Functional investigation of a QTL region affecting resistance to Haemonchus contortus in sheep
|
|
- Bennett Booth
- 6 years ago
- Views:
Transcription
1 Functional investigation of a QTL region affecting resistance to Haemonchus contortus in sheep Guillaume Sallé 2, Carole Moreno 1, Julien Ruesche 1, Frédéric Bouvier 1, Mathias Aletru 1, Jean-Louis Weisbecker 1, Françoise Prévot 2, Jean-Paul Bergeaud 2, Cathy Trumel 3, Christelle Grisez 2, Dominique François 1, Andres Legarra 2, Emmanuel Liénard 2,3, Philippe Jacquiet 2,3 1 INRA Animal Genetics Department (UR631, UE632, UE) 2 INRA Animal Health Department (UMR 1225) 3 Toulouse University, INP - Ecole Nationale Vétérinaire de Toulouse EAAP 2013, Nantes, France
2 INTRODUCTION Gastro-intestinal nematodes : a curse for sheep breeding Impact sheep health and welfare Strong economical burden for breeders (~85 M in the UK Nieuwhof & Bishop 2005) Anthelminthic resistant populations have been selected for worldwide Breeding for more resistant animals could help controlling nematodes in a more sustainable fashion ~30 % of the observed variation in resistance due to the genetic background Strong between breeds differences Need to know the genes : good understanding of mechanisms + improve breeding schemes.02
3 Our working basis (Aumont et al. 2003, Terefe et al., 2007) Martinik Black-Belly Subtropical breed Resistant Romane French meat breed Susceptible Martinik sheep are far more resistant than the Romane sheep to H. contortus 3 to 10 fold less excreted H. contortus eggs Phenotypic differences also observed on the immunological background Higher and quicker eosinophilic infiltration of the abomasal mucosa IL5 and IL13 over-expressed in Martinik abomasal mucosa Key differences observed at 1 st infection Next question: what are the genetic mechanisms controlling these differences?.03
4 SUMMARY OAR12: an interesting candidate Investigating the functional properties of this region Conclusion & perspectives.04
5 Looking for regions associated to H. contortus resistance Martinik Romane F Back-cross B F C B F C Identify regions of the genome affecting resistance to H. contortus 1,000 lambs experimentally challenged twice Measured for Fecal Egg Counts and Haematocrit Genotyped for 50K SNP Choose one interesting region.05
6 Association of 4 SNPs with resistance to Haemonchus contortus One region affecting FEC each time the sheep faced H. contortus Second highest effect (0.19 σ p ) Found in other sheep populations: Sarda*Lacaune (Moreno et al., 2006) Merino (Beh et al., 2002) Soay sheep (Beraldi et al., 2007) G. Sallé et al., J. Anim. Science,
7 The 4-SNP allelic effects Effect (phen. std) 0,2 0,1 0-0,1-0,2-0,3-0,4-0,5-0,6-0,7-0,8-0,9-1 -1,1-1,2-1,3 Allelic effect (phenotypic standard deviation) Other alleles: 4SNP_Un Alleles with favorable effect 4SNP_R Two extreme alleles with unfavorable effects 13 alleles have been identified 3 favorable alleles versus 2 very unfavorable alleles One allele from the Romane breed within the favorable alleles (!) Martinik (resistant) origin Romane (susceptible) origin.07
8 SUMMARY OAR12: an interesting candidate Investigating the functional properties of this region Conclusion & perspectives.08
9 The experimental design Martinik Romane F Back-cross B F C B F C Identify regions of the genome affecting resistance to H. contortus Choose one interesting region BC x BC matings to generate offsprings Selection of allelic carriers Functional comparison.09
10 4SNP_R Functional validation BC x BC sheep 4SNP_Un Genotyping Two groups of BCxBC sheep selected on the 4SNP alleles they inherited : 4SNP_R animals carried the favorable alleles 4SNP_Un animals inherited neutral alleles - Determination of the inherited alleles - Estimation of the genomic background effect Effect (phen. std) 0,2 0,1 0-0,1-0,2-0,3-0,4-0,5-0,6-0,7-0,8-0,9-1 -1,1-1,2-1,3 Allelic effect (phenotypic standard deviation) Not found in the BCxBC flock Alleles with high favorable effect : 4SNP_R Other alleles: 4SNP_Un.010
11 Functional validation 2 BC*BC groups based on their 4SNP alleles Equivalent genomic value between the two groups Comparison of egg excretions Comparison of worm burden, females fertility and gene expressions 4SNP_R 4SNP_Un 44 challenged (16 vs 28) D0 15 dpi 18 dpi 21 dpi 24 dpi 27 dpi 30 dpi FEC FEC FEC FEC FEC Hematology Hematology Necropsy.011
12 An effect on fecal egg counts FEC in 4SNP-based groups * FEC (eggs/g of feces) * * 0 FEC18 FEC21 FEC24 FEC27 FEC30 4SNP_R 4SNP_Un 4SNP_R sheep excreted less eggs than 4SNP_Un.012
13 The effect on FEC could be explained by a difference in female worms fecundity For each BC*BC infected sheep: The length of 35 H. contortus females was measured The number of in utero eggs of 20 H. contortus females counted Avg. female worms length( mm) Female length * 4SNP_R 4SNP_Un No. eggs in utero Avg. fertility of female worms * 4SNP_R 4SNP_Un.013
14 No differences for worm burden 4SNP_R lambs also exhibited significantly less blood loss at 30 dpi.014
15 Any candidate genes? Selection of the most frequent alleles in each group: GAAG MBB vs GACA RMN (N = 9) (N =8) Effect (phen. std) Allelic effect (phenotypic standard deviation) 0,2 0,1 0-0,1 0-0, ,3-0,4-0,5-0,6-0,7-0,8-0,9-1 -1,1-1,2-1,3 Gene expression compared between carriers - Set of differentially expressed genes from micro-array experiment between pure breeds MBB and RMN sheep (Liénard et al., 2011) - PAPP-A2 (pappalysin) gene underlying the 4SNP region (regulation of Insulin-like Growth Factor (IGF) activity).015
16 Any candidate genes? IL-13 IL-4 No. IL3 mrna copies /100 REF mrna copies * No. IL4 mrna copies /10 4 REF mrna copies * Stronger Th2 response Abomasal mucosa, 30 days post-infection PAPP-A2 PAPP-A2 was not differentially expressed between the two groups No. PAPP-A2 mrna copies /10 4 REF mrna copies.016
17 SUMMARY OAR12: an interesting candidate Investigating the functional properties of this region Conclusion & perspectives.017
18 CONCLUSION & PERSPECTIVES Take home message A 4SNP region in OAR12 was associated to sheep resistance This functional study seems to confirm its role This region seems to impact worms fertility (1 σp) This region was associated to a Th-2 biased over-expression Perspectives Refine : association analysis in Martinik and Romane sheep Investigating the host-nematode system as a whole.018
19 ACKNOWLEDGEMENTS Supervisors Pr. P. Jacquiet Dr. C. Moreno Quantitative genetics S.C. Bishop J.M. Elsen A. Legarra O. Filangi Parasitology J.-P. Bergeaud C. Grisez E. Liénard F. Prévot Lab Farm interface D. François J. Ruesche Experimental farms staff M. Aletru Y. Bourdillon F. Bouvier T. Fassier D. Marcon J-C. Thouly J. L. Weisbecker Financial support Thank you for your attention G. SALLĒ / Functional study of a QTL affecting resistance to H. contortus.019 EAAP 2013, Nantes, France
20 .020
21 Merging knowledge together Positional knowledge Regions involved No gene FEC1 FEC2 OAR12 Differential gene expression (Liénard et al., WAAVP 2011) Breed comparison Control and 8 dpi Tissue samples from abomasum and lymph nodes Comparison of the gene expression of ~15,000 genes (microarray) Uninfected control Infected 8 dpi
22 Merging knowledge together Uninfected control Infected 8 dpi Gene network analysis 157 genes with fold change > 1.5 FEC1 OAR12 Gene set FEC2 Significant QTL Annotated genes contained within LRTmax position +/- 4 Mbp Networks
23 Results of the bioinformatic approach Within the networks related to haemonchosis: a high number of genes mapped on OAR12 No. genes involved in immunity/hemostasis/plc-a2 activity No. genes OAR
24 Association analysis & breeds Due to the two pure breeds histories, SNP alleles may not mirror the same QTL alleles * Relationship between r of the Martinik breed and the Romane breed (0-10 Kb) m Q* n M Q* N M q N M Q* N * The mutation can have appeared after breeds diverged
25 Population genetics input Different environments different selection pressure Stronger selection pressure due to GIN in MBB sheep Is there any gene that has been selected in the MBB sheep and not in the RMN breed?
26 Sweep detection In collaboration with S. Boitard Additional genotyping of MBB and RMN individuals One suggestive sweep at 42 M Fitting the sweep genotype in the QTL detection model erodes the QTL profile Classical model Sweep as a fixed effect
Phenotyping and selecting for genetic resistance to gastro-intestinal parasites in sheep: the case of the Manech French dairy sheep breed
Phenotyping and selecting for genetic resistance to gastro-intestinal parasites in sheep: the case of the Manech French dairy sheep breed JM. Astruc *, F. Fidelle, C. Grisez, F. Prévot, S. Aguerre, C.
More informationGenomic selection in French dairy sheep: main results and design to implement genomic breeding schemes
Genomic selection in French dairy sheep: main results and design to implement genomic breeding schemes F. Barillet *, J.M. Astruc, G. Baloche, D. Buisson, G. lagriffoul et al. * * INRA - Toulouse, France
More informationParasite Control on Organic Sheep Farms in Ontario
Parasite Control on Organic Sheep Farms in Ontario Dr. Laura C. Falzon PhD candidate, Department of Population Medicine, University of Guelph (some slides courtesy of Dr. Andrew Peregrine and Dr. Paula
More informationGenome-wide association analysis of resistance to gastro-intestinal parasites in dairy sheep
Genome-wide association analysis of resistance to gastro-intestinal parasites in dairy sheep S. Casu 1, M.G. Usai 1 S. Sechi 1, M. Casula 1, G.B. Congiu 1, S. Miari 1, G. Mulas 1, S. Salaris 1, T. Sechi
More informationCLUSTERING AND GENETIC ANALYSIS OF BODY RESERVES CHANGES THROUGHOUT PRODUCTIVE CYCLES IN MEAT SHEEP
CLUSTERING AND GENETIC ANALYSIS OF BODY RESERVES CHANGES THROUGHOUT PRODUCTIVE CYCLES IN MEAT SHEEP MACE Tiphaine 1, Gonzalez-Garcia E. 2, Carriere F. 3, Douls S. 3, Foulquié D. 3, Robert-Granié C. 1,
More informationGetting better at collecting what is required. George Cullimore - Performance Recorded Lleyn Breeders
Getting better at collecting what is required George Cullimore - Performance Recorded Lleyn Breeders Who are we? A group of like minded Lleyn breeders recording with Signet Formed in 2013 we now have about
More informationLarge Animal Topics in Parasitology for the Veterinary Technician Jason Roberts, DVM This presentation is designed to review the value veterinary
Large Animal Topics in Parasitology for the Veterinary Technician Jason Roberts, DVM This presentation is designed to review the value veterinary technicians can add to mixed or large animal practices
More informationEFFECT OF SERICEA LESPEDEZA HAY ON GASTROINTESTINAL NEMATODE INFECTION IN GOATS
EFFECT OF SERICEA LESPEDEZA HAY ON GASTROINTESTINAL NEMATODE INFECTION IN GOATS G.S. Dykes, T.H. Terrill, S.A. Shaik, J.E. Miller, B. Kouakou, G. Karnian, J.M. Burke, R. M. Kaplan, and J.A. Mosjidis1 Abstract
More informationGenotypes of Cornel Dorset and Dorset Crosses Compared with Romneys for Melatonin Receptor 1a
Genotypes of Cornell Dorset and Dorset Crosses Compared with Romneys for Melatonin Receptor 1a By Christian Posbergh Cornell Undergraduate Honor Student, Dept. Animal Science Abstract: Sheep are known
More informationBreeding for Meat Sheep in France
Breeding for Meat Sheep in France Valérie LOYWYCK, Agathe CHEYPE, Laurence TIPHINE, Jean-Michel ASTRUC 42nd ICAR Conference, Auckland (New Zealand) Workshop: Identification, Meat & Reproduction Recording
More informationHow to accelerate genetic gain in sheep?
How to accelerate genetic gain in sheep? N Mc Hugh 1, A. O Brien 1, F. McGovern 1, E. Wall 2, T. Pabiou 2, K. McDermott 2, and D. Berry 1 1 Teagasc, Moorepark, Ireland & 2 Sheep Ireland Sheep Breeders
More informationTACKLING THE PARASITOLOGICAL CHALLENGES ARISING FROM ORGANIC FARMING PRACTICES
TACKLING THE PARASITOLOGICAL CHALLENGES ARISING FROM ORGANIC FARMING PRACTICES Spiridoula Athanasiadou, ProPara coordinator Leading the way in Agriculture and Rural Research, Education and Consulting ProPara
More informationInnovating sheep genetics
27 July 2018 Rabobank Industry Innovator Presentation Innovating sheep genetics Derek Daniell Masterton Strong base Family of innovators Grandfather, 1913 51 survived WW1, slump, WW2, pushed aerial topdressing
More informationEvolution in dogs. Megan Elmore CS374 11/16/2010. (thanks to Dan Newburger for many slides' content)
Evolution in dogs Megan Elmore CS374 11/16/2010 (thanks to Dan Newburger for many slides' content) Papers for today Vonholdt BM et al (2010). Genome-wide SNP and haplotype analyses reveal a rich history
More informationVirtual Lab: Sex-Linked Traits Worksheet. 1. Please make sure you have read through all of the information in the
Virtual Lab: Sex-Linked Traits Worksheet 1. Please make sure you have read through all of the information in the Questions and Information areas. If you come upon terms that are unfamiliar to you, please
More informationINTERNAL PARASITES OF SHEEP AND GOATS
7 INTERNAL PARASITES OF SHEEP AND GOATS These diseases are known to occur in Afghanistan. 1. Definition Parasitism and gastrointestinal nematode parasitism in particular, is arguably the most serious constraint
More informationSHEEP PARASITE MANAGEMENT
SHEEP PARASITE MANAGEMENT Past, Present and Future Scott Bowdridge, Ph.D. West Virginia University Division of Animal and Nutritional Sciences How does drug-resistance develop? Assumption: All de-wormers
More informationFaculty of Agricultural and Nutritional Science
Faculty of Agricultural and Nutritional Science Christian-Albrechts-University Kiel Institute of Animal Breeding and Husbandry Genome-wide association studies for production traits in pooled pig FF 2 designs
More informationTreatment Strategies to control Parasitic Roundworms In Cattle
Treatment Strategies to control Parasitic Roundworms In Cattle Dave Bartley Which roundworms are most likely to cause problems? Scientific name Common name Disease Ostertagia ostertagi Brown stomach worm
More informationMeta-analysis of the parasitic phase traits of Haemonchus contortus infection in sheep
Saccareau et al. Parasites & Vectors (2017) 10:201 DOI 10.1186/s13071-017-2131-7 RESEARCH Open Access Meta-analysis of the parasitic phase traits of Haemonchus contortus infection in sheep Mathilde Saccareau
More informationGenes controlling resistance to gastrointestinal nematodes in ruminants
Animal Science Papers and Reports vol. 22 (2004) no. 1, 135-139 Institute of Genetics and Animal Breeding, Jastrzębiec, Poland Presented at the Conference Gene polymorphisms affecting health and production
More informationHaemonchus contortus (Nematoda: Trichostrongylidae) infection in lambs elicits an unequivocal Th2 immune response
Haemonchus contortus (Nematoda: Trichostrongylidae) infection in lambs elicits an unequivocal Th2 immune response Caroline Lacroux, Thi Hai Chi Nguyen, Olivier Andreoletti, Françoise Prevot, Christelle
More informationSustainable Integrated Parasite Management (sipm)
Sustainable Integrated Parasite Management (sipm) The goal of a parasite control program is to control the parasites on a farm to a level which has minimal effect on animal health and productivity without
More informationThe genetic basis of breed diversification: signatures of selection in pig breeds
The genetic basis of breed diversification: signatures of selection in pig breeds Samantha Wilkinson Lu ZH, Megens H-J, Archibald AL, Haley CS, Jackson IJ, Groenen MAM, Crooijmans RP, Ogden R, Wiener P
More informationQuantitative trait loci for resistance to Haemonchus contortus artificial challenge in Red Maasai and Dorper sheep of East Africa
doi: 10.1111/j.1365-2052.2012.02401.x Quantitative trait loci for resistance to Haemonchus contortus artificial challenge in Red Maasai and Dorper sheep of East Africa K. Marshall*, J. M. Mugambi, S. Nagda*,
More information8/23/2018. Gastrointestinal Parasites. Gastrointestinal Parasites. Haemonchus contortus or Barber Pole Worm. Outline
8/23/218 Genetic Selection for Parasite Management Texas Sheep and Goat Expo, San Angelo, TX, August 18, 217 Joan M Burke Research Animal Scientist USDA, ARS, Dale Bumpers Small Farms Research Center Booneville,
More informationPARASITOLOGY IN 2020 Where will we stand? EU Framework Programmes PARASOL & GLOWORM & PARAVAC
PARASITOLOGY IN 2020 Where will we stand? EU Framework Programmes PARASOL & GLOWORM & PARAVAC All grazing ruminants are infected with helminths, however, only some need to be treated Production diseases
More informationVirginia Journal of Science, Vol. 61, No. 1, 2010
Virginia Journal of Science Volume 61, Number 1& 2 Spring/Summer 2010 Garlic as an Alternative Anthelmintic in Sheep A. Curry and B. D. Whitaker 1 Agriculture Program, Ferrum College, Ferrum VA, 24088,
More informationEdinburgh Research Explorer
Edinburgh Research Explorer Introgression of Ivermectin Resistance Genes into a Susceptible Haemonchus contortus Strain by Multiple Backcrossing Citation for published version: Redman, E, Sargison, N,
More informationJerry and I am a NGS addict
Introduction Identification and Management of Loss of Function Alleles Impacting Fertility L1 Dominette 01449 Jerry and I am a NGS addict Jerry Taylor taylorjerr@missouri.edu University of Missouri 2014
More informationChronMast - a model to study functional genetic variation of mastitis susceptibility
ChronMast - a model to study functional genetic variation of mastitis susceptibility M. Meyerholz 1,2, A. Heimes 3, J. Brodhagen 3, L. Rohmeier 2, T. Eickhoff 1, S. Jander 1, A. Hülsebusch 1, R. Weikard
More informationMiniature Schnauzer Annual Breed Health Report 2016
Miniature Schnauzer Annual Breed Health Report 2016 This form should be completed by your Breed Health Co-ordinator and submitted via email to the Kennel Club s Health Team (email address tbc). Section
More informationManhattan and quantile-quantile plots (with inflation factors, λ) for across-breed disease phenotypes A) CCLD B)
Supplementary Figure 1: Non-significant disease GWAS results. Manhattan and quantile-quantile plots (with inflation factors, λ) for across-breed disease phenotypes A) CCLD B) lymphoma C) PSVA D) MCT E)
More informationSheep Breeding. Genetic improvement in a flock depends. Heritability, EBVs, EPDs and the NSIP Debra K. Aaron, Animal and Food Sciences
ASC-222 Sheep Breeding Heritability, EBVs, EPDs and the NSIP Debra K. Aaron, Animal and Food Sciences Genetic improvement in a flock depends on the producer s ability to select breeding sheep that are
More informationDuddingtonia flagrans What is it?
Duddingtonia flagrans What is it? A natural strain of fungus isolated from the environment (Australia, early 1990s) Found around the world Application as a biological control for larvae of parasitic worms
More informationSustainable Worm Control Strategies for Sheep. LSSC Ltd
Sustainable Worm Control Strategies for Sheep LSSC Ltd Sustainable Worm Control Strategies for Sheep This slide show has been made available by SCOPS SCOPS is an industry-wide initiative including representation
More informationWorksheet for Morgan/Carter Laboratory #9 Mendelian Genetics II: Drosophila
Worksheet for Morgan/Carter Laboratory #9 Mendelian Genetics II: Drosophila Ex. 9-1: ESTABLISHING THE ENZYME REACTION CONTROLS Propose a hypothesis about AO activity in flies from vial 1a and flies from
More informationLevel 2 Biology, 2015
91157 911570 2SUPERVISOR S Level 2 Biology, 2015 91157 Demonstrate understanding of genetic variation and change 9.30 a.m. Monday 16 November 2015 Credits: Four Achievement Achievement with Merit Achievement
More informationDairy goat farming in Australia: current challenges and future developments
Dairy goat farming in Australia: current challenges and future developments Pietro Celi (DVM, PhD) & Peter White (BVSc, PhD) Faculty of Veterinary Science, University of Sydney 1 Feral Goats 2 Meat Goats
More informationBiology 164 Laboratory
Biology 164 Laboratory CATLAB: Computer Model for Inheritance of Coat and Tail Characteristics in Domestic Cats (Based on simulation developed by Judith Kinnear, University of Sydney, NSW, Australia) Introduction
More informationBi156 Lecture 1/13/12. Dog Genetics
Bi156 Lecture 1/13/12 Dog Genetics The radiation of the family Canidae occurred about 100 million years ago. Dogs are most closely related to wolves, from which they diverged through domestication about
More informationEcology/Physiology Workgroup. Nematode Parasites and Grazing Research
Ecology/Physiology Workgroup Nematode Parasites and Grazing Research James E. Miller 1, John A. Stuedemann 2 and Thomas H. Terrill 3 1 Parasitologist, Department of Pathobiological Sciences, Department
More informationStation 1. Using the cards, match the vocabulary word with its definition. If there are any words you do not know, write them down if you have time!
Station 1 Using the cards, match the vocabulary word with its definition. If there are any words you do not know, write them down if you have time! Station 2 Answer the following questions on a separate
More informationMonitoring methods and systems
Monitoring methods and systems Georg von Samson-Himmelstjerna, Jürgen Krücken Institute for Parasitology and Tropical Veterinary Medicine Freie Universität Berlin What suitable and validated tools/tests
More informationFruit Fly Exercise 2 - Level 2
Fruit Fly Exercise 2 - Level 2 Description of In this exercise you will use, a software tool that simulates mating experiments, to analyze the nature and mode of inheritance of specific genetic traits.
More informationEvaluation of terminal sire breeds in hair sheep production systems
217 Virginia Shepherd's Symposium Evaluation of terminal sire breeds in hair sheep production systems A.R. Weaver, D.L. Wright, M.A. McCann, D.R. Notter, A.M. Zajac, S.A. Bowdridge, S.P. Greiner Goal:
More informationparasitic nematodes in domestic ruminants
Functional Ecology 2001 Massive use of chemotherapy influences life traits of Blackwell Science, Ltd parasitic nematodes in domestic ruminants V. LEIGNEL and J. CABARET INRA, PAP, 37380 Nouzilly, France
More informationChapter 11. Human Genetic Analysis
Chapter 11 Human Genetic Analysis 1. Complex inheritance of traits does not follow inheritance patterns described by Mendel. 2. Many traits result from alleles with a range of dominance, rather than a
More informationThe Eye of the Farmer and Detection of Animals in Need of Anthelmintic Treatment in Organic Meat Sheep Flocks
2 The Open Veterinary Science Journal, 2011, 5, (Suppl 1: M2) 2-6 Open Access The Eye of the Farmer and Detection of Animals in Need of Anthelmintic Treatment in Organic Meat Sheep Flocks M. Bouilhol 1,
More informationIndependent Practice: Red throated booby bird R = red throat r = white throat. 1. Cross RR with rr. 2. Cross Rr with RR.
Using Punnett Squares (Use with the Weblink Baby Steps Through Punnett Squares. ) Guided Practice: T = tall t = short Independent Practice: Red throated booby bird R = red throat r = white throat 1. Cross
More informationMendelian Genetics Problem Set
Mendelian Genetics Problem Set Name: Biology 105 Principles of Biology Fall 2003 These problem sets are due at the beginning of your lab class the week of 11/10/03 Before beginning the assigned problem
More informationImproving lamb survival with calmer sheep
Postgraduate Showcase 27 Frontiers in Agriculture Improving lamb survival with calmer sheep Sam Bickell BSc (Animal Science) Animal Production Systems Background Loss of 1 million lambs per year 4-6% die
More informationThe Use of Vaccine Programmes in Livestock Systems
The Use of Vaccine Programmes in Livestock Systems Alasdair Nisbet, Vaccines, Moredun Research Institute www.moredun.org.uk Moredun Research Institute Vaccines Pillar Viruses, Bacteria and Parasites Host-pathogen
More informationAKC Canine Health Foundation Grant Updates: Research Currently Being Sponsored By The Vizsla Club of America Welfare Foundation
AKC Canine Health Foundation Grant Updates: Research Currently Being Sponsored By The Vizsla Club of America Welfare Foundation GRANT PROGRESS REPORT REVIEW Grant: 00748: SNP Association Mapping for Canine
More informationGenetic approaches to improving lamb survival under extensive field conditions
Genetic approaches to improving lamb survival under extensive field conditions Forbes Brien University of Adelaide and Mark Young Beef + Lamb New Zealand Genetics EAAP 16 Abstract Number 24225 Introduction
More informationPhenotypic and Genetic Variation in Rapid Cycling Brassica Parts III & IV
1 Phenotypic and Genetic Variation in Rapid Cycling Brassica Parts III & IV Objective: During this part of the Brassica lab, you will be preparing to breed two populations of plants. Both will be considered
More informationPrevalence and burden of helminths in laying hens kept in free range systems
Prevalence and burden of helminths in laying hens kept in free range systems F. Kaufmann and M. Gauly Department of Animal Science, University of Goettingen Introduction Development of laying hen husbandry
More informationImportance of docility
Recent Developments in Selection for Docility in Ireland National Seminar on Strategies for improving Safety with Cattle Ross Evans ICBF 23 rd November 2010 Teagasc Health & Safety Conference Grange Importance
More informationBiology 3201 Sex Linked Review Mr.Gillam Name:
Biology 3201 Sex Linked Review Mr.Gillam Name: A female has the chromosomes XX, while a male has the chromosomes XY. In sex-linked inheritance the genes are carried on the chromosome and as a rule and
More informationGENETIC IMPROVEMENT O F LITTER SIZE IN SHEEP
GENETIC IMPROVEMENT O F LITTER SIZE IN SHEEP J.M. Elsen1, L. Bodin1, D. Francois1, J.P. Poivey1 and J Teyssier2 1INRA Station d'amdlioration G6n6tique des Animaux. BP27-31326 Castanet Tolosan - FRANCE
More informationAKC Bearded Collie Stud Book & Genetic Diversity Analysis Jerold S Bell DVM Cummings School of Veterinary Medicine at Tufts University
AKC Bearded Collie Stud Book & Genetic Diversity Analysis Jerold S Bell DVM Cummings School of Veterinary Medicine at Tufts University (February 2017) Table of Contents Breed Development... 2 Founders...
More informationHealthy and Contented Sheep
Healthy and Contented Sheep Associate Professor Bruce Allworth Fred Morley Centre PRESENTERS LOGO Overview Animal health is important $$ Management /season interacts with health Key issues: perinatal lamb
More informationGenetics Problem Set
AP Biology - Unit 6: Patterns of Inheritance Name: Genetics Problem Set Independent Assortment Problems 1. One gene has alleles A and a. Another has alleles B and b. For each genotype listed, what type(s)
More information7. Describe the following with words and give an example: Heterozygous, homozygous recessive, homozygous dominant
Name: Genetics UNIT EXAM Review Below are review questions for each of the 5 learning goals we have addressed during this unit. This is the majority of the science content we covered. However, as a disclaimer
More informationHealth traits and their role for sustainability improvement of dairy production
S20 (abstract no. 18857) IT-Solutions for Animal Production 65 th EAAP Annual Meeting, 25-29 August 2014, Copenhagen / Denmark Health traits and their role for sustainability improvement of dairy production
More informationPhenotype Observed Expected (O-E) 2 (O-E) 2 /E dotted yellow solid yellow dotted blue solid blue
1. (30 pts) A tropical fish breeder for the local pet store is interested in creating a new type of fancy tropical fish. She observes consistent patterns of inheritance for the following traits: P 1 :
More informationLike to see more lambs?
Like to see more lambs? Ovastim can help you increase your profitability The sale of lambs constitutes 7% of gross income in second cross lamb enterprises, and over 5% of gross income in first cross enterprises
More informationSheep Infection by Haemonchus Species: Effect on Haematocrit and Evaluation of the FAMACHA Method in Arsi Negele District, Oromia, Ethiopia
Animal and Veterinary Sciences 2015; 3(2): 74-79 Published online April 13, 2015 (http://www.sciencepublishinggroup.com/j/avs) doi: 10.11648/j.avs.20150302.17 ISSN: 2328-5842 (Print); ISSN: 2328-5850 (Online)
More informationFeeding strategy of Lacaune dairy sheep: Ewes fed in group according to milk yield
Feeding strategy of Lacaune dairy sheep: Ewes fed in group according to milk yield Hassoun P. 1, Hardy A. 2, Tesnière A. 1, Legarto J. 3, De Boissieu C. 3 1 INRA UMR Selmet Montpellier; 2 Lycée Agricole
More informationOpinion of the Scientific Panel on Biological Hazards of the European Food Safety Authority on:
Opinion of the Scientific Panel on Biological Hazards of the European Food Safety Authority on: A quantitative assessment of risk posed to humans by tissues of small ruminants in case BSE is present in
More informationPLEASE PUT YOUR NAME ON ALL PAGES, SINCE THEY WILL BE SEPARATED DURING GRADING.
MIDTERM EXAM 1 100 points total (6 questions) 8 pages PLEASE PUT YOUR NAME ON ALL PAGES, SINCE THEY WILL BE SEPARATED DURING GRADING. PLEASE NOTE: YOU MUST ANSWER QUESTIONS 1-4 AND EITHER QUESTION 5 OR
More informationA Field Study on Efficacy of Albendazole (Albezol ) Against Gastro-intestinal Nematodes in Ruminants
Kasetsart J. (Nat. Sci.) 39 : 647-651 (25) A Field Study on Efficacy of Albendazole (Albezol ) Against Gastro-intestinal Nematodes in Ruminants Theera Rukkwamsuk 1, Anawat Sangmalee 1, Korawich Anukoolwuttipong
More informationInside This Issue. BEYOND numbers. Small Ruminant
S P R I N G 2 0 1 3 Small Ruminant Control of Gastrointestinal Parasites in the 21st Century Part II: We are losing the war now what? Joseph McCoy, DVM, Diplomate ACVP Inside This Issue Control of Gastrointestinal
More informationPrevalence of Gastrointestinal Parasite in Goats in Shillong, Meghalaya, India
Article ID: WMC00777 ISSN 2046-1690 Prevalence of Gastrointestinal Parasite in Goats in Shillong, Meghalaya, India Author(s):Dr. Subhasish Bandyopadhyay, Mrs. Pallabi Devi, Dr. Asit Bera, Dr. Samiran Bandyopadhyay,
More informationOpen Access RESEARCH ARTICLE
https://doi.org/10.1186/s13567-018-0534-x RESEARCH ARTICLE Open Access Exploring the mechanisms of resistance to Teladorsagia circumcincta infection in sheep through transcriptome analysis of abomasal
More informationPromoting One Health : the international perspective OIE
Promoting One Health : the international perspective OIE Integrating Animal Health & Public Health: Antimicrobial Resistance SADC SPS Training Workshop (Animal Health) 29-31 January 2014 Gaborone, Botwana
More informationWorms, Sheep & Goats, & Copper Oxide Wire Particles. Dr. Dwight Bowman
Worms, Sheep & Goats, & Copper Oxide Wire Particles Dr. Dwight Bowman Haemonchus Major problem in sheep Adults in abomasum of Adults 1 to 3 cm long males red, female - white ovaries wrapped around intestine
More informationhusband P, R, or?: _? P P R P_ (a). What is the genotype of the female in generation 2. Show the arrangement of alleles on the X- chromosomes below.
IDTER EXA 1 100 points total (6 questions) Problem 1. (20 points) In this pedigree, colorblindness is represented by horizontal hatching, and is determined by an X-linked recessive gene (g); the dominant
More informationPutting Science into Animal Science Projects. Area: Using Genetics (advanced members) Activity: Eradicate Scrapie in Sheep through Genetic Selection
Putting Science into Animal Science Projects Area: Using Genetics (advanced members) Activity: Eradicate Scrapie in Sheep through Genetic Selection Goal: Provide advanced members with the information and
More informationEconomically important trait. Increased demand: Decreased supply. Sheep milk cheese. 2007: $2.9 million for milk production (Shiflett, 2008)
Genetic Markers for Milk Production Raluca Mateescu, OklahomaStateUniversity Michael Thonney, Cornell University Milk production & Sheep Industry Economically important trait 2007: $2.9 million for milk
More informationSheep Breeding in Norway
Sheep Breeding in Norway Sheep Breeders Round Table 2015 Thor Blichfeldt Ron Lewis Director of Breeding Professor, University of Nebraska-Lincoln The Norwegian Association of Sheep and Goat Breeders (NSG)
More information+ Karyotypes. Does it look like this in the cell?
+ Human Heredity + Karyotypes A genome is the full set of genetic information that an organism carries in its DNA. Karyotype: Shows the complete diploid set of chromosomes grouped together in pairs, arranged
More informationNo tail (Manx) is a dominant trait and its allele is represented by M The presence of a tail is recessive and its allele is represented by m
Lab #4: Extensions to Mendelian Genetics Exercise #1 In this exercise you will be working with the Manx phenotype. This phenotype involves the presence or absence of a tail. The Manx phenotype is controlled
More informationGenes What are they good for? STUDENT HANDOUT. Module 4
Genes What are they good for? Module 4 Genetics for Kids: Module 4 Genes What are they good for? Part I: Introduction Genes are sequences of DNA that contain instructions that determine the physical traits
More informationQuestion 3 (30 points)
Question 3 (30 points) You hope to use your hard-won 7.014 knowledge to make some extra cash over the summer, so you adopt two Chinchillas to start a Chinchilla breeding business. Your Chinchillas are
More informationParasites in Sheep Flocks
Parasites in Sheep Flocks 1 WHAT IS NEW IN PARASITE CONTROL FOR SHEEP FLOCKS? Drew E. Hunnisett, DVM Honeywood and Warder Veterinary Services 132 Commerce Park Drive, Unit N Barrie, Ontario L4N 8W8 705
More informationMonohybrid Cross Video Review
Name: Period: Monohybrid Cross Video Review 1. What is the name of the little boxes used in order to predict offspring without having to breed? 2. Define Punnett Square: 3. Define a monohybrid cross: 4.
More informationMendel s Laws: Their Application to Solving Genetics Problem
Solving Genetics Problems Page 1 Mendel s Laws: Their Application to Solving Genetics Problem Objectives This lab activity is designed to teach students how to solve classic genetics problems using Mendel
More informationGENETIC ANALYSIS REPORT
GENETIC ANALYSIS REPORT OWNER S DETAILS Maria Daniels Bispberg 21 Säter 78390 SE ANIMAL S DETAILS Registered Name: Chelone Il Guardiano*IT Pet Name: Chelone Registration Number: SVEARK LO 343083 Breed:
More informationCLINICAL STUDY OF ACUTE HAEMONCHOSIS IN LAMBS
Trakia Journal of Sciences, No 1, pp 74-78, 2017 Copyright 2017 Trakia University Available online at: http://www.uni-sz.bg ISSN 1313-7050 (print) ISSN 1313-3551 (online) doi:10.15547/tjs.2017.01.012 Original
More informationPatterns of Inheritance. What are the different ways traits can be inherited?
Patterns of Inheritance What are the different ways traits can be inherited? Review: Patterns of Inheritance we know already 1. Autosomal dominant: If an individual is heterozygous, only one allele is
More informationCan animal breeding improve domestic animals experiences?
Can animal breeding improve domestic animals experiences? Susanne Hermesch Susanne.Hermesch@une.edu.au Presented at CSIRO and AGBU seminar Armidale 26 September 2013 Acknowledgements AGBU Rob Banks Daniel
More informationLevel 1 Science, 2011
90948 909480 1SUPERVISOR S Level 1 Science, 2011 90948 Demonstrate understanding of biological ideas relating to genetic variation 9.30 am onday Monday 21 November 2011 Credits: Four Achievement Achievement
More informationThe purpose of this lab was to examine inheritance patters in cats through a
Abstract The purpose of this lab was to examine inheritance patters in cats through a computer program called Catlab. Two specific questions were asked. What is the inheritance mechanism for a black verses
More informationPunnett Square Review
Punnett Square Review Complete each of the following problems to practice the 4 different types of crosses 1. In peas, yellow color (G) is dominant to green (g). What are the possible genotypes and phenotypes
More informationSTUDIES ON HAEMONCHUS CONTORTUS. IV. THE EFFECT OF TRICHOSTRON GYLUS AXEl AND OSTERTAGIA CIRCUMCINCTA ON CHALLENGE WITH H.
Onderstepoort J. vet. Res., 48, 229-234 (1981) STUDES ON HAEMONCHUS CONTORTUS. V. THE EFFECT OF TRCHOSTRON GYLUS AXEl AND OSTERTAGA CRCUMCNCTA ON CHALLENGE WTH H. CONTORTUS R. K. RENECKE, CHRSTEL BRUCKNER(!)
More informationAS91157 Demonstrate understanding of genetic variation and change. Punnett Squares
AS91157 Demonstrate understanding of genetic variation and change (2017,1) PIGEON GENETICS Punnett Squares Pigeon wing pattern and leg feathering both show complete dominance. The bar wing allele (B) is
More informationA New Index for Mastitis Resistance
A New Index for Mastitis Resistance F. Miglior, * A. Koeck, * G. Kistemaker and B.J. Van Doormaal * Centre for Genetic Improvement of Livestock, University of Guelph Canadian Dairy Network Guelph, Ontario,
More informationOIE activities on rabies: PVS, vaccine banks and the OIE twinning
Dr Gardner Murray, Special Adviser World Organisation for Animal Health (OIE) OIE activities on rabies: PVS, vaccine banks and the OIE twinning OIE Global Conference on Rabies Control: with the support
More informationDO NOT WRITE ON THIS TEST Unit 6 Assessment Genetics Objective 3.2.2
DO NOT WRITE ON THIS TEST Unit 6 Assessment Objective 3.2.2 Vocabulary Matching + 1 point each 1. dominant 2. recessive 3. genotype 4. phenotype 5. heterozygous 6. homozygous 7. incomplete dominance 8.
More information