INTERNATIONAL WORKSHOP ON GENETIC RESISTANCE TO PARASITES IN SMALL RUMINANTS

Transcription

1 Program INTERNATIONAL WORKSHOP ON GENETIC RESISTANCE TO PARASITES IN SMALL RUMINANTS September, 22-23, 2012 Gran Canaria SPAIN

2 CHAIRPERSONS COMMITTEES: Jorge F. González (Chairperson, University of Las Palmas de Gran Canaria, Spain) David Piedrafita (Deputy Chairperson, Monash University, Australia) LOCAL ORGANIZING COMMITTEE SECRETARY Rodríguez Eligia (University of Las Palmas de Gran Canaria, Spain) LOCAL ORGANIZING COMMITEE Argüello Anastasio (University of Las Palmas de Gran Canaria, Spain) Andrada Marisa (University of Las Palmas de Gran Canaria, Spain) Batista Miguel (University of Las Palmas de Gran Canaria, Spain) Castro Noemí (University of Las Palmas de Gran Canaria, Spain) Capote Juan (University of La Laguna, Spain) Conde Magnolia (University of Las Palmas de Gran Canaria, Spain) Fernández Antonio J. (University of Las Palmas de Gran Canaria, Spain) Jaber, José R. (University of Las Palmas de Gran Canaria, Spain) Hernández Julia (University of Las Palmas de Gran Canaria, Spain) Hernández Lorenzo E. (University of Las Palmas de Gran Canaria, Spain) López Adassa (University of Las Palmas de Gran Canaria, Spain) Martel Diego (University of Las Palmas de Gran Canaria, Spain) Millán Sara C. (University of Las Palmas de Gran Canaria, Spain) Molina José M. (University of Las Palmas de Gran Canaria, Spain) Morales Antonio J. (University of Las Palmas de Gran Canaria, Spain) Muñoz Mª Carmen (University of Las Palmas de Gran Canaria, Spain) Ortega, Leire (University of Las Palmas de Gran Canaria, Spain) Pérez Davinia (University of Las Palmas de Gran Canaria, Spain) Quesada, Yéssica (University of Las Palmas de Gran Canaria, Spain) Rivero, Fernando (University of Las Palmas de Gran Canaria, Spain) Rodríguez Alejandro B. (Small Ruminant Clinician, Spain) Ramírez Ana S. (University of Las Palmas de Gran Canaria, Spain) Rivero Miguel (University of Las Palmas de Gran Canaria, Spain) Rodríguez Francisco (University of Las Palmas de Gran Canaria, Spain) Ruiz Antonio (University of Las Palmas de Gran Canaria, Spain) INTERNATIONAL ADVISORY COMMITTEE Díaz-Baños, Pablo (University of Santiago de Compostela, Spain) González Jorge F. (University of Las Palmas de Gran Canaria, Spain) Hernández Santiago (University of Córdoba, Spain) Hoste Hervé (INRA, France) Mandonnet Nathalie (INRA, French West Indian) Meeusen Els (Monash University, Australia) Miller James (Louisiana State University, US) Molina José M. (University of Las Palmas de Gran Canaria, Spain) Piedrafita David (Monash University, Australia) Raadsma Herman (Sydney University, Australia) Reina David (University of Extremadura, Spain) Rojo-Vázquez, F. (University of Leon, Spain) Stear Michael (University of Glasgow, Scotland) Torres-Acosta Felipe (University Autonomous of Yucatán, México) Uriarte Joaquín (Agrifood Research and Technology Centre of Aragon, Spain) 2

3 INVITATION TO DELEGATES Dear Colleagues, On behalf of the Organizing Committees, it is a great pleasure to invite you all to attend the International Workshop on Genetic Resistance to Parasites in Small Ruminants ( in Gran Canaria (The Canary Islands, Spain), from 22 nd to 23 rd of September Many collaborators and researchers who attended the 23 rd Conference of the World Association in Advances in Veterinary Parasitology (WAAVP) in Buenos Aires (Argentina) discussed with great interest, the recent progress being made towards animal health and genetics for parasite disease resistance. This has involved the recognition that local indigenous production breeds are an invaluable, unique and underutilised resource for sustainable livestock production. These animals have the potential to lead the discovery of genes and mechanisms of resistance to parasite infections, and to uncover new pharmacological targets for novel chemical prophylaxis and vaccines. Such indigenous breeds will contribute to reduced hunger and poverty and to ensure environmental sustainability (Millennium Development Goal 1 and 7 respectively). In September 2012, Las Palmas de Gran Canaria University has been selected to hold the Conference of the International Goat Association ( We considered this is a timely meeting to also develop this two-day workshop concurrent with the IGA congress. We have confirmed multiple international researchers (including from Australia, the UK, United States, French West Indies and Spain) who are leaders in this field of research as speakers for this workshop. We look forward to welcoming you to Gran Canaria (Spain) and invite you to enjoy an open and stimulating discussion about science in a relaxed picturesque environment. Dr. Jorge F. González Senior Research Fellow University of Las Palmas de Gran Canaria Dr. David Piedrafita Associate Professor Monash University 3

4 SPECIFIC AIMS OF THE WORKSHOP The main aims of this workshop will be: 1. Create an international advisory and research group with interest in genetic resistance to parasites in small ruminants 2. An up-date in phenotypic and genetic breed resistance progress. This information should be incorporated into DAD-IS systems 3. Open discussions with researchers, extension agents, local professional from both developed an developing countries about the use of these breeds and recommendations about drug applications in the field 4. A discussion around the biotechnology industry. What the pharmacological industry could learn from these breeds and how these data could help to solve some parasitological problems in the World. LOCATION AND DATES The International Workshop on Genetics Resistance to Parasite Infections in Small Ruminants will take places in Arucas, Gran Canaria (The Canary Islands, Spain) ( Gran Canaria is a lovely volcanic island located in the Atlantic Ocean about 150 km from northwest cost of Africa; geographically, in the centre of the Canary Islands Archipelago. It is a very popular tourist destination in Europe, with more than 2 million tourists per year. Although it is a tiny island, with only 1,560 km 2, the island is called, miniature continent due to the different climates and varieties of landscapes found, with long beaches and dunes of white sand, contrasting with green sceneries, pine forest and beautiful villages. The Workshop will take places at the School of Veterinary Sciences of the University of Las Palmas de Gran Canaria. Delegates will have free access to internet (wifi) in this facility. Dates will be 22 nd 23 rd of September,

5 LANGUAGE The official language of the Workshop will be English CLIMATE The weather in Las Palmas de Gran Canaria in September is usually sunny with historical temperatures ranging from 21.2 to 27.1 ºC. TIME ZONE The time zone in Las Palmas de Gran Canaria is GMT. 5

6 9:00-9:30 Registration WORKSHOP PROGRAM 9:30-10:30 Opening ceremony Welcome speeches: Jorge Orós (Dean of the Veterinary Faculty, University of Las Palmas de Gran Canaria) Jorge F. González or David Piedrafita (Chairpersons) Antonio Osuna (President of the Spanish Society of Parasitology) Juan Pedro Dávila de León (Director General de Ganadería, Canary Goverment) Marina Villegas Gracia (Subdirectora General de Proyectos de Investigación, Department of Economy and Competitive, Spanish National Governement) Irene Hoffman (Chief, Animal Genetic Resources Branch, FAO) Antonio J. Fernández (Head of the Institute of Animal Health and Food Security of the University of Las Palmas de Gran Canaria) 10:30-11:00 Coffee & Tea break 11:00-13:00 Session 1: Breeding for resistance against parasites in small ruminants I Speakers 11:00-11:30 Gulf Coast Native, a local breed of sheep with enhanced resistance against gastrointestinal nematodes. An update James Miller 11:30-12:00 Canaria Hair Breed Sheep: Identification of a new mechanism of resistance against gastrointestinal nematodes. An update Jorge F. González 12:00-12:30 Increased production through parasite control: can ancient breed of sheep teach us new lessons? David Piedrafita 12:30-13:00 The resistance of the Spanish Churra sheep breed to the infection by gastrointestinal nematodes Francisco A. Rojo 13:00-14:00 Lunch 14:00-15:30 Session 2: Genetics resistance against parasites in small ruminants. Biotechnology 14:00-14:30 Creole goats a local breed of goat with enhanced resistance against gastrointestinal nematodes Nathalie Mandonnet 14:30-15:00 Genomics approach for characterizing parasite resistance Herman W. Raadsma 15:00-15:30 Immunogenetic analysis of resistance of nematode infections Michael Stear 6

7 15:30-16:00 Immunological mechanisms of resistance against gastrointestinal nematode parasites in sheep. Els Meeusen 16:00-16:30 Coffee break 16:30-17:00 Session 3. General poster sessions: alternative methods for the control of parasite infections in small ruminants 17:00-17:30 Round table: Parasite resistance in sheep and goats. Local and biotechnology approach. (Open discussions with all delegates) Chairman: Rojo and González Speakers: Miller, Piedrafita, Mandonnet, Raadsma, Stear and Meeusen 17:30-18:00 Conclusions of the Workshop 23 rd of September 9:00-11:00 Cultural visit to Arucas. Local authorities reception. 10:30-11:00 Coffee & Tea break 11:00-13:30 Technical visit. Herds of Canaria Hair Breed sheep and Canaria sheep 13:30-14:45 Lunch 14:45-15:00 Closing ceremony 7

8 KEY SPEAKER PROFILES Dr Jorge Francisco González Senior Research Fellow Project Leader: Genetic resistance to nematodes in small ruminants Parasitology Unit. Department of Animal Pathology Veterinary Faculty, University of Las Palmas de Gran Canaria, Arucas Canary Island, Las Palmas (Spain) Phone: BRIEF BIOGRAPHY Dr. Jorge F. González obtained his first degree in Veterinary Sciences in 1996 at the University of Las Palmas de Gran Canaria (ULPGC). He was awarded a competitive National Fellowship at the Department of Animal Pathology at the ULPGC in However, by September, 1997, he was appointment Associate Professor at the Parasitology Unit of the Veterinary Faculty of ULPGC and embarked on a PhD about immune response against gastrointestinal nematodes in goats within this research group. He was appointment Senior Research and Lecturer Fellow in He has visited several national and international research laboratories and he has established collaborative work with many of them. He is the principal investigator for several competitive and industry supported grants. His current main research aim is identified why the Canaria Hair Breed sheep is more resistant to gastrointestinal nematode infections than local Canaria sheep. SELECTED PUBLICATIONS - Jorge F. González, Álvaro Hernández, Els N.T. Meeusen, Francisco Rodríguez, José M. Molina, José R. Jaber, Herman Raadsma and Piedrafita, D. (2011). Fecundity in adult Haemonchus contortus parasites is correlated with abomasal tissue eosinophils and gamma-delta T cells in resistant Canaria Hair Breed sheep. Veterinary Parasitology 178: Piedrafita D, Gonzalez J, Raadsma HW & Meeusen ENT (2010). Increased production through parasite control: can ancient breeds of sheep teach us new lessons? Trends in Parasitology 26(12): Molina, J.M., Hernádez, Y., Ruiz, A., González, J.F., Argüellos, A., Ferrer, O., Forbes, A.B., (2009). Preliminary study on the use of a Teladorsagia circumcincta bulk milk ELISA test in dairy goats under experimental conditions. Veterinary Parasitology, 166: Molina, J.M., Ruiz, A., Hernández, B., González, J.F., Martín, S., Hernández, Y.I. (2008). Eprinomectin pour-on and the shedding of Haemonchus contortus eggs in experimentally infected goats. Australian Vet. J., 86: González, J.F., Hernández, A., Molina, J.M., Fernández, A.J., Raadsma, H.W., Meeusen, E.N.T., Piedrafita, D. (2008). Comparative experimental Haemonchus contortus infection of two sheep breeds native to the Canary Islands. Veterinary Parasitology, 153: Molina, J.M., Ruiz, A., Fuentes, P., González, J.F., Martín, S., Hernández, Y.I., Persistent efficacy of doramectin against Haemonchus contortus in goats. The Veterinary Record, 136: Ruiz, A., Molina, J.M., González, J.F., Conde de Felipe, M., Martín, S., Hernández, Y.I Immunoprotection in goats against Haemonchus contortus alter immunization with cysteine protease enriched fractions. Veterinary Research, 35: González, J.F., Molina, J.M., Ruiz, A., Conde de Felipe, M., Rodríguez, E. (2003). The immune response against gastrointestinal nematodos in ruminants: A review. Research and Review in Parasitology, 63: Ruiz, A., Molina, J.M., González, J.F., Martínez-Moreno, F.J., Gutiérrez, P.N., Martínez-Moreno, A. (2003). Humoral response (IgG) of goats experimentally infected with Fasciola hepatica against cysteine protinases of adult fluke. Veterinary Research, 34: Molina, J.M., Ruiz, A., Rodríguez, E., Gutiérrez, A.C., González, J.F., Hernández, S. (1999). Crossreactive antigens of Haemonchus contortus adult worms in Teladorsagia circumcincta infected goats. Veterinary Research, 30:

9 Dr. Nathalie Mandonnet Deputy Director: Animal Research Unit Institut National de la Recherche Agronomique Unité de Recherches Zootechniques Domaine Duclos, Petit-Bourg French West Indies (France) Phone: BRIEF BIOGRAPHY In 1991, Dr. Nathalie Mandonnet was appointment Research Assistant at INRA in Tours where she developed her PhD studies. She returned to French West Indies in 1995 to work in the selection of goat adapted to tropical humid ecosystem within the research group of Physiology and Genetics adaptation to Stress belonging to Zoo technical Research Unit. Her first studies showed genetic variability of productive trait and the adaptability of local animals, in particular its resistance or susceptibility to gastrointestinal nematodes. Since 2000, she has focused her research on identify mechanisms and genes implicated on this greater breed resistance. SELECTED PUBLICATIONS - Gunia, M, Phocas, F, Arquet, R, Alexandre, G, Mandonnet, N. (2011). Genetic parameters for body weight, reproduction and parasite resistance traits in Creole goats. J. Anim. Sci., 89: Bambou, J.C., Archimède, H., Arquet, R., Mahieu, M., Alexandre, G., González-García, E., Mandonnet, N. (2011). Effect of dietary supplementation on resistance to experimental infection with Haemonchus contortus in Creole kids. Vet. Parasitol., 178: Bambou, J.C., Arquet, R., Archimède, H., Alexandre, G., Mandonnet, N., González-García, E. (2009). Intake and digestibility of naïve kids differing in genetic resistance and experimentally parasitized (indoors) with Haemonchus contortus in two successive challenges. J. Anim. Sci. 87: Bambou, J.C., de la Chevrotière, C., Varo, H., Arquet, R., Kooyman, F.N., Mandonnet, N. (2008). Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus. Vet. Parasitol., 158: Alexandre, G., Leimbacher, F., Maurice, O., Domarin, D., Naves, M., Mandonnet, N. (2008). Goat farming systems in Martinique: Management and breeding strategies. Trop. Anim. Health Prod. 41: Mahieu, M., Arquet, R., Kandassamy, T., Mandonnet, N., Hoste, H. (2007). Evaluation of targeter drenching using Famacha method in Creole goat: reduction of anthelmintic use, and effects on kid production and pasture contamination. Vet. Parasitol., 146: Mandonnet, N., Bachand, M., Mahieu, M., Arquet, R., Baudron, F., Abinne-Molza, L., Varo, H., Aumont, G. (2005). Impact on productivity of peri-parturient rise in fecal egg counts in Creole goats in the humid tropics. Vet. Parasitol., 134: Mandonnet, N., Ducroq, V., Arquet, R., Aumont, G. (2003). Mortality of Creole kids during infection with gastrointestinal strongyles: a survival analysis. J. Anim. Sci., 81: Mandonnet, N., Aumont, G., Fleury, J., Arquet, R., Varo, H., Gruner, L., Bouix, J., Khang, J.V. (2001). Assessment of genetic variability of resistance to gastrointestinal nematode parasites in Creole goats in the humid tropics. J. Anim. Sci., 79: Mandonnet, N., Aumont, G., Fleury, J., Gruner, L., Bouix, J., Khang, J.V. (1996). Genetic variability in resistance of Creole goats to natural infection with trichostronylids in Guadeolupe. Ann. Y. Acad. Sci., 23:

10 Professor Els Meeusen Director: Biotechnology Research Laboratories School of Biomedical Sciences, Monash University,Clayton, 3800, Australia Phone: BRIEF BIOGRAPHY Professor Els Meeusen obtained her first degree in Biology at the University of Ghent, Belgium. She worked for two years as an Associate Expert for the UN's Food and Agriculture Organisation in Uruguay, before embarking on a PhD at the University of Cambridge, UK. In 1983, she emigrated to Australia and was an active researcher at the University of Melbourne for the next 22 years, the last 5 years as Director of the Centre for Animal Biotechnology. In January 2006, she was appointed Professor in the Department of Physiology at Monash University, where she continues her efforts to integrate a whole body approach to science into practical applications for the biotechnology and animal and human health industries. Her main areas of interest include: the immunobiology of parasite infections, vaccine development, allergies, and translation of research findings.. SELECTED PUBLICATIONS - Sou T, Meeusen E.N., de Veer M, Morton D.A.V., Kaminskas L.M. and McIntosh M.P. (2011). New developments in dry powder pulmonary vaccine delivery. Trends in Biotechnology 29: Piedrafita D, Raadsma HW, Gonzalez J and EN Meeusen. Increased production through parasite control: can ancient sheep breeds teach us new lessons? Trends in Parasitology (12): de Veer M, Kemp J, Chatelier J, Elhay MJ, Meeusen EN The kinetics of soluble and particulate antigen trafficking in the afferent lymph, and its modulation by aluminum-based adjuvant. Vaccine (40): Fox A, Maddox JF, de Veer MJ, Meeusen EN. (2010). GammadeltaTCR+ cells of the pregnant ovine uterus express variable T cell receptors and contain granulysin. Journal of Reproductive Immunology 84: Young AR, Barcham GJ, Kemp JM, Dunphy JL, Nash A and ENT Meeusen. Functional characterization of an eosinophil-specific galectin, ovine galectin-14 (2009). Glycoconjugate Journal 26: Meeusen E. N. T., Jungersen G., Walker J. and P.P. Pastoret. Current Status of Veterinary Vaccines. Clinical Microbiology Reviews.Clinical Microbiology Reviews. 20(3): Dunphy, J.L., Barcham, G.J., Bischof, R.J., Nash, A, Young A.R. and Meeusen E.N.T.(2002) Isolation and characterization of a novel eosinophil-specific galectin released into lung fluid in response to allergen-challenge. Journal of Biological Chemistry 277: Dunphy, J.L., Balic, A., Barcham, G.J., Horvath, A.J., Nash, A.D., and Meeusen, E.N.T. (2000). Isolation and Characterization of a Novel Inducible Mammalian Galectin. Journal of Biological Chemistry 275(41): Meeusen, E.N.T. and Balic, A. (2000) Do eosinophils have a role in the killing of helminth parasites? Parasitology Today, 16: Meeusen, E.N.T., Premier, R.R. and Brandon, M.R. (1996). Tissue-specific migration of lymphocytes: a key role for Th1 and Th2 cells? Immunology Today 17:

11 Professor James Miller Everett D. Besch Professor of Veterinary Medicine Interim Associate Dean for Research and Advances Studies Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Baton Rouge, LA 72927, USA Phone: BRIEF BIOGRAPHY Dr. James E. Miller has been a member of the faculty since 1984; he was appointed full Professor in 1995 and was Acting Head of the former Department of Epidemiology and Community Health ( ). He is author and/or coauthor of over 90 refereed journal articles, numerous technical/report papers, proceedings papers, and abstracts, and 7 book chapters. He is a research collaborator with numerous national and international organizations. He is a co founder of the Southern Consortium for Small Ruminant Parasite Control. The Consortium s primary purpose is to evaluate and promote alternative non chemical methods for controlling nematode parasites. Dr. Miller is the principal investigator for several competitive and industry supported grants dealing with the epidemiology, control, and genetics of ruminant nematode parasitism. His research program focuses on improving ruminant production using an integrated approach to controlling parasites. His area of special expertise is small ruminant gastrointestinal nematode parasitism, which is the most serious constraint affecting production world wide. Economic losses are caused by decreased production, costs of prophylaxis, costs of treatment, and the death of infected animals. Anthelmintic resistance in nematode populations (specifically affecting small ruminants) threatens the success of treatment programs. Dr. Miller's research is directed at developing alternative strategies for control. His current research program is two-fold: 1) To determine why, and/or how, Gulf Coast Native sheep are relatively more resistant to gastrointestinal nematode infection than Suffolk sheep, and 2) To evaluate alternative (non chemical) approaches for protection against nematode infection. SELECTED PUBLICATIONS - Shakya, K.P., Miller, J.E., Lomax, L.G., Burnett, D.D., Evaluation of immune response to artifical infections of Haemonchus contortus in Gulf Coast Native compared with Suffolk lambs. Vet. Parasitol., 181: Shakya, K.P., Miller, J.E., Horohov, D.W., A Th2 type of immune response is associated with increased resistance to Haemonchus contortus in naturally infected Gulf Coast Native lambs. Vet Parasitol 163, Hunt, P.W., McEwan, J.C., Miller, J.E., Future perspectives for the implementation of genetic markers for parasite resistance in sheep. Trop Biomed 25, Miller, J.E., Bishop, S.C., Cockett, N.E., McGraw, R.A., Segregation of natural and experimental gastrointestinal nematode infection in F2 progeny from susceptible Suffolk and resistant Gulf Coast Native sheep and its usefulness in assessing genetic variation. Vet Parasitol 140, Jackson, F., Miller, J.E., Alternative approaches to control Quo vadis. Vet Parasitol 139, Peña, M.T., Miller, J.E., Horohov, D.W., Effect of CD4+ T lymphocytes depletion on resistance of Gulf Coast Native lambs to Haemonchus contortus infection. Vet Parasitol 138, Miller, J.E., Horohov, D.W., Immunological aspects of nematode parasite control. J Anim Sci 84 Suppl E Peña, M.T., Miller, J.E., Horohov, D.W., Effect of dexamethasone treatment on the immune response of Gulf Coast Native lambs to Haemonchus contortus infection. Vet Parasitol 119: Burke J.M., Miller, J.E., Relative resistance of Dorper crossbred ewes to gastrointestinal nematode infection compared with St. Croix and Katahdin ewes in the southeastern United States. Vet Parasitol 109: Li, Y., Miller, J.E., and Franke, D.E., Epidemiological observation and heterosis of gastrointestinal nematode infection in Suffolk, Gulf Coast Native and crossbred lambs. Vet Parasitol 98:

12 Assoc Prof David Piedrafita Head: Parasite Immunology School of Applied Sciences & Engineering; Biotechnology Research Laboratories, Monash University, Churchill, 3842, Australia Phone: david.piedrafita@monash.edu BRIEF BIOGRAPHY Dr David Piedrafita embarked on a PhD in immunoparasitology within the Department of Primary Industries in Victoria, after winning a competitive National Industry Scholarship. In 1996, he was awarded a Wellcome Trust Fellowship and worked on DNA vaccines at Glasgow University in Scotland for 3 years. David returned to Australia in 1999 to study Helminth vaccines and biology and in 2001 set up his own group working on Fasciola (liver fluke). In 2006, he relocated his group to the Biotechnology Research Laboratories at Monash University, where he continues to work on national and international research programs (including Thailand, Indonesia, Africa and Spain) and heads the Parasitology unit. He routinely hosts scientists and graduate students from overseas countries and supervises several PhD students. He is actively involved in promoting parasitology to the general public through educational activities and has been vice president and president of the Australian Society for Parasitology ( ). He was deputy chair for the 2010 International Congress for Parasitology which was held in Melbourne (ICOPA XII) and hosted over 1,750 delegates from around the world. Parasite immunology, Vaccine development and performance and the genetic control of disease resistance are his main research interests. SELECTED PUBLICATIONS - M.S. Lees, N.A. Robinson, A.B. Ingham, A.C. Kotze, D.M. Piedrafita. (2011). Dual oxidase 2 and glutathione peroxidase gene expression are elevated in hyperimmunised sheep challenged with Haemonchus contortus. Veterinary Parasitology (in press). - Jorge F. González, Álvaro Hernández, José M. Molina, Antonio Fernández, Herman Raadsma, Els N.T. Meeusen and Piedrafita, D. (2011). Fecundity in adult Haemonchus contortus parasites is correlated with abomasal tissue eosinophils and gamma-delta T cells in resistant Canaria Hair Breed sheep. Veterinary Parasitology 178: J. Pleasance, H.W. Raadsma, S.E. Estuningsih, E. Meeusen, Piedrafita, D. (2011) Innate and adaptive resistance of Indonesian Thin Tail sheep to liver fluke: A comparative analysis of Fasciola gigantica and F. hepatica infection. Veterinary Parasitology (in press). - Robinson N, Pleasance J, Piedrafita D, Meeusen ENT. (2011). The kinetics of local cytokine and galectin expression after challenge infection with the gastrointestinal nematode, Haemonchus contortus. International Journal for Parasitology 41(5): Piedrafita D, Spithill TW and Raadsma HW (2010). Improving animal and human health through understanding liver fluke immunology. Parasite Immunology 32(8): Hein WR, Pernthaner A, Piedrafita D and Meeusen ENT. (2010). Immune mechanisms of resistance to gastrointestinal nematode infections in sheep. Parasite Immunology 32(8): Piedrafita D, Gonzalez J, Raadsma HW & Meeusen ENT (2010). Increased production through parasite control: can ancient breeds of sheep teach us new lessons? Trends in Parasitology 26(12): Robinson N, Piedrafita D, Snibson K, Harrison P, Meeusen ENT. (2010) Immune cell kinetics in the ovine abomasal mucosa following hyperimmunisation and challenge with Haemonchus contortus. Veterinary Research 41(4):37. - Kemp JM, Robinson NA, Meeusen EN, Piedrafita DM. (2009). The relationship between the rapid rejection of Haemonchus contortus larvae with cells and mediators in abomasal tissues in immune sheep. International Journal for Parasitology. 39(14): Estuningsih, E., Spithill T.W., Raadsma, H.W., Law, R., Adiwinata, G., Meeusen, E. and Piedrafita, D. (2009). Development and application of a faecal antigen diagnostic sandwich ELISA for estimating prevalence of Fasciola gigantica in cattle in central Java, Indonesia. Journal of Parasitology. 95(2),

13 Professor Herman W. Raadsma Director of ReproGen Reprogen-Animal Bioscience Faculty of Veterinary Science Sydney University PM3, Camden, NSW 2570, Australia Phone: herman.raadsma@sydney.edu.au BRIEF BIOGRAPHY Herman joined the University of Sydney, faculty of Veterinary Science as a Research Fellow in Previous employment included 7 years as a Research Officer with NSW Department of Agriculture, Agriculture Research Station Trangie, with responsabilities for sheep breeding research in disease resistance. Postgraduate qualifications obtained from University of New England, Department of Animal Science in applied male reproduction efficiency, and sexpreselection. PhD obtained from the University of Sydney, Department of Animal Health. He was Associate Dean for Research, Faculty of Veterinary Science (1999) and Director of ReproGen from 1999 to date. Primary research responsibilities at the University of Sydney have covered research on genetic aspects of disease resistance in sheep, with a focus on footrot and vaccine responsiveness. More recent research interests cover molecular characterisation of livestock genomes for production and disease resistance genes, with a focus on sheep. Research track record includes acquisition of competitive grant (external) funding (in excess of $6 mil) for core research programs and capital facilities; national and international collaboration with livestock genetics groups; strong record of publication. Strong commitment to animal health and production research, with a special interest in the development and application of genetic principles for control of disease and immune function, and application of DNA technologies in animal breeding programs for superior production. These programs have a focus to enhance sustainable and easy care features of livestock in extensive grazing systems. Contributor to animal health / animal production sciences SELECTED PUBLICATIONS - Pleasance, J., Wiedosari, E., Raadsma, H.W., Meeusen, E., Piedrafita, D. (2011). Resistance to liver fluke infection in natural sheep host is correlated with type-1 cytokine response. Parasite Immunol., 33: Pleasance, J., Raadsma, H.W., Estuningsih, S.E., Widjajanti, S., Meeusen, E., Piedrafita, (2011). Innate and adaptative resistance of Indonesian Thin Tail sheep to liver fluke: a comparative analysis of Fasciola gigantica and Fasciola hepatica infection. Vet. Parasitol., 10: Pryce, J.E., Goddard, M.E., Raadsma, H.W., Hayes, B.J. (2010). Deterministic models of breeding scheme designs that incorporate genomic selection. J. Dairy Sci., 93: Piedrafita, D., Raadsma, H.W., González, J.F., Meeusen, E. (2010). Increased production through parasite control: can ancient breeds of sheep teach us new lessons?. Trends Parasitol., 26: International Sheep Genoma Consortium, Archibald, A.L., Cockett, N.E., Dalrymple, et al (Herman H.W.) (2010). The sheep genome reference sequence: a work in progress. Anim. Genet., 41: Piedrafita, D., Spithill, T.W., Smith, R.E., Raadsma, H.W. (2010). Improving animal and human health trough understanding liver fluke immunology. Parasite Immunol., 32: Raadsma, H.W., Kingsford, N.M., Suharyanta, Spithill, T.W., Piedrafita, D. (2008). Host responses during experimental infection with Fasciola gigantica and Fasciola hepatica in Merino sheep II. Development of a predictive index for Fasciola gigantica worm burden. Vet. Parasitol., 154: Piedrafita, D., Estuningsih, E., Pleasance, J., Prowse, R., Raadsma, H.W., Meeusen, E.N., Spithill, T.W. (2007). Peritoneal lavage cells of Indonesian thin-tail sheep mediate antibody-dependent superoxide radical cytotoxicity in vitro against newly excysted juvenile Fasciola gigantica but no juvenile Fasciola hepatica. Infect Immun, 75: Raadsma, H.W., Kingsford, N.M., Suharyanta, Spithill, T.W., Piedrafita, D. (2007). Host responses during experimental infection with Fasciola gigantica or Fasciola hepatica in Merino sheep I. Comparative immunological and plasma biochemical changes during early infection. Vet. Parasitol., 143: Boscariol, R., Pleasance, J., Piedrafita, D., Raadsma, H.W., Spithill, T.W., (2006). Identification of two allelic forms of ovine CD4 exhibithing a Ser183/Pro183 polymorphism in the coding sequence of domain 3. Vet. Immunol. Immunopathol., 113:

14 Professor Francisco A. Rojo-Vázquez Academic: Real Academia Española de las Ciencias Veterinarias (Royal Spanish Academy of Veterinary Sciences) Head : Anthelmintic resistance and strategic control of helminth in ruminants group Department of Animal Health Faculty of Veterinary University of León Campus de Vegazana, 24071, León, Spain Phone: francisco.rojo@unileon.es BRIEF BIOGRAPHY Professor Rojo-Vázquez obtained the Degree in Veterinary Medicine at the Faculty of Veterinary Medicine of the University of León (Spain) and the PhD in Veterinary Parasitology, Faculty of Veterinary Medicine of León, University of León. He was appointment initially as Assistant Professor of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of León and later on initially as Associate Professor and Full Professor of Parasitology at Faculty of Pharmacy of the University of Salamanca (Spain) where he was also the Dean of the Faculty. Few years later, he moved to the Faculty of Veterinary Medicine of the Universidad Complutense (Madrid) as Professor of Parasitology and Parasitic diseases at the Animal Health Department and finally to the same Department at the Faculty of Veterinary Medicine in the University of León, being also Dean of these institutions. In 2007, he was the Head of the Centro de Investigación en Sanidad Animal (CISA-Centre for Investigation in Animal Health, Spanish Ministry of Science and Innovation) and Subdirector for Research of the National Institute of Agricultural Research (Spanish Ministry of Science and Innovation). Recently he came back to the Veterinary Faculty of the León University. He has also been the President of the Spanish Society of Parasitology and Founding Diplomate of the European Veterinary Parasitology College (EVPC). He is member of the Spanish Academy of Veterinary Science. He is a research collaborator with numerous national and international research centres. He has been principal investigator of several industry and competitive grants. His main research interest is on resistance to anthelmintics, breed resistance against parasites and integrate control of gastrointestinal nematodes and liver fluke infections. SELECTED PUBLICATIONS - Cruz-Rojo, M.A., Martínez-Valladares, M., Álvarez-Sánchez, M.A., Rojo-Vázquez, F.A. (2011). Effect of infection with Teladorsagia circumcincta on milk production and composition in Assaf dairy sheep. Vet. Parasitol., (in press) - Martínez-Valladares, M., Donnan, A., Geldhof, P., Jackson, F., Rojo-Vázquez, F.A., Skuce, P. (2011). Pyrosequencing analysis of the beta-tubulin gene in Spanish Teladorsagia circumcincta field isolates. Vet. Parasitol., (in press) - Martínez-Valladares, M., Cordero-Pérez, C., Castañón-Ordóñez, L., Famularo, M.R., Fernández_pato, N., Rojo-Vázquez, F.A. (2010). Efficacy of a moxidectin/triclabendazole oral formulation against mixed infections of Fasciola hepatica and gastrointestinal nematodes in sheep. Vet. Parasitol., 174: Gutiérrez-Gil, B., Pérez, J., Álvarez, L., Martínez-Valladares, M., de la Fuente, L.F., Bayón, Y., Meana, A., San Primitivo, F., Rojo-Vázquez, F.A., Arranz, J.J. (2009). Quantitative trait loci for resistance to trichostrongylid infection in Churra sheep. Genet. Sel. Evol., 28: Martínez-Valladares, M., Godio-Fernández, R., Vara-del-Río, M.P., Martín, J.F., Rojo-Vázquez, F.A. (2007). Expression of the recombinant protein disulphide isomerise of Teladorsagia circumcincta. Parasite Immunol, 29: Álvarez-Sánchez, M.A., Pérez-García, J., Cruz-Rojo, M.A., Rojo-Vázquez, F.A. (2006). Anthelmintic resistance in trichostrongylid nematodes of sheep farms in Northwest Spain. Parasitol. Res., 99: Martínez-Valladares, M., Vara-del-Río, M.P., Cruz-Rojo, M.A., Rojo-Vázquez, F.A. (2005). Effect of a low protein diet on the resistance of Churra sheep to Teladorsagia circumcincta. Parasite Immunol., 27: Martínez-Valladares, M., Vara-del-Río, M.P., Cruz-Rojo, M.A., Rojo-Vázquez, F.A. (2005). Genetic resistance to Teladorsagia circumcincta: IgA and parameters at slaughter in Churra sheep. Parasite Immunol., 27: Álvarez-Sánchez, M.A., Pérez-García, J., Cruz-Rojo, M.A., Rojo-Vázquez, F.A. (2005). Real Time PCR for the diagnosis of benzimidazole resistance in trichostrongylids of sheep. Vet. Parasitol., 129: Álvarez-Sánchez, M.A., Pérez-García, J., Bartley, D., Jackson, F., Rojo-Vázquez, F.A. (2005). The larval feeding inhibition assay for the diagnosis of nematode anthelmintic resistance. Exp. Parasitol., 110:

15 Professor Michael Stear Professor of Immunogenetics Associate Academic (School of Veterinary Medicine) Head: Veterinary Genes and Protein Group Institute of Infections, Immunity and Inflamation, School of Veterinary Medicine, University of Glasgow, Garscube Campus, Bearsden Road, Glasgow G61 1QH Phone: +44 (0) BRIEF BIOGRAPHY His major interest is in understanding and controlling nematode infections. Most of his research is on Teladorsagia circumcincta infections of sheep. This host-parasite association was chosen deliberately because it is an economically-important infection of sheep. Most advances in our understanding of host-parasite relationships have come from a detailed analysis of deliberate infections or mathematical modelling of natural infections. Both strategies can be used to study parasites of sheep. The plan is to develop a coherent explanation that covers all levels from populations to molecules. Among the achievements are the use of variance analysis to identify and quantify the major components of resistance, the demonstration that many components including the heritability and sex differences vary with age, the finding that a major mechanism of resistance in growing lambs is the mucosal IgA response against fourth-stage larvae which is associated with reduced worm growth and fecundity and the identification of two quantitative trait loci (QTL) for parasite resistance; one in the mhc class II region and one in or around the interferon gamma gene. His current research aims to identify the parasite molecules recognised by resistant but not susceptible hosts. He is the principal investigator of many competitive and private supported grants. SELECTED PUBLICATIONS - Singleton, D.R., Stear, M.J. and Matthews, L. (2011) A mechanistic model of developing immunity to Teladorsagia circumcincta infection in lambs. Parasitology, 138 (3). pp ISSN Ballingall, K.T., Herrmann-Hoesing, L., Robinson, J., Marsh, S. G. E. and Stear, M.J. (2011) A single nomenclature and associated database for alleles at the major histocompatibility complex class II DRB1 locus of sheep. Tissue Antigens, 77 (6). pp ISSN Singleton, D.R., Stear, M.J. and Matthews, L. (2011) A mechanistic model of developing immunity to Teladorsagia circumcincta infection in lambs. Parasitology, 138 (03). pp ISSN Stear, M.J., Singleton, D. and Matthews, L. (2011) An evolutionary perspective on gastrointestinal nematodes of sheep. Journal of Helminthology, 85 (02). pp ISSN X - Murphy, L., Eckersall, P.D., Bishop, S.C., Pettit, J.J., Huntley, J.F., Burchmore, R.J.S. and Stear, M.J. (2010) Genetic variation among lambs in peripheral IgE activity against the larval stages of Teladorsagia circumcincta. Parasitology, 137 (8). pp ISSN Stear, M. J., Boag, B., Cattadori, I. and Murphy, L. (2009) Genetic variation in resistance to mixed, predominantly Teladorsagia circumcincta nematode infections of sheep: from heritabilities to gene identification. Parasite Immunology, 31 (5). pp ISSN Stear, MJ et al. (2007) Detection of genes with moderate effects on disease resistance using ovine mhc and resistance to nematodes as an example. Veterinary Immunology and Immunopathology, 120. pp Stear, MJ, Doligalska, M and Donskow-Schmelter, K (2007) Alternatives to anthelmintics for the control of nematodes in livestock. Parasitology, 134. pp Davies, G, Stear, MJ, Benothman, M, Abuagob, O, Kerr, A, Mitchell, S and Bishop, SC (2006) Quantitative trait loci associated with parasitic infection in Scottish blackface sheep. Heredity, 96. pp Henderson, NG and Stear, MJ (2006) Eosinophil and IgA responses in sheep infected with Teladorsagia circumcincta. Veterinary Immunology and Immunopathology, 112. pp

16 ACKNOWLEDGMENTS The Committees of the International Workshop on Genetic Resistance to Parasites in Small Ruminants gratefully acknowledge the support of the following institutions and companies and wish to thank them for their contribution: Institutions - University of Las Palmas de Gran Canaria - Instituto Universitario de Sanidad Animal - Faculty of Veterinary - Instituto Nacional de Investigaciones y Tecnología Agraria y Alimentaria (Department of Economy and Competitive, Spanish Government) - European Regional Development Fund (ERDF) - Excmo. Ayuntamiento de Arucas - Colegio Oficial de Veterinarios de Las Palmas 16

17 Partners - Merial - Pfizer - JSP - Agua de Firgas 17

18 SPONSORSHIP OPPORTUNITIES The Committees of the International Workshop on Genetic Resistance to Parasites in Small Ruminants is seeking the support of organisations or companies to provide sponsorship. If you are interested, please, send an to: 18