Khoa Thú y, H c vi n Nông nghi p Vi t nam. Ngày g i bài: Ngày ch p nh n:

Transcription

1 Vietnam J. Agri. Sci. 2017, Vol. 15, No. 7: T p chí Khoa h c Nông nghi p Vi t Nam 2017, 15(7): * Khoa Thú y, H c vi n Nông nghi p Vi t nam lai.tl.huong@gmail.com Ngày g i bài: Ngày ch p nh n: K t qu kh o sát tình hình m c b nh viêm bú bò s u t n t l m c b nh và th nghi u tr i Xí nghi uôi bò s ng, Gia Lâm - Hà N i. K t qu ki m tra b y t l viêm vú lâm sàng trung bình th v i viêm vú th n (10,43% và 25,36% California Mastitis Test - i gian làm m t màu c a Xanh Methylen cho th y 90% m u s a có th d ng trong vòng ti ng. m u s a làm m t màu thu c th trong vòng 30 phút là 3,33%. T n khai thác khác nhau thì kh c b nh viêm vú là khác nhau. m c b nh cao nh t u l y s m d n tháng khai thác th 4; t tháng th 6 tr n c n s a, t l bò m c b nh viêm vú l a tu nh n t l m c b nh, bò càng già thì kh c b ng bò có t l máu ngo i cao thì t l m u tr th nghi m b ng Neomycin/Amoxicillin k t h p v a bên trong b u vú, h lý p lý cho hi u qu u tr i cao. T khoá: Viêm vú bò s a, y u t u tr. Prevalence of Bovine Mastits, Affecting Factors and Experimental Treatment in Phu Dong Dairy Farm ABSTRACT The aims of present study were to evaluate the prevalence of mastitis, identify factors influencing disease incidence and test treatment methods in Phu Dong dairy farm in Gia Lam - Ha Noi. The results by California Mastitis Test showed that the prevalence of clinical mastitis was lower than that of sub-clinical cases (10.43% and 25.36%). Based on methylene blue decolorization test, 90% of testing milk samples could be used within hours. The percentage of milk samples discolorized by the dye within 30 minutes was 3.33%. The risk of infection was influenced by the stage of milking. The highest incidence was found in the first month of lactation then reached lowest point at fourth month. The infection rate continued to increase from sixth month toward the end of the lactation period. Age was positively correlated the disease incidence. Cows with higher levels of cross-bred rate had higher rates of mastitis. Treatment with neomycin/amoxicillin combined with pumping inside the infected udders and proper care was effective. Keywords: Bovine mastitis, affecting factors, CMT, treatment, neomycin, amoxicillin. 905

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3 M ph n ng Tr ng thái và màu c a h n h p s a - thu c th - - Tr ng thái c a h n h i Âm tính - Gi nguyên màu thu c ± - S t nh Nghi ng - Màu c a h n h i + - S - Thu c th n màu S ng t bào thân (TB/1 ml s a) < S a quánh l n trôi Thu c th S > Th i gian m t màu ng s vi khu n/ml (x10 6 ) Ph m ch t s a X p lo i Bi n pháp x lý < 20 phút 20 R t x u 4 t i ch 20 phút - 2 gi 4-20 X u 3 i ch và dùng cho gia súc 2 gi - 5,5 gi 0,5-4 Trung bình 2 Cho bán t do không quá 12 gi > 5,5 gi < 0,5 T t 1 Cho bán t do không quá 24 gi 907

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5 t ki m tra S m u ki m tra S m u t ,00 t ,71 t ,00 t ,50 T ng ,43 t ki m tra S m u/s bò S m u Âm tính S m u Nghi ng +, t 1 74/ , , ,68 t 2 80/ , , ,00 t 3 76/ , , ,31 t 4 72/ , , ,62 T ng 302/ , , ,16 T ng (+) t S m u < 30 phút 2 gi n 2 gi 1 phút n 3 gi Th i gian m t màu 3 gi 1 phút n 4 gi 4 gi 1 phút n 5 gi t t t t T ng ,33 6,67 13,33 15,00 38,33 23,34 > 5 gi 909

6 X ò ö ä ä n khai thác theo dõi < 1 tháng 1-6 tháng > 6 tháng C n s a m c theo dõi m c theo dõi m c theo dõi m c Tháng th nh t , , , ,95 Tháng th hai , , , ,63 Tháng th ba , , , ,00 T ng , , , ,51 HF2 HF3 Gi ng bò S bò theo dõi S bò b viêm vú S bò theo dõi S bò b viêm vú Tháng th nh t , ,99 Tháng th hai , ,75 Tháng th ba , ,19 T ng , ,55 910

7 ,86 16,67 13,79 11,63 12,73 16, ,

8 X ò u tr S u tr (con) S ca kh i b nh (con) kh i Ngày kh i I ,2 II ,5 Belay Beyene và Tadele Tolosa (2017). Epidemiology and financial impact of bovine mastitis in an Animal production and research center and small holder dairy farms in Horoguduru Wollega Zone, Western Ethiopia. Journal of Dairy, Veterinary & Animal Research, 5(4). Claxton P and D. Ryan (1993). Bovine mastitis. In Australian Standard Diagnostic Techniques for Animal Diseases. L. Corner and T. Bagust, ed. CSIRO for the Standing Committee on Agriculture and Resource Management, East Melbourne, Victoria. Detillieux J.C, Kehsli M.E., Freeman A.E., Fox L.K., and Kelley D.H. (1995). Mastitis of periparturient Hoslstein cattle: a phenotypic and genetic studies. Joural of Daisy science, pp. 78 Factsheet. Haas Y de; R.F. Veekamp; H.W. Barkema; Y.T. Grohn & Y.H. Schukken (2004). Associations between pathogen- specific cases of clinical mastitis and somatic cell count pattesns Department of Health Management, Atlantic Veterinary college, Canada, pp Hamann J. (1991). Milking related teat tissue changes as a predisposing factor for mastitis. Institute for 912

9 Hygiene, Dairy Research Centre, 2300 Kiel, Hermann Weigmann- Strabe 1, Germany. Hogan J. S., Gonzalez R. N., Harmon R. J., Nickerson S. C., Oliver S. P., Pankey J. W. and Smith K. L. (1999). Laboratory handbook on bovine mastitis. National mastitis council, Inc., Madison, Wisconsin, USA. Martin F., Failingk., Wolter W., Kloppert B., and Zschock M. (2002). Effect of parity and period of Lactation on prevalence of mastitis pathogens is quarters with high somatic cell count (SCC > /ml). Milchwissens Chaft McDougall S. (2003). Intramammary treatment of clinical mastitis of dairy cows with a combination of lincomycin and neomycin, or penicillin and dihydrostreptomycin. N Z Vet J., 51(3): m vú Rajeev Ranjan M. K. Gupta and K. K. Singh (2011). Study of bovine mastitis in different climatic conditions in Jharkhand, India. Veterinary World, 4(5): Subir Kumar Nandy and K. V. Venkatesh Application of Methylene blue dye reduction test (MBRT) to determine growth and death rates of microorganisms. African Journal of Microbiology Research, 4(1): Tahmina Bilkis, Md. Manirul Islam, M.C. Sumy, Md. Nasim Ali Mandal and Gazi Md. Noor Uddin (2013). Rapid Estimation of Quality of Raw Milk for its Suitability for Further Processing in the Dairy Industries of Bangladesh. International Journal of Dairy Science, 8: XIV(5): ,., Nhà Pyörälä S. (2008). Mastitis in post-partum dairy cows. Reprod Domest Anim., 43: [PubMed] Rahmeto Abebe, Hagere Hatiya, Mesele Abera, Bekele Megersa and Kassahun Asmare (2016). Bovine mastitis: prevalence, risk factors and isolation of Staphylococcus aureus in dairy herds at Hawassa milk shed, South Ethiopia. BMC Vet Res., 12: 270. doi: /s Wilson, J.D., Gonzalez, R.N., Das, H.H. (1997). Bovine mastitis pathogen in New York and Pennsylvania: prevalence and effects on somatic cell count and milk production. Journal of Dairy Science. Wilson J.D., Gonzales R.N., Case K.L., Garrison L.L. and Grohn Y.T. (1999). Comparison of seven antibiotic treatments with no treatment for bacteriological efficacy against bovine mastitis pathogens. J Dairy Sci., 82(8):