The Basics: Using CLSI Antimicrobial Susceptibility Testing Standards

Save this PDF as:
Size: px
Start display at page:

Download "The Basics: Using CLSI Antimicrobial Susceptibility Testing Standards"

Transcription

1 The Basics: Using CLSI Antimicrobial Susceptibility Testing Standards Janet A. Hindler, MCLS, MT(ASCP) UCLA Health System Los Angeles, California, USA 1

2 Learning Objectives Describe information contained in M02 and M07 and identify how these documents differ from the M100 tables. List ways to identify new information contained in M100. Discuss how M02, M07, and M100 can aid development of a quality program for antimicrobial susceptibility testing (AST) and reporting in clinical and public health laboratories. Discuss several procedural details of disk diffusion testing that could lead to errors if not performed as recommended. 2

3 The CLSI Subcommittee on Antimicrobial Susceptibility Testing develops AST standards. The subcommittee includes experts in infectious diseases, pharmaceuticals, and clinical laboratory practice. 3

4 Six Important Points to Ensure Quality AST Results 1. Only test bacteria that are likely to be causing an infection. (Not addressed in CLSI AST standards) 2. Use a standardized testing method. 3. Test and report antimicrobial agents appropriate for the organism and infection site. 4. Perform quality control (QC) routinely to ensure the AST is working properly. 5. Report results in a clear and concise manner so the physician will understand them. 6. Communicate highly significant findings (eg, multidrug resistant organisms) to appropriate resources (eg, infection control) in a timely manner. 4

5 CLSI AST Standards for Routine AST The Tables 2013 Disk Diffusion 2012 MIC MIC

6 CLSI AST Standards Standards that describe how to perform tests Disk diffusion: M02-A11 (2012)* Minimal inhibitory concentrations (MICs) (reference broth and agar dilution): M07-A9 (2012)* M100-S23 tables (2013) Suggested drugs to test/report Interpretive criteria (breakpoints) QC ranges * M02, M07 updated every three years M100 updated at least annually 6

7 US Clinical Laboratories and CLSI AST Standards CLSI publishes standard consensus reference methods. Clinical laboratories can use: CLSI test method as written Method that performs comparably to CLSI reference method (eg, US Food and Drug Administration [FDA] cleared diagnostic AST device) Each laboratory must customize testing and reporting to meet the needs of their facilities. Must interact with stakeholders (Infectious Diseases, Pharmacy, Infection Control, etc.) 7

8 Clinical Laboratory Can Use a CLSI Reference Method Disk diffusion (Kirby-Bauer) Broth microdilution (or agar dilution) MIC 8

9 or a Commercial System 9

10 If using a commercial AST system, follow the manufacturer s instructions precisely! 10

11 M02 and M07 Describe Steps for Performing AST Select fresh isolated colonies from agar plate. Prepare inoculum suspension. Standardize turbidity of inoculum suspension. Inoculate plate/tray/panel. Incubate. Read results. Interpret results. Confirm results. Report results. Follow timing rules! 11

12 M02-A11 Partial Table of Contents (similar for M07-A9) 12

13 Two Most Critical Steps in Performing Disk Diffusion Test Inoculum preparation M02-A11, Section 8 Examining zones of inhibition M02-A11, Section

14 Prepare inoculum Suspension. Select fresh ( 24-hour) colonies. Example: Direct colony suspension method 0.5 McFarland standard 14

15 Mix well. 0.5 McFarland standard Standardize inoculum suspension (manually or spectrophotometrically). 15

16 Swab plate. Remove sample. 16

17 Incubate overnight. Add disks. 17

18 Measuring Zones (1) Transmitted Light Reflected Light 18

19 Measuring Zones (2) Measure zone diameter using reflected light except for: Staphylococcus spp. linezolid Enterococcus spp. vancomycin Use transmitted light for these only and do not ignore ANY growth! 19

20 Measuring Zones (3) When using reflected light: Ignore miniscule colonies at zone s edge Proteus spp. ignore swarm within zone Trimethoprim, sulfonamides disregard slight growth (look for 80% reduction of the lawn of growth). 20

21 Measuring Zones (4) Proteus spp. ignore swarm 21

22 Measuring Zones (5) Measure zone of complete inhibition, except trimethoprimsulfamethoxazole and sulfonamides (ignore fine growth within obvious zone). 22

23 Measuring Zones (6) P. aeruginosa resistant subpopulation E. coli contaminated with enterococci repeat test Do not ignore colonies within zone. 23

24 Measuring Zones (7) Measure zone of growth NOT zone of hemolysis when using bloodsupplemented media. 24

25 Poor Growth (need confluent lawn) 25

26 M100-S23 Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement 26

27 Summary of Changes 27

28 The M100 Tables (1) Instructions for use of tables Drugs to test/report Table 1A nonfastidious bacteria Table 1B fastidious bacteria Table 1C anaerobes Interpretive criteria (breakpoints), examples Table 2A Enterobacteriaceae Table 2G Streptococcus pneumoniae Table 2J Anaerobes Supplemental tables, example Table 2D Supplemental Table 1. Screening Tests for High-Level Aminoglycoside Resistance (HLAR) and Vancomycin MIC 8 μg/ml in Enterococcus spp. 28

29 The M100 Tables (2) QC ranges Tables 3A 3B: disk diffusion Tables 4A 4E: MIC Other Tables antimicrobial solution/media preparation Appendixes, examples Patient results that should be confirmed Intrinsic resistance profiles of common species Anaerobes cumulative antibiograms Antimicrobial agent glossary 29

30 Instructions for Use of Tables 30

31 Instructions for us of Tables Strategy for selecting drugs to test/report (and selective reporting) Definitions of susceptible (S), intermediate (I), resistant (R), nonsusceptible (NS) Definitions of Warning and Therapy comments List of screening tests More 31

32 M100 Tables 1A 1C Select drugs to test/report. Determine if one drug can predict results for another. Identify drugs that are dangerous to report. Add comments to report. 32

33 Group A Group B Table 1A. Drugs to Test/Report (1) 33

34 Group C Group U Do NOT report on isolates from sources other than urine! Table 1A. Drugs to Test/Report (2) 34

35 Selective (or Cascade) Reporting Report (first line) drugs with narrowest spectrum, lowest toxicity, lowest cost first. Report others if: Resistant to first line (primary) From specific body site 35

36 Selective Reporting Example Pseudomonas aeruginosa Cefepime Ciprofloxacin Gentamicin Piperacillin-tazobactam S S S S 36

37 Selective Reporting Example Pseudomonas aeruginosa Amikacin S Cefepime R Ciprofloxacin S Gentamicin R Meropenem S Piper-tazobactam R Tobramycin R Selectively reported 2. Amikacin selectively reported when gentamicin-r and tobramycin-r 3. Meropenem selectively reported when cefepime- R and piperacillintazobactam -R 1. Tobramycin selectively reported when gentamicin-r 37

38 Should We Use Selective Reporting? Yes, with approval of physicians Maybe not, if there are other ways to encourage prudent prescribing in our institution Each laboratory must decide if selective reporting is right for them and which drugs to selectively report on individual species or organism groups. 38

39 Table 1A Enterobacteriaceae Acinetobacter spp. OR No OR Drugs in boxes have similar spectrum of activity against organism group. If OR, you can extrapolate results for two drugs. If no OR, you cannot extrapolate. 39

40 Serratia marcescens OR Example Ampicillin Cefazolin Cefotaxime Gentamicin Trimethoprim-sulfa... R R R S S Cefotaxime-R S. marcescens are ceftriaxone-r. 40

41 No OR Example Imipenem-S Meropenem-R Laboratory reported imipenem S Patient treated with meropenem No OR between imipenem and meropenem for Acinetobacter spp Cannot extrapolate results Patient died Lesho E, Wortmann G, Moran K, Craft D. Fatal Acinetobacter baumannii infection with discordant carbapenem susceptibility. Clin Infest Dis. 2005;41(5):

42 * some drugs can be reliably tested by MIC method only Table 1A. Drugs to Test/Report 42

43 CLSI Warning Comments Some organism/antimicrobial combinations show activity in vitro (ie, they test S ) but are clinically ineffective. DO NOT report as Susceptible. 43

44 Drug/Bug Combinations That Do Not Work in Patient Even if S in Test Organisms Salmonella spp., Shigella spp. Oxacillin-R staphylococci Enterococcus spp. Do Not Report as S First- and second- generation cephalosporins cephamycins aminoglycosides any beta-lactam (except ceftaroline for S. aureus) aminoglycosides (except high conc) cephalosporins clindamycin trimethoprim-sulfamethoxazole 44

45 DO NOT Report on CSF Isolates Little or No Active Drug Gets into CSF Agents administered by oral route only First- and second- generation cephalosporins (except cefuroxime parenteral) and cephamycins Clindamycin Macrolides Tetracyclines Fluoroquinolones Abbreviation: CSF, cerebrospinal fluid. 45

46 Urine Isolates DO NOT report routinely on urine isolates. Azithromycin Chloramphenicol Clarithromycin Clindamycin Erythromycin Report ONLY on urine isolates. Drugs in Group U in M100-S23 Table 1A For example, nitrofurantoin. 46

47 How should we interpret results? Table 2A. Interpretive Criteria (Breakpoints) 47

48 48 Why might an antimicrobial agent be listed in Table 2 but not in Table 1? Drug may have a clinical indication for the organism group but is generally not appropriate for routine testing and reporting in United States. Listed as test/report group O (other) Drug may not be FDA-approved Listed as test/report group Inv (investigational) Table 2A. Interpretive Criteria (Breakpoints)

49 Some Organism-Drug Combinations Have Susceptible-Only Breakpoints S. pneumoniae Vancomycin MIC (µg/ml) (Note: to date, vancomycin NS has not been reported in S. pneumoniae) Susc Int Res Vancomycin Investigate any nonsusceptible (NS) result. Repeat identification and AST. Save isolate. Send to reference laboratory (test by CLSI MIC method). 49

50 Table 2C Supplemental Table 1. Screening Tests for S. aureus Group 50

51 How can we ensure accurate results? Table 3A. QC Organism Ranges 51

52 Appendix A. Confirm Patient s Results (all results should be confirmed but some require additional attention) 52

53 Additional Quality Control Tables QC testing frequency Tables 3C, 4F Troubleshooting guide Tables 3D, 4G Summary of QC strains and their use Appendix C 53

54 Appendix B. Intrinsic Resistance Enterobacteriaceae 54

55 Glossaries I and II Class, subclass, generic name Abbreviations: PO = per OS (oral); IM = intramuscular; IV = intravenous. 55

56 Glossaries of Antimicrobial Agents 56

57 Artificial Intelligence (eg, expert software) Helps to: Flag atypical/inconsistent results. Suggest confirmatory tests. Report appropriate drugs. Edit S or I results to R. Add comments to report. AST but still need informed clinical laboratory scientists! 57

58 CLSI Website Find information from AST meetings. Order CLSI AST products. subcommittee-on-ast/ 58

59 An online interactive searchable version of M100-S23 enables you to easily Access M100-S23 all you need is a Web connection. Dynamically filter by tables, organisms, and agents. View only the information you want to see. Customize for your institution s formulary. Find additional CLSI AST standards and guidelines within the em100 Resource Center. Available at 59

60 Clinical Microbiology Portal Ask questions. Retrieve protocols. Join LISTSERVs. Also from ASM: Clinical Microbiology Procedures Handbook Some Additional Resources 60

61 The following summary slides will not be discussed and are presented for participant s review. 61

62 Summary (1) CLSI develops standards and guidelines for many laboratory procedures including AST. CLSI M100 tables for AST are updated each year; standards describing how to perform disk diffusion tests (M02) and how to perform reference MIC tests (M07) are updated every three years. When using a commercial AST, the manufacturer s recommendations must be followed precisely. 62

63 Summary (2) Selective reporting of antimicrobial agents may encourage prescribing of narrowerspectrum agents and may be appropriate for some facilities. It is dangerous to extrapolate results from one drug to another unless there is an or connecting the two in CLSI tables. Some organism/antimicrobial combinations cannot be reliably tested by the disk diffusion method. 63

64 Summary (3) Some organism/antimicrobial combinations do not work in the patient even though they may appear susceptible in vitro. Some antimicrobial agents are ineffective in treating infections at specific body sites. Some antimicrobial agents are only effective in treating urinary tract infections ( U category in M100). Nonsusceptible results should be confirmed before reporting. 64

65 Summary (4) Interpretive criteria may require revision as new information about organisms or antimicrobial agents becomes available. All patients results must be examined before reporting. Uncommon results should be confirmed or verified. Artificial intelligence or expert software can be extremely helpful to highlight potential problem results. Antimicrobial agent glossaries in M100 list classes and routes of administration for antimicrobial agents. 65

66 66

Antimicrobial Susceptibility Testing: The Basics

Antimicrobial Susceptibility Testing: The Basics Antimicrobial Susceptibility Testing: The Basics Susan E. Sharp, Ph.D., DABMM, FAAM Director, Airport Way Regional Laboratory Director, Regional Microbiology and Molecular Infectious Diseases Laboratories

More information

Antimicrobial Susceptibility Testing: Advanced Course

Antimicrobial Susceptibility Testing: Advanced Course Antimicrobial Susceptibility Testing: Advanced Course Cascade Reporting Cascade Reporting I. Selecting Antimicrobial Agents for Testing and Reporting Selection of the most appropriate antimicrobials to

More information

Educating Clinical and Public Health Laboratories About Antimicrobial Resistance Challenges

Educating Clinical and Public Health Laboratories About Antimicrobial Resistance Challenges Educating Clinical and Public Health Laboratories About Antimicrobial Resistance Challenges Janet Hindler, MCLS MT(ASCP) UCLA Medical Center jhindler@ucla.edu also working as a consultant with the Association

More information

a. 379 laboratories provided quantitative results, e.g (DD method) to 35.4% (MIC method) of all participants; see Table 2.

a. 379 laboratories provided quantitative results, e.g (DD method) to 35.4% (MIC method) of all participants; see Table 2. AND QUANTITATIVE PRECISION (SAMPLE UR-01, 2017) Background and Plan of Analysis Sample UR-01 (2017) was sent to API participants as a simulated urine culture for recognition of a significant pathogen colony

More information

Understanding the Hospital Antibiogram

Understanding the Hospital Antibiogram Understanding the Hospital Antibiogram Sharon Erdman, PharmD Clinical Professor Purdue University College of Pharmacy Infectious Diseases Clinical Pharmacist Eskenazi Health 5 Understanding the Hospital

More information

Help with moving disc diffusion methods from BSAC to EUCAST. Media BSAC EUCAST

Help with moving disc diffusion methods from BSAC to EUCAST. Media BSAC EUCAST Help with moving disc diffusion methods from BSAC to EUCAST This document sets out the main differences between the BSAC and EUCAST disc diffusion methods with specific emphasis on preparation prior to

More information

European Committee on Antimicrobial Susceptibility Testing

European Committee on Antimicrobial Susceptibility Testing European Committee on Antimicrobial Susceptibility Testing Routine and extended internal quality control as recommended by EUCAST Version 5.0, valid from 015-01-09 This document should be cited as "The

More information

جداول میکروارگانیسم های بیماریزای اولویت دار و آنتی بیوتیک های تعیین شده برای آزمایش تعیین حساسیت ضد میکروبی در برنامه مهار مقاومت میکروبی

جداول میکروارگانیسم های بیماریزای اولویت دار و آنتی بیوتیک های تعیین شده برای آزمایش تعیین حساسیت ضد میکروبی در برنامه مهار مقاومت میکروبی جداول میکروارگانیسم های بیماریزای اولویت دار و آنتی بیوتیک های تعیین شده برای آزمایش تعیین حساسیت ضد میکروبی در برنامه مهار مقاومت میکروبی ویرایش دوم بر اساس ed., 2017 CLSI M100 27 th تابستان ۶۹۳۱ تهیه

More information

January 2014 Vol. 34 No. 1

January 2014 Vol. 34 No. 1 January 2014 Vol. 34 No. 1. and Minimum Inhibitory Concentration (MIC) Interpretive Standards for Testing Conditions Medium: diffusion: Mueller-Hinton agar (MHA) Broth dilution: cation-adjusted Mueller-Hinton

More information

EUCAST recommended strains for internal quality control

EUCAST recommended strains for internal quality control EUCAST recommended strains for internal quality control Escherichia coli Pseudomonas aeruginosa Staphylococcus aureus Enterococcus faecalis Streptococcus pneumoniae Haemophilus influenzae ATCC 59 ATCC

More information

January 2014 Vol. 34 No. 1

January 2014 Vol. 34 No. 1 January 2014 Vol. 34 No. 1. and Minimal Inhibitory Concentration (MIC) Interpretive Standards for Testing Conditions Medium: diffusion: Mueller-Hinton agar (MHA) roth dilution: cation-adjusted Mueller-Hinton

More information

European Committee on Antimicrobial Susceptibility Testing

European Committee on Antimicrobial Susceptibility Testing European Committee on Antimicrobial Susceptibility Testing Routine and extended internal quality control for MIC determination and disk diffusion as recommended by EUCAST Version 8.0, valid from 018-01-01

More information

2012 ANTIBIOGRAM. Central Zone Former DTHR Sites. Department of Pathology and Laboratory Medicine

2012 ANTIBIOGRAM. Central Zone Former DTHR Sites. Department of Pathology and Laboratory Medicine 2012 ANTIBIOGRAM Central Zone Former DTHR Sites Department of Pathology and Laboratory Medicine Medically Relevant Pathogens Based on Gram Morphology Gram-negative Bacilli Lactose Fermenters Non-lactose

More information

Routine internal quality control as recommended by EUCAST Version 3.1, valid from

Routine internal quality control as recommended by EUCAST Version 3.1, valid from Routine internal quality control as recommended by EUCAST Version.1, valid from 01-01-01 Escherichia coli Pseudomonas aeruginosa Staphylococcus aureus Enterococcus faecalis Streptococcus pneumoniae Haemophilus

More information

What s new in EUCAST methods?

What s new in EUCAST methods? What s new in EUCAST methods? Derek Brown EUCAST Scientific Secretary Interactive question 1 MIC determination MH-F broth for broth microdilution testing of fastidious microorganisms Gradient MIC tests

More information

Mercy Medical Center Des Moines, Iowa Department of Pathology. Microbiology Department Antibiotic Susceptibility January December 2016

Mercy Medical Center Des Moines, Iowa Department of Pathology. Microbiology Department Antibiotic Susceptibility January December 2016 Mercy Medical Center Des Moines, Iowa Department of Pathology Microbiology Department Antibiotic Susceptibility January December 2016 These statistics are intended solely as a GUIDE to choosing appropriate

More information

Suggestions for appropriate agents to include in routine antimicrobial susceptibility testing

Suggestions for appropriate agents to include in routine antimicrobial susceptibility testing Suggestions for appropriate agents to include in routine antimicrobial susceptibility testing These suggestions are intended to indicate minimum sets of agents to test routinely in a diagnostic laboratory

More information

56 Clinical and Laboratory Standards Institute. All rights reserved.

56 Clinical and Laboratory Standards Institute. All rights reserved. Table 2C 56 Clinical and Laboratory Standards Institute. All rights reserved. Table 2C. Zone Diameter and Minimal Inhibitory Concentration Breakpoints for Testing Conditions Medium: Inoculum: diffusion:

More information

Intrinsic, implied and default resistance

Intrinsic, implied and default resistance Appendix A Intrinsic, implied and default resistance Magiorakos et al. [1] and CLSI [2] are our primary sources of information on intrinsic resistance. Sanford et al. [3] and Gilbert et al. [4] have been

More information

2016 Antibiotic Susceptibility Report

2016 Antibiotic Susceptibility Report Fairview Northland Medical Center and Elk River, Milaca, Princeton and Zimmerman Clinics 2016 Antibiotic Susceptibility Report GRAM-NEGATIVE ORGANISMS 2016 Gram-Negative Non-Urine The number of isolates

More information

THE NAC CHALLENGE PANEL OF ISOLATES FOR VERIFICATION OF ANTIBIOTIC SUSCEPTIBILITY TESTING METHODS

THE NAC CHALLENGE PANEL OF ISOLATES FOR VERIFICATION OF ANTIBIOTIC SUSCEPTIBILITY TESTING METHODS THE NAC CHALLENGE PANEL OF ISOLATES FOR VERIFICATION OF ANTIBIOTIC SUSCEPTIBILITY TESTING METHODS Stefanie Desmet University Hospitals Leuven Laboratory medicine microbiology stefanie.desmet@uzleuven.be

More information

2015 Antibiotic Susceptibility Report

2015 Antibiotic Susceptibility Report Citrobacter freundii Enterobacter aerogenes Enterobacter cloacae Escherichia coli Haemophilus influenzenza Klebsiella oxytoca Klebsiella pneumoniae Proteus mirabilis Pseudomonas aeruginosa Serratia marcescens

More information

Background and Plan of Analysis

Background and Plan of Analysis ENTEROCOCCI Background and Plan of Analysis UR-11 (2017) was sent to API participants as a simulated urine culture for recognition of a significant pathogen colony count, to perform the identification

More information

Concise Antibiogram Toolkit Background

Concise Antibiogram Toolkit Background Background This toolkit is designed to guide nursing homes in creating their own antibiograms, an important tool for guiding empiric antimicrobial therapy. Information about antibiograms and instructions

More information

Childrens Hospital Antibiogram for 2012 (Based on data from 2011)

Childrens Hospital Antibiogram for 2012 (Based on data from 2011) Childrens Hospital Antibiogram for 2012 (Based on data from 2011) Prepared by: Department of Clinical Microbiology, Health Sciences Centre For further information contact: Andrew Walkty, MD, FRCPC Medical

More information

Leveraging the Lab and Microbiology Department to Optimize Stewardship

Leveraging the Lab and Microbiology Department to Optimize Stewardship Leveraging the Lab and Microbiology Department to Optimize Stewardship Presented by: Andrew Martinez MLS(ASCP), MT(AMT), MBA Alaska Native Medical Center Microbiology Supervisor Maniilaq Health Center

More information

SAMPLE VET08. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals.

SAMPLE VET08. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals. VET08 4th Edition Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals This document includes updated tables for the Clinical and Laboratory

More information

There are two international organisations that set up guidelines and interpretive breakpoints for bacteriology and susceptibility

There are two international organisations that set up guidelines and interpretive breakpoints for bacteriology and susceptibility ANTIMICROBIAL SUSCEPTIBILITY TESTING ON MILK SAMPLES Method and guidelines There are two international organisations that set up guidelines and interpretive breakpoints for bacteriology and susceptibility

More information

Compliance of manufacturers of AST materials and devices with EUCAST guidelines

Compliance of manufacturers of AST materials and devices with EUCAST guidelines Compliance of manufacturers of AST materials and devices with EUCAST guidelines Data are based on questionnaires to manufacturers of materials and devices for antimicrobial susceptibility testing. The

More information

Performance Information. Vet use only

Performance Information. Vet use only Performance Information Vet use only Performance of plates read manually was measured in three sites. Each centre tested Enterobacteriaceae, streptococci, staphylococci and pseudomonas-like organisms.

More information

Compliance of manufacturers of AST materials and devices with EUCAST guidelines

Compliance of manufacturers of AST materials and devices with EUCAST guidelines Compliance of manufacturers of AST materials and devices with EUCAST guidelines Data are based on questionnaires to manufacturers of materials and devices for antimicrobial susceptibility testing. The

More information

Defining Resistance and Susceptibility: What S, I, and R Mean to You

Defining Resistance and Susceptibility: What S, I, and R Mean to You Defining Resistance and Susceptibility: What S, I, and R Mean to You Michael D. Apley, DVM, PhD, DACVCP Department of Clinical Sciences College of Veterinary Medicine Kansas State University Susceptible

More information

Antimicrobial Stewardship Strategy: Antibiograms

Antimicrobial Stewardship Strategy: Antibiograms Antimicrobial Stewardship Strategy: Antibiograms A summary of the cumulative susceptibility of bacterial isolates to formulary antibiotics in a given institution or region. Its main functions are to guide

More information

Practical approach to Antimicrobial susceptibility testing (AST) and quality control

Practical approach to Antimicrobial susceptibility testing (AST) and quality control Practical approach to Antimicrobial susceptibility testing (AST) and quality control A/Professor John Ferguson, Microbiologist & Infectious Diseases Physician, Pathology North, University of Newcastle,

More information

Streptococcus pneumoniae. Oxacillin 1 µg as screen for beta-lactam resistance

Streptococcus pneumoniae. Oxacillin 1 µg as screen for beta-lactam resistance Streptococcus pneumoniae Oxacillin µg as screen for beta-lactam resistance Version 6. June Streptococcus pneumoniae and zone diameter correlates The following histograms present inhibition zone diameter

More information

CONTAGIOUS COMMENTS Department of Epidemiology

CONTAGIOUS COMMENTS Department of Epidemiology VOLUME XXIX NUMBER 3 November 2014 CONTAGIOUS COMMENTS Department of Epidemiology Bugs and Drugs Elaine Dowell SM MLS (ASCP), Marti Roe SM MLS (ASCP), Sarah Parker MD, Jason Child PharmD, and Samuel R.

More information

2017 Antibiogram. Central Zone. Alberta Health Services. including. Red Deer Regional Hospital. St. Mary s Hospital, Camrose

2017 Antibiogram. Central Zone. Alberta Health Services. including. Red Deer Regional Hospital. St. Mary s Hospital, Camrose 2017 Antibiogram Central Zone Alberta Health Services including Red Deer Regional Hospital St. Mary s Hospital, Camrose Introduction This antibiogram is a cumulative report of the antimicrobial susceptibility

More information

Surveillance for Antimicrobial Resistance and Preparation of an Enhanced Antibiogram at the Local Level. janet hindler

Surveillance for Antimicrobial Resistance and Preparation of an Enhanced Antibiogram at the Local Level. janet hindler Surveillance for Antimicrobial Resistance and Preparation of an Enhanced Antibiogram at the Local Level janet hindler At the conclusion of this talk, you will be able to Describe CLSI M39-A3 recommendations

More information

Version 1.01 (01/10/2016)

Version 1.01 (01/10/2016) CHN58: ANTIMICROBIAL SUSCEPTIBILITY TESTING (CLSI) 1.0 PURPOSE / INTRODUCTION: 1.1 Introduction Antimicrobial susceptibility tests are performed in order to determine whether a pathogen is likely to be

More information

2 0 hr. 2 hr. 4 hr. 8 hr. 10 hr. 12 hr.14 hr. 16 hr. 18 hr. 20 hr. 22 hr. 24 hr. (time)

2 0 hr. 2 hr. 4 hr. 8 hr. 10 hr. 12 hr.14 hr. 16 hr. 18 hr. 20 hr. 22 hr. 24 hr. (time) Key words I μ μ μ μ μ μ μ μ μ μ μ μ μ μ II Fig. 1. Microdilution plate. The dilution step of the antimicrobial agent is prepared in the -well microplate. Serial twofold dilution were prepared according

More information

2016 Antibiogram. Central Zone. Alberta Health Services. including. Red Deer Regional Hospital. St. Mary s Hospital, Camrose

2016 Antibiogram. Central Zone. Alberta Health Services. including. Red Deer Regional Hospital. St. Mary s Hospital, Camrose 2016 Antibiogram Central Zone Alberta Health Services including Red Deer Regional Hospital St. Mary s Hospital, Camrose Introduction This antibiogram is a cumulative report of the antimicrobial susceptibility

More information

UNDERSTANDING YOUR DATA: THE ANTIBIOGRAM

UNDERSTANDING YOUR DATA: THE ANTIBIOGRAM UNDERSTANDING YOUR DATA: THE ANTIBIOGRAM April Abbott, PhD, D(ABMM) Deaconess Health System Evansville, IN April.Abbott@Deaconess.com Special thanks to Dr. Shelley Miller for UCLA data WHAT WE WILL COVER

More information

EDUCATIONAL COMMENTARY - Methicillin-Resistant Staphylococcus aureus: An Update

EDUCATIONAL COMMENTARY - Methicillin-Resistant Staphylococcus aureus: An Update EDUCATIONAL COMMENTARY - Methicillin-Resistant Staphylococcus aureus: An Update Educational commentary is provided through our affiliation with the American Society for Clinical Pathology (ASCP). To obtain

More information

ESCMID Online Lecture Library. by author

ESCMID Online Lecture Library. by author Quality Assurance of antimicrobial susceptibility testing Derek Brown EUCAST Scientific Secretary ESCMID Postgraduate Education Course, Linz, 17 September 2014 Quality Assurance The total process by which

More information

Detecting / Reporting Resistance in Nonfastidious GNR Part #2. Janet A. Hindler, MCLS MT(ASCP)

Detecting / Reporting Resistance in Nonfastidious GNR Part #2. Janet A. Hindler, MCLS MT(ASCP) Detecting / Reporting Resistance in Nonfastidious GNR Part #2 Janet A. Hindler, MCLS MT(ASCP) Methods Described in CLSI M100-S21 for Testing non-enterobacteriaceae Organism Disk Diffusion MIC P. aeruginosa

More information

SAMPLE. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals

SAMPLE. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals VET01 5th Edition Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals This standard covers the current recommended methods for disk diffusion

More information

2015 Antibiogram. Red Deer Regional Hospital. Central Zone. Alberta Health Services

2015 Antibiogram. Red Deer Regional Hospital. Central Zone. Alberta Health Services 2015 Antibiogram Red Deer Regional Hospital Central Zone Alberta Health Services Introduction. This antibiogram is a cumulative report of the antimicrobial susceptibility rates of common microbial pathogens

More information

CONTAGIOUS COMMENTS Department of Epidemiology

CONTAGIOUS COMMENTS Department of Epidemiology VOLUME XXIII NUMBER 1 July 2008 CONTAGIOUS COMMENTS Department of Epidemiology Bugs and Drugs Elaine Dowell, SM (ASCP), Marti Roe SM (ASCP), Ann-Christine Nyquist MD, MSPH Are the bugs winning? The 2007

More information

Antimicrobial susceptibility

Antimicrobial susceptibility Antimicrobial susceptibility PATTERNS Microbiology Department Canterbury ealth Laboratories and Clinical Pharmacology Department Canterbury District ealth Board March 2011 Contents Preface... Page 1 ANTIMICROBIAL

More information

GENERAL NOTES: 2016 site of infection type of organism location of the patient

GENERAL NOTES: 2016 site of infection type of organism location of the patient GENERAL NOTES: This is a summary of the antibiotic sensitivity profile of clinical isolates recovered at AIIMS Bhopal Hospital during the year 2016. However, for organisms in which < 30 isolates were recovered

More information

This document is protected by international copyright laws.

This document is protected by international copyright laws. Table 2C Table 2C. and s for Product Name: Infobase 2010 - Release Date: February 2010 60 Clinical and Laboratory Standards Institute. All rights reserved. Testing Conditions Medium: diffusion: MHA Broth

More information

Antibiotic. Antibiotic Classes, Spectrum of Activity & Antibiotic Reporting

Antibiotic. Antibiotic Classes, Spectrum of Activity & Antibiotic Reporting Antibiotic Antibiotic Classes, Spectrum of Activity & Antibiotic Reporting Any substance of natural, synthetic or semisynthetic origin which at low concentrations kills or inhibits the growth of bacteria

More information

RCH antibiotic susceptibility data

RCH antibiotic susceptibility data RCH antibiotic susceptibility data The following represent RCH antibiotic susceptibility data from 2008. This data is used to inform antibiotic guidelines used at RCH. The data includes all microbiological

More information

QUICK REFERENCE. Pseudomonas aeruginosa. (Pseudomonas sp. Xantomonas maltophilia, Acinetobacter sp. & Flavomonas sp.)

QUICK REFERENCE. Pseudomonas aeruginosa. (Pseudomonas sp. Xantomonas maltophilia, Acinetobacter sp. & Flavomonas sp.) Pseudomonas aeruginosa (Pseudomonas sp. Xantomonas maltophilia, Acinetobacter sp. & Flavomonas sp.) Description: Greenish gray colonies with some beta-hemolysis around each colony on blood agar (BAP),

More information

MICRONAUT MICRONAUT-S Detection of Resistance Mechanisms. Innovation with Integrity BMD MIC

MICRONAUT MICRONAUT-S Detection of Resistance Mechanisms. Innovation with Integrity BMD MIC MICRONAUT Detection of Resistance Mechanisms Innovation with Integrity BMD MIC Automated and Customized Susceptibility Testing For detection of resistance mechanisms and specific resistances of clinical

More information

Antimicrobial Susceptibility Patterns

Antimicrobial Susceptibility Patterns Antimicrobial Susceptibility Patterns KNH SURGERY Department Masika M.M. Department of Medical Microbiology, UoN Medicines & Therapeutics Committee, KNH Outline Methodology Overall KNH data Surgery department

More information

UNDERSTANDING THE ANTIBIOGRAM

UNDERSTANDING THE ANTIBIOGRAM UNDERSTANDING THE ANTIBIOGRAM April Abbott, PhD, D(ABMM) Deaconess Health System Indiana University School of Medicine - Evansville Evansville, IN April.Abbott@Deaconess.com WHAT WE WILL COVER Describe

More information

EARS Net Report, Quarter

EARS Net Report, Quarter EARS Net Report, Quarter 4 213 March 214 Key Points for 213* Escherichia coli: The proportion of patients with invasive infections caused by E. coli producing extended spectrum β lactamases (ESBLs) increased

More information

National Clinical Guideline Centre Pneumonia Diagnosis and management of community- and hospital-acquired pneumonia in adults

National Clinical Guideline Centre Pneumonia Diagnosis and management of community- and hospital-acquired pneumonia in adults National Clinical Guideline Centre Antibiotic classifications Pneumonia Diagnosis and management of community- and hospital-acquired pneumonia in adults Clinical guideline 191 Appendix N 3 December 2014

More information

Interpreting Microbiology reports for better Clinical Decisions Interpreting Antibiogrammes

Interpreting Microbiology reports for better Clinical Decisions Interpreting Antibiogrammes Interpreting Microbiology reports for better Clinical Decisions Interpreting Antibiogrammes Prof C. Wattal Hon. Sr. Consultant & Chairman Dept. of Clinical Microbiology Sir Ganga Ram Hospital New Delhi

More information

Lab Exercise: Antibiotics- Evaluation using Kirby Bauer method.

Lab Exercise: Antibiotics- Evaluation using Kirby Bauer method. Lab Exercise: Antibiotics- Evaluation using Kirby Bauer method. OBJECTIVES 1. Compare the antimicrobial capabilities of different antibiotics. 2. Compare effectiveness of with different types of bacteria.

More information

ESBL Producers An Increasing Problem: An Overview Of An Underrated Threat

ESBL Producers An Increasing Problem: An Overview Of An Underrated Threat ESBL Producers An Increasing Problem: An Overview Of An Underrated Threat Hicham Ezzat Professor of Microbiology and Immunology Cairo University Introduction 1 Since the 1980s there have been dramatic

More information

ENTEROCOCCI. April Abbott Deaconess Health System Evansville, IN

ENTEROCOCCI. April Abbott Deaconess Health System Evansville, IN ENTEROCOCCI April Abbott Deaconess Health System Evansville, IN OBJECTIVES Discuss basic antimicrobial susceptibility principles and resistance mechanisms for Enterococcus Describe issues surrounding AST

More information

Aberdeen Hospital. Antibiotic Susceptibility Patterns For Commonly Isolated Organisms For 2015

Aberdeen Hospital. Antibiotic Susceptibility Patterns For Commonly Isolated Organisms For 2015 Aberdeen Hospital Antibiotic Susceptibility Patterns For Commonly Isolated s For 2015 Services Laboratory Microbiology Department Aberdeen Hospital Nova Scotia Health Authority 835 East River Road New

More information

INFECTIOUS DISEASES DIAGNOSTIC LABORATORY NEWSLETTER

INFECTIOUS DISEASES DIAGNOSTIC LABORATORY NEWSLETTER INFECTIOUS DISEASES DIAGNOSTIC LABORATORY NEWSLETTER University of Minnesota Health University of Minnesota Medical Center University of Minnesota Masonic Children s Hospital May 2017 Printed herein are

More information

Evaluation of the BIOGRAM Antimicrobial Susceptibility Test System

Evaluation of the BIOGRAM Antimicrobial Susceptibility Test System JOURNAL OF CLINICAL MICROBIOLOGY, Nov. 1985, p. 793-798 0095-1137/85/110793-06$02.00/0 Copyright 1985, American Society for Microbiology Vol. 22, No. 5 Evaluation of the BIOGRAM Antimicrobial Susceptibility

More information

Int.J.Curr.Microbiol.App.Sci (2017) 6(11):

Int.J.Curr.Microbiol.App.Sci (2017) 6(11): International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 11 (2017) pp. 2293-2299 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.611.272

More information

Helen Heffernan and Rosemary Woodhouse Antibiotic Reference Laboratory

Helen Heffernan and Rosemary Woodhouse Antibiotic Reference Laboratory METHODS USED IN NEW ZEALAND DIAGNOSTIC LABORATORIES TO IDENTIFY AND REPORT EXTENDED-SPECTRUM β-lactamase- PRODUCING ENTEROBACTERIACEAE by Helen Heffernan and Rosemary Woodhouse Antibiotic Reference Laboratory

More information

C&W Three-Year Cumulative Antibiogram January 2013 December 2015

C&W Three-Year Cumulative Antibiogram January 2013 December 2015 C&W Three-Year Cumulative Antibiogram January 213 December 215 Division of Microbiology, Virology & Infection Control Department of Pathology & Laboratory Medicine Contents Comments and Limitations...

More information

21 st Expert Committee on Selection and Use of Essential Medicines Peer Review Report Antibiotics Review

21 st Expert Committee on Selection and Use of Essential Medicines Peer Review Report Antibiotics Review (1) Have all important studies/evidence of which you are aware been included in the application? Yes No Please provide brief comments on any relevant studies that have not been included: (2) For each of

More information

Available online at ISSN No:

Available online at  ISSN No: Available online at www.ijmrhs.com ISSN No: 2319-5886 International Journal of Medical Research & Health Sciences, 2017, 6(4): 36-42 Comparative Evaluation of In-Vitro Doripenem Susceptibility with Other

More information

The Nuts and Bolts of Antibiograms in Long-Term Care Facilities

The Nuts and Bolts of Antibiograms in Long-Term Care Facilities The Nuts and Bolts of Antibiograms in Long-Term Care Facilities J. Kristie Johnson, Ph.D., D(ABMM) Professor, Department of Pathology University of Maryland School of Medicine Director, Microbiology Laboratories

More information

Evaluation of a computerized antimicrobial susceptibility system with bacteria isolated from animals

Evaluation of a computerized antimicrobial susceptibility system with bacteria isolated from animals J Vet Diagn Invest :164 168 (1998) Evaluation of a computerized antimicrobial susceptibility system with bacteria isolated from animals Susannah K. Hubert, Phouc Dinh Nguyen, Robert D. Walker Abstract.

More information

Prevalence of Metallo-Beta-Lactamase Producing Pseudomonas aeruginosa and its antibiogram in a tertiary care centre

Prevalence of Metallo-Beta-Lactamase Producing Pseudomonas aeruginosa and its antibiogram in a tertiary care centre International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 4 Number 9 (2015) pp. 952-956 http://www.ijcmas.com Original Research Article Prevalence of Metallo-Beta-Lactamase

More information

2010 ANTIBIOGRAM. University of Alberta Hospital and the Stollery Children s Hospital

2010 ANTIBIOGRAM. University of Alberta Hospital and the Stollery Children s Hospital 2010 ANTIBIOGRAM University of Alberta Hospital and the Stollery Children s Hospital Medical Microbiology Department of Laboratory Medicine and Pathology Table of Contents Page Introduction..... 2 Antibiogram

More information

2015 Antimicrobial Susceptibility Report

2015 Antimicrobial Susceptibility Report Gram negative Sepsis Outcome Programme (GNSOP) 2015 Antimicrobial Susceptibility Report Prepared by A/Professor Thomas Gottlieb Concord Hospital Sydney Jan Bell The University of Adelaide Adelaide On behalf

More information

BSAC standardized disc susceptibility testing method (version 8)

BSAC standardized disc susceptibility testing method (version 8) Journal of Antimicrobial Chemotherapy (2009) 64, 454 489 doi:10.1093/jac/dkp244 Advance Access publication 8 July 2009 BSAC standardized disc susceptibility testing method (version 8) J. M. Andrews* for

More information

SMART WORKFLOW SOLUTIONS Introducing DxM MicroScan WalkAway System* ...

SMART WORKFLOW SOLUTIONS Introducing DxM MicroScan WalkAway System* ... SMART WORKFLOW SOLUTIONS Introducing DxM MicroScan WalkAway System* The next-generation MicroScan WalkAway System combines proven technology and reliability with enhanced ease-of-use features to streamline

More information

BactiReg3 Event Notes Module Page(s) 4-9 (TUL) Page 1 of 21

BactiReg3 Event Notes Module Page(s) 4-9 (TUL) Page 1 of 21 www.wslhpt.org 2601 Agriculture Drive Madison, WI 53718 (800) 462-5261 (608) 265-1111 2015-BactiR Reg3 Shipment Date: September 14, 2015 Questions or comments should be directed to Amanda Weiss at 800-462-5261

More information

Appropriate antimicrobial therapy in HAP: What does this mean?

Appropriate antimicrobial therapy in HAP: What does this mean? Appropriate antimicrobial therapy in HAP: What does this mean? Jaehee Lee, M.D. Kyungpook National University Hospital, Korea KNUH since 1907 Presentation outline Empiric antimicrobial choice: right spectrum,

More information

CONTAGIOUS COMMENTS Department of Epidemiology

CONTAGIOUS COMMENTS Department of Epidemiology VOLUME XXXII NUMBER 6 September 2017 CONTAGIOUS COMMENTS Department of Epidemiology Bugs and Drugs Elaine Dowell SM MLS (ASCP), Stacey Hamilton MT SM (ASCP), Samuel Dominguez MD PhD, Sarah Parker MD, and

More information

GeNei TM. Antibiotic Sensitivity. Teaching Kit Manual KT Revision No.: Bangalore Genei, 2007 Bangalore Genei, 2007

GeNei TM. Antibiotic Sensitivity. Teaching Kit Manual KT Revision No.: Bangalore Genei, 2007 Bangalore Genei, 2007 GeNei Bacterial Antibiotic Sensitivity Teaching Kit Manual Cat No. New Cat No. KT68 106333 Revision No.: 00180705 CONTENTS Page No. Objective 3 Principle 3 Kit Description 4 Materials Provided 5 Procedure

More information

The Very Latest from the CLSI AST Subcommittee.

The Very Latest from the CLSI AST Subcommittee. 2 0 1 4 The Very Latest from the CLSI AST Subcommittee. Susan E. Sharp, Ph.D., DABMM, FAAM Director - Regional Laboratory Director - Regional Microbiology/Molecular Infectious Diseases Diagnostics Laboratory

More information

2009 ANTIBIOGRAM. University of Alberta Hospital and the Stollery Childrens Hospital

2009 ANTIBIOGRAM. University of Alberta Hospital and the Stollery Childrens Hospital 2009 ANTIBIOGRAM University of Alberta Hospital and the Stollery Childrens Hospital Division of Medical Microbiology Department of Laboratory Medicine and Pathology 2 Table of Contents Page Introduction.....

More information

Update on the CLSI Standards for Antimicrobial Susceptibility Testing: What s New with the Gram Positive Organisms?

Update on the CLSI Standards for Antimicrobial Susceptibility Testing: What s New with the Gram Positive Organisms? 2 0 1 3 CASES (with answers!) Update on the CLSI Standards for Antimicrobial Susceptibility Testing: What s New with the Gram Positive Organisms? Susan E. Sharp, Ph.D., DABMM, FAAM Director - Regional

More information

Table 1. Commonly encountered or important organisms and their usual antimicrobial susceptibilities.

Table 1. Commonly encountered or important organisms and their usual antimicrobial susceptibilities. Table 1. Commonly encountered or important organisms and their usual antimicrobial susceptibilities. Gram-positive cocci: Staphylococcus aureus: *Resistance to penicillin is almost universal. Resistance

More information

Prevalence of Extended Spectrum Beta- Lactamase Producers among Various Clinical Samples in a Tertiary Care Hospital: Kurnool District, India

Prevalence of Extended Spectrum Beta- Lactamase Producers among Various Clinical Samples in a Tertiary Care Hospital: Kurnool District, India International Journal of Current Microbiology and Applied Sciences ISSN: 319-77 Volume Number (17) pp. 57-3 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/1.5/ijcmas.17..31

More information

microbiology testing services

microbiology testing services microbiology testing services You already know Spectra Laboratories for a wide array of dialysis-related testing services. Now get to know us for your microbiology needs. As the leading provider of renal-specific

More information

Florida Health Care Association District 2 January 13, 2015 A.C. Burke, MA, CIC

Florida Health Care Association District 2 January 13, 2015 A.C. Burke, MA, CIC Florida Health Care Association District 2 January 13, 2015 A.C. Burke, MA, CIC 11/20/2014 1 To describe carbapenem-resistant Enterobacteriaceae. To identify laboratory detection standards for carbapenem-resistant

More information

Comparison of antibiotic susceptibility results obtained with Adatab* and disc methods

Comparison of antibiotic susceptibility results obtained with Adatab* and disc methods J Clin Pathol 1984;37:159-165 Comparison of antibiotic susceptibility results obtained with Adatab* and disc methods JJS SNELL, MVS DANVERS, PS GARDNER From the Division of Microbiological Reagents and

More information

EXTENDED-SPECTRUM BETA-LACTAMASE (ESBL) TESTING

EXTENDED-SPECTRUM BETA-LACTAMASE (ESBL) TESTING EXTENDED-SPECTRUM BETA-LACTAMASE (ESBL) TESTING CHN61: EXTENDED-SPECTRUM BETA-LACTAMASE (ESBL) TESTING 1.1 Introduction A common mechanism of bacterial resistance to beta-lactam antibiotics is the production

More information

EUCAST-and CLSI potency NEO-SENSITABS

EUCAST-and CLSI potency NEO-SENSITABS EUCASTand CLSI potency NEOSENSITABS Neo Sensitabs Page 1 / 6 Document: 6.2.0 Fastidious organisms EUCAST Interpretation zones and MIC breakpoints according to recommendations by the "Comité de l'antibiogramme

More information

Antimicrobial Resistance Surveillance from sentinel public hospitals, South Africa, 2013

Antimicrobial Resistance Surveillance from sentinel public hospitals, South Africa, 2013 Antimicrobial Resistance Surveillance from sentinel public s, South Africa, 213 Authors: Olga Perovic 1,2, Melony Fortuin-de Smidt 1, and Verushka Chetty 1 1 National Institute for Communicable Diseases

More information

CAP Laboratory Improvement Programs. Performance Accuracy of Antibacterial and Antifungal Susceptibility Test Methods

CAP Laboratory Improvement Programs. Performance Accuracy of Antibacterial and Antifungal Susceptibility Test Methods CAP Laboratory Improvement Programs Performance Accuracy of Antibacterial and Antifungal usceptibility Test Methods Report From the College of American Pathologists Microbiology urveys Program (001 003)

More information

Antibiotic Usage Guidelines in Hospital

Antibiotic Usage Guidelines in Hospital SUPPLEMENT TO JAPI december VOL. 58 51 Antibiotic Usage Guidelines in Hospital Camilla Rodrigues * Use of surveillance data information of Hospital antibiotic policy guidelines from Hinduja Hospital. The

More information

Michael Hombach*, Guido V. Bloemberg and Erik C. Böttger

Michael Hombach*, Guido V. Bloemberg and Erik C. Böttger J Antimicrob Chemother 2012; 67: 622 632 doi:10.1093/jac/dkr524 Advance Access publication 13 December 2011 Effects of clinical breakpoint changes in CLSI guidelines 2010/2011 and EUCAST guidelines 2011

More information

Quality assurance of antimicrobial susceptibility testing

Quality assurance of antimicrobial susceptibility testing Quality assurance of antimicrobial susceptibility testing Derek Brown Routine quality control Repeated testing of controls in parallel with tests to ensure that the test system is performing reproducibly

More information

The β- Lactam Antibiotics. Munir Gharaibeh MD, PhD, MHPE School of Medicine, The University of Jordan November 2018

The β- Lactam Antibiotics. Munir Gharaibeh MD, PhD, MHPE School of Medicine, The University of Jordan November 2018 The β- Lactam Antibiotics Munir Gharaibeh MD, PhD, MHPE School of Medicine, The University of Jordan November 2018 Penicillins. Cephalosporins. Carbapenems. Monobactams. The β- Lactam Antibiotics 2 3 How

More information

Short Report. R Boot. Keywords: Bacteria, antimicrobial susceptibility testing, quality, diagnostic laboratories, proficiency testing

Short Report. R Boot. Keywords: Bacteria, antimicrobial susceptibility testing, quality, diagnostic laboratories, proficiency testing Short Report Frequent major errors in antimicrobial susceptibility testing of bacterial strains distributed under the Deutsches Krebsforschungszentrum Quality Assurance Program R Boot Former Section of

More information

Principles and Practice of Antimicrobial Susceptibility Testing. Microbiology Technical Workshop 25 th September 2013

Principles and Practice of Antimicrobial Susceptibility Testing. Microbiology Technical Workshop 25 th September 2013 Principles and Practice of Antimicrobial Susceptibility Testing Microbiology Technical Workshop 25 th September 2013 Scope History Why Perform Antimicrobial Susceptibility Testing? How to Perform an Antimicrobial

More information