Use of a Vet Diagnostic Lab as Surge Capacity for CFIA Bovine TB Investigation

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Use of a Vet Diagnostic Lab as Surge Capacity for CFIA Bovine TB Investigation C. Johnson, B. Chelack, A. Trokhymchuk 2017 CAHLN Annual Meeting Guelph, ON 1

Situation 2 Substantial backlog of cases requiring confirmatory testing Untenable economic situation for Alberta/Saskatchewan ranchers with quarantined herds Urgent need to reduce risk of further spread and negative impact on Canadian beef industry Local facilities inadequate to meet demand for carcass inspection and tissue harvest

3 Saskatchewan cattle December 2016

Prairie Diagnostic Services, Inc. (PDS) Independent, non-profit enterprise Funding: 4 Public SK Ministry of Agriculture University of Saskatchewan/Western College of Veterinary Medicine (WCVM) Fee-for-Service Research grants (WCVM, College of Agric., other) Western Canada veterinary practitioners, livestock producers, feed mills, grain exporters, animal health industry

Prairie Diagnostic Services, Inc. (PDS) 5 Mandate: Provide veterinary diagnostic services and animal disease surveillance in support of the SK Ministry of Agriculture Provide veterinary diagnostics, teaching and research support for the Western College of Veterinary Medicine Provide diagnostic services for the veterinary profession, animal health and agriculture industries of western Canada

6 WCVM and PDS, 52 Campus Drive, Saskatoon

7 Necropsy Facility at PDS/WCVM

Challenges Distance: southern AB/SK border to Saskatoon Local transit Transit across campus, past UofS Dairy Barn Euthanasia Off-site location set up with temporary holding pens Timing of euthanasia and delivery to PDS Critical to preserve tissue quality 8

Saskatoon Jenner 9 SE Alberta / SW Saskatchewan to Saskatoon by truck: 450+ km, 4.5-5 hours in good weather

10 University of Saskatchewan teaching and research dairy May 2017

Challenges 11 Planning complexity Minimize disruption to routine diagnostics business, teaching and research Scheduling nightmare Facility Not designed for high throughput post-mortems of heavy cattle Hoist system overuse; use limited to trained operator Lateral recumbency post-mortems only Waste water system

12 Hoist in action Waste water holding tank

Trial Schedule for 10 Weeks of Bovine TB Eradication Project Work as of January 22, 2017 Hydraulic Table 1 Hydraulic Table 2 Pathologist Grad St RVT Pathologist Grad St RVT Support1 sample manager Week 1 Jan-24 Tues AT IK PDS1 KR BW SW PM Anthony P CW Jan-26 Thurs JR IK PDS2 BE HP DMR PM Anthony P LR Jan-27 Fri YH RK PDS3 LH BW UP AT Anthony P LR Week 2 Jan-31 Tues EZ DMR PDS1 KR ES RK AT Anthony P CW 580 week - F. Marron Feb-02 Thurs JR IK PDS2 KR HP SW AT Anthony P LR Feb-03 Fri AT RK PDS3 BE BW??? Anthony P TG Week 3 Feb-07 Tues RAM IK PDS1 LH HP? PM LR TG Feb-09 Thurs JR IK PDS2 KR FS DMR PM LH TG Feb-10 Fri EZ RK PDS3 BE FS UP PM LR CW Week 4 Feb-14 Tues AT DMR PDS1 KR HP RK PM Anthony P TG 580 week - Andy Allen Feb-16 Thurs JR IK PDS2 LH BW SW PM Anthony P LR Feb-17 Fri PDS3 RK PDS3 KR LS GA PM Anthony P?? Week 5 Feb-21 Tues PDS1 IK PDS1 BE BW SW VMC1 Anthony P TG Feb-23 Thurs JR IK PDS2 LH VetPath2?? VMC2 Anthony P LR Feb-24 Fri PDS3 RK PDS3 BE GA UP?? Anthony P CW Week 6 Feb-28 Tues JR DMR PDS1 KR Vet Path1 RK PM Anthony P TG 580 week - H. Philibert Mar-02 Thurs PDS2 IK PDS2 LH VetPath2 SW PM Anthony P?? Mar-03 Fri PDS3 RK PDS3 BE VetPath3 6 PM Anthony P?? Week 7 Mar-07 Tues PDS1 IK PDS1 KR Vet Path1 SW PM Anthony P TG Mar-09 Thurs PDS2 IK PDS2 LH VetPath2?? PM Anthony P LR Mar-10 Fri PDS3 RK PDS3 BE VetPath3 UP PM Anthony P CW Week 8 Mar-14 Tues PDS1 1 PDS1 KR Vet Path1 RK PM Anthony P TG 580 week Mar-16 Thurs PDS2 IK PDS2 LH VetPath2 SW PM Anthony P LR Mar-17 Fri PDS3 RK PDS3 BE VetPath3 6 PM Anthony P?? Week 9 Mar-21 Tues PDS1 IK PDS1 KR Vet Path1 4 PM Anthony P TG Mar-23 Thurs PDS2 IK PDS2 LH VetPath2 SW PM Anthony P LR Mar-24 Fri PDS3 RK PDS3 BE VetPath3 UP?? Anthony P CW Week 10 Mar-28 Tues PDS1 1 PDS1 KR Vet Path1 RK PM Anthony P TG Mar-30 Thurs PDS2 IK PDS2 LH VetPath2 SW PM Anthony P LR Mar-31 Fri PDS3 RK PDS3 BE VetPath3 6 PM Anthony P?? Notes LR off Feb 3 ANP off this wk BE off this wk KR off this wk LR off Mrch 2 & 3 580 Wildlife - must discuss plan with TB Pathologists 60-1/2 days 30 each from PDS and VetPath Grad students 60-1/2 days grad students each provide 1/2 day per week RVT's 60-1/2 days PDS has 3 3 RVT's looking at maybe 2 from VMC and Angie from Vet Path 30 half days from sample manager - could be a tech need to think about EDO's and vacation 13 Bovine TB campaign scheduling

Challenges 14 Biosafety SOPs for zoonotic pathogens (RG3) PPE and respirator mask fit testing Necropsy Facility entry/exit procedures Costs Transportation Feed/labor while in Saskatoon area holding pens Labor (1X, 1.5X, 2X for in-scope; Prof. staff honoraria) PPE Carcass disposal and scheduling challenges

15 PPE for all

Challenges 16 Personnel Exhaustion Exposure risk (TB testing of PDS NF staff) Injury risk Public concerns Animal welfare University herd exposure Media (Global TV News Saskatoon) Weather Extreme cold, snow, ice

Opportunities 17 Teaching Bovine anatomy, pathology, tuberculosis disease & eradication, Skilled labor Anatomic pathology Biosafety training True spirit of volunteerism and giving-back for a good cause Team building (CFIA staff, PDS staff, WCVM faculty, graduate and veterinary students)

18 The A Team at work

Outcomes 19 3 campaigns over 3 months November, December, January Shift from weekends to weekdays Revisited/adjusted planning and costing each campaign 140+ head processed Speed improved significantly over time and with greater skilled/experienced personnel Overall costs covered, with in-kind support donated

Outcomes Limited teaching value (more anatomy than pathology) Students significantly slow-down process, but presence important to WCVM interests Fatigue: physical, mental One minor injury 20

Lymph node identification 101 21 Shower next

Recommendations for the Future 22 Considerations for On-going Use of PDS/WCVM Necropsy Facility Adequate time for planning Availability of trained personnel and facility Avoidance of teaching & research commitments Availability of local cattle holding facility and transit Contracting carcass disposal Build upon prior experience Schedule work primarily on weekdays to manage overtime costs Construct a carcass rail system within the NF

Recommendations for the Future 23 Ideal for surge capacity an available offsite abattoir in Saskatoon area Tap skilled labor at PDS/WCVM Avoid disruption to diagnostics, teaching and research Not encumbered by full NF SOPs Manage euthanasia on-site Carcass rail for more efficient inspection and lymph node harvest

Recognition 24 Lois Ridgway, PDS NF Area Supervisor, for SOP and Biosafety training, for leadership during campaigns, and for photos Kelsey Rokosh, Leah Heasman, Bridget Enright, Anthony Pashovitz, PDS NF Staff, for their tireless efforts and can-do attitudes Brian Chelack, PDS Director of Operations, for leadership, planning, communication and help with sample prep Gail Krohn, Director of Quality Management and Projects, for sample management organization and planning efforts Anatoliy Trokhymchuk for necropsy help and for his suggestion this information might be of interest to other publicly funded veterinary diagnostic labs and the CFIA

Recognition PDS Anatomic Pathologists, for their skilled post-mortems and willingness to endure long days catching up on routine diagnostic cases Brad Blackmore, WCVM Research Farm Manager, for critical local logistics support Dean Douglas Freeman and WCVM/CWHC Faculty, Clinicians, Staff and Students for their unwavering support and skilled anatomic pathology work Dr. Alex McIsaac, Dr. Dennis Will, and CFIA support staff for their oversight and daily guidance from start to finish CFIA for this opportunity to assist in their investigation 25

Thank you! Questions, comments, ideas welcome 26 Saskatchewan spring stripes, somewhere near Saskatoon April 2017