Australian and New Zealand College of Veterinary Scientists Membership Examination June 2018 Veterinary Pathology Paper 1 Perusal time: Fifteen (15) minutes Time allowed: Two (2) hours after perusal Answer ALL FOUR (4) questions Answer FOUR (4) questions, each worth 30 marks... total 120 marks 2018 Australian and New Zealand College of Veterinary Scientists ABN 00 50 000894 208 This publication is copyright. Other than for the purposes of and subject to the conditions prescribed under the Copyright Act, no part of it may in any form or by any means (electronic, mechanical, microcopying, photocopying, recording or otherwise) be reproduced, stored in a retrieval system or transmitted without prior written permission. Enquiries should be addressed to the Australian and New Zealand College of Veterinary Scientists Veterinary Pathology Paper 1 Page 1 of 3
Paper 1: Veterinary Pathology Answer all four (4) questions 1. Outline the role of mast cells in the development of disease. Your answer should include the mechanisms for mast cell activation and degranulation, and the contents and functions of mast cell granules. (30 marks) 2. Apoptosis is a vital process with many and varied roles within the body. Answer all parts of this question: a) Define apoptosis, and describe some of the processes in which apoptosis is particularly important. (10 marks) b) Outline the pathways of apoptosis. (15 marks) c) Briefly describe the morphological appearance of a cell undergoing apoptosis. (5 marks) 3. Answer two (2) of the following: (for a total of 30 marks) a) Many grading schemes for tumours have been published in the field of veterinary pathology. However, when these schemes are applied, they are often poorly predictive of tumour behaviour. Identify possible reasons for the failure of tumour grading schemes to predict tumour behaviour. (15 marks) b) Define amyloid and outline two (2) examples of amyloidosis in veterinary medicine. Your examples should include the type of amyloid involved. (15 marks) Question 3 continued over page Veterinary Pathology Paper 1 Page 2 of 3
c) Virchow s triad depicts the key initiators of thrombosis: i. List the three (3) components, and for each of the components describe three (3) diseases or alterations within the body that may predispose to thrombosis by altering the component. (10 marks) ii. Describe the expected gross appearance of a thrombus that develops within a large artery. (5 marks) 4. Answer two (2) of the following: (for a total of 30 marks) a) Describe how microbial agents may predispose to the development of autoimmunity. (15 marks) b) Ras is the most frequently mutated oncogene in human cancers. Describe both the normal function of the Ras protein and why mutations within the Ras oncogene can influence the neoplastic transformation of a cell. (15 marks) c) Endogenous pigments are often seen in the examination of tissues, either by cytology or histology. Choose two (2) endogenous pigments and briefly discuss the appearance and significance of each pigment when it is seen in a pathological setting. Include in your answer the most likely organ or tissue affected, as well as a special stain (if applicable) that you may use to highlight or confirm the nature of the pigment. (15 marks) End of paper Veterinary Pathology Paper 1 Page 3 of 3
Australian and New Zealand College of Veterinary Scientists Membership Examination June 2018 Veterinary Pathology Paper 2 Perusal time: Fifteen (15) minutes Time allowed: Two (2) hours after perusal Answer ALL FOUR (4) questions Answer FOUR (4) questions, each worth 30 marks... total 120 marks 2018 Australian and New Zealand College of Veterinary Scientists ABN 00 50 000894 208 This publication is copyright. Other than for the purposes of and subject to the conditions prescribed under the Copyright Act, no part of it may in any form or by any means (electronic, mechanical, microcopying, photocopying, recording or otherwise) be reproduced, stored in a retrieval system or transmitted without prior written permission. Enquiries should be addressed to the Australian and New Zealand College of Veterinary Scientists Veterinary Pathology Paper 2 Page 1 of 8
Paper 2: Veterinary Pathology Answer all four (4) questions 1. Answer two (2) of the following: (for a total of 30 marks) a) Describe the pathogenesis of pemphigoid diseases in dogs. In addition, describe the histological appearance of pemphigus foliaceus. (15 marks) b) Below and on the following page are the haematology, biochemistry and urinalysis results from a ten-year-old, female, spayed Jack Russell terrier. The referring veterinarian reports a two-month history of progressively worsening polyuria, polydipsia, and weight loss, and mild dehydration is noted on clinical examination. (15 marks) i. Provide a brief interpretation these results. ii. iii. Outline the most likely cause(s) of these changes and provide the most probable diagnosis(es). Provide a recommendation to the veterinarian for the diagnostic tests that may yield additional useful information. Haematology Result Units Reference Intervals RBC count 8.8 H x10 12 /L 5.4 8.5 Haemoglobin 215 H g/l 125 201 Haematocrit 64 H L/L 40 60 MCV 68 63 73 MCHC 345 330 360 Platelet count 256 x10 9 /L 177 398 WBC count 33.0 H x10 9 /L 5.3 19.8 Neutrophil ABS 30.0 H 3.1 14.4 Lymphocyte ABS 0.5 L x10 9 /L 0.9 5.5 Monocyte ABS 2.5 H x10 9 /L <1.6 Eosinophil ABS 0.0 x10 9 /L <1.3 Basophil ABS 0.0 x10 9 /L rare Question 1. b) continued over page Veterinary Pathology Paper 2 Page 2 of 8
Biochemistry Result Units Reference Intervals Sodium 130 L mmol/l 142 150 Potassium 3.8 L mmol/l 3.9 5.9 Chloride 101 mmol/l 99 116 Bicarbonate 10.1 L mmol/l 15.2 25.0 Anion gap 27.1 H 14 22 Glucose, serum 23.0 H mmol/l 3.3 6.8 Urea 13.2 H mmol/l 3.0 10.6 Creatinine 178 H µmol/l 54 152 Calcium 2.3 mmol/l 1.6 2.7 Phosphorous 2.6 H mmol/l 0.8 2.4 Protein, total 75 H g/l 48 71 Albumin 42 H g/l 23 39 Globulins 33 g/l 17 39 Bilirubin, total 3.6 µmol/l 0 4.3 ALP 578 H IU/L 20 155 AST 80 H IU/L 17 69 ALT 101 H IU/L 16 91 GGT 3 IU/L 0 9 CK 359 IU/L 73 510 Cholesterol 10.3 H mmol/l 3.3 8.2 Amylase 856 IU/L 317 937 Lipase 457 IU/L 70 950 Total T4 9 L nmol/l 13 52 TSH (endogenous) 0.09 L µg/l 0.1 0.6 Free T4 18.2 nmol/l 15 44 Sample appearance Lipaemic Urinalysis Method: cystocentesis SG 1.023 Protein: 1+ Glucose 3+ Ketones: 1+ Continued over page Veterinary Pathology Paper 2 Page 3 of 8
c) Below and on the following page are the haematological and biochemical results from a 12-month-old Holstein Friesian heifer presenting to the referring veterinarian as recumbent and dehydrated, with increased respiratory effort. (15 marks) i. Provide a brief interpretation these results. ii. Outline the most likely cause(s) of these changes and most probable diagnosis(es). Haematology Result Units Reference Intervals RBC count 7.08 x10 12 /L 4.9 7.5 Haemoglobin 121 H g/l 84 120 Haematocrit 0.339 H L/L 0.21 0.30 MCV 48 fl 36 50 MCH 17 pg 14 19 MCHC 356 g/l 380 430 Machine platelet count 548 x10 9 /L 160 650 Smear platelet comment clumped and adequate White cell count 25.8 H x10 9 /L 5.1 13.3 Neutrophil ABS 23.2 H x10 9 /L 1.7 6.0 Band ABS 0.3 H x10 9 /L <0.2 Lymphocyte ABS 2.3 x10 9 /L 1.8 8.1 Monocyte ABS 0.0 x10 9 /L 0.1 0.7 Eosinophil ABS 0.0 x10 9 /L 0.1 1.2 Basophil ABS 0.0 x10 9 /L <0.2 Fibrinogen 8 H g/l 3 7 PCV 35 % 0.24 0.38 Plasma protein 103 H g/l 60 80 Plasma appearance Smear morphology Normal RBC: normal. WBC: normal. Question 1. c) continued over page Veterinary Pathology Paper 2 Page 4 of 8
Biochemistry Result Units Reference Intervals Sodium 138 mmol/l 136 145 Potassium 3.0 L mmol/l 3.6 5.6 Chloride 59 L mmol/l 94 111 Bicarbonate 56 H mmol/l 20 30 Anion gap 24 H mmol/l 11 19 Glucose, serum 10.8 H mmol/l 2.0 3.0 Urea 21.0 H mmol/l 2.0 6.6 Creatinine 214 H µmol/l 40 165 Calcium 2.28 mmol/l 2.0 3.0 Phosphate 2.7 H mmol/l 1.2 2.3 CA:P ratio 0.8 Protein, total 92 H g/l 59 73 Albumin 38 H g/l 28 36 Globulins 54 H g/l 27 50 Bilirubin, total 12.1 H µmol/l 0 6.8 AST 256 H IU/L 53 105 GLDH 7.6 IU/L 2.0 23 GGT 34 IU/L 20 46 CK 650 H IU/L 82 96 Magnesium 1.10 mmol/l 0.7 1.23 Triglycerides 0.3 mmol/l 0.17 0.51 B-OHB 0.21 mmol/l 0.3 1.0 Sample appearance Normal Continued over page Veterinary Pathology Paper 2 Page 5 of 8
2. Answer three (3) of the following: (for a total of 30 marks) a) Briefly discuss equine arboviral disease in regards to cause and pathogenesis. Include both endemic and important exotic diseases. Include in your answer a summary of the diagnostic tests that are available and a description of the most likely clinical signs in affected horses. (10 marks) b) Answer two (2) of the following sub-questions: (for a total of 10 marks) i. Outline the causative agent, gross lesions and histological lesions of amoebic gill disease. (5 marks) ii. iii. Describe the histologic lesions that enable a diagnosis of death due to calicivirus infection in rabbits. (5 marks) Name the causative agent and describe the gross and histological lesions of spotty liver disease in chickens. (5 marks) c) Discuss the pathogenesis, gross lesions, and histology of Lawsonia intracellularis infection in pigs. (10 marks) d) Answer two (2) of the following sub-questions: (for a total of 10 marks) i. Provide differential diagnoses for eosinophilic pleocytosis from a cerebrospinal fluid (CSF) sample in dogs. (5 marks) ii. iii. Outline the expected clinical pathological findings for neonatal isoerythrolysis in a foal. (5 marks) Discuss the cytologic features of cutaneous follicular tumours and cysts in dogs and cats. (5 marks) e) Outline the cause, pathogenesis, gross lesions, histological lesions and diagnosis of blackleg in cattle. (10 marks) Continued over page Veterinary Pathology Paper 2 Page 6 of 8
3. Answer three (3) of the following: (for a total of 30 marks) a) List the expected findings in two (2) of the following: (for a total of 10 marks) i. Bronchoalveolar lavage (BAL) fluid from a horse with recurrent airway obstruction (RAO). (5 marks) ii. Synovial fluid from a calf with septic polyarthritis. (5 marks) iii. Piroplasmosis in a kangaroo (including a description or drawing of the parasite). (5 marks) b) Outline four (4) possible causes of microcytosis in dogs and how these might be differentiated. (10 marks) c) Discuss the typical clinical presentation, methods of diagnosis, gross appearance, histological appearance and expected behaviour of urothelial carcinomas in dogs. (10 marks) d) Discuss how gross appearance, cell count, cytology and biochemical analysis of feline thoracic fluid might help distinguish between the potential causes of pleural effusion. (10 marks) e) Describe the gross appearance of the heart and the probable sequelae of: i. Left atrioventricular valve endocardiosis (myxoid degeneration). (3 marks) ii. Subaortic stenosis. (3 marks) iii. Tetralogy of Fallot. (4 marks) Continued over page Veterinary Pathology Paper 2 Page 7 of 8
4. Answer five (5) of the following: (for a total of 30 marks) a) Briefly discuss the pathogenesis and major gross and histological features of beak and feather disease in Psittaciforme birds. (6 marks) b) Describe the biochemical changes and histological appearance of pyrrolizidine alkaloid toxicity in sheep. (6 marks) c) Provide two (2) examples of blood and/or urinary biomarkers for the assessment of renal function in small animals. Comment on how they may be used for diagnosis of renal disease and include their limitations. (6 marks) d) Explain the cause, typical signalment, gross appearance and histology of feline sarcoids. (6 marks) e) Describe the gross and histological lesions expected to be present in a case of bovine fungal abortion. (6 marks) f) Outline the methodology and use of fractional excretion for the assessment of renal function, electrolytes and mineral balance in horses. (6 marks) End of paper Veterinary Pathology Paper 2 Page 8 of 8