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1 Curriculum vitae PERSONAL INFORMATION Lucie Pokludová WORK EXPERIENCE January 1993 November 1998 microbiologist: diagnostic and analytical microbiology Checking of the microbiological quality of VMPs Microbiological analytics: efficacy of antimicrobiologicals December 1998 August 2000 maternity leave + part time job maternity leave, stay in Germany, language training external work on translation of documents ref VMPs (pre-eu stage of CZ) September 2000 September 2002 microbiologist: diagnostic and analytical microbiology Checking of the microbiological quality of VMPs Microbiological analytics: efficacy of antimicrobiologicals Disinfectants efficacy testing October 2002 August 2005 maternity leave + part time job maternity leave Part time job:translations of documents related to antimicrobials, microbiology, veterinary medicinal product authorization September 2005 September 2010 assessor of safety: residues EMA - SWP member October 2010 Present assessor of Efficacy: antimicrobials Assessor of efficacy ESVAC coordinator and national contact point, February 2000 Present external teacher Faculty of Science of masaryk University (Czech Republic) Lectures: Essentials of antimicrobials (classification of ATM, mechanism of action of ATM, AMR, spectrum of effiacy of diferent classes of ATM (hum and vet medicine), diagnostic + susceptibility testing of ATM, principles of antibiotic policies) November 2017 May 2019 tutor UNE-AENOR-EC (Spain) BTSF - tutor - AMR training course - day 2 tutor (AMU/AMR/EU bodies, Prudent use GLs etc) 20/3/18 European Union, Page 1 / 5

2 EDUCATION AND TRAINING September 1988 June 1993 MSc: microbiology, molecular biology and genetics, general biology Faculty of Science Masaryk University Brno (Czech Republic) microbiology: diagnostic, nalytical microbiology, physiology of bacteria molecular biology and genetics, general biology September 1993 December 1997 PhD: specialised microbiology Faculty of Science Masaryk University Brno (Czech Republic) microbiology, diagnostic microbiology, susceptibility testing, microbiological quality of VMP, computer image analysis used in diagnostic microbiology, computer identification of microorganisms ADDITIONAL INFORMATION Expertise Principal areas of expertise: - Microbiology: Antimicrobials - efficacy, mechanism of action and resistance, antimicrobial resistance, - Safety of residues - Veterinary medicinal products authorization - Diagnostic and analytical microbiology - Computer image analysis in microbiology collection of the data of veterinary antimicrobials sales/use Publications Publications (last 10 years) International Peer Reviewed Journals 2016 Janecko N., Pokludova L., Blahova J., Svobodova Z., Literák I., Implications of fluoroquinolone contamination for the aquatic environment - A review. Environmental Toxicology and Chemistry Journal N. De Briyne, J. Atkinson, L. Pokludová, S. P. Borriello:Antibiotic used most commonly to treat animals in Europe, Veterinary Record, Vet Rec. 2014, May 2014, doi: /vr N. De Briyne, J. Atkinson, L. Pokludová, S. P. Borriello, S. Price: Factors influencing antibiotic prescribing habits and use of sensitivity testing amongst veterinarians in Europe, Veterinary Record, Vet Rec. Sep 25 doi: /vr Ivan Literak, Tomas Reitschmied, Dobroslava Bujnakova, Monika Dolejska, Alois Cizek, Jan Bardon, Lucie Pokludova, Pavel Alexa, Dana Halova, and Ivana Jamborova. Broilers as a Source of Quinolone-Resistant and Extraintestinal Pathogenic Escherichia coli in the Czech Republic Microbial Drug Resistance. February, 19(1): Dolejska M, Jurcickova Z, Literak I, Pokludova L, Bures J, Hera A, Kohoutova L, Smola J, Cizek A. IncN plasmids carrying blactx-m-1 in Escherichia coli isolates on a dairy farm, Veterinary microbiology, 149 () Škaloud J, Pokludová L, Novotná R, Čížek A. Evaluation by Conductance Assay of Shiga Toxin Producing Escherichia coli (STEC) O157 and O26 and their sensitivity to slected disinfectants, Acta 20/3/18 European Union, Page 2 / 5

3 Vet. Brno, 2003, 72: Papers, Posters (last 5 years) 2014 Veterinary antimicrobials: Do risks overwight benefits? Pokludová L., Hera A., Bureš J., SEMINÁR MIKROBIÁLNA REZISTENCIA BEZPEČNOSŤ POTRAVINOVÉHO REŤAZCA, Bratislava, SK, Status of play of the antibiotic policy with respect to veterinary antimicrobials Hera, A., Pokludová,L., Bureš, J., 44. Konference Lenfeldovy a Höklovy dny, Brno, CZ, Veterinary antimicrobials: current status and future possibilities, Pokludová L., INFORMA, Barcelona, ES, Aspects of use of premixes in animals, Pokludová L., Central European Veterinary Congres, Brno, CZ, , Conclusions and perspectives from the surveillance of veterinary antimicrobials consumption CZ, Pokludová L., Central European Veterinary Congres, Brno, CZ, , Activities towards responsible use of veterinary antimicrobials in the Czech Republic, Pokludová,L., Bureš, J, Hera, A., 3rd International Conference, responsible Use of Antibiotics in Animals, Amsterdam, NL, 2014 Hera A., Pokludová L., Bureš J., New requirements on antibiotic policy in veterinary medicine, oral presentation International conferrence XLIII. Lenfeldovy a Höklovy dny,brno, CZ, Pokludová L., Bureš J., Hera A. USE OF ANTIMICROBIALS IN POULTRY IN CZ: CURRENT STATUS AND PERSPECTIVE OF POSSIBLE APPROACHES FOR THE FUTURE, oral presentation, 31st World Veterinary Congress, Prague, 20 Sept Pokludová L., Bureš J., Hera A. TRENDS IN USE OF ANTIMICROBIALS IN PIGS IN THE CZECH REPUBLIC oral presentation, 31st World Veterinary Congress, Prague, 18 Sept Pokludová L. View of the assessor on data requirement for antimicrobials, oral presentation, Regulation of Veterinary Medicines in Europe Evaluating European Regulatory Requirements for Antimicrobials, INFORMA, 9th Annual Conference: Regulation of Veterinary Medicines in Europe, Barcelona, ES ANALYSIS OF THE TRENDS OF SALES AND PRESCRIPTIONS OF CEPHALOSPORINS OF THE 3rd AND 4th GENERATION IN VETERINARY MEDICINE AN EXPERIENCE FROM THE CZECH REPUBLIC Pokludová L., Bureš J., Koutecká L., Kučerová Z., Hera A. Antimicrobial Resistance in Animals and the Environment (ARAE ), Ghent, BE, poster 2012 Pokludová L., System of surveillance of antimicrobials VMPs consumption in CZ and EU (Systém sledování spotřeby VLP v ČR a EU), oral presentation Central European Veterinary Kongres, Brno, CZ, April 2012 Pokludová L., Bureš J., Surveys on prescribing habits, Status of play, further proceeding oral presentation, HMA V- WG on AMR, Copenhagen, DK Pokludová L., Antimicrobial policy options from the perspective of the national competent authority - Experience from CZ, oral presentation, Regulation of Veterinary Medicines in Europe Pre Conference Workshop: Evaluating European Regulatory Requirements for Antimicrobials, INFORMA, 8th Annual Conference: Regulation of Veterinary Medicines in Europe, Barcelona, ES Hera A., Pokludová L., Bureš J., National antibiotic policyof the Czech Republic, oral presentation International conferrence XLIII. Lenfeldovy a Höklovy dny,brno, CZ, /3/18 European Union, Page 3 / 5

4 Pokludová L, Bureš J, Hera A. Analysis of antimicrobials prescribing patterns in dogs in small and mixed animal veterinary practices, conference: Responsible Use of Antibiotics in Animals Bilthoven, NL, poster Pokludová L, Muentener C, Greko C. Antimicrobials for intrammamary use Comparing data from three European countries, 4th Symposium on Antimicrobial Resistance in Animals and the Environment (ARAE ), Tours, FR, poster 2010 Greko C, Pokludova L, Althaus F, Flechtner O, Muentener CR. Sales of antimicrobials for medication of groups of animals in the Czech Republic, Sweden and Switzerland, 2nd ASM Conference on Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens in Animals, Humans and the environment, Toronto, Canada, poster + oral presentation. 2010: Pokludová L, Bureš J. Decisions driving prescription of antimicrobials in practice, Central European Veterinary Congress, Brno, CZ, oral presentation 2009 Pokludová L, Bureš J, Hera A. Evaluation of the sales data of prudent use antimicrobials contained in veterinary medicinal products in the Czech Republic, trends and perspectives, EAVPT 2009, Leipzig, DE, poster 2009: Pokludová L, Jeřábková J, Hera A. Current approaches in use of veterinary medicinal products in poultry breeds, Conferrence of the Czech Association of poultry Medicine, Brno, CZ, oral presentation 2008 Pokludová L, Hera A, Novotná P, Billova V. Antimicrobial Substances in Veterinary Medicine in the Czech Republic, FOURTH AAVM CONFERENCE, PRAGUE, CZ Projects Ministry of Agriculture of the Czech Republic Grant number QJ : Participation at grant: Development and manufacturing of the new sets of MIC testing of antimicrobial substances by standardised microdilution method and the new concept of efficacy evaluation of antimicrobial substances by setting of the MPCs Memberships Tutor at BTSF - AMR training courses (EU, ) ;Member of Scientific Com EAVPT 2018 Wroclav; Activities related to the EU regulatory bodies: Member of ESVAC EAG overal sales; ESVAC EAG stratification of data, AACTING observer 2017; MEMBER OF SAFETY WORKING PARTY EMA CVMP (2007 ) Several co-rapporteurship MEMBER OF EMA ESVAC TCG group (from 2009) Surveillance of veterinary antimicrobials consumption EC Working group on antimicrobials (from 2010) HMA V - AMR: Action plan working group (from 2010) CZ representative in international activities: Codex Alimentarius CCRVDF 2007, 2009 TFAMR Task Force on AMR CZ delegate for the negotiation on certain legal provisions at the EU Council level (Reg. No. 470/2009, 37/2010, Revision of the legal provisions on the veterinary medicinal products and medicated feed (since 2014)); National level: Member of the Working group on Antimicrobials, Ministry of Agriculture of the Czech Republic Acts as a thesis supervisor of the students: The attestation thesis supervisor of the student (Veterinary and Pharmaceutical University, Brno): Amoxicillin residues in sows tissues,, Veronika Střechová, MVD. 20/3/18 European Union, Page 4 / 5

5 The diploma thesis supervisor of the student (Faculty of Science, Brno): Survey of occurrence of ESBL strains in selected species of enterobacteria in cattle farms in the Czech Republic, 2010, Zuzana Jurčíčková, Bc. The bachelor thesis supervisor of the student (Faculty of Science, Brno): Occurrence of methicillin resistant strains of selected species of the genus Staphylococcus presented in veterinary isolates, 2009, Radka Měkutová, The diploma thesis supervisor of the student (Veterinary and Pharmaceutical University,Brno): Monitoring of resistance to selected antimicrobials in isolates from bovine mastits 1996, Martina Pellantová Member of examination committees (Veterinary and Pharmaceutical University, Brno; Faculty of Science, Brno). Other Relevant Information 20/3/18 European Union, Page 5 / 5

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