PREVALENCE OF FASCIOLA INFECTION AMONG SCHOOL CHILDREN IN SHARKIA GOVERNORATE, EGYPT ABSTRACT
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1 Journal of the Egyptian Society of Parasitology, Vol. 25, No.2, August 1995 J. Egypt. Soc. Parasitol., 25 (2), 1995: PREVALENCE OF FASCIOLA INFECTION AMONG SCHOOL CHILDREN IN SHARKIA GOVERNORATE, EGYPT By MOHSEN M. HASSAN, NAHED E. MOUSTAFA, LIALA A. MAHMOUD, BESSA E. ABBAZA AND MOHAMED H.A. HEGAB Department of Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt. ABSTRACT This study was performed on 1350 school children from 9 different villages in Sharkia Governorate to investigate the real situation of endemicity of fascioliasis in the area. Stool examination using modified Kato thick smear method was performed to detect Fasciola infection and other parasites. Those with negative stool samples were examined serologically by ELISA test to detect anti-fasciola IgG. All cases with positive anti-fasciola IgG were further examined by circum-oval precipitin test (COPT) against viable S.mansoni eggs to exclude the crossly reacted Schistosoma infections. Sixty nine cases were found to pass Fasciola eggs in their stool samples (5.1 %). Anti-Fasciola IgG was detected in the sera of 231 children (17.1 %) using ELISA test. Eighty four out of the 231 children were found positive by COPT and were considered as schistosomal cases. The remaining 147 who gave negative COPT were considered as Fasciola infections. All of the 69 Fasciola positive stool cases were found positive by ELISA test Hnd negative by COPT test. The sensitivity of stool analysis was 47% versus 100% sensitivity of ELISA, whereas the specificity of ELISA was 63%. The tota! number of Fasciola positive cases by ELISA and stool analysis were
2 serologically by ELISA test to detect anti-fasciola 19G> 11oreover, all cases with positive anti-fasciola IgG were also examined by circum-oval precipitin test (COPT) against viable S>mansoni eggs to exclude Schistosoma infections which may give false positive fascioliasis> Partially purified Fasciola antigen was used in an ELISA assay according to Hassan et at (1989) to detect anti-fasciola IgG> Fasciola g igantica adult worms, obtained from condemned bovine livers from slaughterhouse, were partially purified using Sephadex G-200 column according to the method of Mansour et at (1983» The gel filtration gave 4 peaks> The highest results were obained with peak II and IlL The two peaks were pooled and used as an antigen in the present study> Viable SJnansoni eggs, derived by centrifugation on different density gradients of percoll (Pharmacia) were used as an antigen (EI-Gamal and Moustafa, 1991) in a circum-oval precipitin test according to tile method described by Oliver Gonzalez (1954» RESULTS A total number of 1350 school childen from 9 different villages were parasitologically examined for fascioliasis> Sixty-nine cases were found to pass Fasciola eggs in their stool samples (5)1 %» Anti-Fasciola IgG was detected in the sera of 231 children (17 J %) using ELISA test against partially purified adult Fasciola antigen as shown in Table (n Out of 231 ELISA positive cases, 84 cases were found positive by COPT and were considered as schistosomai cases (Table 2» The remaining 147 who gave negative COPT were considered as Fasciola infections> All Fasciola positive cases by stool analysis (69) were found to be positive by ELISA test and negative by COPT tesl ELISA test could detect 78 Fasciola positive cases more than stool analysis> So, the sensitivity of stool analysis was 47% versus 100% sensitivity of ELISA, while the specificity of ELISA was 63% as shown in Table (3» The total number of Fasciola positive cases by ELISA and stool analysis were 147 cases among 1350 children> So, the prevalence rate was m9%>
3 cases among 1350 children indicating a prevalence of 10.9% among school children in Sharkia Governorate. This results highlighting the importm1ce of health education and snail control in decreasing the high prevalence. INTRODUCTION Human fascioliasis is a world wide problem, it is mainly caused by Fasciola hepatica and to a lesser extent by F.gigantica (Hardman etal., 1970). Human fascioliasis is becoming a public health problem among Egyptians (Osman, 1991). Many workers studied the prevalence of fascioliasis in Egypt. Ali et al. (1974) found that human fascioliasis became a health problem in Dakahlia Governorate. Moreover, Makled et al. (1988) reported that the prevalence of fascioliasis in Sharkia Governorte reached up to 8.7%. Also its prevalence in Alexandria (4.6%) was studied by Abou-Basha et al. (1990). Diagnosis of human fascioliasis by finding eggs in faeces lacks sensitivity since eggs may not appear during acute and chronic fascioliasis (De Wei! et al., 1984). In addition, false positive results are accounted in stool samples following ingestion of infected livers. So, the reference to serological tests are of value in many cases (Hillyer and Santigo, 1981). Keeping in mind that the cross reactions with other helminthic infections and false positivity with normal human sera, appeared with serological tests, constitute a major problem for specific detection of fascioliasis (Hillyer and Capron, 1976). One has to consider and try to eliminate reactivity specially with sera from patients with schistosomiasis (Hassan et al and Khalil et al., 1990) to consider the obtained results. SUBJECTS AND METHODS This study was performed on 1350 school children (1101 males and 249 females), aged 6-12 years (mean ±SD: 1O.18±2.43) from 9 different villages in Sharkia Governorate. For every child, stool examination using modified Kato thick smear method (Martin and Beaver, 1968) was performed to detect Fasciola infection and other parasites. Ten ml. urine sample was examined using nuclepore filtration technique (Peters et al., 1976) for S.haematobium egg counting. Those with negative stool samples were examined
4 Table (1): Stool examination and ELISA for detection of Fasciola infection. Test. Total number No. of +ve cases 0/0 positivity Stool ELISA Table (2): COPT among cases with ELISA positive anti--fasciola IgG. Test Total number No % COPT +'ve COPT -ve Table (3): Sensitivity and specificity of the ELISA test. Positive by test False +ve Sensitivity Specificity Stool % 1000/0 examination ELISA % 630/0 DISCUSSION In Egypt, human fascioliasis have been reported by Ali et al. (1974) and EI-Shazly et aj. (1991) in Dakahlia Governorate, Farag et at (1979)~ Abou-Basha et a1. (1990) and Farag et al. (1995) in Alexandria Governorate, Ismail et al (1988) and Makled et al. (1988) in Sharkia Governorate and Abdel-Rahman et 31. (1995) in Menoufia Governorate. In the present work, 69 out of 1350 children (5.10/0) had Fasciola eggs by stool examination with a sensitivity of 47%. De \Veil et al. (1984) attributed the low sensitivity of stool examination of difficulty in finding Fasciola eggs during acute and chronic fascioliasis. Moreover, false fascioliasis due to ingestion of infected liver \-vas reported by Cheesbrough (1987) as another cause oflow sensitivity+ So, the sole dependence on stool examination will miss many cases as mentioned by Makled et al. (1988). The reference to serological tests is of 546..
5 accurate value in many ca~es (Hillyer and Santigo, 1981). Using ELISA test in the present work, 231 cases out of 1350 school children were found positive. COPT was carried out to exclude those with schistosomiasis from the recorded ELISA positive cases as cross reactions between schistosomiasis and fascioliasis were reported by many workers (Khalil et at, 1990 and Osman & Helmy, 1994). COPT revealed that 36.4% ofelisa positive cases had anti-schistosoma antibodies in their sera, which is in agreement with the results previously obtained by Hassan et al. (1989) who reported t':lat 25% of sera ofschistosomiasis gave false positive reactions with Fasciola antigen. In the present work, 147 Fasciola cases proved by positive ELISA were negative by COPT. Moreover, all positive stool cases had detectable anti Fasciola antibodies by ELISA revealing that the sensitivity of ELISA was 100%. These results are inagreement witl! those of Shaker et al. (1990) and Habeeb et al. (1992) who recorded sensitivities around 66%. As a partially purified Fasciola antigen was used, the specificity of ELISA was 63%, which is lower than that obtined by Shaker et al. (1994) who used a highly purified Fasciola antigen. Mourad (1995) reported that fascioliasis became endemic in Sharkia Governorate. The prevalence of human. fascioliasis among rural school children was 10.9% in the present work. This was slightly higher than that recorded by Makled et al. (1988) who reported a prevalence of8.7% in other localities in Sharkia. The increasing prevalence might be due to inefficient snail control in the governorate. Farag et al. (1995) highlighted the importance of wing snail control as they reported a decrease in prevalence of fascioliasis in Alexandria Governorate from 5.2% in 1990 to less than 2% in 1994 after the B.alexandrina snail control and without administration of any fasciolicidal drug. REFERENCES Abdel Rahman, M.; Esmat, G.E.; Ramzy, I.I.; El.Kady, N. and Abdel Wahab, M.F. (1995): Prevalence of human fascioliasis in 28 villages in Menoufia Governorate, Egypt. Represented to 2nd Ann. Congr. ESTIP, Cairo, 3-5 January. Abou Basha, L.M.; Salem, A.Z. and Fadali, G,H. (1990): Human fascioliasis. Ultrastructure study of liver before and after
6 bithional treatment. J. Egypt Soc. Parasitol., 20(2): Ali, M.E.; EI... Kannishy, M.H.; EJ.Kbo]J, E.S~; Hegazi, M..M.; Khashaba, M.R.; Rifaay, M.R.; EI Bassousy, E.M.M.; Sheir, Z.M. and Rifaat, M.A. (1974): Clinical and laboratory studies on human fascioliasis in Egypt. Mansoura Med. Bull., 3&4: Cheesbrough, M. (1987): Medical Laboratory Manual for Tropical Countries. 2nd 00. buttef\\/orth-neinemann Ltd. Oxford. De Weil, N.S.; Hillyer, G.V. and Phacheco, E. (1984): Isolation of F.h.epatica genus specific antigens. lnt. J. Parasito1., 14(2): EI-Gamal, R.L.R. and Moustafa, N.A. (1991): Comparative study between ELISA and COPT using Schistosolna 'lnansoni and S. haelnatobium eggs in diagnosis of schistosomiasis mansoni. E.M#J.; 9: EJ-ShazJy, A~M..; Handousa, A~E.; Yossef, M.E.; Rizk, H. and Hamouda, M. (1991): Human fascioliasis: A parasitic health problem in Dakahlia. J. Egypt. Soc. Parasitol., 21(2): Farag, H.F.; Abou Basha, L.M. and Salem, A.I. (1995): A longitudinal study on the transmission of human fascioliasis. Represented to 2nd Ann. Congr. ESTIP, Cairo, 3-5 January. Farag, R.G.; Barakat, R.M.R.; Ragab, M. and Omar, E. (1979): A focus of human fascioliasis in, the Nile Delta, Egypt. J. Trap. Med. Hyg., 82: Habeeb, Y.S.lVl.; Seleem, M~A.. ; Ali, M.S. and Attia, M.M. (1992): Assessment of humoral and cellular immunity in human fascioliasis. New Egypt. J. Med., 6: Hardman, E.W.; Jones, R.L.H. and Davies, A.H. (1970): Fascioliasis a large outbreak. Br. Med. J., 3: 502. Hassan, M.M#; Fargha]y, AeM.; El..Gamal, R.L.R. and EI Ridi, A.M.S. (1989): Cross reactions in immunodiagnosis of patients infected with Schistosoma, Fasciola' and Heterophyes using ELISA. J. Egypt. Soc. Parasitol., 19(3): Hillyer, G'IV. and Capron, A. (1976): Immunodiagnosis of human fascioliasis by counter-electrophoresis. J. Parasito!., 62: Hill}1 r,g.v. and De Weil, N.S. (1981): Serodiagnosis of
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