تردد بكرتيا الزائفت الزجناريت ملرضى التهاب اجملاري البوليت يف مدينت العمارة Pseudomonas aerugiosa 33 28 2009\4\1-2\1 Pseudomonas aerugiosa Pseudomonas aeruginosa 467
Amoxycillin, Gentamycin, Cefotaxaime, Carbenicillin, Aztreonam, Ciprofloxacin, Piperacillin, Cefazolin. Norfloxacin, Amikacin 6 100 pseudomonas 94 0 aerugiosa Introduction (1) (2) (3) 468
Pseudomonas aerugiosa (4) (5) Nasocomial infection) (6,7) Haemolysin Lipase (8,9) Exotoxin phospholipase C Protease 469
in- vitro (Materials & Methods) (Equipment & Apparatus) - 1 p.aeruginosa (Centrifuge (Autoclave ) (Hot plate magnetic stirrer 37 (Spectrophotometer) ( Incubation) 470
45 (Water distilator) 200 (Electric oven) (Sensitive balance) (Millipore filter unit) (Light microscope) (ph meter) (Culture media) :- 2 471
(11, 10) (Blood base agar) ( Brain heart infusion agar) (Agar-agar medium) (Urea base agar ) (Simon S citrate agar ) (MacConkey agar ) (Muller Hinton agar ) (Nutrient agar &broth ) Difico (U.S.A ) (11,10) 3 (Antibiotic disc) 4 25, Gentamycin 25 Amoxycillin Ciprofloxacin, Carbenicillin, 30 Cefotaxaime, 25 Aztreonam, 30 Cefazolin. 100 472
Piperacillin, Amikacin (ATCC 27853 ) 30 Cefotaxime, 10 Norfloxacin, Standard Strain P. aeruginosa 30 100 30 5 Different materials 6 (11,10) API 20 E Patient s samples 7 2009\4\1-2\1) 10 (11,10) Isolation&Diagnosis Macroscopic appearance) 1 473
(11,10) (Microscopic appearance) :- (Gram (11,10) (Biochemical tests) Urease tests) (Oxidase tests) (Catalases tests) Indol production tests) Methyl red tests) (Voges proskauer tests) (Citrate utilization tests) 2 s stain) 3 (API 20 E Techneqe) :- 4 (Oil ) 37 24 474
Antibiotic susceptibility 12 MacFarland tube 10 1) Disc diffusion tests Non (13,11) (Results&Discussion) lactose fermenter Single (0.7-2.5) Motile Pyocyanin (15,16) Obligately polar flagellum 475
(16,4) P. aeruginosa 1 (API 20 E) 17,16,4 16 33 31 28 5 33 18 51.1 p. %11.4 Nosocomial infection %90-60 aeruginosa 19 476
%9.3 (%8.7) 20 (%8.6) P. aeruginosa 21 P. aeruginosa Cefazolin 2 Amoxycillin ( %90)30 (%100)33 22 477
Cefotaxime Carbencillin 23 (%76) Cefotaxime Gentamycin (BroadSpectrum) (24 p. aeruginosa P. aeruginosa (%94) 31 Aztreonam Amikacin (%88) (%78) 9 2 P. aeruginosa Ciprofloxacin %76 (%90) (%66.4) Aztreonam (%72)23 (%46)15 (%92)30 Amikacin 25 26 p. aeruginosa p. aeruginosa 27 478
(%28.7) (%19.9.) Antibiotic 28 Perfloxacin Ofloxacin Combination P. aeruginosa 1 479
480 2 P. aeruginosa Amoxycillin 25 33 100 Cefazolin 30 3 10 30 90 Carbencillin 100 10 28 23 72 Cefotaxime 30 10 28 23 72 Gentamycin 10 18 54 15 46 Norfloxacin 10 26 78 7 22 Piperacillin 100 27 82 6 18 Ciprofloxacin 25 29 88 4 12 Aztreonam 30 30 92 3 8 Amikacin 30 31 94 2 6
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Frequency of Pseudomonas aerogniosa isolates of urinary tract infection( UTI) patients in Al-Emarha city Ali.A.Saihod Technical Institute - Amara Abstract :- In this study One hundred urine samples obtained from patients suffering from urinary tract infection(uti), whom admitted to AL sadr generl Hospital for the period of 1/2 1/4/2009,in Emarha city. All urine samples were cultured on MacConkey agar and Blood Agar base, then Macroscopic, Miacroscopic appearance and Biochemical tests were done to diagnose the purity isolates of p.aeruginosa, which found with( 33%) while there were (28)isolates obtained. Results of susceptibility to c to antibiotics Amoxycillin, Gentamycin, Cefotaxaime, Carbenicillin, Aztreonam, Ciprofloxacin, Piperacillin, Cefazolin. Norfloxacin, Amikacin ) showed that this bacteria had resistant mode ranged between 6% to 100%, while it was also having wide range of sensitivity ranged between 0% to 94% for Antibiotics which listed. The study should be recommending to use antibiotic combination to reduce antibiotic resistance of bacteria and to treat patients with UTI 485