEF REPORT Defining Extended Spectrum b-lactamases: Implications of Minimum Inhibitory Concentration- Based Screening Versus Clavulanate

More information

Antimicrobial Stewardship Strategy: Antibiograms

Antimicrobial Stewardship Strategy: Antibiograms Antimicrobial Stewardship Strategy: Antibiograms A summary of the cumulative susceptibility of bacterial isolates to formulary antibiotics in a given institution or region. Its main functions are to guide

More information

A retrospective analysis of urine culture results issued by the microbiology department, Teaching Hospital, Karapitiya

A retrospective analysis of urine culture results issued by the microbiology department, Teaching Hospital, Karapitiya A retrospective analysis of urine culture results issued by the microbiology department, Teaching Hospital, Karapitiya LU Edirisinghe 1, D Vidanagama 2 1 Senior Registrar in Medicine, 2 Consultant Microbiologist,

More information

Antibiotic Susceptibility of Common Bacterial Pathogens in Canine Urinary Tract Infections

Antibiotic Susceptibility of Common Bacterial Pathogens in Canine Urinary Tract Infections Vol.1 No.2 Oct-Dec 2013 ISSN : 2321-6387 Antibiotic Susceptibility of Common Bacterial Pathogens in Canine Urinary Tract Infections S. Yogeshpriya*, Usha N.Pillai, S. Ajithkumar and N. Madhavan Unny Department

More information

GENERAL NOTES: 2016 site of infection type of organism location of the patient

GENERAL NOTES: 2016 site of infection type of organism location of the patient GENERAL NOTES: This is a summary of the antibiotic sensitivity profile of clinical isolates recovered at AIIMS Bhopal Hospital during the year 2016. However, for organisms in which < 30 isolates were recovered

More information

Occurrence of Multi-drug Resistant Gram Negative Bacteria from Poultry and Poultry Products sold in Abakaliki Metropolis, Nigeria

Occurrence of Multi-drug Resistant Gram Negative Bacteria from Poultry and Poultry Products sold in Abakaliki Metropolis, Nigeria 2016 Pearl Research Journals Journal of Agricultural Science and Food Technology Vol. 2 (7), pp. 119-124, August, 2016 ISSN: 2465-7522 Full Length Research Paper http://pearlresearchjournals.org/journals/jasft/index.html

More information

EUCAST recommended strains for internal quality control

EUCAST recommended strains for internal quality control EUCAST recommended strains for internal quality control Escherichia coli Pseudomonas aeruginosa Staphylococcus aureus Enterococcus faecalis Streptococcus pneumoniae Haemophilus influenzae ATCC 59 ATCC

More information

Version 1.01 (01/10/2016)

Version 1.01 (01/10/2016) CHN58: ANTIMICROBIAL SUSCEPTIBILITY TESTING (CLSI) 1.0 PURPOSE / INTRODUCTION: 1.1 Introduction Antimicrobial susceptibility tests are performed in order to determine whether a pathogen is likely to be

More information

Florida Health Care Association District 2 January 13, 2015 A.C. Burke, MA, CIC

Florida Health Care Association District 2 January 13, 2015 A.C. Burke, MA, CIC Florida Health Care Association District 2 January 13, 2015 A.C. Burke, MA, CIC 11/20/2014 1 To describe carbapenem-resistant Enterobacteriaceae. To identify laboratory detection standards for carbapenem-resistant

More information

Study of drug resistance pattern of principal ESBL producing urinary isolates in an urban hospital setting in Eastern India

Study of drug resistance pattern of principal ESBL producing urinary isolates in an urban hospital setting in Eastern India Research article Study of drug resistance pattern of principal ESBL producing urinary isolates in an urban hospital setting in Eastern India Mitali Chatterjee, 1 M. Banerjee, 1 S. Guha, 2 A.Lahiri, 3 K.Karak

More information

Bacterial Pathogens in Urinary Tract Infection and Antibiotic Susceptibility Pattern from a Teaching Hospital, Bengaluru, India

Bacterial Pathogens in Urinary Tract Infection and Antibiotic Susceptibility Pattern from a Teaching Hospital, Bengaluru, India ISSN: 2319-7706 Volume 4 Number 11 (2015) pp. 731-736 http://www.ijcmas.com Original Research Article Bacterial Pathogens in Urinary Tract Infection and Antibiotic Susceptibility Pattern from a Teaching

More information

Multiple Antibiotic Resistance, Antibiogram and Phenotypic Detection of Metallo-Beta- Poultry Origin. Abstract. Introduction

Multiple Antibiotic Resistance, Antibiogram and Phenotypic Detection of Metallo-Beta- Poultry Origin. Abstract. Introduction Research Article imedpub Journals www.imedpub.com Journal of Applied Microbiology and Biochemistry DOI: 10.21767/2576-1412.100015 Multiple Antibiotic Resistance, Antibiogram and Phenotypic Detection of

More information

Cockroaches (Periplaneta americana) as possible reservoirs of extended spectrum beta-lactamase (ESBL)-producing pathogenic bacteria

Cockroaches (Periplaneta americana) as possible reservoirs of extended spectrum beta-lactamase (ESBL)-producing pathogenic bacteria International Journal of Research in Biosciences Vol. 6 Issue 3, pp. (41-49), July 2017 Available online at http://www.ijrbs.in ISSN 2319-2844 Research Paper Cockroaches (Periplaneta americana) as possible

More information

International Journal of Pharma and Bio Sciences ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF ESBL PRODUCING GRAM NEGATIVE BACILLI ABSTRACT

International Journal of Pharma and Bio Sciences ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF ESBL PRODUCING GRAM NEGATIVE BACILLI ABSTRACT Research Article Microbiology International Journal of Pharma and Bio Sciences ISSN 0975-6299 ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF ESBL PRODUCING GRAM NEGATIVE BACILLI * PRABHAKAR C MAILAPUR, DEEPA

More information

2015 Antimicrobial Susceptibility Report

2015 Antimicrobial Susceptibility Report Gram negative Sepsis Outcome Programme (GNSOP) 2015 Antimicrobial Susceptibility Report Prepared by A/Professor Thomas Gottlieb Concord Hospital Sydney Jan Bell The University of Adelaide Adelaide On behalf

More information

Isolation, identification and antimicrobial susceptibility pattern of uropathogens isolated at a tertiary care centre

Isolation, identification and antimicrobial susceptibility pattern of uropathogens isolated at a tertiary care centre International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 4 Number 10 (2015) pp. 951-955 http://www.ijcmas.com Original Research Article Isolation, identification and antimicrobial

More information

JOURNAL OF INTERNATIONAL ACADEMIC RESEARCH FOR MULTIDISCIPLINARY Impact Factor 1.625, ISSN: , Volume 3, Issue 4, May 2015

JOURNAL OF INTERNATIONAL ACADEMIC RESEARCH FOR MULTIDISCIPLINARY Impact Factor 1.625, ISSN: , Volume 3, Issue 4, May 2015 PHENOTYPIC DETECTION OF FAECAL CARRIAGE EXTENDED SPECTRUM BETA LACTAMASE PRODUCING KLEBSIELLA PNEUMONIAE IN HILLA CITY Dr. FATIMA MOEEN ABBAS* *Dept. of Biology, College of Sciences for Women, University

More information

BACTERIOLOGICALL STUDY OF MICROORGANISMS ON MOBILES AND STETHOSCOPES USED BY HEALTH CARE WORKERS IN EMERGENCY AND ICU S

BACTERIOLOGICALL STUDY OF MICROORGANISMS ON MOBILES AND STETHOSCOPES USED BY HEALTH CARE WORKERS IN EMERGENCY AND ICU S Research Article Harika A,, 2013; Volume 2(3): 290-297 ISSN: 2277-8713 BACTERIOLOGICALL STUDY OF MICROORGANISMS ON MOBILES AND STETHOSCOPES USED BY HEALTH CARE WORKERS IN EMERGENCY AND ICU S HARIKAA A,

More information

Antimicrobial Susceptibility Patterns of Salmonella Typhi From Kigali,

Antimicrobial Susceptibility Patterns of Salmonella Typhi From Kigali, In the name of God Shiraz E-Medical Journal Vol. 11, No. 3, July 2010 http://semj.sums.ac.ir/vol11/jul2010/88030.htm Antimicrobial Susceptibility Patterns of Salmonella Typhi From Kigali, Rwanda. Ashok

More information

The Basics: Using CLSI Antimicrobial Susceptibility Testing Standards

The Basics: Using CLSI Antimicrobial Susceptibility Testing Standards The Basics: Using CLSI Antimicrobial Susceptibility Testing Standards Janet A. Hindler, MCLS, MT(ASCP) UCLA Health System Los Angeles, California, USA jhindler@ucla.edu 1 Learning Objectives Describe information

More information

Isolation of Urinary Tract Pathogens and Study of their Drug Susceptibility Patterns

Isolation of Urinary Tract Pathogens and Study of their Drug Susceptibility Patterns International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 5 Number 4 (2016) pp. 897-903 Journal homepage: http://www.ijcmas.com Original Research Article http://dx.doi.org/10.20546/ijcmas.2016.504.101

More information

Detection and antimicrobial susceptibility of some gram negative bacteria producing carbapenemases and extended spectrum β-lactamases

Detection and antimicrobial susceptibility of some gram negative bacteria producing carbapenemases and extended spectrum β-lactamases International Journal of Microbiology and Immunology Research Vol.2(6), pp. 064-069, November, 2013 Available online at http://www.apexjournal.org ISSN 2315-8743 2013 Apex Journal International Full Length

More information

Received:06 th June-2012 Revised: 10 th June-2012 Accepted: 13 th June-2012 Research article

Received:06 th June-2012 Revised: 10 th June-2012 Accepted: 13 th June-2012 Research article Received:06 th June-2012 Revised: 10 th June-2012 Accepted: 13 th June-2012 Research article EMERGENCE OF MULTI DRUG RESISTANT STRAINS OF E. COLI ISOLATED FROM URINARY TRACT INFECTION IN NAMAKKAL 1 P.

More information

Multiple drug resistance pattern in Urinary Tract Infection patients in Aligarh

Multiple drug resistance pattern in Urinary Tract Infection patients in Aligarh Multiple drug resistance pattern in Urinary Tract Infection patients in Aligarh Author(s): Asad U Khan and Mohd S Zaman Vol. 17, No. 3 (2006-09 - 2006-12) Biomedical Research 2006; 17 (3): 179-181 Asad

More information

Evaluation of a computerized antimicrobial susceptibility system with bacteria isolated from animals

Evaluation of a computerized antimicrobial susceptibility system with bacteria isolated from animals J Vet Diagn Invest :164 168 (1998) Evaluation of a computerized antimicrobial susceptibility system with bacteria isolated from animals Susannah K. Hubert, Phouc Dinh Nguyen, Robert D. Walker Abstract.

More information

Int.J.Curr.Microbiol.App.Sci (2017) 6(3):

Int.J.Curr.Microbiol.App.Sci (2017) 6(3): International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 3 (2017) pp. 891-895 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.603.104

More information

Acinetobacter species-associated infections and their antibiotic susceptibility profiles in Malaysia.

Acinetobacter species-associated infections and their antibiotic susceptibility profiles in Malaysia. Biomedical Research 12; 23 (4): 571-575 ISSN 97-938X Scientific Publishers of India Acinetobacter species-associated infections and their antibiotic susceptibility profiles in Malaysia. Nazmul MHM, Jamal

More information

Understanding the Hospital Antibiogram

Understanding the Hospital Antibiogram Understanding the Hospital Antibiogram Sharon Erdman, PharmD Clinical Professor Purdue University College of Pharmacy Infectious Diseases Clinical Pharmacist Eskenazi Health 5 Understanding the Hospital

More information

Background and Plan of Analysis

Background and Plan of Analysis ENTEROCOCCI Background and Plan of Analysis UR-11 (2017) was sent to API participants as a simulated urine culture for recognition of a significant pathogen colony count, to perform the identification

More information

ESBL- and carbapenemase-producing microorganisms; state of the art. Laurent POIREL

ESBL- and carbapenemase-producing microorganisms; state of the art. Laurent POIREL ESBL- and carbapenemase-producing microorganisms; state of the art Laurent POIREL Medical and Molecular Microbiology Unit Dept of Medicine University of Fribourg Switzerland INSERM U914 «Emerging Resistance

More information

World Journal of Pharmaceutical and Life Sciences WJPLS

World Journal of Pharmaceutical and Life Sciences WJPLS wjpls, 2017, Vol. 3, Issue 2, 119-123 Research Article ISSN 2454-2229 WJPLS www.wjpls.org SJIF Impact Factor: 4.223 PHENOTYPIC DETECTION OF METHICILLIN RESISTANCE IN PATHOGENIC STAPHYLOCOCCUS AUREUS BY

More information

Comparative Assessment of b-lactamases Produced by Multidrug Resistant Bacteria

Comparative Assessment of b-lactamases Produced by Multidrug Resistant Bacteria Comparative Assessment of b-lactamases Produced by Multidrug Resistant Bacteria Juhee Ahn Department of Medical Biomaterials Engineering Kangwon National University October 23, 27 Antibiotic Development

More information

GeNei TM. Antibiotic Sensitivity. Teaching Kit Manual KT Revision No.: Bangalore Genei, 2007 Bangalore Genei, 2007

GeNei TM. Antibiotic Sensitivity. Teaching Kit Manual KT Revision No.: Bangalore Genei, 2007 Bangalore Genei, 2007 GeNei Bacterial Antibiotic Sensitivity Teaching Kit Manual Cat No. New Cat No. KT68 106333 Revision No.: 00180705 CONTENTS Page No. Objective 3 Principle 3 Kit Description 4 Materials Provided 5 Procedure

More information

International Journal of Health Sciences and Research ISSN:

International Journal of Health Sciences and Research  ISSN: International Journal of Health Sciences and Research www.ijhsr.org ISSN: 2249-9571 Original Research Article Phenotypic Characterization of Extended Spectrum Beta-Lactamase (ESBL) - Producing Clinical

More information

ALARMING RATES OF PREVALENCE OF ESBL PRODUCING E. COLI IN URINARY TRACT INFECTION CASES IN A TERTIARY CARE NEUROSPECIALITY HOSPITAL

ALARMING RATES OF PREVALENCE OF ESBL PRODUCING E. COLI IN URINARY TRACT INFECTION CASES IN A TERTIARY CARE NEUROSPECIALITY HOSPITAL ALARMING RATES OF PREVALENCE OF ESBL PRODUCING E. COLI IN URINARY TRACT INFECTION CASES IN A TERTIARY CARE NEUROSPECIALITY HOSPITAL Pearl. A Prabal*,Sourav Maiti Institute of Neurosciences, Kolkata, India

More information

Int.J.Curr.Microbiol.App.Sci (2016) 5(12):

Int.J.Curr.Microbiol.App.Sci (2016) 5(12): International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 5 Number 12 (2016) pp. 644-649 Journal homepage: http://www.ijcmas.com Original Research Article http://dx.doi.org/10.20546/ijcmas.2016.512.071

More information

Antibiotic Reference Laboratory, Institute of Environmental Science and Research Limited (ESR); August 2017

Antibiotic Reference Laboratory, Institute of Environmental Science and Research Limited (ESR); August 2017 Antimicrobial susceptibility of Shigella, 2015 and 2016 Helen Heffernan and Rosemary Woodhouse Antibiotic Reference Laboratory, Institute of Environmental Science and Research Limited (ESR); August 2017

More information

Enterobacter aerogenes

Enterobacter aerogenes Enterobacter aerogenes Enterobacter sp. Enterobacter sp. Species: Enterobacter aerogenes Enterobacter agglomerans Enterobacter cloacae causes UTI, enterotoxigenic Often found in the normal intestinal flora,

More information

Aerobic bacterial infections in a burns unit of Sassoon General Hospital, Pune

Aerobic bacterial infections in a burns unit of Sassoon General Hospital, Pune Original article Aerobic bacterial infections in a burns unit of Sassoon General Hospital, Pune Patil P, Joshi S, Bharadwaj R. Department of Microbiology, B.J. Medical College, Pune, India. Corresponding

More information

Prevalence of Extended-spectrum β-lactamase Producing Enterobacteriaceae Strains in Latvia

Prevalence of Extended-spectrum β-lactamase Producing Enterobacteriaceae Strains in Latvia Prevalence of Extended-spectrum β-lactamase Producing Enterobacteriaceae Strains in Latvia Ruta Paberza 1, Solvita Selderiņa 1, Sandra Leja 1, Jelena Storoženko 1, Lilija Lužbinska 1, Aija Žileviča 2*

More information

Routine internal quality control as recommended by EUCAST Version 3.1, valid from

Routine internal quality control as recommended by EUCAST Version 3.1, valid from Routine internal quality control as recommended by EUCAST Version.1, valid from 01-01-01 Escherichia coli Pseudomonas aeruginosa Staphylococcus aureus Enterococcus faecalis Streptococcus pneumoniae Haemophilus

More information

Mechanisms and Pathways of AMR in the environment

Mechanisms and Pathways of AMR in the environment FMM/RAS/298: Strengthening capacities, policies and national action plans on prudent and responsible use of antimicrobials in fisheries Final Workshop in cooperation with AVA Singapore and INFOFISH 12-14

More information

2012 ANTIBIOGRAM. Central Zone Former DTHR Sites. Department of Pathology and Laboratory Medicine

2012 ANTIBIOGRAM. Central Zone Former DTHR Sites. Department of Pathology and Laboratory Medicine 2012 ANTIBIOGRAM Central Zone Former DTHR Sites Department of Pathology and Laboratory Medicine Medically Relevant Pathogens Based on Gram Morphology Gram-negative Bacilli Lactose Fermenters Non-lactose

More information

Title: N-Acetylcysteine (NAC) Mediated Modulation of Bacterial Antibiotic

Title: N-Acetylcysteine (NAC) Mediated Modulation of Bacterial Antibiotic AAC Accepts, published online ahead of print on June 00 Antimicrob. Agents Chemother. doi:0./aac.0070-0 Copyright 00, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights

More information

Antibiotic Resistance In Clinical Isolates Of Pseudomonas Aeruginosa In Enugu And Abakaliki, Nigeria

Antibiotic Resistance In Clinical Isolates Of Pseudomonas Aeruginosa In Enugu And Abakaliki, Nigeria ISPUB.COM The Internet Journal of Infectious Diseases Volume 7 Number 1 Antibiotic Resistance In Clinical Isolates Of Pseudomonas Aeruginosa In Enugu And Abakaliki, Nigeria E Amadi, P Uzoaru, I Orji, A

More information

PROTOCOL for serotyping and antimicrobial susceptibility testing of Salmonella test strains

PROTOCOL for serotyping and antimicrobial susceptibility testing of Salmonella test strains PROTOCOL for serotyping and antimicrobial susceptibility testing of Salmonella test strains 1 INTRODUCTION... 1 2 OBJECTIVES... 2 3 OUTLINE OF THE EQAS 2017... 2 3.1 Shipping, receipt and storage of strains...

More information

Helen Heffernan and Rosemary Woodhouse Antibiotic Reference Laboratory

Helen Heffernan and Rosemary Woodhouse Antibiotic Reference Laboratory METHODS USED IN NEW ZEALAND DIAGNOSTIC LABORATORIES TO IDENTIFY AND REPORT EXTENDED-SPECTRUM β-lactamase- PRODUCING ENTEROBACTERIACEAE by Helen Heffernan and Rosemary Woodhouse Antibiotic Reference Laboratory

More information

Detection of Metallo-β-lactamase (MBL) among Carbapenem-Resistant Gram-Negative Bacteria from Rectal Swabs of Cow and Cloacae Swabs of Poultry Birds

Detection of Metallo-β-lactamase (MBL) among Carbapenem-Resistant Gram-Negative Bacteria from Rectal Swabs of Cow and Cloacae Swabs of Poultry Birds Original Article Detection of Metallo-β-lactamase (MBL) among Carbapenem-Resistant Gram-Negative Bacteria from Rectal Swabs of Cow and Cloacae Swabs of Poultry Birds Ejikeugwu Chika 1 *, Esimone Charles

More information

European Committee on Antimicrobial Susceptibility Testing

European Committee on Antimicrobial Susceptibility Testing European Committee on Antimicrobial Susceptibility Testing Routine and extended internal quality control for MIC determination and disk diffusion as recommended by EUCAST Version 8.0, valid from 018-01-01

More information

National Surveillance of Antimicrobial Resistance

National Surveillance of Antimicrobial Resistance National Surveillance of Antimicrobial Resistance Report to Ministry of Health by Sri Lanka College of Microbiologists SLCM ARSP & NLBSA Technical Committees December 2014 National Surveillance of Antimicrobial

More information

Available Online at International Journal of Pharmaceutical & Biological Archives 2011; 2(5): ORIGINAL RESEARCH ARTICLE

Available Online at  International Journal of Pharmaceutical & Biological Archives 2011; 2(5): ORIGINAL RESEARCH ARTICLE ISSN 0976 3333 Available Online at www.ijpba.info International Journal of Pharmaceutical & Biological Archives 2011; 2(5):1502-1508 ORIGINAL RESEARCH ARTICLE Screening of ESBL (Extended Spectrum of β

More information

Mechanism of antibiotic resistance

Mechanism of antibiotic resistance Mechanism of antibiotic resistance Dr.Siriwoot Sookkhee Ph.D (Biopharmaceutics) Department of Microbiology Faculty of Medicine, Chiang Mai University Antibiotic resistance Cross-resistance : resistance

More information

Mili Rani Saha and Sanya Tahmina Jhora. Department of Microbiology, Sir Salimullah Medical College, Mitford, Dhaka, Bangladesh

Mili Rani Saha and Sanya Tahmina Jhora. Department of Microbiology, Sir Salimullah Medical College, Mitford, Dhaka, Bangladesh Detection of extended spectrum beta-lactamase producing Gram-negative organisms: hospital prevalence and comparison of double disc synergy and E-test methods Mili Rani Saha and Sanya Tahmina Jhora Original

More information

Dr. C. MANIKANDAN, Director,

Dr. C. MANIKANDAN, Director, STUDY OF PREVALENCE AND ANTIMICROBIAL SUSCEPTIBILITIES OF BACTERIA AND FUNGI ISOLATED FROM PATIENTS WITH URINARY TRACT INFECTIONS IN PATTUKKOTTAI, TAMIL NADU, INDIA Dr. C. MANIKANDAN, Director, Gangasaras

More information

Detection and Quantitation of the Etiologic Agents of Ventilator Associated Pneumonia in Endotracheal Tube Aspirates From Patients in Iran

Detection and Quantitation of the Etiologic Agents of Ventilator Associated Pneumonia in Endotracheal Tube Aspirates From Patients in Iran Letter to the Editor Detection and Quantitation of the Etiologic Agents of Ventilator Associated Pneumonia in Endotracheal Tube Aspirates From Patients in Iran Mohammad Rahbar, PhD; Massoud Hajia, PhD

More information

EDUCATIONAL COMMENTARY - Methicillin-Resistant Staphylococcus aureus: An Update

EDUCATIONAL COMMENTARY - Methicillin-Resistant Staphylococcus aureus: An Update EDUCATIONAL COMMENTARY - Methicillin-Resistant Staphylococcus aureus: An Update Educational commentary is provided through our affiliation with the American Society for Clinical Pathology (ASCP). To obtain

More information

Original Article. Hossein Khalili a*, Rasool Soltani b, Sorrosh Negahban c, Alireza Abdollahi d and Keirollah Gholami e.

Original Article. Hossein Khalili a*, Rasool Soltani b, Sorrosh Negahban c, Alireza Abdollahi d and Keirollah Gholami e. Iranian Journal of Pharmaceutical Research (22), (2): 559-563 Received: January 2 Accepted: June 2 Copyright 22 by School of Pharmacy Shaheed Beheshti University of Medical Sciences and Health Services

More information

Available online at ISSN No:

Available online at  ISSN No: Available online at www.ijmrhs.com ISSN No: 2319-5886 International Journal of Medical Research & Health Sciences, 2017, 6(4): 36-42 Comparative Evaluation of In-Vitro Doripenem Susceptibility with Other

More information

Safe Patient Care Keeping our Residents Safe Use Standard Precautions for ALL Residents at ALL times

Safe Patient Care Keeping our Residents Safe Use Standard Precautions for ALL Residents at ALL times Safe Patient Care Keeping our Residents Safe 2016 Use Standard Precautions for ALL Residents at ALL times #safepatientcare Do bugs need drugs? Dr Deirdre O Brien Consultant Microbiologist Mercy University

More information

Original Article. Ratri Hortiwakul, M.Sc.*, Pantip Chayakul, M.D.*, Natnicha Ingviya, B.Sc.**

Original Article. Ratri Hortiwakul, M.Sc.*, Pantip Chayakul, M.D.*, Natnicha Ingviya, B.Sc.** Original Article In Vitro Activity of Cefminox and Other β-lactam Antibiotics Against Clinical Isolates of Extended- Spectrum-β-lactamase-Producing Klebsiella pneumoniae and Escherichia coli Ratri Hortiwakul,

More information

CONTAGIOUS COMMENTS Department of Epidemiology

CONTAGIOUS COMMENTS Department of Epidemiology VOLUME XXIII NUMBER 1 July 2008 CONTAGIOUS COMMENTS Department of Epidemiology Bugs and Drugs Elaine Dowell, SM (ASCP), Marti Roe SM (ASCP), Ann-Christine Nyquist MD, MSPH Are the bugs winning? The 2007

More information

VLLM0421c Medical Microbiology I, practical sessions. Protocol to topic J05

VLLM0421c Medical Microbiology I, practical sessions. Protocol to topic J05 Topic J05: Determination of susceptibility of bacteria to antimicrobial drugs, assessments of resistance factors For study: textbooks, www, keywords e. g. Diffusion disc test ; E-test ; dilution micromethod

More information

Protocol for Surveillance of Antimicrobial Resistance in Urinary Isolates in Scotland

Protocol for Surveillance of Antimicrobial Resistance in Urinary Isolates in Scotland Protocol for Surveillance of Antimicrobial Resistance in Urinary Isolates in Scotland Version 1.0 23 December 2011 General enquiries and contact details This is the first version (1.0) of the Protocol

More information

Principles and Practice of Antimicrobial Susceptibility Testing. Microbiology Technical Workshop 25 th September 2013

Principles and Practice of Antimicrobial Susceptibility Testing. Microbiology Technical Workshop 25 th September 2013 Principles and Practice of Antimicrobial Susceptibility Testing Microbiology Technical Workshop 25 th September 2013 Scope History Why Perform Antimicrobial Susceptibility Testing? How to Perform an Antimicrobial

More information

International Journal of Health Sciences and Research ISSN:

International Journal of Health Sciences and Research  ISSN: International Journal of Health Sciences and Research www.ijhsr.org ISSN: 2249-9571 Original Research Article Antibiotic Susceptibility Pattern of Pseudomonas Aeruginosa Isolated From Various Clinical

More information

Aerobic Bacterial Profile and Antimicrobial Susceptibility Pattern of Pus Isolates in a Tertiary Care Hospital in Hadoti Region

Aerobic Bacterial Profile and Antimicrobial Susceptibility Pattern of Pus Isolates in a Tertiary Care Hospital in Hadoti Region International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 5 (2017) pp. 2866-2873 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.605.326

More information

Bacteriological Profile and Antimicrobial Sensitivity of Wound Infections

Bacteriological Profile and Antimicrobial Sensitivity of Wound Infections Int.J.Curr.Microbiol.App.Sci (215) 4(12): 248-254 ISSN: 2319-776 Volume 4 Number 12 (215) pp. 248-254 http://www.ijcmas.com Original Research Article Bacteriological Profile and Antimicrobial Sensitivity

More information

Cipro for klebsiella uti

Cipro for klebsiella uti Cipro for klebsiella uti Search Can UTI be an effective treatment for Klebsiella Pneumoniae? It is safe or dangerous to use UTI while suffering from Klebsiella Pneumoniae? 87 discussions on Treato. instock

More information

Urinary Tract Infections in Hospitalized Newborns in Beheshti Hospital, Iran: A Retrospective Study

Urinary Tract Infections in Hospitalized Newborns in Beheshti Hospital, Iran: A Retrospective Study Original Article Vol. 24 No. 1 Urinary Tract Infections in Newborns in Beheshti Hospital:- Movahedian AH, et al. 7 Urinary Tract Infections in Hospitalized Newborns in Beheshti Hospital, Iran: A Retrospective

More information

ESCMID Online Lecture Library. by author

ESCMID Online Lecture Library. by author Quality Assurance of antimicrobial susceptibility testing Derek Brown EUCAST Scientific Secretary ESCMID Postgraduate Education Course, Linz, 17 September 2014 Quality Assurance The total process by which

More information

Research Article. Drug resistance pattern of Pseudomonas aeruginosa isolates at PIMS Hospital, Islamabad, Pakistan

Research Article. Drug resistance pattern of Pseudomonas aeruginosa isolates at PIMS Hospital, Islamabad, Pakistan Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2014, 6(11):715-719 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Drug resistance pattern of Pseudomonas aeruginosa

More information

Isolation of antibiotic producing Actinomycetes from soil of Kathmandu valley and assessment of their antimicrobial activities

Isolation of antibiotic producing Actinomycetes from soil of Kathmandu valley and assessment of their antimicrobial activities International Journal of Microbiology and Allied Sciences (IJOMAS) ISSN: 2382-5537 May 2016, 2(4):22-26 IJOMAS, 2016 Research Article Page: 22-26 Isolation of antibiotic producing Actinomycetes from soil

More information

Antibiotic Susceptibility Patterns of Streptococcus pneumoniae Isolated from the Nasopharyngeal Mucosa of Children in Enugu Metropolis, Nigeria

Antibiotic Susceptibility Patterns of Streptococcus pneumoniae Isolated from the Nasopharyngeal Mucosa of Children in Enugu Metropolis, Nigeria ISSN: 2319-7706 Volume 4 Number 9 (2015) pp. 1-9 http://www.ijcmas.com Original Research Article Antibiotic Susceptibility Patterns of Streptococcus pneumoniae Isolated from the Nasopharyngeal Mucosa of

More information

Aerobic bacteriological profile of urinary tract infections in a tertiary care hospital

Aerobic bacteriological profile of urinary tract infections in a tertiary care hospital ISSN: 2319-7706 Volume 3 Number 3 (2014) pp. 120-125 http://www.ijcmas.com Original Research Article Aerobic bacteriological profile of urinary tract infections in a tertiary care hospital V.Vijaya Swetha

More information

DISCLAIMER: ECHO Nevada emphasizes patient privacy and asks participants to not share ANY Protected Health Information during ECHO clinics.

DISCLAIMER: ECHO Nevada emphasizes patient privacy and asks participants to not share ANY Protected Health Information during ECHO clinics. DISCLAIMER: Video will be taken at this clinic and potentially used in Project ECHO promotional materials. By attending this clinic, you consent to have your photo taken and allow Project ECHO to use this

More information

Evaluation of antimicrobial activity of Salmonella species from various antibiotic

Evaluation of antimicrobial activity of Salmonella species from various antibiotic ISSN: 2347-3215 Volume 3 Number 8 (August-2015) pp. 51-55 www.ijcrar.com Evaluation of antimicrobial activity of Salmonella species from various antibiotic Shashi P. Jambhulkar 1 * and Arun B. Ingle 2

More information

Multi-drug resistant microorganisms

Multi-drug resistant microorganisms Multi-drug resistant microorganisms Arzu TOPELI Director of MICU Hacettepe University Faculty of Medicine, Ankara-Turkey Council Member of WFSICCM Deaths in the US declined by 220 per 100,000 with the

More information

Antimicrobial Resistance Trends in the Province of British Columbia

Antimicrobial Resistance Trends in the Province of British Columbia 655 West 12th Avenue Vancouver, BC V5Z 4R4 Tel 604.707.2443 Fax 604.707.2441 www.bccdc.ca Antimicrobial Resistance Trends in the Province of British Columbia 2013 Prepared by the Do Bugs Need Drugs? Program

More information

Suggestions for appropriate agents to include in routine antimicrobial susceptibility testing

Suggestions for appropriate agents to include in routine antimicrobial susceptibility testing Suggestions for appropriate agents to include in routine antimicrobial susceptibility testing These suggestions are intended to indicate minimum sets of agents to test routinely in a diagnostic laboratory

More information

Nova Journal of Medical and Biological Sciences Page: 1

Nova Journal of Medical and Biological Sciences Page: 1 Nova Explore Publications Nova Journal of Medical and Biological Sciences Vol. 3(1), 2014:1-5 PII: S2292793X1400003-3 www.novaexplore.com Multidrug resistance of Enterobacter Aerogenes isolated from bovine

More information

2017 Antibiogram. Central Zone. Alberta Health Services. including. Red Deer Regional Hospital. St. Mary s Hospital, Camrose

2017 Antibiogram. Central Zone. Alberta Health Services. including. Red Deer Regional Hospital. St. Mary s Hospital, Camrose 2017 Antibiogram Central Zone Alberta Health Services including Red Deer Regional Hospital St. Mary s Hospital, Camrose Introduction This antibiogram is a cumulative report of the antimicrobial susceptibility

More information

Study of Bacteriological Profile of Corneal Ulcers in Patients Attending VIMS, Ballari, India

Study of Bacteriological Profile of Corneal Ulcers in Patients Attending VIMS, Ballari, India International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 5 Number 7 (2016) pp. 200-205 Journal homepage: http://www.ijcmas.com Original Research Article http://dx.doi.org/10.20546/ijcmas.2016.507.020

More information

EARS Net Report, Quarter

EARS Net Report, Quarter EARS Net Report, Quarter 4 213 March 214 Key Points for 213* Escherichia coli: The proportion of patients with invasive infections caused by E. coli producing extended spectrum β lactamases (ESBLs) increased

More information