DISEASE SAMPLING. Readings. What to wear, what to wear 3/9/2009. Required. Supplemental. Rubber boots or waders Disposable gloves
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1 DISEASE SAMPLING Readings Required Standard operating procedures SEPARC collecting and shipping specimens for diagnostic testing Green et al. Disease Monitoring and Biosafety Section 26.3 and 26.4 Supplemental Green et al. Disease Monitoring and Biosafety What to wear, what to wear Rubber boots or waders Disposable gloves Latex Nitrile Vinyl 1
2 What to look for Corpses Lethargic or odd behaviors External signs Lesions Edema Erythema Malformations Internal gross signs Discolored or abnormal looking organs Other common tissues Kidneys, liver, lungs, gills, spleen, thymus, skeletal muscle Oddities Collection Collect any corpses Collect specimens that show gross external signs Or specimens that exhibit odd behavior Individuals from various age groups Place individuals into separate bags or containers 2
3 Equipment Disinfection Remove mud Spray w/ disinfectant Dispose of gloves >.75% 1 min. >3% 1 min Next Step? Euthanize any live specimens Using Benzocaine hydrochloride Must be authorized by veterinarian mg/l Mix with 90% ethanol Other approved procedures Tricaine Methanesulfonate (MS222) >250 mg/l Slow cooling Physical methods (only recommended for unconscious animals) Sharp blow to the head Electrocution Decapitation 3
4 For reptiles Consult AAZV Euthanasia of Nondomestic Animals 1) Ketamine HCl or Telazol (DEA) Veterinarian must purchase 2) Sodium pentobarbital (DEA) Veterinarian must purchase 3) Decapitation/gunshot Oh. Crap! Preservation of tissues Fresh tissues are always preferred For diagnostic tests Freeze or preserve in ethanol 90% or greater For histology Formalin fixed tissues 10% buffered formalin Shipping animals For live animals Call diagnostic lab for specific instructions Follow courier s guidelines For specimens Triple package system 4
5 Guidelines for triple packaging Label each layer Do not use biohazard bags unless known infection with Category A substance (human health hazard) Use dry ice only when shipping frozen samples Ethanol and formalin samples don t need dry ice or ice packs Outermost layer Include the label Exempt animal specimen Include shipper and receiver addresses Include other appropriate chemical labels (Dry ice, dangerous goods, etc.) Other materials sent inside package Detailed list of contents Description of where samples originated Contact information of the shipper Requested services Diagnostic testing Histology Serology MSDS Only if BSL-2 materials or regulated substances (formalin or EtOH Shipping fresh/frozen tissues Ship fresh tissues with ice packs ThermoSafe* Polar Pack Dry Ice: Only use dry ice with frozen samples Shipping FedEx: Class 9 dangerous goods label If not included on label mark DRY ICE UN1845 Must indicate weight of dry ice Do not exceed 5 lbs of dry ice 5
6 Shipping dry ice with UPS Only except packages with small amounts of dry ice (<5lbs) DO NOT put class 9 label on outer box Will not accept Only need to state Dry Ice used as refrigerant for diagnostic materials Shipping with ethanol and formalin Both FedEx and UPS require either Dangerous Goods In Excepted Quantity label < 500mL, <30ml/Container Or Dangerous Goods in Hazardous Quantity label >500mL Shipping Label University of Tennessee (Matthew J. Gray, Ph.D.) Department of Forestry, Wildlife and Fisheries Institute of Agriculture 274 Ellington Plant Sciences Building Knoxville, TN USA Debra L. Miller, D.V.M., Ph.D. Veterinary Diagnostic Laboratory The University of Georgia 43 Brighton Road Tifton, GA Contents: Exempt Animal Specimen (Refrigerate upon Arrival) Phone: No statement required for environmental samples Unless shipping with Dry Ice 6
7 Tests Histology Virus isolation Electron microscopy PCR Radiology Clearing-and-staining 7
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1. IDENTIFICATION OF THE SUBSTANCE / MIXTURE AND SUPPLIER Product name: Other names: None Product code: AE F124964 00 1P01 A1 Recommended use: For use in the control of cockroaches in domestic and commercial
Summary of the Death Investigation of Bethany Lynn Stephens Goochland Sheriff s Office February 20, 2018
Summary of the Death Investigation of Bethany Lynn Stephens Goochland Sheriff s Office February 20, 2018 The last time Bethany Stephens was seen alive was at her father s residence at 15:48 on Wednesday,
SUMMARY OF PRODUCT CHARACTERISTICS. Euthasol vet. 400 mg/ml, solution for injection (AT, BE, DK, EE, EL, FI, IE, IS, LT, LU, LV, NO, PL, RO SE, UK)
SUMMARY OF PRODUCT CHARACTERISTICS 1. NAME OF THE VETERINARY MEDICINAL PRODUCT Euthasol vet. 400 mg/ml, solution for injection (AT, BE, DK, EE, EL, FI, IE, IS, LT, LU, LV, NO, PL, RO SE, UK) Euthasol 400