Key words: Campylobacter, diarrhea, MIC, drug resistance, erythromycin

Key words: Campylobacter, diarrhea, MIC, drug resistance, erythromycin

Table 1 Detection rate of Campylobacter from stool samples taken from sporadic diarrheic patients

Table 2 Detection rates of Campylobacter in 16 to 30 year old patients Table 3 Distribution of MICs for 127 C. jejuni and 8 C. coli strains isolated in human stools from 2001 to 2003 in Japan GM. gentamicin ; EM, erythromycin ; CPFX, ciprofloxacin ; TC, tetracycline

Table 4 Resistant rates of four antimicrobaial agents to C. jejuni and C. coli Table 5 Resistant rates of two antimicrobial agents to C. jejuni isolates between the periods of January 2001-June 2002 and July 2002-December 2003

Table 6 Erythromycin susceptibility to C. jejuni and C. coli using the agar dilution, Etest, and disk diffusion tests

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Antimicrobial Susceptibility of Campylobacter jejuni and Campylobacter coli Isolated from Human Diarrheic Samples Sadao TAKAYAMA1), Sachiko SATAKE2) & Kanako ISHIHARA3) Isesaki Municipal Hospital 1) School of Health Sciences, Gunma 2) University National Veterinary Assay Laboratory, Ministry of Agriculture, 3) Forestry and Fisheries One hundred forty-seven Campylobacter were isolated using 3,204 samples taken from sporadic diarrheic patients from January 2001 to December 2003. The detection rate of Campylobacter in 16 to 30 year old patients (12.9%, 83/641) was significantly higher than that in patients less than16 years of age, 5%, (29/1,155)(p< 0.001) and more than 30 years of age, 2.5% (35/1,408) (p < 0.001), respectively. The highest detection rate was obtained from the stool of males in the 16 to 30 year old range during the months from May to August, 26% (32/123). If the minimal inhibitory concentration (MIC) breakpoint for resistance of gentamicin (GM), erythromycin (EM), ciprofloxacin (CPFX), tetracycline (TC) were defined would be 0.0%, 0.0%, 22.0%, 42.8% in C. jejuni, 0.0%, 62.5%, 62.5%, 87.5% in C. coli, respectively. All the Campylobacter isolates were susceptible to GM. Three of the 8 C. coli isolates were multi-resistant in EM, CPFX, and TC. Five highly EM resistant strains with an MIC zone around the EM disk ; 7 susceptible strains with an MIC of less than 24mm to 36mm and revealed a good correlation with the Etest method and the agar dilution method. Between the two time periods of January 2001-June 2002 and July 2002-December 2003, the resistant rate of CPFX in C. jejuni decreased from 27.5% to 15.5%, however, that was not significant decrease (p= 0.133).