Pseudomonas aeruginosa

Transcription

1 تردد بكرتيا الزائفت الزجناريت ملرضى التهاب اجملاري البوليت يف مدينت العمارة Pseudomonas aerugiosa \4\1-2\1 Pseudomonas aerugiosa Pseudomonas aeruginosa 467

2 Amoxycillin, Gentamycin, Cefotaxaime, Carbenicillin, Aztreonam, Ciprofloxacin, Piperacillin, Cefazolin. Norfloxacin, Amikacin pseudomonas 94 0 aerugiosa Introduction (1) (2) (3) 468

3 Pseudomonas aerugiosa (4) (5) Nasocomial infection) (6,7) Haemolysin Lipase (8,9) Exotoxin phospholipase C Protease 469

4 in- vitro (Materials & Methods) (Equipment & Apparatus) - 1 p.aeruginosa (Centrifuge (Autoclave ) (Hot plate magnetic stirrer 37 (Spectrophotometer) ( Incubation) 470

5 45 (Water distilator) 200 (Electric oven) (Sensitive balance) (Millipore filter unit) (Light microscope) (ph meter) (Culture media) :

6 (11, 10) (Blood base agar) ( Brain heart infusion agar) (Agar-agar medium) (Urea base agar ) (Simon S citrate agar ) (MacConkey agar ) (Muller Hinton agar ) (Nutrient agar &broth ) Difico (U.S.A ) (11,10) 3 (Antibiotic disc) 4 25, Gentamycin 25 Amoxycillin Ciprofloxacin, Carbenicillin, 30 Cefotaxaime, 25 Aztreonam, 30 Cefazolin

7 Piperacillin, Amikacin (ATCC ) 30 Cefotaxime, 10 Norfloxacin, Standard Strain P. aeruginosa Different materials 6 (11,10) API 20 E Patient s samples \4\1-2\1) 10 (11,10) Isolation&Diagnosis Macroscopic appearance) 1 473

8 (11,10) (Microscopic appearance) :- (Gram (11,10) (Biochemical tests) Urease tests) (Oxidase tests) (Catalases tests) Indol production tests) Methyl red tests) (Voges proskauer tests) (Citrate utilization tests) 2 s stain) 3 (API 20 E Techneqe) :- 4 (Oil )

9 Antibiotic susceptibility 12 MacFarland tube 10 1) Disc diffusion tests Non (13,11) (Results&Discussion) lactose fermenter Single ( ) Motile Pyocyanin (15,16) Obligately polar flagellum 475

10 (16,4) P. aeruginosa 1 (API 20 E) 17,16, p. %11.4 Nosocomial infection %90-60 aeruginosa

11 %9.3 (%8.7) 20 (%8.6) P. aeruginosa 21 P. aeruginosa Cefazolin 2 Amoxycillin ( %90)30 (%100)

12 Cefotaxime Carbencillin 23 (%76) Cefotaxime Gentamycin (BroadSpectrum) (24 p. aeruginosa P. aeruginosa (%94) 31 Aztreonam Amikacin (%88) (%78) 9 2 P. aeruginosa Ciprofloxacin %76 (%90) (%66.4) Aztreonam (%72)23 (%46)15 (%92)30 Amikacin p. aeruginosa p. aeruginosa

13 (%28.7) (%19.9.) Antibiotic 28 Perfloxacin Ofloxacin Combination P. aeruginosa 1 479

14 480 2 P. aeruginosa Amoxycillin Cefazolin Carbencillin Cefotaxime Gentamycin Norfloxacin Piperacillin Ciprofloxacin Aztreonam Amikacin

15 Referances : 1-Bonadio,M;Meini, M. ; Spitaler, p.and Gigli, C current microbiological and clinical aspects of urinary tract infections. Eur. Urol. 40 : Marthez, J.L.and Baquero, F Interactions among strategies associated With bacterial infection : pathogenhcity, Epidericity and Antibiotic resistance. Clin. Micro. Reviews 15 (4): Ayliffe, g.a.fraise, A.P.;Geddes, A.M.and Mitchell, K Cotrol of hospital infection a practical Handbook 4 th ed.arnold London. Pp: Habbeb,F Isolationand identification of most common pathogens in vaginaof non pregnant woman in Baghdad. MSc. thesis. College of Medical and Health Technology. 5- Olayinka, a.t.;onile, B.A.and Olayinka,B.O.2004.Prevalence of multi-drug resistant p.aeruginosa isolates in surgical units of Ahmadu Bello University Teaching Hospital,Zaria, Nigeria.Annals of African Meadicine.3(1): Obritsch, M.D.Fish, D.N,; Maclaren, R.and June, R National surveillance of antimicrobial resistance in p.aeruginosa isolates obtained from Intensive Care Unit patients from Antimicrobial Agents and Chemotherapy.48(12): Miller, K.;John, A.;oneill, J.and Chopra, I.2004.E.coli mutators present an enhanced risk for emergence of antibiotic resistance during urinary try tract infections. Antimicrobial Agents and Chemotherapy. 48(1): Cotter,C.S.;Avidano, M.A.;Stringer, S.P.and Schultz, G.S.1996.Inhibition of proteases in pseudomona spp. 481

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17 resistant :Risk factors for acquisition and impact on outcomes. J.Antimicr Chem.22: Song,Z.;Wu,H.;Ciofu,O.;Hoiby,N.;Rygaard,J.; Kharazmi,A.and Mathee,K alginate is regractory to Th 1 immuno response and impedes host immune clearance in a mouse model of acut lung infection.j.med.micro.52: Pellegrino,F.P.C.;Teixeira,L.M;Carvalho,M.G.S.;Nouer,S.A ; Oliveire,M.P.;Sampaio,J.L.M.;Freitas,A.D.;Ferreira,A.L.P.; Amorim, E.L;Riley,L.W.and Moreira,B.M.2002.Occurrence of a multi-drug resistance p.aeruginosa clone in different hospital in Rio de Janeiro,Brazil.J.Clinc.Micro.40(7): Kang,C.I.;Kim,S.H.;Park,W.B;Lee,K.D.;Kim,H.B;Kim,E.C. ;Oh,M.D.and Choe,K.W.2005 Bloodstream in fections caused by antibiotic resistant gram negative bacilli:risk factors for mortality and impact of inappropriate initial antimicrobial therapy on outcome.antimicrobial Agents and Chemotherapy.49(20: Chanalm,C.;Bonnet,R.;Champs,C.D.;Sirot,D.;Labia,R.and Sirot,J.2000.Prevalence of Beta- Lactamase among 1072 clinical strains of proteus mirabilis:a2 years survey in a French Hospital.Antimicrobial Agents and Chemotherapy.44(7): Kriengkauykiat,J,;Porter,E.;Lomovskaya,O. and Wong- Beringer,A.2005.Use of an efflux pump inhibitor to 483

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19 Frequency of Pseudomonas aerogniosa isolates of urinary tract infection( UTI) patients in Al-Emarha city Ali.A.Saihod Technical Institute - Amara Abstract :- In this study One hundred urine samples obtained from patients suffering from urinary tract infection(uti), whom admitted to AL sadr generl Hospital for the period of 1/2 1/4/2009,in Emarha city. All urine samples were cultured on MacConkey agar and Blood Agar base, then Macroscopic, Miacroscopic appearance and Biochemical tests were done to diagnose the purity isolates of p.aeruginosa, which found with( 33%) while there were (28)isolates obtained. Results of susceptibility to c to antibiotics Amoxycillin, Gentamycin, Cefotaxaime, Carbenicillin, Aztreonam, Ciprofloxacin, Piperacillin, Cefazolin. Norfloxacin, Amikacin ) showed that this bacteria had resistant mode ranged between 6% to 100%, while it was also having wide range of sensitivity ranged between 0% to 94% for Antibiotics which listed. The study should be recommending to use antibiotic combination to reduce antibiotic resistance of bacteria and to treat patients with UTI 485