HVGRC NEWS. President s Letter. A newsletter for members of the Hudson Valley Golden Retriever Club. January March 2015

HVGRC NEWS President s Letter A newsletter for members of the Hudson Valley Golden Retriever Club January March 2015 Another year is upon us and another holiday season is past. I sincerely hope everyone enjoyed the holidays and that the outlook for 2015 is bright with purpose and joy. As I write this, three of our invaluable and long-term members - and our friends - are coming thru the holiday season with serious health issues. Helen Sherman, Paula Strazza and Phyllis Ward have had a very difficult time but we hope the onslaught of flowers and get well cards have brightened their days and put them on the mend. We need you ladies happy and healthy! 2014 was a very successful year for our club. Our Super Specialty weekend was an unqualified success as were both Agility Trials. and the annual Health Clinic. The club did very well financially from these labor-intensive events and our membership is to be applauded for their hard work and dedication. We welcomed two new members into the club, Attila Mocsotar and Jan Anderson and look forward to more new faces in 2015. 2015 promises to be another good year. We are on track with our Health Clinic on January 25th and many thanks go out to Giselle Graham for taking on this responsibility and to Ann Marie Cioffi who is rounding up all the volunteers to make this crazy day a success. Our Super Specialty and Agility Trials are in the planning stages with more details to be announced. We brainstormed meeting ideas at our Holiday Party and hope to kick off the 2015 season with a Fun Field Day in either April or May. Our club is comprised of people and their dogs that are interested in so many different venues and we are striving to meet those needs. We are hoping to have an Agility for Beginners meeting as well as a History of HVGRC. History? Yes, this club does have an amazing history that goes back to some very famous humans and dogs and is fascinating. Other ideas include possible presentations from ICG and Canine Warriors. We are always open to meeting ideas - whether it be topics, speakers etc and look to you for the ideas you would like to [Date] see covered. Attendance at our meetings involves travel and time and we want that to be as informative and fun as possible. Have an idea? Let the Board know. Speaking of the Board, we have an opening for Treasurer due to Alyson Fuge s relocation. A job description has been posted on the HVGRC website if you are interested. Alyson has volunteered to coach the new Treasurer until he/she is up to speed. This position gives you a voice on the Board as well. If you have questions regarding the position, please feel free to contact myself or Alyson. Enjoy your dogs, the cold winter weather. I look forward to seeing you at our meeting this Spring. Bob TABLE OF CONTENTS President s Letter...1 Calendar of Events/Membership News...2 Our Stars...3 Club Activities...4 Deadlines for Next Newsletter...6 HVGRC Officers...7 Bits & Pieces...8 Health Clinic Flyer...9 Special Section: Genetics of SAS...11-18

HVGRC CALENDAR OF COMING EVENTS - 2015 Sun Jan 25 Fri Jan 31 Fri Jan 31 Jan 31-March 31 April 10-12 Sat Feb 14 April 15 June 4-5 Nov 6-8 Annual Health Clinic Chair: Giselle Graham, Co-Chair Ann Marie Cioffi Note: Deadline for enrollment Jan. 15. Check with Giselle to see if space is available End of Grace Period for HVGRC Dues Send forms and checks to Bonnie Oddo Applications due for Breeders Directory Dues payments will incur a $5 late charge After March 31, members must apply for reinstatement Goldens First Agility Trial Chair: Chris Miele Silent Farm, Goshen Entries through PawPrintTrials.com Meet the Breeds at the Javits Center Deadline for application forms for titles and annual trophies HVGRC Specialty Show and Obedience Trial with Rally FDR Park All-Breed Agility Trial Chair: Chris Miele Victoria Farms, Middletown NY Membership News WELCOME NEW MEMBER Attila Mocsotar 232 East 12 th Street, #1B New York, NY 10003 (212) 353-2792 Email: hunattilam@yahoo.com NEW EMAIL ADDRESS Alyson Fuge (Alyson and Dennis will be moving to PA soon) ayfuge@gmail.com SPEEDY RECOVERY Best wishes to three of our members who are coping with serious health problems: Paula Strazza Helen Sherman Phyllis Ward We miss you and hope you will be feeling better soon! 2

!! Our Stars!! Our wonderful Goldens are nothing if not versatile! Read on Bruce and Audrey Coleman travelled to the GRCA National Specialty in Bristol, Tennessee and Ashville, North Carolina and sent this report: We traveled down on Thursday and Friday. Saturday and Sunday were the field events in Bristol. Fanny passed the Senior Hunter test, and then Fanny, Kate and Rocket (at 13 years of age) all passed the Working Certificate test. Monday was a travel and setup day for Obedience. Tuesday and Wednesday was the largest Obedience Trial in AKC history. There were 321 dogs entered in obedience with a total of 520 entries. There were 204 dogs entered in Rally with a total of 248 entries. It took two days to complete this one trial. Tuesday was primarily for the non-regular classes. Vinny qualified in Veteran s Novice Obedience and we fielded a team for the team competition. There were 11 teams entered, mostly dressed in matching clothing/costumes. A team consists of four people and their dogs. We were the Damn Yankees. All four of us, Bruce with Vinny, Audrey with Fanny, and Diane Blackman and Kathy Wager with their dogs gave a spirited performance. When the judge asked if we were ready we responded in unison Batter Up. This is the Praesent vitae nisl. YouTube film of our performance https://www.youtube.com/watchk?v=5t3zzfvscbw Wednesday was the obedience day for the regular obedience classes. Fanny qualified in Open. Thursday was Vinny s opportunity to compete in conformation. He won the *** (Qualified All Age) Field Trial Sweeps! Friday and Saturday we traveled home. Sunday (all day!) was laundry day. A bitch that Karen Fennikoh bred AND co-owns won the Bred-By- Exhibitor class at the National Specialty! Here is the gorgeous photo of Amberglo s Defying Gravity at Maplecroft, AKA Emily, owned by Karen and Bonnie Rubin, and handled by Karen. Their names will be on the GRCA s John Roger Magoffin perpetual trophy, which has been in existence in the GRCA since 1938!!! Talk about being a part of history! Emily Amberglo s Defying Gravity at Maplecroft 3

!! Our Stars cont d!! After In earning hac Qualified habitasse All-Age status platea in August, Sue dictumst. Handelman s Heather earned 3 more Master qualifications in September, at Long Island RFTC, Maryland RC and the Labrador RC of Greater Boston. Also at the National, Chris Miele s Flirt took fourth place in 9-12 Puppy Bitches. By early November, Helen Sherman s Scoot earned her 5 th Senior Hunter leg. In Obedience she won a run-off to earn a third place in versatility, and went onto qualify in Open B. In Agility she qualified in Time 2 Beat, and ran really well in all her classes. The October issue of the Newsletter reported that Lisa Smith s young girl Secret, Goodtime s To Tell the Truth, earned two BOB s and a Group 3, all from the 12-18 month class, owner-handled. At that time Lisa emailed a photo of Secret. Unfortunately it never reached me, and is still floating around somewhere in cyber-space. I asked Lisa to re-send it for this issue, and here is Secret, looking beautiful as expected. Secret Goodtime s To Tell the Truth Kindle, who is officially known as Gaylans Hearts Afire, has some new agility titles: AX (Excellent Standard), AXJ (Excellent Jumpers with Weaves) and OF (Open Fast), reported by her owner-handler, Phoebe Teramoto. Kindle also earned a leg in Masters JWW. During the fall, she earned her WC title and a JH leg. After all their Agility placements, Kindle and Bette found time to visit Santa.

!! Our Stars cont d!! Phyllis Ward s Campbell received an award of merit at the National Specialty. Also at the National, her Bubbles made the final cut in American Bred bitches. Then, just a few weeks after winning an Award of Merit at the GRCA National, Campbell completed his Novice JWW Agility Title at the Springfield shows, handled by Mary Elizabeth Simpson. He got all three legs with placements and earned two Novice Standard legs as well. Congratulations to Phyllis and this very talented boy who is nearly 10 years young! Club Activities HVGRC Members Results From the HVGRC November Agility Trials Submitted by Chair, Chris Miele Friday November 7 Open Std 20 Open JWW 20 Excellent JWW 20 Preferred Novice JWW 20 Novice Std Time 2 Beat 1 st Place Jacaranda s Just In Case, Alyson Fuge 1 st Place Jacaranda s Just In Case, Alyson Fuge Q Parhelion s Ryan of the Highlands, Joe Chvastek 1 st Place GCH Award s Follow Your Bliss, Phyllis Ward 3 rd Place Matilda, Chris Miele 2 nd Place Cross Creek Royal Blend, Chris Miele Saturday November 8 20 Masters JWW 3 rd Place Gaylan s Hearts Afire, Phoebe Teramoto Novice FAST 3 rd Place Matilda, Chris Miele Sunday November 9 Open Std 20 2 nd Copper Lee Abbie Einstein, Mike Jones 20 Masters JWW 2 nd Gaylan s Silver Springs Ducks Gotta Fly CDX SH, Helen Sherman Q Gaylan s Hearts Afire, Phoebe Teramoto Time 2 Beat 20 4 th Cross Creek Royal Blend, Chris Miele Karen Fennikoh was the hostess with the most-ess for our October 15 meeting. Not only did she serve and coordinate the great food, and allow club members to examine her litter of seven-week-old puppies, she even had a flying monkey to lend a little Halloween cheer, a Grand Champion flying monkey, no less! GCH. Amberglo's High Velocity "Zoom" 5

Club Activities cont d Specialty Raffle Hi everyone, I hope you all had a wonderful holiday and it was everything you were wishing for. Now that they re over and we re into the winter doldrums, it s a good time to start thinking about the HVGRC 2015 Specialty Show in June. I m hoping to enlist the support of each member in making this year s raffle as successful as last year...maybe even better. We can do it!! A small contribution from every member will make it happen. I want to thank Chris Miele and Giselle Graham for already giving me some great items at the November meeting a beautiful signed golden retriever ornament, a gorgeous basket, leashes & collars, canine coasters, a canvas golden retriever tote bag, toys, clickers for training, food bowls and treat bags. Thank you! Thank you!! Thank you!!! Joe and I recently attended two fund raisers given by friends one for a pre-school and one for a cancer support group. Some of the raffle items were simply amazing. I asked my friends where they got the donations and they both said, friends asking friends. There are so many willing people who would donate to our raffle all you have to do is ask! Perhaps you got a gift over the holidays that you re not wild about and you are planning to return it. Consider our raffle there s probably someone who d love that gift you hate. And remember, the items don t have to be dog related. Big hits last year were the Keurig, the Seiko watches and the photo shoot. After the holidays retail sales are phenomenal. Be on the lookout for marked-down items that could be raffled separately or grouped together in a basket. Baskets based on a theme are a big draw - people love them. I have the baskets all you have to do is give me the items to fill them. Pick a theme, ask a few friends to supply an item each and we ve got a basket! Last year Joe and I thought of the theme Fun in the Sun. We got a straw basket and filled it with flip flops, sunglasses, a beach towel, a chair umbrella, a couple of summer books, a straw hat, and a water bottle. It was all about $30 from a discount store and it sold lots of raffle tickets!!!! More examples are sports related items, gifts for kids, jewelry, chain store gift cards, lessons of any kind (horseback riding, golf, dancing, etc.), books, wine, chocolate & cookies, candles, picture frames, household items and of course, any pet products. The possibilities are endless. Use your imagination. Think of all the people you know, groups you belong to, and places you frequent. Many of them will be happy to contribute something if you just ask!! Be sure to tell people that we are a non-profit organization who makes monetary contributions towards rescue, research and education. Much of the research has helped canines of all breeds and even humans as well. Thank you for reading. Wishing all of you a very Happy & Healthy New Year 2015!! Lorraine Cusimano Kira3@comcast.net 845 278 4062 P.S. Contact me if you need pick up. Also, I will be at the January 25th Health Clinic if you want to bring anything then. DEADLINES FOR NEXT ISSUE April 1st Deadline for News April 15th Mailing Date HOW TO SUBMIT NEWS All news must be submitted in legible, written form received by the editor before the deadline. (Earlier is better). Do not call at the last minute to say Will you please put in a notice about Write it yourself and send it to me. E-mail is fine: shandel@optonline.net. 6

Hudson Valley Golden Retriever Club OFFICERS PRESIDENT Robert Swanson PO Box 220, Sherman CT 06784 (H)860/354-9416 (C) 860/318-1132 overlookgoldens@gmail.com VICE PRESIDENT Christine Miele 238 Sarah Wells Trail, Campbell Hall, NY 10916 (H) 845/615-1565 (C) 834/649-2819 XCREEK@aol.com TREASURER OPEN POSITION RECORDING SECRETARY Carol Russo 9 Strawberry Hill Court, Montvale, NJ 07645 (H) 201/694-0058 signetm@optonline.net CORRESPONDING SECRETARY Bonnie Oddo PO Box 544, Lincoln Park NJ 07035 (H) 973/583-1021 bonoddo@gmail.com STANDING COMMITTEE CHAIRMEN AKC PUBLIC ED COORDINATOR Sandy Spitzer russan@frontiernet.net FIELD REP Position Open: Volunteer Needed GRCA DELEGATE Chris Miele xcreek@aol.com GRCA NEWS Robert Swanson overlookgoldens@gmail.com HISTORIANS Vincent Savarese & Karen Fennikoh (H) 845/361-5944 BOARD OF DIRECTORS CLASS OF 2016 Ann Marie Cioffi Caseyberry@aol.com Lynn Halpern Lynn1@att.net CLASS OF 2015 Helen Sherman HshermanK9Gold@aol.com Brian Visokay 176 Pudding St., Putnam Valley, NY 10579 (H)845/528-0516 (C) 845/528-5127 Brian@brvisokay.com NEWSLETTER EDITOR Susan Handelman 40 Birch Drive, Katonah NY 10536 (H) 914/232-1714 shandel@optonline.net LAYOUT/E-NEWS EDITOR Melissa Fairweather parhelion@hvc.rr.com MEMBERSHIP Lisa Weinberg WindrushGoldenRetrievers@gmail.com PROGRAM Position Open: Volunteer Needed PUPPY REFERRAL Binnie Weissleder Dogzrus1@comcast.net VOLUNTEER REQUIREMENTS Giselle Graham Jgraham54@sbcglobal.net For more information go to our website: hvgrc.org 7

BITS AND PIECES PUPPY REFERRALS When listing your puppies with Binnie Weissleder (802) 375-9527 or dogzrus1@comcast.net please observe the following: Call her within a week of the litter s due date to confirm that the litter actually has been whelped and to let her know how many puppies are available. Don t forget to let her know when all the puppies have been sold. Please also let Binnie know if you have any older dogs available. PLEASE NOTE! HVGRC Puppy Referral Guidelines, in effect since August 1, 1998, require that a signed release form for each litter be completed and returned to Binnie and Mike. If you need a copy of this form, call Binnie. Breeders Please Note: HVGRC subscribes to the same Code of Ethics as GRCA. Members are advised to keep informed of any changes now being considered. NEWSLETTER ADVERTISEMENTS Litter Listings or Items for Sale There is no charge for these kinds of advertisements. The following requirements still apply: In conformity with HVGRC Puppy Referral Guidelines, ads for puppies must be accompanied by copies of parents hip, elbow, eye and cardiac clearances. NOTICE re PUPPY ADVERTISEMENTS: The Hudson Valley Golden Retriever Club makes no warranties or guarantees as to quality, health or any other matters with respect to either the dogs listed herein or the puppies which have or may be produced as a result of the breedings listed herein. The HVGRC recommends that a fully executed, written purchase agreement setting forth all the terms of purchase be obtained before possession of a puppy is transferred to the purchaser(s). HVGRC WEBSITE Visit the HVGRC Website frequently for the latest information: hvgrc.org. Please note: Anyone wishing to post news or notice on the website, should send it to our President, Bob Swanson, who will then transmit it to our webmaster. PROSPECTIVE MEMBERS In order to qualify for membership in HVGRC you must first attend two club functions. For application form and further details, contact our Membership Chairperson, Lisa Weinberg, at (914) 248-8245 (H). Her personal e-mail is WindrushGoldenRetrievers@gmail.com or through the club membership@hvgrc.org. SPECIAL TROPHIES Attention: potential donors! Anyone who wishes to donate a Special Trophy for our Annual Specialty Show (in addition to the regular club trophies) needs to plan ahead. It is necessary to submit a descriptive letter to the Board of Directors for their approval by April of the previous year. For example, for our June 2016 Show, such a letter must reach the Board by April 2015. Prizes may be offered in honor of, in memory of etc. Be sure to include a full description of the trophy itself. If something inspires you, write to the Board. NEWSLETTER DISCLAIMER Following GRCA guidelines, the HVGRC Board has adopted the following policy for articles submitted by individuals: Signed or featured articles express the opinion of the author and do not necessarily reflect the views of HVGRC. The responsibility for factual accuracy and documentation belongs solely to the author of the article. From the Board of Directors: Attention all chairmen: It is a club policy that all published materials including but not limited to flyers, premium lists, and GR News columns be submitted to the Board of Directors for review prior to being released. New members please note: Please refer to the HVGRC web site for Guidelines and Requirements for Annual Awards. This material is no longer included in the new member packet. HVGRC E-MAIL Use this address whenever you need to communicate with the full membership: hvgrc@yahoogroups.com 8

ALL BREED SUPER HEALTH CLINIC HIPS ELBOWS EYES HEART THYROID MICROCHIP PRA ICHYTHYOSIS TICK DISEASE Sunday, January 25, 2015 8am 4 pm Norwalk Veterinary Hospital, 726 Connecticut Avenue (Rte 1), Norwalk, CT QUESTIONS jgraham54@sbcglobal.net 203-270-8406 XRAYS HIPS $240.00 Includes image CD, OFA fee. No Interpretation. Dr Charles Duffy $290.00 Dogs OVER 80 Lbs. Dr. Charles Duffy ELBOWS $225.00 Includes image CD, OFA Fee. No Interpretation. Dr Duffy $275.00 Dogs OVER 80 Lbs. Dr Duffy HIPS &ELBOWS $300.00 Includes image CD, OFA Fee.. Dr Duffy $350.00 Dogs more than 80lbs (note we cannot accept dogs more than 100 lbs for either hips or elbows) All X-rays will be submitted to OFA. You will not be informed of the results at the clinic. The correct OFA Forms MUST be included with your registration. CD s will be mailed to you after the clinic. NOTE: Dogs scheduled for hips and/or elbows cannot be given any food or water after 9PM the night before. Dogs are sedated with Xylazine. EYES $38.00 CERF, Dr Michael Brown (Board Certified) HEART $40.00 Auscultation. Doppler available $250 Dr. Jean- Paul Petrie (board certified) THYROID $90.00 MSU OFA Panel (includes OFA fee) **** Bring a stamped, self-addressed envelope to the clinic to receive MSU results MICROCHIP $40.00 SNAPP 4DX $50 lyme, anaplasma, erlichiosis, heartworm. Immediate results OPTIGEN DNA Tests $20.00 Blood Draw/Sample Submission fee per dog payable to HVGRC Optigen Test Request Forms must be completed after Janurary 5th ONLINE at Optigen using clinic code HVGRCx4 for an additional 5% off, making the total discount 25%. Bring Completed Paid in Full Form and confirmation number to the clinic. Test Full Price 20% from clinic 20% multi test discount Prcd-PRA $195 $156 $124.80 $117 GR-PRA1 $100 $80 $64 $60 GR-PRA2 $100 $80 $64 $60 Ichythyosis $120 $96 $96 $90 TOTAL $515 $412 $348.80 $327 5% online payment 9

ALL BREED SUPER HEALTH CLINIC, JANUARY 25, 2015 PRE REGISTRATION ONLY Deadline January 15, 2015 Questions: email: jgraham54@sbcglobal.net Phone: 203-270-8406 MAIL Completed Form with CHECK PAYABLE TO HVGRC TO: Giselle Graham, 32 Old Farm Hill Rd, Newtown, CT 06470 Print Your Name: Phone: Cell: Address: City: State: Zip: email: TIME SLOT PREFERRED, 8:00-12:00 Noon 12:00-4:00 Services Put an X in each box under each name. Call Name Call Name Call Name Call Name Call Name Unit Cost XRAYS: pricing includes OFA Fee and image CD. No interpretation offered. Completed OFA Form MUST accompany this application = Amt. HIP XRAY Only X $240 HIP XRAY dogs over 80 lbs X $290 ELBOW XRAY Only X $275 ELBOW XRAY dogs over 80 lbs X 300 HIPS & ELBOWS Combined HIPS & ELBOWS Combined dogs over 80 lbs ACVO EYE EXAM on OFA Form CARDIAC AUSCULTATION CARDIAC ECHOCARDIOGRAM MICROCHIP SNAP 4 - Heartworm/Lyme/Ehrlichia/ Anaplasmosis MSU THYROID PANEL (includes OFA fee ) (Must bring stamped self-addressed envelope to receive detailed panel results. OPTIGEN DNA TESTS Blood Draw (per dog regardless of tests ordered) X $300 X$350 X $38 X $40 X 250 X $40 X $50 X $ 90 X $20 TOTAL 10

The Genetics of Subvalvular Aortic Stenosis (SAS) in Golden Retrievers Presented by Dr Josh Stern, DVM, PhD, ACVIM, UC Davis at the 2014 Golden Retriever National Specialty Personal notes taken by Dr Gayle Watkins Table of Contents What is of Subvalvular Aortic Stenosis (SAS)?... 1 How is SAS Diagnosed?... 2 What Are the Flow Rate Cut Off Values for GRs?... 2 Are There Treatments For SAS?... 3 SAS Research in Goldens... 3 Genetics of Golden Retriever SAS... 4 Age for Reliable Screening... 5 Where Are We On a GR DNATest?... 5 If You Have A Dog with SAS... 5 When the DNA Test Becomes Available... 6 Update since this talk was given at the National by Gayle Watkins... 6 More Info on Subvalvular Aortic Stenosis... 7 What is of Subvalvular Aortic Stenosis (SAS)? SAS is the 2 nd most common cardiac disease in dogs. It is characterized by aortic subvalvular blood flow restriction Mild SAS does not affect lifespan or activity Severe SAS lifespan averages 19 months SAS disease characteristics Left ventricular concentric hypertrophy Subvalvular lesion can look subtle but causes heart to overwork, causing ischemic infarction and arrhythmia Page 1 Copyright 2014 Gayle L Watkins 11

The Genetics of SAS in Golden Retrievers, 2014 Golden Retriever National Specialty Health Seminar Post stenotic dilation Aortic insufficiency How is SAS Diagnosed? Gold standard diagnosis (dx) post mortem necropsy of subvalvular aorta Ante mortem (before death) o old way was angiogram, now echocardiograms are used o But dog must be calm during echo o Extreme duress will not result in a valid echo. It can change a normal to an equivocal. o If your dog has an equivocal rating at a clinic, go to a cardiologist to confirm the dx. Auscultation alone isn t good enough to dx SAS o Almost all breeding age golden retrievers (GRs) will develop soft murmur with excitement o Doppler echo is the only way to determine if a dog is truly abnormal You cannot overestimate aortic velocity since higher numbers are more accurate than lower o An abnormal result in a calm dog should be taken seriously o Clear by auscultation (http://en.wikipedia.org/wiki/auscultation) alone doesn t mean much to Dr Stern. (Note from Gayle: But then Dr Stern went on to say that we do not need to echo all breeding stock. How they do we clear them?) o For more info on echocardiograms in dogs, see http://www.offa.org/cardiac_echo.html. What Are the Flow Rate Cut Off Values for GRs? Flow rates or velocities are measured during a Doppler echo in meters per second or m/sec. The categorization of these rates has changed over the past few years. There is still not consensus among cardiologists. The rates in the right hand column are what Dr Stern thinks are appropriate. Page 2 Copyright 2014 Gayle L Watkins 12

The Genetics of SAS in Golden Retrievers, 2014 Golden Retriever National Specialty Health Seminar ARCH Past Today Normal 1.9 m/sec 2.0 m/sec Abnormal 2.4 m/sec 2.4 m/sec Equivocal 2.0 to 2.4 m/sec 2.1 to 2.4 m/sec Are There Treatments For SAS? With treatments, severely affected dogs can live to 4.5 years But no interventional therapy changes clinical outcome Medical management may delay onset of severe disease Clinical outcomes o Syncope (transient loss of consciousness) o Sudden death o Congestive heart failure SAS Research in Goldens In 1976, research determined Newfoundlands SAS was autosomal dominant with incomplete penetrance. It is also familial in GRs and Rottweilers, thus affected dogs produce more affected dogs than unaffected dogs do. SAS is found in low frequencies in breeds other than Newfs, GRs and Rotties. SAS is rare in people so we can t use human research. 2012 Stern Study http://www.ncbi.nlm.nih.gov/pubmed/22417094 (see abstract at end of notes) 73 GRs with 3 generation pedigrees o All had full ACVIM Doppler o Affected 2.50 m/sec o Normal 1.8 m/sec o Equivocal between those values Page 3 Copyright 2014 Gayle L Watkins 13

The Genetics of SAS in Golden Retrievers, 2014 Golden Retriever National Specialty Health Seminar Of the 32 Affected GRs (10 M, 22 F) o 37.5% had left ventricular hypertrophy (LVH; thickened ventricle) o 67% subvalvular ridge o 67% leaking aortic valve For every 1 m/sec above 2.5 m/sec o 2.95 x more likely to have subvalvular ridge o 4.65 x more likely to have LVH o 2.54x more likely to have leaking aortic valve So if you have a dog with an equivocal, look for these other issues (ridge, LVH, leaking valve) to try to determine if the dog is actually normal or affected Genetics of Golden Retriever SAS In goldens, the inheritance pattern is not simple. It is most likely: Polygenic Autosomal dominant with incomplete penetrance When we eventually get a genetic test if it supports an autosomal dominant with incomplete penetrance mode of inheritance, do NOT test and remove animals from gene pool!!! If the gene is rampant in the breed, breeders must strategically: remove dogs breed Do NOT breed SAS affected dogs But do not throw out the dogs with the gene that do not express the disease! For them, use outcrossed breedings. He is not optimistic that Estimated Breeding Values (EBVs) will be useful because we will need velocities for all dogs in the pedigree Page 4 Copyright 2014 Gayle L Watkins 14

The Genetics of SAS in Golden Retrievers, 2014 Golden Retriever National Specialty Health Seminar Age for Reliable Screening Can screen with echo in young dogs Puppy murmurs should be gone by 4 months If there is a loud murmur after 4 month rabies shot, consider SAS New echo test uses different calculations (see values above) The exercise test for GRs is USELESS Where Are We On a GR DNATest? There is NO DNA test appropriate for golden retrievers though researchers are working on one. They have identified the gene mutation in Newfies and are manipulating the gene (http://news.ucdavis.edu/search/news_detail.lasso?id=10979) BUT DO NOT USE THE NEWFIE TEST FOR GRs!!! The Newfoundland gene is NOT associated with SAS in GRs. In goldens, they are looking at Chromosome 21. If You Have A Dog with SAS You can help by submitting a blood sample: Whole blood in purple topped tube 3 generation pedigree Report from a cardiologist s echo examination If severely affected, consider a necropsy donation to research Veterinary Genetics Lab, NC State s Veterinary Health Complex (http://www.ncstatevets.org/ongoing research/) or UC Davis Veterinary Genetics Lab (www.vgl.ucdavis.edu) Page 5 Copyright 2014 Gayle L Watkins 15

The Genetics of SAS in Golden Retrievers, 2014 Golden Retriever National Specialty Health Seminar When the DNA Test Becomes Available Consider using the researchers lab for testing so: The researched can gain access to samples Many university labs donate back to lab Commercial labs do not share samples so there is no research benefit Update since this talk was given at the National by Gayle Watkins Since Dr Stern s talk at the 2014 Golden Retriever National Specialty, at least one DNA testing lab, Paw Print Genetics has discontinued offering the PICALM test. This decision was based on new findings from Michaela Drogemuller which dispute the results of Dr Stern s research. Dr Stern is aware and supportive of this decision, based on his comments on Facebook. The PICALM gene is not suspected to be the cause of golden retriever SAS so this new evidence does not affect golden retriever breeders and owners. However, the debate between these two teams is a good reminder to us to scrutinize DNA tests and the research they are based on before they are used widely to remove dogs from the gene pool. Drögemüller M, Jagannathan V et al. 2014. A single codon insertion in the PICALM gene is not associated with subvalvular aortic stenosis in Newfoundland dogs. Human Genetics. Nov 13. [Epub ahead of print] Reist Marti SB, Dolf G et al. 2012 Genetic evidence of subaortic stenosis in the Newfoundland dog. Veterinary Record. Jun 9;170(23):597. http://www.ncbi.nlm.nih.gov/pubmed/?term=22645160 Page 6 Copyright 2014 Gayle L Watkins 16

The Genetics of SAS in Golden Retrievers, 2014 Golden Retriever National Specialty Health Seminar More Info on Subvalvular Aortic Stenosis Golden Retriever Club of America, The Big Four: Hips, Elbows, Eyes, Hearts. http://www.grca.org/health/bigfour.html Today s Breeder, Getting Serious about SAS. http://www.grca.org/pdf/health/heart SAS.pdf AKC Canine Health Foundation, Aortic Stenosis http://www.akcchf.org/caninehealth/your dogs health/disease information/aortic stenosis.html Orthopedic Foundation for Animals, The Echocardiographic Exam http://www.offa.org/cardiac_echo.html. Stern, JA, Meurs, KM et al. 2012. Familial subvalvular aortic stenosis in golden retrievers: inheritance and echocardiographic findings. Journal of Small Animal Practice. Apr 53(4):213 6. Dr Josh Stern jstern@ucdavis.edu http://www.ncbi.nlm.nih.gov/pubmed/22417094 Abstract OBJECTIVES: To describe the echocardiographic findings and pedigree analysis of golden retrievers with subvalvular aortic stenosis. METHODS: Seventy three golden retrievers were evaluated by auscultation and echocardiography. A subcostal continuous wave Doppler aortic velocity >2.5 m/s and presence of a left basilar systolic ejection murmur were required for diagnosis of subvalvular aortic stenosis. Three echocardiographic characteristics were recorded: evidence of aortic insufficiency, subvalvular ridge or left ventricular hypertrophy. A disease status score was calculated by totalling the number of echocardiographic characteristics per subject. RESULTS: Thirty two of 73 dogs were affected and their aortic velocities were as follows: range 2.5 to 6.8 m/s, median 3.4 m/s and standard deviation 1.2 m/s. Echocardiographic characteristics of 32 affected dogs were distributed Page 7 Copyright 2014 Gayle L Watkins 17

The Genetics of SAS in Golden Retrievers, 2014 Golden Retriever National Specialty Health Seminar as follows: left ventricular hypertrophy 12 of 32, aortic insufficiency 20 of 32 and subvalvular ridge 20 of 32. Disease status score ranged from 0 to 3 with a median of 2. There was a statistically significant correlation between aortic velocity and disease status score (r=0.644, P<0.0001). Subvalvular aortic stenosis was observed in multiple generations of several families and appears familial. CLINICAL SIGNIFICANCE: Subvalvular aortic stenosis in the golden retriever is familial. Severity of stenosis correlates well with cumulative presence of echocardiographic characteristics (left ventricular hypertrophy, subvalvular ridge and aortic insufficiency). Page 8 Copyright 2014 Gayle L Watkins 18