Alkanna hirsutissima Klebsiella pneamoniae Amoxycillin Penicillin Proteus mirabilis Bacillus cereus Ceftriaxone Clindamycin Invivo Proteus mirabilis Klebsiella pneamoniae Proteus mirabilis Bacillus cereus Invivo Klebsiella pneamoniae
The Synergistic Effects of Alkanna hirsutissima Boiled Extract and some Antibiotics on some Bacterial Species in Vitro and in Vivo Prof.Dr. Adeeba Y. Shareef Lect. Nareman S. Nasir Asst. Lect. Talal T. Ali Department of Biology Minstry of Agriculture College of Science Nineveh Mosul University Abstract: The antibacterial effects of boiled aqueos extract of Alkanna hirsutissima on the bacteria, Bacillus cereus, Klebsiella pneamoniae, Proteus mirabilis and the sensitivity of these bacteria to some antibiotics (Penicillin, Amoxycillin,, Clindamycin, Ceftriaxone) were studied. The study also includes the Synergistic effects of Alkanna hirsutissima extract and antibiotics on the bacteria. The results showed that this extract possessed synergistic effects in all of these antibiotics in all the species of bacteria except Klebsiella pneumonia. The study also includes the Invivo synergistic effects of this extract and cefotaxime in the treatment of experimentally infected wounds with within 10 days compared with cefotaxime alone or boiled extract alone which was treated within 17 days. Key words: Synergistic effects, plant extract, Alkanna hirsutissima, antibiotics. Pseudomonas NNLS system(2004) nosocomial infections 2006 Adwan E.coli Pseadomnas aeruginosa Staph.aureu Adwan et.al,2009) Boraginaceae Alkanna hirsutissima Alkannet
Korshid,1998 (Alkannin quinone flavonoids Raphaell kan,1976) Shikonin Invitro Invivo
Alkanna hirsutissima Scientific classification Kingdom : Plantae Order: (unplaced) Family: Boraginaceae Genus: Alkanna Species: Alkanna hirsutissima Proterus mirabilis, Klebsiella pneumonie, Bacillus cereus, Staphycococus aureus (Koneman, et al, 1997) mice (DA2) (CTX) (AM) Amoxocylin Penicillin (p) Ceftriaxone (CRO30) Clindamycin, Bioanalyse Lophylyzer
37 0 24 whatman No.1 Nutrient agar (Bauer et al, 1966) invitro (Wang and Hedin, 1985) 0.01 100 invitro invivo Stepinska etal.,1995 mice 20 15 CTX30 In 20 5 vivo 12 8 50 5 Ketamine Xylazine 70 5 11 10*55 0.5 24 Viable count
1 0.5 9 Control 100 Abo et al.,2004 St.auerus 9 0.5 17 N.agar 1 10 5 Stepinska,et al.,1995 24 37 0 Staph. Penicillin Proteus Bacillus cereus aureus Proteus mirabilis Kleb.pneumoniae mirabilis Amoxocillin Kleb.pneumoniae Ceftriaxone Kleb.pneumoniae Penicillin Staph. aureus (Cohen, 1992) (multiresistant)
(Nascimento, etal. 2000) WHO (Korshid, Proteus mirabilis Bacillus cereus Kleb.pneumoniae Staph. aureus 1998) Amoxycillin Bacillus cereu Amoxocillin Ceftraxone Proteus mirabilis Penicillin Clindamycin Kleb.pneumoniae (Horiuchi etal., (Adwan etal., (Juniior etal., 2005) 2007) 2009) Clindamycin
(Esimon etal., 2006; Aqil etal., 2005) (MIC) (CTX) invivo (CTX)
Penicillin (P10) Amoxicillin (AM10) (CTX30) Clindamycin (DA2) Ceftriaxone (CR30) 13 24 Bacillus cereus 7 24 Proteus mirabilis 10 Klebsiella pneumonia 7 9 Penicillin (P10) Amoxycillin (AM 10) CTX 30) Clindamycin (DA2) Ceftriaxone (CR30) 12 10 12 24 Bacillus cereus 16 8 10 30 10 Proteus mirabilis 8 7 7 7 8 Klebsiella pneumonia 9
200.00 150.00 100.00 50.00 0.00 1.00 3.00 7.00 10.00 14.00 17.00 1
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