Analysis of Antibiotic/ Drug Residues in Food Products of Animal Origin

Analysis of Antibiotic/ Drug Residues in Food Products of Animal Origin

Analysis of Antibiotic/Drug Residues in Food Products of Animal Origin Edited by Vipin K. Agarwal The Conneclicut Agricullu1'lll Expen"ment Skltion New HlWen, Connecticui Springer Science+ Business Media, LLe

Llbrary of Congress Cataloglng-In-Publlcatlon Data Analysls of antibiotic/drug residues In fooa products of anl.al origin edlted by Vlpln K. Agarwa'. p. c. Proceedlngs of an A.erican Che.lcal Soclety Agrlcultural and food Che.lstry Dlvlslon Sy.pOSiUN on Antibiotic/Drug Resldues In Food Products of Anl.al Orlgln, held August 25-30, 1991, In New York, New York"--T.p. verso. Includes blbllographlcal references and Index. ISBN 978-1-4613-6480-1 ISBN 978-1-4615-3356-6 (ebook) DOI 10.1007/978-1-4615-3356-6 1. Veterlnary drug resldues--analysls--congresses. 2. Antibiotic resldues--analysls--congresses. 1. Agarwal, Vlpln K., 1955- II. American Chemlcal Soclety. Divlsion of Agrlcultural and Food Che.lstry. III, A.erlcan Chemlcal Soclety Agricultural and Food Che.lstry Dlvlslon Sy.posium on Antibiotic/Drug Resldues In Food Products of Anl.al Origln (1991, New York, N.Y.l IV. Title, Analysls of anltblotic drug residues in food products of animal orlgln. [DNLM, 1. Antlblotics--analysls--congresses. 2. Drug Residues -analysls--congresses. 3. Food Contamination--congresses. 4. Meat- -analysls--congresses. HA 701 A352 19911 RA1270.V47A53 1992 SS4'.07--dc20 DNLM/DLC for Llbrary of Congress 92-12153 CIP Proceedings of an American Chemical Society Agricultural and Food Chemistry Division Symposiwn on AntibioticlDrug Residues in Food ProductS of Animal Origin" held August 25--30, 1991, in New York, New York ISBN 978-1-4613-6480-1 1992 Springer Science+Business Media New York OriginaIly published by Plenwn Press New York in 1992 Softcover reprint of the hardcover Ist edition Ali rights reserved No pan of this book may be reproduced, stored in a retrieval system, or transmitted in any foem or by any means, elecuonic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from the Publisher

PREFACE In the last three decades. use of antibiotics/drugs in animal husbandry programs has grown tremendously. Antibiotics/drugs are used therapeutically to cure diseases. and subtherapeutically to control the outbreak of diseases, improve feed efficiency and promote growth. The presence of antibiotic/drug residues in food products of animal origin. i.e meat, poultry and milk, can be a potential health hazard to consumers. Significant research is being done to develop new methods or to improve on existing methods to confirm and quantitatively determine the antibiotic/drug residues in meat, poultry and milk. This book covers recent development and application of various analytical techniques for the determination of antibiotic/drug residues in food products of animal origin. I thank the authors for their time and efforts in preparing the manuscripts and "all the reviewers for reviewing the manuscripts. I also thank to the Division of Agricultural and Food Chemistry of the American Chemical Society for sponsoring the symposium and Hewlett Packard, Palo Alto, CA, Perkin Elmer Corp., Norwalk, CT, Millipore Corporation, Milford, MA, and Kraft General Foods, Glenview, IL, for their generous financial support for the symposium. Vipin K. Agarwal New Haven, CT v

CONTENTS Importance of Laboratory Validations and Accurate Descriptions of Analytical Procedures for Drug Residues in Foods.... C. J. Barnes Incidence of Residues in Foods of Animal Origin... 5 M. S. Brady and S. E. Katz Radioimmunoassay of Antibiotic/Drug 2 Residues in Foods of Animal Origin... 23 S. J. Steiner and J. N. Harris Current Problems Associated with the Detection of Antibiotic/Drug Residues in Milk and Other Food... 31 S. E. Charm ELISA and Its Application for Residue Analysis of Antibiotics and Drugs in Products of Animal Origin... 57 D. E. Dixon-Holland Evaluation and Testing of Charm Test II Receptor Assays for the Detection of Antimicrobial Residues in Meat... 75 G. O. Korsrud, C. D. C. Salisbury, A. C. E. Fesser and J. D. MacNeil Immunochemical Methods In the Analysis of Veterinary Drug Residues... 81 N. Haagsma and C. van de Water Residues of Anabolic Steroids in Muscle Tissues from Carcasses with Positive Injection Sites... 99 C. H. Van Peteghem, E. Daeseleire and A. De Guesquiere Approaches to the Detection and Confirmation of Drug Residues in Milk... 107 M. C. Carson, D. N. Heller, P. J. Kijak, and M. H. Thomas

The Application of Matrix Solid Phase Dispersion (MSPD) to the Extraction and Subsequent Analysis of Drug Residues in Animal Tissues... 119 S. A. Barker and A. R. Long Liquid Chromatographic Approaches to Determination of B-Lactam Antibiotic Residues in Milk and Tissues... 133 W. A. Moats Comparison of Chromatographic Procedures for Determining Residues of Penicillins... 147 M. Petz A High Performance Liquid Chromatographic Method for the Determination of Nine Sulfonamides in Mi lk... 165 V. K. Agarwal Sulfadimethoxine and Sulfamonomethoxine Residue Studies in Chicken Tissues and Eggs... 173 T. Nagata, M. Saeki, T. Ida and M. Waki The Current Overview of Feed Additives and Veterinary Drugs and Their Residual Analysis in Japan... 187 H. Nakazawa, M. Fujita and M. Horie Application of Electrochemical and UV/Visible Detection to the LC Separation and Determination of Methylene Blue and Its. Demethylated Metabolites from Milk... 197 J. E. Roybal, R. K. Munns, D. C. Holland, J. A. Hurlbut and A. R. Long The Presence of Dihydroerythroidines in the Milk of Goats Fed Erythrina poeppigiana and E. berterona Fol iage... 211 L. D. Payne and J. P. Foley Analysis of Selected Chemotherapeutics and Ant i parasi tics... 225 R. Malisch Contributors... 253 Index... 259 viii