European Antimicrobial Resistance Surveillance System (EARSS) in Scotland: 2004
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1 European Antimicrobial Resistance Surveillance System (EARSS) in Scotland: 2004 SECOND ANNUAL REPORT MJ Coyne 1, SJ Dancer 1, G Edwards 2, 3, D Morrison 2. 1 Health Protection Scotland, 2 Scottish MRSA Reference Laboratory, 3 Scottish Meningococcus and Pneumococcus Reference Laboratory. SUMMARY This is the second annual report describing a national surveillance scheme for Scotland. This scheme collects data on invasive Staphylococcus aureus and Streptococcus pneumoniae from all NHS clinical microbiology laboratories. During 2004, 1691 S.aureus isolates were received. Six hundred and ninety (41%) of these were resistant to methicillin; 501 (73%) were of type EMRSA-15, 163 (24%) were EMRSA-16 and 26 (4%) were of other type. Five hundred and thirty eight S.pneumoniae isolates were received during Eighteen (3.4%) were non-susceptible to penicillin and 69 (13.0%) were non-susceptible to erythromycin. These data provide a baseline for surveillance and a means for the identification of significant trends for the future. INTRODUCTION The European Antimicrobial Resistance Surveillance System (EARSS) is an international network of national surveillance systems which was started in 1998 with funding from the European Commission. 1,2 The scheme aims to collect comparable and reliable antimicrobial resistance data on invasive isolates of Staphylococcus aureus (including methicillin-resistant Staphylococcus aureus (MRSA)), Streptococcus pneumoniae, Escherichia coli and Enterococcus faecium/faecalis. At present, information is routinely collected from more than 750 microbiology laboratories in 28 countries. The programme provides an opportunity for anonymised patient specific data on bacteraemias to become available from all NHS hospitals in Scotland. EARSS has been in operation in Scotland since January 2002 for S.pneumoniae and July 2002 for S.aureus. At present, no data is collected for E.coli and E.faecium/faecalis. METHODS Every diagnostic microbiology laboratory in Scotland sends all first blood or cerebrospinal fluid (CSF) isolates of S.aureus and S.pneumoniae from every patient to the Scottish MRSA and Scottish Meningococcus and Pneumococcus Reference Laboratories respectively. These laboratories confirm the identification and determine a range of antimicrobial susceptibilities for each isolate. The Reference Laboratories forward these data to HPS, who then send them to the UK coordinating centre at HPA Colindale, who forward it, along with data from England, Wales and 1
2 Northern Ireland, to the European base at the National Institute of Public Health and the Environment (RIVM), in Bilthoven, Holland. Investigations for S.aureus All isolates were tested for the meca gene by Polymerase Chain Reaction (PCR), and for susceptibility to eighteen (MRSA) or six (MSSA) antibiotics including methicillin by Stokes disc diffusion method, or from April 2004, by an automated method. BHI agar containing 5 mg/l of vancomycin was used to screen for reduced susceptibility to glycopeptides. Where meca PCR was inconsistent with methicillin susceptibility by Stokes or automated method, isolates were further tested by determining oxacillin and cefoxitin MIC s (E-test method, Bio-Stat, Manchester, UK) as follows: suspensions of test organism were prepared to 0.5 McFarland standard and used to inoculate Mueller Hinton agar (Oxoid) containing 2% sodium chloride. E-test strips were applied to the agar and plates were incubated at 35 C for 24 hours. Mupirocin high-level resistance was confirmed by PCR amplification of mupa gene. The hospital department from which the specimen originated was recorded, as well as possible association with an intravascular device. Investigations for Streptococcus pneumoniae Isolate identities were confirmed as pneumococci by antigen testing. Co-agglutination testing was used for serogrouping/typing. The E-test method (Bio-Stat, Manchester, UK) was used for antimicrobial susceptibility measurement 3 : suspensions of test organism were prepared to 0.5 McFarland standard and used to inoculate Mueller Hinton agar plates containing 5% lysed horse blood (Oxoid). E-test strips were applied to the agar and plates were incubated at 37 C overnight in a 5% carbon dioxide atmosphere. Susceptibilities to penicillin, erythromycin and ciprofloxacin were determined for each isolate. RESULTS S.aureus Table 1 shows the number of invasive staphylococcal isolates received at the Scottish MRSA Reference Laboratory during the inclusive period January to December MRSA makes up 41% of all S.aureus bacteraemias. MRSA molecular typing The results from MRSA molecular typing are shown in Figure 1 and Table 2. EMRSA-15 is the predominant type in Scotland, accounting for 72.6% of all isolates reported. The percentage of EMRSA-16 is 23.6%. The other group accounts for 3.8% of all isolates and is composed of nine different types. Antimicrobial susceptibilities for S.aureus Figure 2 and Table 3 detail a selection of MRSA antimicrobial susceptibilities. Six point five percent of Scottish blood isolates were resistant to fucidin, 11.1% to gentamicin, 4.3% to mupirocin, 3.2% to rifampicin, 4.6% to tetracycline and 16.2% to 2
3 trimethoprim. Most isolates (78.4%) were resistant to erythromycin, and 20% of the remaining 22% susceptible to erythromycin were of type EMRSA-15. MSSA susceptibilities to erythromycin and fucidin are listed in Table 4. The fucidin resistance rate among MSSA is comparable to the MRSA rate: 8.8% MSSA nonsusceptible, compared with 6.4% for MRSA. However, there are significant differences between the erythromycin rates: 7.6% MSSA were resistant to erythromycin, compared with 78.4% for MRSA. Figure 3 and Table 5 detail the proportion of S.aureus / MRSA bacteraemias by hospital speciality. The highest MRSA rates were found in the following departments: HDU/ICU (51.6%), paediatric ICU (50.0%) and Care of the Elderly (49.4%). The lowest MRSA rates (and total S.aureus bacteraemias) were found in paediatrics and obstetrics/gynaecology with rates at 0 and 11.1% respectively. Figure 4 and Table 6 detail the MRSA type by hospital speciality. Table 7 details the association between intravascular device and infection by Division. Definitive details on intravascular device association were provided for 379 isolates. Two hundred and thirty-six (67%) of these were not associated with intravascular device and 143 (38%) were associated with intravascular device. S.pneumoniae Figure 5 and Table 8 show the number of invasive isolates received at the Scottish Pneumococcal and Meningococcal Reference Laboratory during The majority of specimens (97%) come from blood, though this may not truly reflect the proportion since some patients may have an isolate from both blood and cerebrospinal fluid but first isolates only are reported. Susceptibilities to penicillin, erythromycin and ciprofloxacin from all laboratories are shown in Figure 6 and Table 9. Table 10 shows susceptibility data by Division. Overall the rate for penicillin non-susceptible S.pneumoniae was 3.4%, erythromycin non-susceptible S.pneumoniae 12.3% and ciprofloxacin non-susceptible S.pneumoniae 23.7%. Figure 7 and Table 11 show the serotype distribution for all isolates. DISCUSSION The proportion of methicillin-resistant staphylococci in total numbers of staphylococci from blood has remained consistent between 2003 and 2004: 41% for both years. Scotland s rate is not significantly different to that of the rest of the UK, but still remains amongst the highest in Europe. 2 There may be an association between S.aureus bacteraemia and the presence of an intravascular device and it is important to improve the quality of reporting for this field. The rate of penicillin and erythromycin resistance in invasive pneumococci is relatively low in Scotland, at 3.4% and 13.0% respectively. There has been a small increase since 2003 (from 3.1% 3
4 penicillin non-susceptible and 12.3 erythromycin non-susceptible) however only time will determine if this remains to be the case. ACKNOWLEDGEMENTS We would like to acknowledge all clinical and scientific microbiologists who have collaborated in this programme and all staff at the Scottish MRSA and Scottish Meningococcus and Pneumococcus Reference Laboratories. Without their help, we could not have introduced this surveillance scheme, nor obtained this data. REFERENCES 1. Bronzwaer SL, Goettsch W, Olsson-Liljequist B, Wale MC, Vatopou AC, Sprenger MJ. European Antimicrobial Resistance Surveillance System (EARSS): objectives and organisation. Euro Surveill 1999; 4: Kyaw MH, Christie P, Clarke SC, Jones IG, Campbell H. Invasive pneumococcal disease in Scotland : use of record linkage to explore associations between patients and disease in relation to future vaccination policy. Clin Infect Dis 2003; 37:
5 Table 1. Episodes of S.aureus bacteraemia in Scotland reported through EARSS Period No. S.aureus No. MSSA No. MRSA %MRSA Jan-Mar Apr-Jun Jul-Sep Oct-Dec Total MSSA: methicillin-susceptible S.aureus; MRSA: methicillin-resistant S.aureus 5
6 Fife North Glasgow Lanarkshire Tayside Lothian Universities Grampian Argyll & Clyde South Glasgow West Lothian Highland Dumfries & Galloway Figure 1. MRSA isolates by Division, Other E16 E15 No. isolates Orkney Shetlend Western Isles Yorkhill Golden Jubilee Forth Valley Ayrshire & Arran Borders
7 Table 2. MRSA isolates by Division, Division Name No. MRSA E15 E16 Other Argyll & Clyde Ayrshire & Arran Borders Dumfries & Galloway Fife Forth Valley Golden Jubilee Grampian Highland Lanarkshire Lothian Universities North Glasgow Orkney Shetland South Glasgow Tayside West Lothian Western Isles Yorkhill Total Percentage
8 Rifampicin Mupirocin Tetracycline Figure 2. EARSS MRSA susceptibility data, Non susceptible Susceptible No. isolates Erythromycin Trimethoprim Gentamicin Fucidin
9 Table 3. EARSS MRSA susceptibility data (690 isolates tested), January- December Antimicrobial No. Resistant No. Intermediate No. Sensitive % Non susceptible Erythromycin Fucidin Gentamicin Mupirocin 30* Rifampicin Tetracycline Trimethoprim *Mupirocin resistant isolates: 9 low level, 21 high level. Table 4. EARSS MSSA susceptibility data (1000 isolates tested), Antimicrobial No. Resistant No. Intermediate No. Sensitive % Non susceptible Erythromycin Fucidin MIC breakpoints (mg/l): erythromycin intermediate >0.25<1, erythromycin resistant 1; fucidin intermediate 1-2, fucidin resistant 2; gentamicin intermediate >1 and <2, gentamicin resistant 2; mupirocin low level 8 and 256, mupirocin high level 512; rifampicin intermediate >0.06<0.12, rifampicin resistant 0.1; tetracycline intermediate >1<2, tetracycline resistant 2; trimethoprim intermediate >0.5<1, trimethoprim resistant 1. 9
10 Figure 3. Episodes of S.aureus bacteraemia in Scotland reported through EARSS 2004, by speciality MRSA MSSA No. isolates Obs/Gyn Paediatric ICU ID Paediatrics Oncology Orthopaedic Care of Elderly Other Unknown A&E HDU/ICU Surgery Renal Not stated Medicine 10
11 Table 5. Episodes of S.aureus bacteraemia in Scotland reported through EARSS 2004, by speciality. Department S.aureus MRSA % MRSA A&E Care of Elderly HDU/ICU ID Medicine Obs/Gynae Oncology Orthopaedic Other Paediatric ICU Paediatrics Renal Surgery Unknown Not stated Total
12 Medicine Not stated Renal Surgery HDU/ICU Unknown Care of Elderly Other A&E Oncology Figure 4. MRSA type by speciality Other E16 E15 No. isolates Paediatrics Obs/Gynae Paediatric ICU Infectious diseases Orthopaedic
13 Table 6. MRSA type by speciality. HOSPITAL DEPARTMENT E15 E16 Other Paediatrics Obs/Gynae Paediatric ICU Infectious diseases Orthopaedic Oncology A&E Other Unknown Care of Elderly HDU/ICU Surgery Renal Not stated Medicine
14 Table 7. Intravascular device association by Division. Division No Yes Unknown Not stated Total Argyll & Clyde Ayrshire & Arran Borders Dumfries & Galloway Fife Forth Valley Golden Jubilee Grampian Highland Lanarkshire Lothian Universities North Glasgow South Glasgow Tayside West Lothian Yorkhill Total
15 Figure 5. Invasive EARSS S.pneumoniae isolates, No. isolates Jan-Mar Apr-Jun Jul-Sep Oct-Dec CSF Blood Culture CSF = cerebrospinal fluid Table 8. Invasive EARSS S.pneumoniae isolates, Period No. S.pneumoniae No. Blood Culture No. CSF Jan-Mar Apr-Jun Jul-Sep Oct-Dec Total
16 Figure 6. EARSS susceptibility data S.pneumoniae, January to December No. isolates Non susceptible Susceptible Penicllin Erythromycin Ciprofloxacin Table 9. EARSS susceptibility data S.pneumoniae (532 isolates tested)*, January to December Antimicrobial No. In Group % Resistance Penicillin low level Penicillin high level 0 0 Erythromycin intermediate Erythromycin resistant Ciprofloxacin intermediate Ciprofloxacin resistant * not all isolates were tested against all antibiotics MIC breakpoints (mg/l): ciprofloxacin intermediate >1 and <4; ciprofloxacin resistant 4; erythromycin intermediate >0.25 and <1; erythromycin resistant 1; penicillin low level >0.064 and <2; penicillin high level 2. 16
17 Table 10. Invasive S.pneumoniae isolates received through EARSS programme, January-December 20043, by trust (532 isolates tested)*. Division Name No tested PNSP ENSP Western Isles 2 0 (0.0%) 0 (0.0%) Dumfries & Galloway 13 1 (7.7%) 2 (15.4%) Highland 16 0 (0.0%) 3 (18.8%) Borders 18 1 (5.6%) 2 (11.1%) West Lothian 19 0 (0.0%) 1 (5.3%) Forth Valley 26 2 (7.7%) 5 (19.2%) Ayrshire & Arran 27 0 (0.0%) 2 (7.4%) Yorkhill 27 2 (7.4%) 7 (25.9%) Fife 29 0 (0.0%) 6 (20.7%) Argyll & Clyde 34 1 (2.9%) 6 (17.6%) Grampian 46 0 (0.0%) 4 (8.7%) South Glasgow 47 3 (6.4%) 2 (4.3%) Tayside 48 1 (2.1%) 8 (16.7%) North Glasgow 56 1 (1.8%) 1 (1.8%) Lanarkshire 57 2 (3.5%) 11 (19.3%) Lothian Universities 67 4 (6.0%) 9 (13.4%) Total (3.4%) 69 (13.0%) * not all isolates were tested against all antibiotics PNSP = penicillin non-susceptible S.pneumoniae; ENSP = erythromycin nonsusceptible S.pneumoniae; MIC breakpoints (mg/l): PNSP >0.064; ENSP >
18 PNE035 Other PNE023 PNE033 PNE022 PNE020 PNE019 PNE018 PNE017 PNE016 PNE013 PNE014 PNE015 PNE012 PNE011 Figure 7. S.pneumoniae serotype distribution Serotype 18 No. isolates PNE001 PNE003 PNE004 PNE005 PNE006 PNE007 PNE008 PNE009 PNE010
19 Table 11. S.pneumoniae serotype distribution. Serotype No. isolates PNE PNE PNE PNE005 1 PNE PNE PNE PNE PNE010 5 PNE011 8 PNE PNE013 2 PNE PNE015 7 PNE016 2 PNE017 1 PNE PNE PNE PNE PNE PNE033 3 PNE035 4 Other 11 19
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