General Policies... i. Necropsy Histopathology Immunohistochemistry Bacteriology Bacteriology Serology Toxicology...

Transcription

1 TABLE OF CONTENTS General Policies... i Necropsy... 1 Clinical Pathology/Cytology... 1 Histopathology... 2 Immunohistochemistry... 3 Bacteriology... 4 Bacteriology Molecular Diagnostics (PCR)... 6 Bacteriology Parasitology... 8 Bacteriology Serology... 9 Toxicology Virology Virology Molecular Diagnostic (PCR) Virology Serology Media and Supplies, Misc. Information Animal Disease Phone/Fax Numbers University of Nebraska Veterinary Diagnostic Fee Schedule

2 GENERAL POLICIES We very much appreciate your patronage. To promote clear communication, efficient and prompt service, and uniform policies applied by our staff, the following business operating guidelines were established. 1. Accessions An accession represents a laboratory submission from a single clinical problem (disease syndrome) from one herd. An accession may consist of single or multiple animals or specimens from the same production unit, same species, and same age group, with one clinical problem. For multiple animals to be considered a single accession the lab must retain the right to select samples for additional testing and pool testing at the discretion of the case coordinator to mitigate costs. Pooling is only done when no loss in diagnostic sensitivity is anticipated. -Submissions consisting of multiple animals with different clinical problems will be handled as separate accessions and will be charged individually with individual case breakpoints. -Multiple specimens submitted for specified testing (not marked discretion of the laboratory) in which identification of all individual samples is retained may be billed with one accession fee but the break point may not apply. (eg. 12 Serpulina cultures) - Submitting of samples implies agreement to policies of the Laboratory and transfers ownership of samples and property intellectual or otherwise derived from samples unless ownership is retained via written agreement. - Agreement to allow subcontracting of tests not currently performed is implied by submission to our facility. - Most testing will be performed at the UNL campus in compliance with our Quality Assurance documents. Subcontracted tests will be noted and the test site will be specified in the final report. Subcontracted tests will be contracted to labs accredited to equal or higher standards or a quality audit will be performed. 2. Payment The person requesting laboratory services is responsible for payment. The submitting veterinarian will be billed for services forwarded on behalf of clients. Veterinarians should not request that the Diagnostic Center bill owners directly for services. Owners requesting service without a veterinarian are required to pay at the time of sample delivery. Laboratory charges are billed each month and payment is due upon receipt. Laboratory services will be suspended if an account is more than 60 days past due. Effective as of April 1, 2004, a returned check service fee of $30.00 will be charged for each returned check. The University/Veterinary Diagnostic Center is not set up to accept payments by credit cards. Payment must be made by check or by cash only. 3. Breakpoint Fees A graduated fee schedule has been established to maintain fees to individual animal owners at the lowest rate possible. This fee schedule includes a "fee breakpoint" beyond which the fees are discounted 90%. The fee breakpoint is $ on accessions. Included in the breakpoint fee are the charges for the accession fee, necropsy, histopathology, immunohistochemistry, bacteriology, and virology. -Laboratory fees exceeding the breakpoints will be billed at the rate of $ plus 10% of the remaining laboratory fees. For example, a case which includes necropsy and laboratory fees that total $ would be charged $ plus $9.00 (10% of the remaining $90 in fees) for a total of $ Break point fees exclude serology, toxicology charges and disposal fee. University of Nebraska Veterinary Diagnostic Fee Schedule

3 Charges for serology and toxicology, specialized molecular tests and large animal disposal will be billed in addition to the total of the fees for laboratory tests conducted. Likewise, professional service fees for research or litigation work and photo documentation will not be included under the fee breakpoint. -The above case with paired serology totaling $40 would be billed at $140 ( ) Industrial manufacturing firms are not regarded as individual animal owners for purposes of laboratory services. The graduated "fee breakpoint" policy does not apply to accessions submitted by such firms, and they will be charged the full laboratory fee costs. - If multiple methods are requested for identification of the same agent, the Lab may charge for additional test in excess of the breakpoint. 4. Accession and Shipping Fees A $7.00 accession fee will be charged on most accessions to help defray cost of tracking, reporting, billing, and professional services. A $12.50 fee will be levied for packaging and forwarding specimens to reference laboratories. Specimens requiring express mail or special handling will be charged all related shipping costs. Shipping bacterial isolates usually results in extra charges and does require return of shipping. On return, UN certified s will be billed at cost. 5. Disposal The labs now pay a rendering fee associated with eliminating animal remains. All sheep must be incinerated. A fee of $10.00 for sheep weighing less than 50 pounds and $25.00 for sheep greater than 50 pounds will be added to the charges. Disposal fees are subject to change. Current fees for other species are on the fee schedule. 6. Biopsy Fees Fees for biopsies, which are charged at $20.00 per site, include multiple biopsies from one site or multiple sites for a generalized dermatologic condition. Up to three special stains may be included at the discretion of the pathologist. Charges incurred due to referral to another lab for second opinions may be charged if shipped at your request. Second opinion review of sections in-house will be done free of charge. For multiple tissues (necropsy in a bottle) or small animal necropsy, the lab can provide an estimate of charges to assist your billing cash-only clients. Call for an estimate if needed 7. Unanticipated Fees Significant additional or unanticipated testing will be performed only after consultation with and approval by the person who requested laboratory services. Additional testing will be charged at the published rate. University of Nebraska Veterinary Diagnostic Fee Schedule

4 Effective July 1, 2008 NECROPSY: Necropsy only (3 animals, multiple birds) no additional testing will be conducted Necropsy, complete (with basic tests) $40.00 Whole or partial cadaver See Breakpoint Fees Cosmetic necropsy $50.00 $20.00 additional for examination of brain Disposal Fees: Cows & Pigs > (greater than) 120 lbs. $25.00 Horses $ Foals $25.00 Sheep > (greater than) 50 lbs. $25.00 Whole or partial cadaver Whole or partial cadaver Ice if sent by mail Ice if sent by mail M F Do not freeze specimen(s) M F Brain may not be examined; notify Diagnostic Center before necropsy if the brain is to be examined. CLINICAL PATHOLOGY/CYTOLOGY: Exfoliative Cytology (Impression smears, fine needle aspirates) Fluid Examination (includes bacteriologic culture, sensitivity, cytology & total protein $20.00/sample Slides; needle aspirates $27.50/sample M F M F Do not freeze specimen(s); M F 24 hrs. Be sure that slides are sent in a breakproof. University of Nebraska Veterinary Diagnostic Fee Schedule Page 1

5 CLINICAL PATHOLOGY/CYTOLOGY: (cont d.) Serum Ig (electrophoresis) $10.00/sample M F Urinanalysis I (includes $20.00/sample Urine M F bacteriologic culture, Multistick exam, specific gravity and sediment exam) Urinanalysis II (Sediment $12.50/sample Urine M F exam and Cytology only) HISTOPATHOLOGY: Biopsy $20.00/site Formalin fixed tissues Postmortem Species (multiple tissues) $10.00/slide Brain $20.00 per 5 slides Mare Uterine Biopsy (Special stains included at no extra charge) Formalin fixed tissues Formalin fixed tissues $20.00 Formalin fixed tissues Research pathology Formalin fixed tissues M F Be sure that lids are secure and that any bags are leakproof; put paperwork in a separate zip lock bag M F Be sure that bags/s are leakproof; put paperwork in a separate ziplock bag M F M F Call for rate. Rate will be based on marginal cost of testing performed. University of Nebraska Veterinary Diagnostic Fee Schedule Page 2

6 IMMUNOHISTOCHEMISTRY: Bovine: BCV, BVD,BRSV,IBR,Neospora,Ty pe II Collagen Porcine: Brachyspira, Circo, Lepto, parvo, PRRS,SIV,TGE Canine: IgG,Cytokeratins,Vimenten, Desmin,Endothelium,S 100,Melan A Any Species: Chlamydia,Corona,Lepto, Rotavirus group A, Synaptophysin,GFAP $12.00/antigen $12.00/antigen $12.00/antigen $12.00antigen Formalinfixed tissues Formalinfixed tissues Formalinfixed tissues Formalinfixed tissues Skin BVD/IHC, multiple samples run (up to 6 per block) $20.00 for each block used Skin BVD/IHC, 1 sample $12.00 Formalinfixed tissues Formalinfixed tissues M F M F M F M F M F No accession fee applied M F No accession fee applied University of Nebraska Veterinary Diagnostic Fee Schedule Page 3

7 BACTERIOLOGY Aerobic Culture $12.50/culture 1) swab 2) affected tissue 3) body fluid Anaerobic Culture $12.50/culture 1) swab 2) affected tissue 3) body fluid Salmonella sp. Enrichment $10.00/culture 1) feces 2) affected tissue Brachyspira hyodysenteriae $ Feces 2 Fecal swab 1) culturette 2) sterile 1)anerobic transport media 2) sterile 1) Sterile 1) culturette 2) sterile 1) none 2) frozen gel M F 2 5 days Samples should be kept cool and moist in transit. M F 2 5 days Ship overnight. M F 2 5 days Samples should be kept cool and moist in transit. M F 3 7 days Samples should be kept cool and moist in transit. Antiobiotic Susceptibility Test Acid fast Stain for Mycobacterium sp., without culture Actinobacillus pleuropneumoniae or S. Suis serotyping Campylobacter culture (intestinal and fecal) Campylobacter culture (reproductive) Culture Isolate Slant request** $6.00/organism $5.00/sample 1) feces 2) affected tissue 1) Sterile $12.50/organism Live organism 1)agar plate or slant $12.50/culture $12.50/culture $12.50/slant, plus shipping 1)intestine 2)feces 1)uterine swab 2) preputial wash 1) Sterile 2) CMMA 1) Sterile 2) CMMA 1)frozen gel 2)none 1)frozen gel 2)none M F 1 2 days Samples should be kept cool and moist in transit. M F 1 2 days Client must include a copy of UNL VDC s USDA permit to ship vectors. Call lab for a copy. M F 2 5 days M F 4 6 days Samples should be kept cool and moist in transit. M Th **To assure a viable slant, please request slant within 5 days of final report. Client must provide the VDC with a copy of their USDA permit to ship vectors for the destination location. University of Nebraska Veterinary Diagnostic Fee Schedule Page 4

8 (Bacteriology Cont d.) Fungal Culture $12.50/sample 1) swab 1) culturette 2) affected 2) sterile tissue 3) body fluid Fluorescent Antibody (FA) test for Clostridium novyi, chauvoei, septicum, or sordeli Fluorescent Antibody (FA) test for Leptospira sp. Johne s (M. paratuberculosis) Culture, solid or liquid includes PCR confirmation of positives $12.50/sample 1)muscle 2) sterile $15.00/sample 1)urine 2)kidney 2) sterile $25.00/sample 1)feces 2) sterile Listeria Cold Enrichment $8.00/sample 1)brain stem 1) sterile Milk Culture $10.00/culture Milk 1) sterile Mycoplasma Culture $15.00/culture (Bovine/Ovine/Caprine) 1) swab 2) affected tissue 3) body fluid 1) culturette 2) sterile M F 1 4 weeks Samples should be kept cool and moist in transit. M F 1 2 days Samples should be kept cool and moist in transit. M F 1 2 days Samples should be kept cool and moist in transit. M W Samples should be kept cool in transit. weeks M F 6 weeks Samples should be kept cool and moist in transit. M F 2 3 days Samples should be kept cool in transit. M F 1 4 weeks Samples should be kept cool and moist in transit. University of Nebraska Veterinary Diagnostic Fee Schedule Page 5

9 BACTERIOLOGY MOLECULAR DIAGNOSTICS (PCR TESTING): Bacillus anthracis $25.00/sample Spleen 1) sterile Brachyspira pilosicoli $25.00/sample Feces or 1) sterile colon Chalmydia $25.00/sample 1) swab 2) affected tissue 3) body fluid Clostridium perfringens $25.00/sample Live (toxin typing from culture) organism E. coli typing (pili,toxin) $25.00/sample Live form culture organism Johne s (M. paratuberculosis) Direct Fecal Lawsonia intracellularis (PPE) 1) culturette 2) sterile $22.00/sample feces 1) sterile $25.00/sample Intestinal scrapings; feces Leptospira sp. $25.00/sample 1)Urine 1) sterile 1) sterile 2)Kidney Listeria $25.00/sample Brain stem 1) sterile Mycoplasma PCR General $25.00/sample 1) swab 1) culturette 2) affected 2) sterile tissue 3) body fluid As Needed As Needed W T & Th T & Th M W T & Th W T & Th 48 hours 48 hours Organism must be grown on culture before PCR can be performed 48 hours 48 hours 48 hours 48 hours 48 hours 48 hours 48 hours 48 hours University of Nebraska Veterinary Diagnostic Fee Schedule Page 6

10 BACTERIOLOGY MOLECULAR DIAGNOSTICS (PCR TESTING): (cont d.) Mycoplasma bovis speciation $25.00/sample 1) swab 2) affected tissue 3) body fluid Tritrichomonas foetus 25.00/sample Preputial wash; vaginal wash 1) culturette 2) sterile InPouch Ambient temperature NO ICE PACKS T & Th 48 hours T &F 48 hours InPouches are incubated for a minimum of 24 hours after arrival in the lab before testing. All samples not sent in InPouches will be inoculated to media in the lab at your cost ($4.50/InPouch plus $3.50/inoculation fee) Ureaplasma sp. $25.00/sample 1) swab 2) affected tissue 3) body fluid 1) culturette 2) sterile Tue & Thurs 48 hours University of Nebraska Veterinary Diagnostic Fee Schedule Page 7

11 BACTERIOLOGY PARASITOLOGY: Acid fast stain for Cryptosporidia sp. $7.00/sample Feces 1) sterile 2) 1:1 ratio of feces to formalin Fecal Floatation $7.00/sample Feces 1) sterile Trichomonas foetus in $7.00/culture Preputial In Pouch pouch (incubate & one wash; read) vaginal wash Trichomonas foetus, not in pouch (inoculate, incubate, and one read) Giardia/Cryptosporidium Antigen Parasite gross or microscopic identification $15.00/culture Preputial wash; vaginal wash 1) sterile $22.00/sample Feces 1) sterile 2) 1:1 ratio of feces to formalin $7.00/sample Parasite or organism 1) sterile Ambient temperature NO ICE PACKS Ambient temperature NO ICE PACKS M F M F 24 hours 24 hours M F 4 6 days Samples are incubated for 4 6 days before they are read M F 4 6 days Pouches are inoculated and then samples are incubated for 4 6 days before they are read M F 24 hours Room Temp M F 24 hours University of Nebraska Veterinary Diagnostic Fee Schedule Page 8

12 BACTERIOLOGY SEROLOGY: Bovine: Brucella abortus $1.00/sample Serum Leptospira (6 serovars) $9.00/sample Serum Canine: Brucella canis (Card test) $12.00/sample Serum Brucella canis (IFA test) $15.00/sample Serum Ehrlichia canis (IFA test) $13.00/sample Serum Leptospira (6 serovars) $9.00/sample Serum Lyme Disease (IFA test) $13.00/sample Serum Rocky Mountain Spotted $13.00/sample Serum Fever (RMSF) Tick Screen (E. canis, Lyme, RMSF) $35.00/sample Serum Porcine: Brucella suis $1.00/sample Serum Leptospira (6 serovars) $9.00/sample Serum Daily 24 hrs. $0.50 charge to process blood tubes (spin & remove serum) W 5 days M F 24 hrs. If card test positive, IFA test will be run at no additional charge M F 24 hrs. M F 24 hrs. W 5 days M F 24 hrs. M F 24 hrs. M F 24 hrs. Daily 24 hrs. $0.50 charge to process blood tubes (spin & remove serum) W 5 days University of Nebraska Veterinary Diagnostic Fee Schedule Page 9

13 If your poison of interest is not listed or you need further information about the availability of analytical services, please contact the Toxicology TOXICOLOGY: Laboratory staff at (July 16, 2007) TEST (analytical service) FEE SPECIMEN SHIPPING Bone Ash & Density (gravimetric) $10.00/specimen Bone Chilled or frozen M F 1 3 working days Turn around time may be lengthened if extensive bone clean up is required before anaysis Cholinestease (modified Ellman method) Calcium and magnesium (atomic absorption spectrophotometry) $15.00/specimen 1 st specimen $20.00; additional specimens $10.00 Whole blood, brain, retina Frozen M F 1 3 working days Serum, ocular fluid Frozen M F 1 3 working days Do not freeze whole blood of other analyses are to be performed on that specimen. Identification of organophosphate or carbamate insecticide at additional expense Hemolyzed specimen is unsuitable for analysis. Do not ship specimen on clot. Calcium and phosphorus in bone (ICP) Carbamate insecticides Chlorinated insecticides Copper, Cu (atomic absorption spectrophotometry) See comments Send out Send out 1 st specimen 20.00, additional specimens, $10.00 Rib or long bone from extremity Liver, kidney, rumen/stomach contents, suspected source, urine Brain, body fat, liver, kidney, rumen/stomach contents, suspected source, urine Frozen M F 2 work weeks Remove muscle and send the entire bone. Requires ashing of the bone. Turn around time may be lengthened if extensive bone clean up is required before analysis. Specimen preparation in our laboratory; analysis is a send out Frozen M F Cholinesterase activity often conducted to rule this out. Call Toxicology Laboratory staff for details. Frozen Kidney, liver, serum Frozen M F 1 3 working days Call Toxicology Laboratory staff for details. Not part of a trace mineral panel. Hemolysis does not affect copper results, but it is better not to ship serum on the clot. University of Nebraska Veterinary Diagnostic Fee Schedule Page 10

14 TOXICOLOGY: (cont d.) TEST (analytical service) FEE SPECIMEN Ethylene Glycol/Antifreeze (UV vis spectropotometry) Feed analysis for nutrient content Heavy Metals in tissues (ICP) Herbicides Insecticides Ionophores panel (monensin, lasalocid, salinomycin) Lead,blood (atomic absorption spectroscopy/ MIBK): Unclotted Whole Blood Clotted Blood or tissues Liver Biopsy trace mineral panel 1 st specimen $15.00, additional specimens $5.00 Send out Send out Send out See carbamates, organophosphate s, chlorinated insecticides Send out $25.00 Urine, serum, stomach content, rumen content, source Feed, forages, silages, mixed rations, concentrates Kidney*, liver, stomach content, rumen content, source Consult with toxicologist Stomach content, rumen content, source Heprinized or EDTA whole blood preferred SHIPPING Frozen M F 1 3 working days Frozen or chilled Legal cases may require confirmation at additional cost. Call Toxicology Laboratory staff for details. M F Nutritional panels, proximate analyses, minerals, vitamins, feed additives. Call Toxicology Laboratory staff for details. Frozen *Kidney is the preferred specimen. Call Toxicology Laboratory staff for current information Frozen M F Call VDC toxicologist for details Toxin screen by GC/MS may be the initial analysis performed in cases of acute insecticide toxicoses Call Toxicology Laboratory staff for current information Kidney is the preferred specimen for tissues. $30.00 Send out Call for protocol Call Toxicology Laboratory staff for current information Melamine and related compounds, qualitative only (gas chromatography/mass spectroscopy) $100 per specimen Pet food Chilled M F Depends upon demand Offered in cooperation with the UNL Water Science Laboratory. Call Toxicology Laboratory staff for more information. University of Nebraska Veterinary Diagnostic Fee Schedule Page 11

15 TOXICOLOGY: (cont d.) TEST (analytical service) FEE SPECIMEN Mycotoxin screen 1 st specimen Grains, forages, aflatoxin, ochratoxin A, $30.00, additional silages zearalenone, specimens deoxynivalenol (DON, $20.00 vomitoxin) (Thin layer chromatography) Mycotoxin screen trichothecenes, aflatoxin, fumonisin (gas chromatography/mass spectroscopy, highperformance liquid chromatography) Nitrate & Nitrite in Biofluids (ion chromatography) Nitrate, Forage (specific ion electrode) Organophosphate insecticides Poisonous plant identification (visual, microscopic) Send out 1 st specimen $15.00, additional specimens $9.50 each Grains, forages, silages Ocular fluid, plasma, serum, water SHIPPING Chilled or frozen (wet specimens) Chilled or frezen (wet specimens) $15.00 Forage **See Comment Send out Send out Liver, kidney, urine, stomach/rumen content, suspected source Rumen/stomach content, suspected plant M F 1 3 working days Additional fees for forages or silages may be charged. Confirmation of positive results at additional expense may be required. Results not suitable for interstate commerce. M F Results may not be attainable for some specimens, due to chemical interferences. Call Toxicology Laboratory staff for details. Frozen M F 1 3 working days Ocular fluid is the preferred post mortem specimen. Submit the intact eyeglobe if you desire us to remove the ocular fluid for analysis **Dry specimens may be shipped without further preservation. Green or wet specimens should be shipped frozen. University of Nebraska Veterinary Diagnostic Fee Schedule Page 12 M F Normally 1 3 working days (depends on drying time) Do not send root balls if sending the whole plant. Results reported on a dry weight basis. Very wet or fresh plants may require 2 3 days to dry. Frozen M F Cholinesterase activity often conducted to rule this out. Call Toxicology Laboratory staff for details. Do not freeze M F Call Toxicology Laboratory staff for details.

16 TOXICOLOGY: (cont d.) TEST (analytical service) FEE SPECIMEN Selenium (ICP) Call laboratory for Liver, whole current details blood, serum, feed SHIPPING Frozen M F May be part of trace mineral panel. Call Toxicology Laboratory staff for current information Strychnine (thin layer chromatography) Total dissolved solids (TDS) (gravimetric) Toxin screen by gas chromatography/mass spectroscopy $45.00 Liver, kidney, stomach/rumen content, urine, suspected source 1 st specimen $5.00, additional specimens $2.50 Send out Frozen M F 1 3 working days Water Chilled M F 1 3 working days Liver, kidney, rumen/stomach content,urine, suspected source Call Toxicology Laboratory staff for current information Trace mineral panel (ICP) Call for details, Send out Liver, serum, feed or diet Frozen M F Do not assume selenium is included. Call Toxicology Laboratory staff for details. Vitamins A and E Send out Liver, serum Frozen M F Hemolysis may render the specimen unsuitable. Do not ship serum on the clot. Water quality panel (livestock) (bromide, chloride, nitrate, nitrite, phosphate, sulfate) (ion chromatography) $16.00 Water Fresh M F 5 working days Offered in cooperation with the UNL Water Science Laboratory. (Interpretation is for livestock use, not human use) University of Nebraska Veterinary Diagnostic Fee Schedule Page 13

17 VIROLOGY: LARGE ANIMAL: Antigen Capture ELISA BVDV (serum) $5.00/sample Serum Bovine Rota Virus $15.00/sample Feces Coronavirus (feces) $15.00/sample Feces Electron Microscopy (neg. $22.50/sample Feces stain) Failure of Passive Transfer See Comments (FPT) Rabies Testing [FA] (nonhuman exposure $30.00/sample per head Entire head Wildlife Surveillance Samples $ 5.00/brain Entire brain, fresh Virus Isolation in cell $25.00/single Tissue culture (cell lines to support sample Serum (PRRS) bovine, porcine virus and select small animal and equine viruses) COMPANION ANIMAL: EM negative stain fecal $22.50 Feces Rabies Testing [FA] (nonhuman exposure) Wildlife Surveillance samples Isolation (call for specific availability) $20.00/brain; $30.00/head $ 5.00/brain Entire brain, fresh/entire head Entire brain, fresh $25.00 As needed 48 hrs. As needed 48 hrs. As needed 48 hrs. As needed hrs. As needed 24 hrs. Cattle $5.00; Horses, llamas, alpacas $10.00 As needed 24 hrs. Tuesdays 2 wks. Samples must arrive at lab 24 hrs. prior to As needed hrs. As needed As needed 24 hrs. 2 wks. University of Nebraska Veterinary Diagnostic Fee Schedule Page 14

18 VIROLOGY MOLECULAR DIAGNOSTICS (PCR): Avian Influenza Real time PCR Bovine Viral Diarrhea (BVD) Realtime (Screen) $25.00/sample $25.00/sample Cloacal & tracheal swabs (1 each) Serum, ear notch, lymphoid tissue BVD Typing $45.00/sample Bovine Respiratory Syncytial $25.00/sample Nasal swab or Virus (BRSV) PCR lung tissue Canine Distemper Virus $25.00/sample Conjunctival (CDV) scrapings, urine, whole blood, CSF Canine Influenza $25.00/sample (Screening) Classical Swine Fever (CSF) Realtime $25.00/sample Tonsil, nasal swabs Circovirus PCR $35.00/sample Purple top tube Equine Viral Arteritis (EVA) $25.00/sample Nasopharyngeal Realtime & conjunctival swabs & semen Equine Herpes Virus 1 (EHV 1) Realtime Feline Infectious Peritonitis (FIP) PCR $25.00/sample Nasal swabs $25.00/sample Abdominal fluid/tonsil lymphoid tissue; liver, kidney from dead animal ; Call lab for viral transport media University of Nebraska Veterinary Diagnostic Fee Schedule Page 15

19 VIROLOGY MOLECULAR DIAGNOSTICS (PCR): (cont d.) Foot and Mouth (FMD) Realtime Malignant Catarrhal Fever (MCF) PCR $25.00/sample Call lab $25.00/sample Lymphoid tissue or purple top tube IBR Realtime $25.00/sample Nasal swabs, lung Neospora PCR $25.00/sample Brain, skeletal muscle, heart Newcastle Disease Virus $25.00/sample Cloacal swabs (NDV) Realtime Parvo PCR $25.00/sample Porcine Reproductive and Respiratory Syndrome (PRRS) PCR PRRS RFLP/Sequencing (on viral isolate) Pseudorabies Virus (PRV) Single Reactor PCR $25.00/sample Serum, lymphoid tissue $150.00/sample Viral isolate $50.00/sample Call lab Q Fever $25.00/sample Placenta, aborted fetus Swine Influenza Virus (SIV) $25.00 for Lung screening West Nile Virus (WNV) Realtime $25.00 Call VDC PCR As needed 2 wks. Samples must be in lab 24 hrs. prior to University of Nebraska Veterinary Diagnostic Fee Schedule Page 16

20 VIROLOGY SEROLOGY: (Volume discount may be applied to some serology tests please inquire) AVIAN: Avian Influenza Virus [ELISA] Serum $2.00/sample (no accession fee) Hemorrhagic Enteritis Virus [ELISA] $2.00/sample Serum Infectious Bronchitis Virus [ELISA] $2.00/sample Serum Newcastle Disease Virus (NDV T & $2.00/sample Serum NDV C) [ELISA] Mycoplasma MG, MS [ELISA] $2.00/antigen/ Serum sample Salmonella pullorum Microtiter $4.00/test Serum West Nile Virus [ELISA IgM] $10.00/sample Serum West Nile Virus [ELISA, IgG] $10.00/sample Serum As needed 24 hrs. Samples must arrive at lab 24 hrs. prior to As needed hrs. Samples must arrive at lab 24 hrs. prior to As needed hrs. Samples must arrive at lab 24 hrs. prior to As needed hrs. Samples must arrive at lab 24 hrs. prior to As needed hrs. Samples must arrive at lab 24 hrs. prior to As needed hrs. Samples must arrive at lab 24 hrs. prior to As needed hrs. Samples must arrive at lab 24 hrs. prior to As needed hrs. Samples must arrive at lab 24 hrs. prior to BOVINE: (0.5 ml/virus) FEE SPECIMEN SHIPPPING Anaplasma marginale [ELISA] See Comments Serum Bluetongue Virus [AGID or ELISA] $5.00/sample Serum Bovine Leukemia Virus (BLV) [AGID or ELISA] See Comments Serum Bovine Parainfluenza 3 Virus [SN] $6.00/sample Serum Bovine Respiratory Syncytial Virus $6.00/sample Serum (BRSV) [SN] As hrs. $10.00/sample for 1 3 samples; needed $5.00/sample for 4 or more samples. Samples must be at lab by 10:30 a.m. As 3 days for Samples must be at lab by 10:30 a.m. needed AGID; hrs. for ELISA As 3 days for 1 44 samples: $5.00/sample needed AGID; samples: $4.00/sample 48 hrs. for 94+ samples: $3.00/sample. ELISA M & F 5 days Samples must arrive at lab 24 hrs. prior to M & F 5 days Samples must arrive at lab 24 hrs. prior to University of Nebraska Veterinary Diagnostic Fee Schedule Page 17

21 VIROLOGY SEROLOGY: (cont d.) BVD, Type I [SN] (Antibody detection BVD Type II [SN] (Antibody detection) IBR (Infectious bovine rhinotracheitis) Virus [SN] Mycobacterium Paratuberculosis (Johne s Disease) [ELISA] $6.00/sample Serum $6.00/sample Serum $6.00/sample Serum See Comments Serum M & F 5 days Samples must arrive at lab 24 hrs. prior to M & F 4 days Samples must arrive at lab 24 hrs. prior to W & F 24 hrs samples: $5.00/sample samples: $4.00/sample 93+ samples: $3.50/sample Neospora caninum [ELISA] See Comments Serum As needed Samples must arrive at lab 24 hrs. prior to 24 hrs samples: $5.00/sample samples: $4.00/sample 93+ samples: $3.00/sample. CANINE: FEE SPECIMEN SHIPPPING West Nile Virus {ELISA IgM] $10.00/sample Serum West Nile Virus [ELISA, IgG] $10.00/sample Serum M & F 5 days Samples must be at lab 24 hrs. prior to testing to As needed hrs. Samples must arrive at lab 24 hrs. prior to As needed hrs. Samples must arrive at lab 24 hrs. prior to CAPRINE: Caprine Arthritis Encephalitis (CAE)/Ovine Progressive Pneumonia (OPP) [AGID] $5.00/sample Serum As needed 3 days Samples must arrive at lab 24 hrs. prior to EQUINE: Coggins Testing ELISA [AGID] $5.00/sample (no accession fee) Serum West Nile Virus [ELISA IgM] $10.00/sample Serum West Nile Virus [ELISA IgG] $10.00/sample Serum Daily 24 hrs. Samples must arrive at lab by 1 p.m. for same day testing. As needed hrs. Samples must arrive at lab 24 hrs. prior to As needed hrs. Samples must arrive at lab 24 hrs. prior to University of Nebraska Veterinary Diagnostic Fee Schedule Page 18

22 VIROLOGY SEROLOGY: (cont d.) PORCINE: APP $4.00/sample Serum M. hyopneumoniae $3.00/sample Serum Porcine Parvovirus [HAI] $6.00/sample Serum Porcine Reproductive and Respiratory Syndrome (PRRS) Virus [ELISA] $4.00/sample Serum PRV Screening (gb) $2.00/sample Serum PRV gi $2.00/sample Serum PRV SN $6.00/sample Serum Swine Influenza Virus (SIV) HAI H 1 $6.00/sample Serum N 1 Swine Influenza Virus (SIV) HAI $6.00/sample Serum H 3 N 2 Transmissible Gastroenteritis Virus $6.00/sample Serum (TGE) SN As needed hrs. T & Th 24 hrs. Samples must arrive at lab by 10:30 a.m. W 48 hrs. Samples must arrive at lab 24 hrs. prior to testing T & Th 24 hrs. Samples must arrive at lab by 10:30 a.m. As needed Samples must arrive at lab by 10:30 a.m. As needed Samples must arrive at lab by 10:30 a.m. As needed Samples must arrive at lab 24 hrs. prior to Wed. Samples must arrive at lab 24 hrs. prior to Wed. Samples must arrive at lab 24 hrs. prior to M or F 5 days Call VDC before submitting samples University of Nebraska Veterinary Diagnostic Fee Schedule Page 19

23 MEDIA AND SUPPLIES: Amies swabs... $1.00 each Anaerobic Transport (Port a Cult) tubes... $4.75/tube Campylobacter transport media... $2.25/tube Trichomonas transport media... $4.50/pouch Viral transport media... Provided at no charge Biopsy mailers... Provided to clients upon request (no charge) FIELD INVESTIGATION (Available through consultation with local veterinarian only) Site visit for pathologist and/or extension investigations. Call for information: LEGAL WORK Legal work (includes paperwork, depositions)... $100.00/hr. Photo documentation... $2.00 per image; $5.00 per printed page MISCELLANEOUS INFORMATION For regular shipping to an outside laboratory, a $12.50 shipping and handling fee will be charged on all specimens sent for testing. If a specimen is shipped overnight, the applicable charges will be applied. Related assays can be developed for research or service needs. Contact the Diagnostic Center for further information. LABORATORY RESULTS Case results are accessible on the Internet. To view case results, please go to: and request a password. University of Nebraska Veterinary Diagnostic Fee Schedule Page 20

24 Results are also FAXed as they become available. Please keep up informed of any new FAX numbers or of any changes to your FAX number. Thank you. Animal Disease Phone/Fax Numbers: Nebraska Veterinary Diagnostic Center: Dairy/Beef Extension: Phone: (402) (402) Fax: (402) E mail: vdc2@unl.edu Federal Veterinarian: Phone: (402) Nebraska State Veterinarian: Fax: (402) Phone: (402) / Fax: (402) Kansas State Univ. Rabies Lab: (785) Nebraska State Veterinarian (Rabies RA#): (402) PLEASE NOTE: When sending specimens via UPS, FedEx, DHL, etc. and printing an address label from a Website, please use the following address: University of Nebraska Veterinary Diagnostic Center 1900 N. 142 nd St. Lincoln, NE When sending specimens via regular mail, to expedite arrival of specimens, please use the following address: University of Nebraska Veterinary Diagnostic Center P. O. Box Lincoln, NE University of Nebraska Veterinary Diagnostic Fee Schedule Page 21