FMM/RAS/298: Strengthening capacities, policies and national action plans on prudent and responsible use of antimicrobials in fisheries Workshop 2 in cooperation with Malaysia Department of Fisheries and INFOFISH 7-9 August 2017, Kuala Lumpur, Malaysia CHINA: Progress report on the aquaculture component of country NAPs on AMR Dr Yuting Deng dengyt@prfri.ac.cn
CONTENTS Disease control in Chinese aquaculture AMR survey in Chinese aquaculture
1. Disease control in Chinese aquaculture Aeromonas Vibrio Streptococcus Bacterial pathogens Ø Have a high adaptability to Edwardsiella Pseudomonas Flavobacterium environmental changes Ø Show a preference for certain species and certain organs
1. Disease control in Chinese aquaculture Immunological control Medical control Ecological control ü ü ü ü Antimicrobial agents Antiparasitic agent Disinfectant Chinese herbal medicines
Disease control in Chinese aquaculture Nine antimicrobial agents were permitted use in Chinese aquaculture. Tetracyclines Doxycycline Quinolones Enrofloxacin Aminoglycosides Amphenicols Neomycin Thiamphenicol Florfenicol Amphenicols Sulfamonomethoxine Sulfanilamide/TMP Sulfamethazine/TMP Sulfamethoxazole/TMP
2008 2015 2017 surveillance program on AMR from animal origin surveillance program on AMR from aquatic animal origin action plan on decreasing AMU in aquaculture
Ø Supported by the project of the Special Fund for Agroscientific Research in the Public Interest (2012~2016), PRFRI, CAFS launched an AMR investigation of Aeromonas from healthy fish and the environment in Guangdong province in the period of 2012~2015.
Established an experimental procedure of micro broth dilution method Bacterial culture Dilution of bacteria solution Dilution of drug Mix with dilution of bacteria solution Incubation of plates Result interpretation
120,0% A.Veronii (56.4%, 522/925) is the predominant species isolated from healthy fish and their breeding environment. Resistance Level (%) 100,0% 80,0% 60,0% 40,0% 20,0% 0,0% NAL CIP ENR FFC CHL DOX NIT SMM SXT CTX AMP NEO AMK GEN Resistance level of 14 antimicrobial agents to Aeromonas isolated from fish and environment during 2012 to 2015
Some resistance genes were identified in Aeromonas from diseased aquatic animals collected at the fisheries hospital, PRFRI from 1995 to 2012. teta qnrs2, aac(6 )-Ib-cr blatem-1, blactx-m-3 ant(3 -)-Ia Deng et al,microbial Drug Resistance,2014
Researches on the resistance mechanism in Aeromonas and Vibrio Ø Ø Ø Ø Quinolones Chloramphenicols Tetracyclines Integron
Quinolones Resistance Mechanism Characterization of quinolone-resistant mutants of A. hydrophila Selected In Vitro isolates from aquatic animals tested strains ATCC7966 GyrA mutation Ser83Ile Selected by NAL: Asp87Tyr Selected by CIP: Ser83Arg ND ND ParC mutation Ser80Ile / Ser80Arg / ND ND ND ND Mutation of GyrA mainly contributes to the quinolone resistance in Aeromonas.
Characterization of 18 PMQR-positive Aeromonas isolates from aquatic animals. PMQR QRDR NO. NAL(mg/L) CIP(mg/L) MIC range MIC 50 MIC 90 MIC range MIC 50 MIC 90 aac(6')-ib-cr + 9 >128 >128 >128 0.5~>8 >8 >8 aac(6')-ib-cr, qnrs2 + 1 >128 / / >8 / / qnrs2 + 1 >128 / / >8 / / qnrs2-7 0.5~4 2 4 0.004~0.5 0.06 0.5
Integron-mediated Resistance Mechanism Characterization of 22 class 1 integron-positive Aeromonas Classes No. isolates from aquatic animals. Viable region (bp) Gene cassettes A 1 >2000 aac(6 )-II+blaOXA-21+cat3 B 12 2000 drfa12+orff+aada2 C 2 2000/ 900/750 drfa12+orff+aada2/ catb3/ arr-3 D 1 2000/ 1700/ 1500 ND E 1 2000/ 800 drfa12+orff+aada2/ drfa17 F 2 1200/ 900 drfa12+orff/ catb3 G 2 800 dfra17 H 1 130 NA Similar ERIC-PCR patterns were found in integronpositive isolates from fish and the environment Deng et al,frontiers in Microbiology,2016