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UNIVERSITI PUTRA MALAYSIA EFFECTS OF PROGESTERONE INTRAVAGINAL DEVICES ON ARTIFICIAL INSEMINATION, OESTROUS SYNCHRONIZATION, OVULATION AND PREGNANCY RATE IN KEDAH-KELANTAN CATTLE KHOR CHUN SIAH FPV 2002 8

EFFECTS OF PROGESTERONE INTRA VAGINAL DEVICES ON ARTIFICIAL INSEMINATION, OESTROUS SYNCHRONIZATION, OVULATION AND PREGNANCY RATE IN KEDAH-KELANTAN CATTLE By KHOR CBUN SlAB Thesis Submitted to the School of Graduate Studies, Universiti Putra Malaysia, in Fulfilment of the Requirements for the Degree of Master of Science November 2002

Abstract of thesis presented to the Senate ofuniversiti Putra Malaysia in fulfilment of the requirement for the degree of Master of Science. EFFECTS OF PROGESTERONE INTRA VAGINAL DEVICES ON OESTROUS SYNCHRONIZATION, OVULATION AND PREGNANCY RATE IN KEDAH-KELANTAN CATTLE By KHOR CHUN SlAB November 2002 Chairman: Assoc. Prof. Dr. Abd Wahid Baron, D.V.M., Ph.D. Faculty: Veterinary Medicine The first experiment was conducted to evaluate the effect of various progestagen treatment methods in term of oestrous synchronization, ovulation and pregnancy rate using controlled internal drug-release (CIDR) device or progesterone-releasing intravaginal device (PRID) with prostaglandin F2ct (pgf2ct) injection on the day of CIDRIPRID removal. Twenty-four non-pregnant Kedah- Kelantan (KK) cows were randomly assigned to two groups: CIDR (n=12); PRID (n=12). Both group were given 12-day synchronization treatment and received an intramuscular injection of 25 mg PGF2ct on the day of device removal. The ovaries of all cows were examined by ultrasonography, using a 5 MHz probe, from one day after CIDRIPRID removal until ovulation. Blood samples were collected for determination of progesterone. The proportion of cows observed in oestrus was significantly higher in the CIDR group than PRID group (91.7% vs 58.3%, P<O.Ol). Pregnancy rate was also significantly higher in CIDR group than PRID group 11

(27.3% vs 1 4.2%, P<0.05). Results from experiment indicated that CIDR is more efficient than PRID. The second experiment was to evaluate the effect of the length of progestagen treatment (12 day vs 7 day) on oestrous synchronization, ovulation and pregnancy rate using controlled internal drug-release device (CIDR) with PGF2a injection at the end of CIDR treatment. Sixteen adult non-pregnant KK cows were divided into two groups: long-term treated group (n=8); Short-term treated group (n=8). day of CIDR removal. All the cows were undergone transrectal ultrasonography, and blood samples were taken for hormone assay. Until 120 h after CIDR removal, the percentage of cows came into oestrus was significantly higher in long-termtreated group than short-term treated group (87.5% The pregnancy rate was significantly higher in the short-term treated group than in long-term-treated group (40.0% vs 25.0%, follicle in long-term treated group attained a larger maximum diameter than in short-term treated group (P<0.05). The mean time of ovulation after device removal was 108 h in the short-term treated group and 88 ± 5.06 h in the long-tenn treated group; the length of time required for ovulation between two treatment were significantly different (P<0.05). The result showed that the 7-d CIDR+ PGF2a oestrous synchronization protocol rendered a better fertility in Kedah-Kelantan cows. 11l

Abstrak tesis yang dikemukakan kepada Senat Universiti Putra Malaysia sebagai memenuhi keperluan untuk ijazah Master Sains. KAJIAN KESAN ALAT INTRA VAGINA (CIDR ATAU PRID) KEATAS PERMANIAN BERADAS, PENYAMAAN GALAK, OVULASI DAN KADAR KEBUNTINGAN PADA LEMBU JENIS KEDAB-KELANTAN Oleh KHOR CHUN SlAB November 2002 Pengerusi: Fakulti: Prof Madya Dr. Abd Wahid Baron, D.V.M., PH.D. Perubatan Veterinar Kajian pertama dijalankan untuk mengenalpasti kesan kaedah perlakuan progestagen dengan menggunakan dua jenis alat intravagina iaitu alat CIDR (Controlled internal drug-release device) dan PRID (Progesterone-releasing intravaginal device) bersama suntikan prostaglandin F2u (PGF2a) pada hari CIDRIPRID dikeluarkan ke atas penyamaan galak, ovulasi dan kadar kebuntingan. Dua puluh empat ekor lembu jenis Kedah-Kelantan yang dikenalpasti tidak bunting dibahagikan secara rambang kepada dua kumpulan: CIDR(n= 12); PRID (n=12). Kedua-dua kumpulan diberi perlakuan penyamaan galak 12 hari dan satu suntikan 25mg PGF2a di bahagian otot pada hari alat tersebut dikeluarkan. Ovari semua lembu diperiksa ultrasonografi dengan menggunakan 5 MHz proba dari satu hari selepas CIDRIPRID dikeluarkan sampai berlakunya ovulasi. Sampel darah diambil untuk penentuan paras progesteron. Kumpulan CIDR menunjukkan kadar kedatangan estrus amat bererti yang lebih tinggi berbanding dengan kumpulan PRID (91.7% vs 58.3%, P<O.Ol). Kadar kebuntingan amat bererti yang lebih tinggi IV

juga telah diperolehi pada kumpulan CIDR berbanding kumpulan PRill (27.3% vs 14.2%, P<0.05). Keputusan ujikaji menunjukkan bahawa alat CIDR adalah lebih efisyen berbanding dengan PRill. Kajian kedua dijalankan untuk menentukan kesan perlakuan jangkamasa alat intravagina CIDR (12 hari vs 7 hari) ke atas penyamaan galak, ovulasi dan kadar kebuntingan. Enam belas ekor lembu jenis Kedah-Kelantan yang dikenalpasti tidak bunting dibahagikan secara rambang kepada dua kumpulan: kumpulan 12 hari (n=8) dan kumpulan 7 hari n=8). Kedua-dua kumpulan di beri satu suntikan 25mg PGF2a. di bahagian otot pada hari alat CIDR dikeluarkan. Semua lembu telah diperiksa dengan alat ultrasound melalui rectum dan sample darah diambil untuk ujian hormon. Sehingga 120 jam selepas CIDR dikeluarkan, kumpulan 12 hari menunjukkan kadar kedatangan estrus amat bererti yang lebih tinggi berbanding dengan kumpulan diperolehi pada kumpulan 7 hari (87.5% vs 62.5%, P<0.05). Kadar kebuntingan amat bererti yang lebih tiggi telah diperolehi pada kumpulan 7 hari berbanding dengan 12 hari (40.0% vs 25.0%, P<0.05). Follikel ovulasi di kumpulan 12 hari mencapai diameter maksimum yang lebih besar berbanding dengan kumpulan 7 hari (P<0.05). Kadar masa ovulasi selepas CIDR dikeluarkan adalah 108 jam bagi kumpulan 7 hari dan 88 ± 5.06 jam bagi kumpulan 12 hari (P<0.05). Jangkamasa ovulasi bagi kedua-dua kumpulan menunjukkan perbezaan yang bererti (P<0.05). Keputusan ujikaji menunjukkan bahawa perlakuan 7 hari CIDR + PGF2a. adalah kaedah penyamaan galak yang lebih sesuai untuk mendapat kesuburan yang lebih tinggi pada lembu jenis Kedah-Kelantan. v

ACKNOWLEDGEMENTS I would like to express my heartfelt gratitude and deepest appreciation to Assoc. Prof Dr. Abd. Wahid Raron, Chairman of the Supervisory Committee, for his invaluable guidance, patience and encouragement throughout the course of my study. I am very grateful to Dr. Abas Mazni Othman and Dr. Rosnina Rj. Yusoff, members of the Supervisory Committee, for their suggestions on technical problems, invaluable guidance and advice in the preparation of this thesis. I am indebted to Mr. Mohd. Padzil A. Rahman of MARDI, Mr. Yap Keng Chee and Mr. Abu Bakar Dahri of UPM for their technical assistance: Mr. Mohd. Padzil for performing the artificial insemination, Mr. Yap Keng Chee for his guidance in hormone analysis and Mr. Abu Bakar for assisting in detection of oestrus. My deepest appreciation also to fellow graduate students, Dr. Myint Thein and Mrs. Riasari Gail Sianturi, for their invaluable help, advices and suggestions during the thesis preparation. My profound gratitude to my parents, Mr. Khor Seow Kee and Mrs.Teoh Goik Riah for their love, understanding and support during my period of study. I wish to extend my thanks to my brothers and sister for their encourangement and moral support. VI

Finally, I wish to thank my fiancee, Ms. Tiong Siew Ing for her support in my career. VB

I certify that an Examination Committee met on 5th November 2002 to conduct the final examination of Khor Chun Siah on his Master of Science thesis entitled "Effects of Progesterone Intravaginal Devices on Artificial Insemination, Oestrous Synchronization, Ovulation and Pregnancy Rate in Kedal:. -Kelantan Cattle" in accordance with Universiti Pertanian Malaysia (Higher Degree) Act 1980 and Universiti Pertanian Malaysia (Higher Degree) Regulation 1981. The committee recommends that the candidate be awarded the relevant degree. Members of Examination Committee are as follows: Nadzri Salim, D.V.M., M.V.S., M.P.V.M. Faculty of Veterinary Medicine Universiti Putra Malaysia (Chairman) Abd. Wahid Haron, Ph.D. Associate Professor Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine Universiti Putra Malaysia (Member) Abas Mazni Othman, Ph.D. Malaysia Agricultural Reserch and Development Institute (Member) Rosnina Yusoff, Ph.D. Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine Universiti Putra Malaysia (Member) AMSHER MOHAMAD RAMADILI, Ph.D. ProfessorfDeputy Dean School of Graduate School Universiti Putra Malaysia Date: 2 0 D C 2002 Vlll

This thesis submitted to the Senate of Universiti Putra Malaysia has been accepted as fulfillment of the requirements for the degree of Masters of Science. The member of the Supervisory Committee are as follows: Nadzri B. Salim, D.V.M., M.V.S., M.P.V.M. Faculty of Veterinary Medicine Universiti Putra Malaysia (Chairman) Abd. Wahid Haron, Ph.D. Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine Universiti Putra Malaysia (Member) Abas Mazni Othman, Ph.D. Malaysia Agricultural Reserch and Development Institute (Member) Rosnina Yusoff, Ph.D. Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine Universiti Putra Malaysia (Member) AINI IDERIS, Ph.D. ProfessorlDean, School of Graduate Studies, Universiti Putra Malaysia Date: 13 FEB 200J IX

DECLARATION I hereby declare that the thesis is based on my original work except for quotations and citations which have been duly acknowledged. I also declare that it has not been previously or concurrently submitted for any other degree at UPM or other institutions. Khor Chun Siah Date: ''l/i;;)' / oo;:;, x

TABLE OF CONTENTS Page ABSTRACT........................................................................... ABSTRAK......... ACKNOWLEDGEMENTS...... APPROVAL SHEETS... DECLARATION...,... LIST OF TABLES....................,....................................... LIST OF FIGlJRES.................................................................. LIST OF PLATES................................................... LIST OF ABBREVIATIONS......................................................... II IV VI Vlll x XU1 XlV xv XVI CHAPTER I GENERAL INTRODUCTION.......................................... I II LITERA TlJRE REVIEW......,... 4 2.1 Kedah-Kelantan cattle....,.............................. 4 2.1.1 Introduction..................... 4 2.1.2 Origin and Description of Breed..... 5 2.2 The Oestrous Cycle of Cows....................................... 6 2.2.1 Stages of the Cow's Oestrous Cycle...... '"... 6 2.2.2 Hormonal Control of the Oestrous Cycle...,... 9 2.3 Oestrous Synchronization............................................. 12 2.3.1 Synchronization by Progesterone and Progestagen....... 15 2.3.2 Synchronization by Prostaglandins and their Analogues.........................,. 18 2.3.3 Combined Treatment in Oestrus Control............ 20 2.4 Artificial Insemination...,... 22 2.5 Pregnancy Diagnosis in Cow.................. 23 III COMPARISON OF EFFECTIVENESS BETWEEN CIDR AND PRID ON SYNCHRONIZATION OF ESTRUS AND ASSOCIATED ENDOCRINE CHANGES IN KEDAH-KELANTAN CATTLE... 26 3.1 Introduction......................................................... 26 3.2 Materials and Methods....................................... 27 3.2.1 Animals......................................................... 27 3.2.2 Experimental Protocol........,..................... 28 3.3 Results......... 34 3.3.1 Oestrous Responses.......................................... 34 3.3.2 Follicular Growth and Ovulation..................... 36 3.3.3 Progesterone Concentration.............................. 38 3.4 Discussion... '"...................................................... 42 Xl

IV EFFECT OF CIDR TREATMENT PERIOD ON REPRODUCTIVE PERFORMANCE OF KEDAH-KELANTAN CATILE......... 46 4.1 Introduction...,... 46 4.2 Materials and Methods............'"..,......... 48 4.2.1 Animals... '"........................... 48 4.2.2 Experimental Protocol......,...,... 48 4.3 Results... '"............ '"...... 50 4.3.1 Oestrous Responses and Ovulation......, 50 4.3.2 Progesterone Concentration.............................. 56 4.4 Discussions...................................................... 58 V GENERAL DISCUSSION..................... 62 VI SUMMARY AND CONCLUSION.................................... 65 BIBLIOGRAPHY............................................................... 69 APPENDICES...... '".,... '"....,............................... 80 A Protocol on Progesterone Radioimmunoassay...... 82 VITAE.................................... 83 xu

LIST OF TABLES Table Page 1 Cows are randomly divided into CIDR-treated group and PRIDtreated treatment...,...... 30 2 Oestrus synchronization protocol for Kedah-Kelantan cattle treated with CIDR and PRID for 12 days, and intramuscular administration of 25mg PGF2a. after device removal... '"...... 31 3 Reproductive performance using different synchronization treatments in Kedah-Kelantan cows..................................... 34 4 Ovulatory responses in Kedah-Kelantan treated with CIDR and PRID................... 37 5 The effect of CIDR treatment duration on synchronized oestrus and pregnancy rate in Kedah-Kelantan cows... '" 51 6 Effect of CIDR treatment duration on the intensity of oestrus in Kedah-Kelantan cows... '"................................................ 52 7 Time of ovulation and mean maximum size of dominant follicle in Kedah-Kelantan cows after 7-d and 12-d treatment... '"......... 54 Xlll

LIST OF FIGURES Figure Page 1 Distribution of the onset of standing oestrus over time following treatment with CIDR and PRID..................... 36 2 Follicular growth in K-K cows after intravaginal device removal..................................................................... 38 3 Mean plasma progesterone concentrations in K-K cows treated with CIDR or PRID treatment......... '".,......................... 38 4 Plasma progesterone profiles of PRID treated cows without oestrus response... '"...,... " '"... '". 40 5 Plasma progesterone profiles of PRID treated cows without oestrus response during observation period... '"................................ 40 6 Plasma progesterone profile of CIDR treated cow (ID=II).................................................... '"............... 41 7 Plasma progesterone profiles of CIDR treated cows that showed oestrus response with no immediate occurrence of ovulation...... '"...... '"... 41 8 Plasma progesterone profile ofcidr treated cow (ID=22C)...... 42 9 Distribution of the onset of oestrus over time following treatment with 7-d CIDR and 12-d CIDR protocol.....,................ 52 10 Mean plasma progesterone in K-K cows treated with 7-d CIDR cow treatment and 12 -d CIDR treatment...,............. 5 6 11 Plasma progesterone profiles in 7-d CIDR treated cows that did not show any oestrus response... '".................................... 57 12 Plasma progesterone profile in 12-d CIDR treated cow that did not show any oestrus response............................................. 5 8 XIV

LIST OF PLATES Plate Page 1 An adult female Kedah;'Kelantan cow......,........................ 4 2 Controlled internal drug release (CIDR) device and its applicator... 29 3 Progesterone releasing intravaginal device (PRID) and its applicator...........,............ '"...... 30 4 Ultrasonography examination in a cow.............................. 32 5 Ultrasonographic image of preovulatory follicle ( early stage)....,.... 54 6 Ultrasonographic image of preovulatory follicle (later stage)...... 55 7 Occurrence of ovulation indicated by the absence of previous follicle...... '"..,... '"......... 55 xv

LIST OF ABBREVIATIONS AI CIDR CL CV Artificial insemination Controlled Internal Drug Release Device Corpus Luteum Coefficients of Variation Oestradiol FSH GnRH IU KK LH MARDI mg ng Follicle Stimulating Honnone Gonadotropin Releasing Honnone International Unit (s) Kedah-Kelantan Luteinizing Honnone Malaysian Agricultural Research and Development Institute milligram nanogram Progesterone PGF20: PRID QC Prostaglandin F 20: Progesterone-releasing Intravaginal Device Quality Control Regression SEM Standard Error of the Mean XVI

CHAPTERl GENERAL INTRODUCTION Kedah-Kelantan (KK) cattle is the predominant breed of cattle in Malaysia and represents the majority of Zebu cattle (Bos indicus) in Malaysia. To date, there is a paucity of information on the reproductive performance of this breed. Bos indicus cattle are tolerance to heat stress, highly resistance to ticks, of good fertility and able to thrive on a low plane of nutrition (Frisch and Vercoe, 198 4). However, they usually have lower potential for meat and lower fertility than Bos tauros (Dobson and Kamonpatana, 1986; Frisch et al., 1987; Lamothe Zavaleta et al., 1991 ). To overcome the small size of KK breed, crossbreeding programmes with exotic bulls were initiated by Malaysian Agricultural Research and Development Institute (MARDI) and other institutions in the country, in an effort to upgrade this breed. Artificial insemination (AI) could play an important role in the crossbreeding programme with breeds like Charolais, Limousin and Simental (Dahl an et ai., 198 5). AI is the most important and valuable single technique devised for the genetic improvement of animals (Odde, 1990), because it enables the widespread use of outstanding sires with valuable genetic potential to any livestock operation. Besides that, it facilitates progeny testing under a wide range of environmental and management conditions, thereby further improving accuracy

2 of selection leading to improved performance of the national herds. It also reduces risk of spreading sexually transmitted diseases like brucellosis, vibriosis (Ax et a1., 2000). Generally, the success of an AI breeding programme depends on adequate facility, good herd management, well-trained personnel and accuracy of oestrus detection (Dahlan et ai, 1985; Larson et ai, 1995). To minimize labour and management requirements in modem beef cattle breeding system, the oestrous cycle must be synchronized so that a large number of treated females come into oestrus at the preset time (Schmitt et ai., 1994; Van Cleeff et al., 1996; Pursley et al., 1997; Ryan et ai., 1999). There are two basic methods of synchronization of oestrous cycle in farm animals. The first method involves long-term administration of a progestagen so that the corpus luteum (CL) regresses naturally during the period when progestagen was administered. With this approach, the exogenous progestagen continues to exert a negative feedback on the luteinizing hormone (LH) secretion after regression of the CL. On progestagen withdrawal, follicular growth, oestrus and ovulation occur within 2 to 8 days. The second method induces the premature regression of a cyclic CL. The two primary luteolytic agents used are prostaglandin F2a (pgf2a) and oestrogen. A combination of both progestagen and luteolytic agent can also be use to give better oestrous synchronization in cow. In this approach, luteolytic agent is used to regress the CL and progestagen in the form of intravaginal devices are

3 used to mimic the action of progesterone and prevent oestrus until its withdrawal. A fixed time luteolytic agent injection and removal of the progestagen will stimulate oestrus to occur within a specific period of time. The objectives of this study were: 1. to determine the efficacy of two oestrous synchronization methods (CIDR versus PRID) in KK cows. 2. to document the response of KK cows to the two oestrous synchronization treatments. 3. to evaluate the effect of the length of CIDR treatment on follicular dynamics, oestrous synchronization and pregnancy rate in KK cows. 4. to determine the pregnancy rate after oestrous synchronization and AI.

CHAPTER 2 4 LITERATURE REVIEW 2.1 Kedah-Kelantan Cattle 2.1.1 Introduction Kedah-Kelantan (Plate 1) is the most common breed of beef cattle found in Malaysia. They are widely found in the states bordering Thailand. Thus, they were once called Kedah-Siam, Kedah-Thai and Thai-Kelantan cattle (Devendra, 1975). Cameons (1981) has classified these cattle as indigenous Zebu. They were once used as draught animals but now served as an important source of domestic beef in Malaysia. With improved management and nutrition, the live weight gain of KK had increased, the weight of adult KK averaged 240 kg and 180 kg, for male and female, respectively (Devendra et al., 1973). -.. f Plate 1. An adult female Kedah-Kelantan cow

2.1.2 Origin and Description of Breed 5 The origin of this breed is uncertain, but it is possible that they come from Thailan<L especially from the southern part where similar cattle are found. This fact is further reflected in they being concentrated in the states of Kedah, Kelantan and Trengganu; thus, the derivation of its name from the first two states. Initially however, it appears that this breed owes its ancestry to the humped Chinese yellow cattle of South Chinese Zebu cattle (Epstein, 1969). The migration route into China started from Western Asia eastward initially and then south-west and eastwards into Burma, Laos, Thailand and Vietnam (Payne, 1970) and probably into Malaysia. Using the classification of Payne (1970), these cattle can be categorised under the small short-homed or lyre-homed Zebu group like the series of breed of Bhutan, Nepal, Sikkim and northern India. The breed is a result of crossbreeding between the shorthorn-type cattle originating in west Asia and Zebu from India, notably the Kangyam and Ongole. The influence of the Ongole is probably more distinct, as the feature of this breed is quite prominent particularly in large populations of Kedah-Kelantan cattle. The breed has in general, the characteristics of beef cattle, but lacks the prominence and blocky conformation identifiable in such improved beef breeds as the Brahman or the Simental. However, this breed is relatively small and has a

6 compact body. Brown is the most predominant colour but dark brown, black and white colours are also found. The lack of distinctive beef characteristics is due to lack of selection pressure and their continued use as draught animals (Devendra et ai., 1973). 2.2 The Oestrous Cycle of Cows The oestrous cycle is defined as the time between periods of oestrus. The average length of oestrous cycle for cow is 21 days. However, individual variation can be seen in cows and cycles ranging from 17 to 24 days are considered normal. The duration of oestrus is 12-1 8 hours whilst the time of ovulation is 10-12 hours after the end of oestrus period (AUrich, 1994 ; Bernard et ai., 1983), respectively. 2.2.1 Stages of The Cow's Oestrous Cycle The stages of oestrous cycle are oestrus, metoestrus, dioestrus and proestrus. These stages occur in a cyclic and sequential manner, except for the period of anoestrus (absence of cycling) which occurs in seasonal breeders such as the ewe, doe, and mare in the temperate regions. In most species, anoestrus also occur during pregnancy and early postpartum period. Oestrus is defined as the period of time when the female is sexually receptive to the male and thus, will stand for mating. Oestrus lasts for 12 to 18 hours in cows. There is also considerable variation in the oestrous cycle among

7 individuals. Cows under hot environment have shorter period of oestrus (10 to 12 hours) compared to the average of 18-hour for cows under cool climates (Valle et al., 1994). There is a great variation amongst individual cows in the intensity of oestrus signs; the manifestation tend to be more marked in heifers than in cows. However, it is generally agreed that the most reliable criterion that a cow or a heifer is in oestrus is that she will stand to be mounted by another cow or a male (Williamson et ai., 1972; Esslemount and Bryant, 1974; Foote, 1975). In general, the female becomes more restless, irritable, and excitable during oestrus (Britt et al., 1986). In addition, increased vocalization and interest in the male will become apparent if the male is in the vicinity. Pelvic adjustment into a mating position may occur. Cows are unique in that they display rather strong homosexual tendencies, making oestrus detection comparatively easy even when bulls are not present. Cows in oestrus will solicit mounts and attempt to mount other cows (Unal et al., 1986). Cows that are coming into oestrus will mount cows that are in oestrus. However, mounting activity becomes more frequent when two or more cows are in oestrus than when a single cow is in oestrus (Britt, 1987). Frequency of mounting is higher at night than during the day, possibly because it is closer to the onset of oestrus; more mounting activity will be seen during early morning as compared to late afternoon (Pennington et al., 1985). The cervix is relaxed and the uterus becomes turgid. Clinical signs of oestrus are congested vulva and clear mucus discharge from the vulva.

The period of metoestrus begins with the cessation of oestrus and lasts 8 for about 3 days. Primarily, it is the period of corpus luteum formation. There is reduction in the amount of mucus secretion from the uterine, cervical and vaginal glands. However, ovulation occurs during this period in cows. Also, a phenomenon known as metoestrous bleeding occurs in some cows. During late proestrus and oestrus, high estrogen concentrations increase the vascularity of the endometrium. This vascularity reaches its peak about 1 day after end of oestrus. With declining estrogen level, some breakage of capillaries occurred, resulting in a small loss of blood (Sato et al., 1982) and thus, metoestrous bleeding. Dioestrus is characterized as the period in the cycle when the corpus luteum is fully functional. In the cow it starts about day 5 of the cycle, when an increase in blood concentration of progesterone can be detected, and ends with regression of the corpus luteum on day 16 or 17. The uterine glands undergo hyperplasia and hypertrophy, the cervix becomes constricted, the vaginal mucosa becomes pale and the secretions of the genital tract are scant and sticky. It has been called the period of uterine preparation for pregnancy (Geary and Reeves, 1992). Proestrus is characterized as the period in the cycle when the corpus luteum regresses and extends to the start of oestrus. The principal distinguishing feature of proestrus is the occurrence of rapid follicle growth. Late during this period the effect of estrogen on the duct system and behavioral symptoms of approaching estrus can be observed. The uterus enlarges; the endometrium