UKTAG COASTAL WATER ASSESSMENT METHOD BENTHIC INVERTEBRATE FAUNA INVERTEBRATES IN SOFT SEDIMENTS (INFAUNAL QUALITY INDEX (IQI))

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1 UKTAG COASTAL WATER ASSESSMENT METHOD BENTHC NVERTEBRATE FAUNA NVERTEBRATES N SOFT SEDMENTS (NFAUNAL QUALTY NDEX (Q)) by Water Framework Directive - United Kingdom Technical Advisory Group (WFD-UKTAG) Page 1 of 17

2 Publisher: Water Framework Directive - United Kingdom Technical Advisory Group (WFD-UKTAG) SNFFER 25 Greenside Place Edinburgh EH1 3AA Scotland December 2008 SBN: SNFFER 2008 All rights reserved. No part of this document may be reproduced, stored in a retrieval system or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording or otherwise without the prior permission of SNFFER. The views expressed in this document are not necessarily those of SNFFER. ts members, servants or agents accept no liability whatsoever for any loss or damage arising from the interpretation or use of the information, or reliance upon views contained herein. Page 2 of 17

3 HEALTH AND SAFETY STATEMENT WARNNG working in or around water is inherently dangerous; persons using this standard should be familiar with normal laboratory and field practice. This published monitoring system does not purport to address all of the safety problems, if any, associated with its use. t is the responsibility of the user to establish appropriate health and safety practices and to ensure compliance with any national regulatory guidelines. t is also the responsibility of the user if seeking to practise the method outlined here, to gain appropriate permissions for access to water courses and their biological sampling. Page 3 of 17

4 1. NTRODUCTON This method statement describes a monitoring system for monitoring, assessing and classifying coastal waters in accordance with the requirements of Article 8; Section 1.3 of Annex ; and Annex V of the Water Framework Directive (2000/60/EC) Geographic application of the method The method can be applied to the coastal waters in England, Northern reland, Scotland and Wales in parts of those waters that are subtidal and have a bed composed of fine muddy sand to mud Quality element assessed by the method The method enables an assessment of the condition of the quality element, "benthic invertebrate fauna", listed in Tables and of Annex V to the Water Framework Directive Pressures to which the method is known to be sensitive The method has been designed to detect the impact on the quality element of general disturbance, particularly of organic enrichment and toxins. 1.4 Parameters used to assess the quality element The method uses a multi-parameter index, nfaunal Quality ndex (Q) for the purpose of assessing the condition of the quality element. The Q is based on three parameters: (i) number of taxa; (ii) AZT* Marine Biotic ndex (AMB); and (iii) Simpson s Evenness. 2. SAMPLNG AND ANALYSS 2.1. Sampling method Within this method, sampling means the collection for identification of samples of benthic invertebrate fauna at a site for the purpose of estimating the ecological quality ratio at that site on the occasion of the sampling Sampling Sites Sampling sites should be in sub-tidal areas consisting of fine muddy sand to mud Method Sampling should comply with the provisions of SO 16665:2005, which gives guidelines for the Page 4 of 17

5 quantitative collection and processing of sub-tidal, soft-bottom macro-faunal samples in coastal waters and specifies development of the sampling programme; requirements for sampling equipment; sampling and sample treatment in the field Analytical method Samples should be sorted, and macro-invertebrate fauna taken from the samples should be identified and counted to create a site taxa and abundance list. The taxa eligible for inclusion in the site taxa list should be determined according to a standard contemporary list of taxa eligible to be unambiguously identified as invertebrate benthic fauna. The analysis of the samples should comply with the provisions of SO 16665:2005, which provides guidelines on sorting, species identification, and storage of collected and processed material. 3. PROCEDURE FOR DERVNG THE ECOLOGCAL QUALTY RATO FOR THE PARAMETERS 3.1. Calculation of the observed value for each parameter Number of taxa The observed value for the parameter, number of taxa, should be the number of taxa listed and identified as present at the site AZT Marine Biotic ndex (AMB) The observed value for the parameter, AZT Marine Biotic ndex (AMB), should be calculated according to the following steps: (i) (ii) (iii) (iv) Each taxon listed in columns 1,3,5,7 or 9 of Table 1 and identified as being present at the site should be attributed to its corresponding ecological group (,,, V, V) in Column 2,4,6,8 or 10 of that Table. The abundance of taxa in each group ("g", "g", "g", "gv" and "gv") should be estimated as the number of specimens of taxa in that group per sample. The relative abundance ("G", "G", "G", "GV", "GV") of each group should be calculated using the equation: G# = g# (g + g + g + gv + gv) where: "#" varies through,,, V, V. (v) The observed value of the AZT Marine Biotic ndex (AMB) should be calculated using the equation: Page 5 of 17

6 Observed value of AMB = [(0 x G) + (1.5 x G) + (3 x G) + (4.5 x GV) + (6 x GV)] Simpson s Evenness The observed value of the parameter, Simpson s Evenness (λ ), of a sample should be calculated by the method of Simpson (1949) using the equation: Observed value of Simpson s evenness = s n p x (n p -1) p = 1 N x (N 1) where "N" is the total number of individual invertebrates identified as present in the sample; "S" is the number of taxa identified as present in the sample; "n p " is the number of individual invertebrates of taxon "p" identified as present in the sample; "p" represents a taxon identified as present in the sample; "p" varies through values of 1 to "S" Calculation of the reference values for each parameter Reference conditions were derived using a combination of best available data and expert judgement. Reference conditions are habitat specific and defined for the surface area sampled and the sieve mesh used for sample processing. The following reference conditions relate to fine muddy sands to muds, with a sample area of 0.1m 2 processed through a 1 mm sieve Number of taxa The value for the parameter, number of taxa, in the reference conditions (S ref ) applicable to areas of coastal waters with subtidal beds composed of fine muddy sand to mud for samples of 0.1m 2, processed through a 1mm sieve, should be taken as: S ref = AZT Marine Biotic ndex The value for the parameter, AZT Marine Biotic ndex, in the reference conditions (AMB ref ) applicable to areas of coastal waters with subtidal beds composed of fine muddy sand to mud for samples of 0.1m 2, processed through a 1mm sieve, should be taken from: (1-AMB ref 7) = Simpson s Evenness Page 6 of 17

7 The value for the parameter, Simpson's Evenness, in the reference conditions (λ' ref ) applicable to areas of coastal waters with subtidal beds composed of fine muddy sand to mud for samples of 0.1m 2, processed through a 1mm sieve, should be taken from: (1-λ' ref ) = Calculation of the ecological quality ratio for the combined parameters The ecological quality ratio for the combined parameters, EQR Q should be calculated using the following equation: EQR Q = [ 0.38 (1 - AMB 7) (1 AMB ref 7) (1-λ ) (1-λ ref ) (S S ref ) ] DEFNTON OF TERMS * AZT: Technological nstitute for Fisheries and Food (AZT), San Sebastian, Spain Page 7 of 17

8 Table 1: List of benthic invertebrate taxa and associated ecological group Column 1 Column 2 Column 3 Column 4 Column 5 Column 6 Column 7 Column 8 Column 9 Taxa Ecological Group Taxa Ecological Group Taxa Ecological Group Taxa Ecological Group Abarenicola claparedi Angulus tenuis Bela powisiana Chone infundibuliformis Diastylis rugosa Abarenicola sp. Anomia ephippium Bela sp. Chone sp. Diastylis sp. Abissoninoe hibernica Anoplodactylus petiolatus N.A Bittium reticulatum Chthamalus stellatus Diastylis tumida Abra alba Anoplodactylus pygmaeus N.A Boccardia chilensis Circe minima Diodora apertura Abra nitida Antenella sp. Boccardia polybranchia Circulus striatus Diogenes pugilator Abra prismatica Anthozoa sp. Boccardia sp. Cirratulus chrysoderma V Diopatra neapolitana Abra sp. Anthura gracilis Bodotria arenosa Cirratulus cirratus V Diplocirrus glaucus Abra tenuis Aonides oxycephala Bodotria scorpioides Cirriformia tentaculata V Dispio uncinata Acanthocardia aculeata Aora gracilis Brada villosa N.A Cirripedo sp. Divaricella divaricata Acanthocardia echinata Aora typica Branchiomma vesiculosum Clausinella fasciata Donax trunculus Acanthocardia paucicostata Aphelochatea multibranchiis N.A Branchiostoma lanceolata Clavidae Doris sp. Acanthocardia sp. Apherusa cirrus Brania oculata Clymene cf. praetermisa Dosinia exoleta Acanthocardia tuberculata Apherusa ovalipes Brania pusilla Clymene lumbricoides Dosinia juv. ndet. Acanthochitona critinus Aphonupis grubei Bugula sp. Clymene modesta Dosinia lupinus Acanthochitona fascicularis Aphrodite aculeata N.A Callianassa sp. Clymene oerstedii Dosinia sp. Achelia hispida Apicularia guerini Callianassa subterranea Clytia sp. Drilonereis fillum Achelia simplex Apistobranchus tullbergi Callianassa truncata Cnidaria sp. Ebalia sp. N.A Aclis gulsonae N.A Aponuphis bilineata Calliostoma papillosum Cochlodesma praetenue N.A Ebalia tuberosa N.A Acronida brachiata Aporrhais pespelecani Calliostoma zizyphinum Copepoda indet. N.A Echinocardium cordatum Acteon sp. Aporrhais sp. Calyptraea sinensis Copepodo sp. N.A Echinocyamus pusillus Actinia equina Apseudes latreillei Campylaspis glabra N.A Corbula gibba Echinoidea sp. Aglaophamus rubella N.A Arcturella sp. N.A Capitella capitata V Corophium acherusicum Echiuroidea sp. Aglaophamus sp. N.A Arenicola marina N.A Capitella sp. V Corophium acutum Echiurus echiurus Aiptasia mutabilis N.A Aricia latreilli Capitellides giardi V Corophium arenarium Edwardsia sp. Taxa Column 10 Group Page 8 of 17

9 Alcyonacea indet N.A Aricidea catherinae Capitomastus minimus V Corophium insidiosum Ehlersia ferrugina N.A Alkmaria romijni Aricidea cerruti Caprella fretensis N.A Corophium multisetosum Ensis sp. Alpheus glaber N.A Aricidea cf. assimilis Caprella linearis N.A Corophium sp. Eocuma dimorpha Alvania crassa Aricidea fragilis Caprella penantis N.A Corophium volutator Eocuma dollfusi Alvania semistriata Aricidea jereysii Carcinus maenas Coryne pusilla Epilepton clarkiae Alvania sp. Aricidea minuta Caryophyllia smithi Corystes cassivelaunus Epithonium clathrus Amatea trilobata Aricidea sp. Caulleriella alata Cossura longocirrata N.A Epitonium turtoni Amathia pruvot Armandia cirrosa Caulleriella bioculata Cossura pygodactylata N.A Ericthonius brasilensis N.A Ampelisca brevicornis Armandia spp Caulleriella sp. Cossura sp. N.A Eteone longa Ampelisca cf. spooneri Aspidosiphon muelleri Caulleriella zetlandica Crangon allmani Eteone picta Ampelisca heterodactyla Astacilla longicornis Cavernularia pusilla Crangon crangon Euclymede praetermissa Ampelisca juv. ndet. Astarte sp. Ceradocus semiserratus Crassostrea angulata Euclymene anis Ampelisca sarsi Astarte sulcata Cerastoderma edule Crepidula fornicata Euclymene oerstedii Ampelisca sp. Astarte triangularis Cerastoderma lamarcki Cucumaria elongata Euclymene sp. Ampelisca spinifer Asterina gibosa Ceratostoma erinaceum Cucumaria sp. Euclymenidae indet. Ampelisca spinimana Astropecten irregularis Cerebratulus marginatus Cultellus pellucidus Eudorella truncatula N.A Ampelisca tenuicornis Astropecten irregularis typicus Cerebratulus sp. Cumopsis fagei Eulalia bilineata Ampelisca toulemonti Athanas nitescens Cereus pedunculatus Cumopsis sp. Eulalia mustela Ampharete filnmarchica Athecata sp. Ceriantario sp. Cyathura carinata Eulalia sanguinea Ampharete grubei Atylus falcatus Cerianthus lloydii Cyclope neritea Eulalia sp. Ampharete juv. ndet. Atylus guttatus Cerianthus membranaceus Cylichna cylindracea Eulalia tripunctata Ampharete lindstroemi Atylus sp. Cerianthus sp. Cylichna sp. Eulalia viridis Ampharete sp. Atylus swammerdami Cestopagurus timidus Cylichnina subcylindrica Eulimella acicuta Amphicteis gunneri Atylus vedlomensis Chaetopterus variopedatus Cymodoce truncata Eulimella sp. Amphipholis squamata Audouinia tentaculata V Chaetozone B spp V Cythara attenuata Eumida bahusiensis Amphitrite johnstoni Autolytus longeferiens N.A Chaetozone cf. gibber V Cythara costata Eumida sanguinea Amphiura brachiata Autolytus sp. N.A Chaetozone gibber V Dardanus arrosor N.A Eumida sp. Amphiura chiajei Axionice maculata N.A Chaetozone setosa V Demonax sp. N.A Eunice harassii Amphiura filiformis Bachycuma brevicornis N.A Chaetozone sp. V Demospongia sp. Eunice sp. Amphiura juv.indet. Balcis alba Chamelea gallina Dentalium novemcostatum Eunice vittata Page 9 of 17

10 Amphiura sp. Barleeia rubra Chamelea gallina striatula Dentalium sp. Euphrosyne foliosa Anaitides lineata Bathyporeia elegans Chamelea striatula Desdemona cf. ornata Eurydice anis Anaitides maculata Bathyporeia nana Chauvetia brunnea Desdemona ornata Eurydice pulchra Anaitides mucosa Bathyporeia pelagica Cheirocratus sundevallii Devonia perrieri Eurydice sp. Anaitides sp. Bathyporeia pilosa Cheriocratus sp. Diastylis bradyi Eurydice spinigera Anapagurus hyndmani Bathyporeia sarsi Chironomida V Diastylis cf. tumida Eurynome aspera Anapagurus laevis Bathyporeia sp. Chlamys varia Diastylis cornuta Eurynome spinosa Anapagurus sp. Bathyporeia tenuipes Chone collaris Diastylis laevis Eurysyllis tuberculata Anguilla anguilla Bela nebula Chone fillicaudata Diastylis lucifera Exogone naidina Exogone sp. Hesionura elongata Leucothoe spinicarpa Marphysa fallax Nephtys cirrosa Fabricia sabella N.A Heterocirrus bioculatus V Levinsenia gracilis N.A Marphysa sanginea Nephtys hombergi Fabulina fabula Heterocirrus spp V Lilleborjia pallida Marphysa sp. Nephtys hystricis Filograna implexa N.A Heteromastus filiformis Liocarcinus arcuatus Marphysa sp. (belli?) Nephtys incisa Galathea intermedia Hexacorallia sp. Liocarcinus arcuatus Martastherias glacialis Nephtys juv. Spp Galathea sp. Hiatella artica Liocarcinus depurator Mediomastus fragilis Nephtys kersivalensis Galathea squamifera Hinia incrassata Liocarcinus holsatus Megaluropus agilis Nephtys paradoxa Galathowenia oculata Hinia juv. indet. Liocarcinus marmoreus Megamphopus cornutus Nephtys sp. Galeomna turtoni Hinia pygmaea Liocarcinus pusillus Melinna cristata Nephtys sp. juv. Gammarella fucicola Hinia reticulata Liocarcinus sp. Melinna palmata Nephtys spp Gammaridea Hippolyte varians Liocarcinus vernalis Melinna sp. punctata Gammaropsis palmata Hippomedon denticulatus Liocarcinus zariquieyi Melita gladiosa Nereiphylla rubiginosa N.A Gammaropsis shophiae Hyala vitrea Listriella picta Melita palmata Nereis caudata V Gammaropsis sp. Hyale nilssoni Loripes lucinalis Merceriella enigmatica Nereis cf. lamellosa Gammarus insensibilis Hyalinoecia bilineata N.A Lucinoma borealis Metaphoxus fultoni Nereis diversicolor Gammarus sp. Hyalinoecia fauveli N.A Lucinoma borealis Metaphoxus pectinatus Nereis longissima Gari costulata Hyalinoecia sp. N.A Lumbrinerides sp. Metazoea de porcellanidae N.A Nereis sp. Gari depressa Hydrobia ulvae Lumbrineriopsis paradoxa Microdeutopus anomalus Nothria geofilliformis Gari fervensis Hydroides norvegica N.A Lumbrineris cf. gracilis Microdeutopus damnoniensis Nothria lepta Gari tellinella dotea linearis N.A Lumbrineris emandibulata Microdeutopus sp. Nothria sp. Page 10 of 17

11 Gariidae indet. dunella picta N.A Lumbrineris emandibulata mabiti Microdeutopus stationis Notirus irus Gattyana cirrosa N.A nachus dorsettensis Lumbrineris gracilis Microdeutopus versiculatus Notocirrus sp. N.A Gibbula magus nachus sp. larva Lumbrineris impatiens Microphthalmus sczelkowii N.A Notomastus latericeus Glycera alba phimedia obesa Lumbrineris latreilli Microspio sp. N.A Notomastus lineatus Glycera capitata phinoe serrata Lumbrineris latreilli Micrura sp. N.A Notomastus sp. Glycera convoluta phinoe sp. Lumbrineris sp. Modiolula phaseolina Nucula nitida Glycera lapidum Jaera albifrons Lunatia alderi Modiolus barbatus Nucula nitidosa Glycera rouxii Jaera sp. Lutraria angustior Modiolus gallicus Nucula nucleus Glycera sp. Janira maculosa N.A Lutraria lutraria Modiolus modiolus Nucula sp. Glycera tesselata Jasmineira elegans N.A Lutraria lutraria Monoculodes carinatus Nucula sulcata Glycera tridactyla Jupiteria minuta N.A Lutraria sp. Monopylephorus irroratus V Nucula turgida Glycera unicornis Kefersteinia cirrata N.A Lyonsia norvegicum Montacuta ferruginosa Ocenebra erinacea Glycinde nordmani Kellia suborbicularis Lysianassa ceratina Musculus discors Odostomia sp. Glycinde nordmanni Labidoplax cf. thomsoni Lysianassa insperata Mya arenaria Oligochaeta V Gnathia oxyurea Labidoplax digitata Lysidice ninetta Myrtea spinifera Onuphidae juvenil Gobius niger Labidoplax spp Lysippe labiata N.A Mysella bidentata Onuphis cf. geophiliformis Goniada maculata Lacydonia miranda N.A Macoma baltica Mysia undata Onuphis conchylega Goniada sp. Lagisca extenuata Macropodia rostrata Mysidacea Onuphis eremita Goodallia triangularis Lanice cirrata Mactra corallina Mystides elongata Ophelia bicornis Gouldia minima Lanice cirrata Mactra stultorum Mystides limbata Ophelina acuminata N.A Gregariella barbatella Lanice conchilega Mactracea indet. Mytilaster minimun Ophiocentrus brachiatus Guernea coalita N.A Lanice conchilega Maera grossimana Mytilus edulis Ophiocomina nigra Gymnammodytes semisquamatus N.A Lanice spp Maera othonis Nassarius incrassatus Ophiodromus exuosus Gyptis capensis Laonice sp. N.A Maera sp. Nassarius reticulatus Ophiopsila aranea Gyptis rosea Leanira yhleni N.A Magelona alleni Natantia sp. N.A Ophiotrix fragilis Halcampa sp. Lembos sp. N.A Magelona filliformis Natica alderi Ophiura albida Haminoea navicula Lepidonotus clava Magelona minuta Natica catena Ophiura ophiura Harmothoe antilopes Lepidonotus squamatus Magelona mirabilis Neanthes caudata Ophiura sp. Harmothoe cf. lunulata Leptocheirus pectinatus Magelona papillicornis Neanthes irrorata Ophiura texturata Page 11 of 17

12 Harmothoe glabra Leptocheirus pilosus Magelona sp. Neanthes juv. indet. Ophiura texturata juv. Harmothoe imbricata Leptochelia savignyi N.A Magelona wilsoni Neanthes sp. Ophryotrocha labronica Harmothoe impar Leptochiton asellus Malacoceros ciliata Nebalia bipes V Ophryotrocha puerilis Harmothoe lunulata Leptochiton cancellatus Malacoceros fuliginosus V Nebalia sp. indet. V Ophryotrocha sp. Harmothoe sp. Leptochiton inhaerens Malacoceros girardi Nebalia thyphlops Opistodonta pterochaeta N.A Harmothoe sp.(antilopes?) Leptoneris glauca Malacoceros sp. Nematoda Orbinia cuvieri N.A Harmothoe spinifera Leptosynapta cf. gallienei Malacoceros vulgaris Nematonereis unicornis Orchomene nana N.A Harpinia antennaria Leptosynapta gallienei Maldane glebifex Nemertea Orchomene similis N.A Harpinia pectinata Leptosynapta inhaerens Manayunkia aestuarina Neoamphitrite anis N.A Oriopsis armandi N.A Harpinia sp. Leucothoe incisa Mangelia attenuata Neoamphitrite cf. affinis N.A Oriopsis sp. N.A Haustorius arenarius Leucothoe incisa Mangelia nebula Neoamphitrite sp. N.A Ostrea edulis Hediste diversicolor Leucothoe lilljeborgi Mangelia smithi Nephtys assimilis Ovatella myosotis N.A Hermione hystrix Leucothoe richiardii Mangelia sp. Nephtys caeca Owenia filliformis Hesione pantherina Leucothoe sp. Marphysa bellii Nephtys cf. paradoxa Owenia fusiformis Pachygrapsus marmoratus Phyllodoce rosea Protodrilus sp. N.A Sphaerosyllis hystrix Tholarus cranchii Paguroidea indet. Phyllodoce sp. Psamechinus miliaris Sphaerosyllis pyrifera Thracia phaseolina Pagurus bernhardus Phylocheras bispinosus Psammobia costulata Sphaerosyllis sp. Thracia villosiuscula Pagurus prideauxi Phylocheras monacantus Psammolyce arenosa Sphaerosyllis taylori Thyasira exuosa Pagurus sp. larva Phylocheras trispinosus Pseudobrania sp. Sphenia binghami Thyone fusus Palaemon serratus Pilargis verrucosa Pseudocuma longicornis Spio armata Timoclea ovata Pandora albida Pilumnus hirtellus Pseudocuma similis Spio decoratus Tonicella marmorea Pandora inaequivalvis Pinnotheres pisum N.A Pseudocuma sp. Spio fillicornis Tricolia pullus Pandora pinna Pionosyllis serrata Pseudopolydora antennata V Spio martinensis Triphora adversa Panoploea minuta Pisidia longicornis Pseudopolydora paucibranchiata V Spio sp. Triphora aspera Paradoneis armata Pisidium sp. indet. Pseudopolydora pulchra V Spiochaetopterus costarum Triphora perversa Paradoneis lyra Pisione remota Pseudopolydora sp. V Spiochaetopterus sp. Trivia monacha Paragnathia formica Pista cristata Pseudoprotella phasma N.A Spiochaetopterus typicus Trophonopsis muricatus Paramphitrite tetrabranchia N.A Plakosyllis brevipes Pseudosyllis brevipennis Spiophanes bombyx Tryphosella nanoides Paraonis fulgens N.A Plathelmintes Pseudosyllis sp. Spiophanes kroyeri Tryphosella sarsi Page 12 of 17

13 Paraonis gracilis N.A Platynereis dumerilii Pygospio elegans Spirobranchus polytrema N.A Tryphosites longipes Paraonis lyra N.A Pleuronectidae sp. Quadrans serratus Spisula elliptica Tubifilcoides benedii V Parapionosyllis cf. gestans Podoceus variegatus N.A Raphitoma purpurea Spisula solida Tubifilcoides pseudogaster V Parapionosyllis gestans Poecilochaetus serpens Raphitoma sp. Spisula subtruncata Tubulanus polymorphus Parapionosyllis labronica Poecilochaetus serpens Retusa truncatula Staurocephalus rudolphii V Tubulanus spp Parapionosyllis minuta Polycirrus aurantiacus V Retusa umbilicata Stenothoe monoculoides Turbelario sp. N.A Parapionosyllis sp. Polycirrus cf. medusa V Rhizorus acuminatus N.A Stenothoidae Turboella parva Parathelepus sp. Polycirrus medusa V Ringicula auriculata Sternaspis scutata Turbonilla acuta Pariambus typicus Polycirrus sp. V Ringicula conformis Sthenelais boa Turbonilla elegantissima Pariambus typicus varinermis Polycirrus tenuisetis V Ringicula sp. Sthenelais cf. minor Turbonilla lactea Parvicardium exiguum Polydora antennata V Sabella pavonina Sthenelais limicola Turbonilla rufa Parvicardium minimum Polydora caeca V Sabella sp. Sthenelais minor Turbonilla spp Parvicardium ovale Polydora ciliata V Sabellaria alveolata Sthenelais sp. Turritella communis Parvicardium papillosum Polydora ava V Sabellaria spinulosa Streblosoma bairdi N.A Turritella triplicata Parvicardium scabrum Polydora juv. spp V Scalibregma in atum Streblospio dekhuyzeni Unciola crenatipalma N.A Pectinaria auricoma Polydora ligerica V Scaphander lignarius Streblospio shrubsolii Upogebia cf. tipica Pectinaria koreni Polydora ligni V Schistomeringos caeca Stremblosoma intestinalis N.A Upogebia deltaura Pectinaria sp. Polydora polybranchia V Schistomeringos rudolphi V Striarca lactea Upogebia pusilla Perinereis cultrifera Polydora pulchra V Scionella lornensis N.A Sycon ciliatum Upogebia sp. Perioculodes longimanus Polydora sp. V Sclerocheilus minutus N.A Sycon raphanus Upogebia stellata Pharus legumen Polygordius apendiculatus Scolaricia sp. Syllis cornuta Upogebia typica Phascolion strombi Polymnia nebulosa Scolaricia typica Syllis gerlachi Urothoe brevicornis Phascolion strombus Polynoe scolopendrina Scolelepis fuliginosa V Syllis gracilis Urothoe elegans Phascolosoma elongatum Polyophthalmus pictus Scolelepis sp. Syllis prolifera Urothoe poseidonis Phascolosoma granulatum Pomatoceros lamarckii N.A Scolelepis squamata Syllis sp. Urothoe pulchella Phascolosoma vulgare Pomatoceros triqueter N.A Scoloplos armiger Syllis variegata Vauthompsonia cristata N.A Phaxas pellucidus Pontocrates altamarinus Scrobicularia plana Synchelidium haplocheles Veneracea Pherusa monilifera Pontocrates arenarius Scrupocellaria scrupea N.A Synchelidium maculatum Venerupis aurea Pherusa plumosa Portumnus latipes Semivermilia sp. N.A Tanais dulongii N.A Venerupis pullastra Page 13 of 17

14 Pherusa sp. Potamilla reniformis N.A Sertulariidae Tapes decussata Venerupis rhomboides Philine aperta Potamilla sp. N.A Sextonia longirostris Telepsavus costarum Venerupis senegalensis Philine loweni Potamilla torelli N.A Sigalion cf. mathildae Telina tenuis Venus casina Philine spp Potamopyrgus jenkinsi Sigalion mathildae Tellimya ferruginosa Venus fasciata Pholoe minuta Praxillea oerstedi N.A Sigalion squamatum Tellina compressa Venus gallina Pholoe synopthalmica Prionospio caspersi V Siphonoecetes kroyeranus Tellina donacina Venus juv. sp. Phoronis psammophila Prionospio cirrifera V Siphonoecetes sp. Tellina fabula Venus ovata Photis longicaudata Prionospio ehlersi V Siphonoecetes striatus Tellina pusilla Venus striatula Phoxocephalus rudolphii Prionospio fallax V Sipuncula Tellina pygmea Venus verrucosa Phthisica marina Prionospio malmgreni V Skenea sp. Tellina sp. Veretillum cynomorium Phyllochaetopterus sp. N.A Prionospio multibranchiata V Skenia serpuloides N.A Tellina squalida Veretillum sp. Phyllodoce groelandica Prionospio sp. V Socarnes erythrophthalmus N.A Tellina tenuis Verruca stromia Phyllodoce lamelligera Prionospio steenstrupi V Solen marginatus Terebella lapidaria Vituperis esribosa V Phyllodoce laminosa Processa canaliculata Solenacea Terebellides stroemi Xantho pilipes N.A Phyllodoce lineata Processa modica Sphaerodoropsis sp. N.A Terebellomorpha sp. indet Xenosyllis scabra Phyllodoce longipes Processa nouveli Sphaeroma monodi Thalassema neptuni Zenobiana prismatica Phyllodoce maculata Processa parva Sphaeroma rugicaudata Tharyx marioni N.A Zoantharia sp. Phyllodoce mucosa Processa sp. Sphaeroma serratum Thelepus setosus N.A Phyllodoce paretti Protodorvillea kefersteini Sphaerosyllis bulbosa Thia scutellata Page 14 of 17

15 Annex A WORKED EXAMPLE A site was sampled with the following results: A.1 NUMBER OF TAXA (S) The number (S) of taxa in the site species list was 30. A.2 AZT MARNE BOTC NDEX (AMB) Species were attributed to the ecological groups as described in Section The sample numbers in each group are shown in the Table below. Group # Abundance g# Relative Abundance G# V g + g + g + gv + gv = 5000 (G + G + G + GV + GV) = 4.17 The observed value of AMB according to was A.3 SMPSON S EVENNESS The number of taxa found (S) was 30. The total number of organisms (N) was The number of organisms found for each taxon is shown in Table 1. Simpson s evenness was calculated in Table 1 according to Table 1: Calculation of Simpson's Evenness Species P n p n p (n p 1) (N (N-1)) Page 15 of 17

16 Totals 5, ( = λ ) A.4 Q n reference conditions, this site would have [1 AMB ref 7] : 0.75 [1-λ ref ] : 0.96 S ref : 35 EQR Q = [0.38 (1 - AMB 7) (1 AMB ref 7) (1-λ ) (1-λ ref ) (S S ref ) ] 0.6 = [0.38 ( ) (1-0.08) (30 35) ] 0.6 = 0.69 Page 16 of 17

17 FURTHER READNG SO (2005) Water quality -- Guidelines for quantitative sampling and sample processing of marine soft-bottom macrofauna, SO Borja, A., Franco, J., Pérez, V., (2000). A marine biotic index to establish the ecological quality of soft-bottom benthos within European Estuarine and Coastal environments. Marine Pollution Bulletin 40 (12), Simpson, E.H. (1949) Measurement of diversity. Nature 163, 688 Borja, A., Josefson, A. B., Miles, A., Muxika,., Olsgard, F., Phillips, G., Rodríguez, J. G., and Rygg, B. (2007). An approach to the intercalibration of benthic ecological status assessment in the North Atlantic ecoregion, according to the European Water Framework Directive. Marine Pollution Bulletin 55 (2007), Page 17 of 17