Medical entomology network MediLabSecure

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Medical entomology network MediLabSecure Presentation of the working group dedicated to medical and veterinary entomology (WP4) Medilabsecure "Heads of Lab" meeting 14 / 01 / 2015 Vincent ROBERT / Marie PICARD

> What is medical entomology? > Which objectives and activities for this working group? > Who are we? Presentation of the laboratory members > What s next? Setting up of the training modules

> What is medical entomology? > Which objectives and activities for this working group? > Who are we? Presentation of the laboratory members > What s next? Setting up of the training modules

Medical & Veterinary Entomology The branch of entomology dealing with arthropods, which affect the health and well-being of man and vertebrate animals the vectorial triad Schematic representation of the various health disciplines involved in vector borne disease (source: Vbornet) Perhaps the most basic concept in Med Vet Entomol

Surveillance vs. Monitoring Monitoring: procedures implemented for temporary and continuous observations (e.g. mosquito species population dynamics) and is not followed by any additional activities Surveillance: procedures developed in response to a risk and carried out to support subsequent actions (e.g. vector control)

> What is medical entomology? > Which objectives and activities for this working group? > Who are we? Presentation of the laboratory members > What s next? Setting up of the training modules

MediLabSecure objectives General objective Enhancing and strengthening the preparedness to common health threats and biosafety risks by the creation of a network of laboratories (human virology, animal virology, medical entomology). Specific objectives > Create a framework for collaboration to improve surveillance and monitoring of emerging vector borne viral diseases (arboviruses) and respiratory viruses > Provide training for public health experts > Improve integrated surveillance (animal, human, entomological) > Provide tools for awareness, risk assessment, monitoring and control of the different emerging viruses (transmission, spread, human impact )

Participating countries The Mediterranean and Black Sea regions

Organization WP1 / Coordination WP2 WP3 WP4 WP5 Animal virology Human virology Med. & Vet. entomology Public Health

Medical entomology working group (WP4) Purpose: To build a network of laboratory specialists in medical entomology, and more precisely, in mosquito vectors of arboviruses WP2 Animal virology WP3 Human virology and biosafety respiratory virus WP4 Medical entomology arbovirus

Target mosquito Arbovirus Diseases Dengue Chikungunya Rift valley Fever West Nile Fever Vector Aedes Culex

Activities / Timeline 1. Identification of laboratories 2. Inventory and assessments 3. Training modules 4.Public health impact of vectors Timeline 2014 2015 2016 2017

1) Identification of laboratories (2014) Contact data base / Communication tools On line questionnaire Committee selection Experts in human and animal health and medical entomology Establishment of the medical entomology network (MLS Entomo)

MLS / Network members (labs) Working group medical entomology (WP4)

2) Inventory and assessments (needs and gaps) > Estimation of needs and gaps of the different partners (with special consideration for efficacy of national surveillance systems devoted to mosquito vectors) > Inventory of present mosquito species (with special focus on invasive species) (2014 / 2015) Deliverable : > Expertise mapping: Identification of countries with expertise on medical entomology (M12) > Comprehensive checklist of mosquito species per beneficiary country (M24)

Training inventory on medical entomology 4 countries with specific medical entomology courses: Algeria, Egypt, Turkey, Serbia Serbia / Faculty of Agriculture, University of Novi Sad > Insect Systematics and Medical Entomology (2 months) > Urban Entomology (1 month) > Introduction to identification of blackflies (2 days) > Introduction to identification of mosquitoes (2 days) Algeria / Saad Dahlab University > Medical entomology (2 years) Turkey / Hacettepe University, HU ESRL VERG Laboratories > Genetic and phenotypic comparison of sand fly and mosquito populations using geometric morphometrics and barcoding methods with relevant softwares (12 days) Egypt/ Ain Shams University > Medical and Veterinary Entomology (1 semester) > Vector Biology and Control (5 days)

3) Capacity building (2015 / 2016) Training modules Deliverable : > Five days training modules in 3 geographical zones (one module per zone), using training manual (M31) Training tools Deliverable : > Electronic determination keys of mosquito species (on line) (M36)

4) Public health impact of vectors (2016 / 2017) > Risk assessment related to the vector presence > Help with appropriate preparedness and emergency response (isolated cases vs. large outbreak) Deliverable : > Regional workshop (M38) > Road map for harmonization of surveillance systems of mosquito vectors (M48) > Final report on the activities related to Public health impact of vectors, including an inventory of major vectors and potential vectors, needs and gaps (M48)

> What is medical entomology? > Which objectives and activities for this working group? > Who are we? Overview of the laboratory members > What s next? Setting up of the training modules

Presentation of the medical entomology lab network members > Analysis of the 1st questionnaire Medical and Veterinary Entomology laboratory capacities > Based on the results of the 19 selected laboratories (Medical Entomology network)

Methods Identification of laboratories On-line questionnaire Committee selection Establishment of the medical entomology network (MLS Entomo) > 36 lab candidates > 19 selected labs 40 questions laboratories capacities Studied data: General information Activities and services Influence of the laboratory Equipment and logistics Organization and training 1. Dominant diseases studied 2. Dominant vector studied 3. Collection / Identification 4. Equipment 5. Vector control

MLS / Network members Working group medical entomology (WP4) Bosnia &Herz. / Lab. Diagnostic Veterinary Institute of the Rep. of Srpska Kosovo / Institute of Vet. medecine Fac of Univ. of Prishtina, Fac of Agri and Vet. Serbia / Lab for med and vet entomology Fac of Agriculture, Univ. Novi Sad Moldova / Lab of systematics and molecular phylogeny Institute of zoology Ukraine / Montenegro / Lab for applied zoology Biotechnical faculty FYROM / Lab for virology and molecular diag Institue of Public Health Albania / Entomology lab Institute of Public Health Morocco / Lab. des Maladies Vectorielles Institut Pasteur Medical entomology lab Institut National d'hygiène Algeria / Eco epidémiologie parasitaire et génétique des populations Institut Pasteur Turkey / HU ESRL VERG (Vector ecology research group) Hacettepe Univ. Georgia / The R. Lugar Center for Public Health Research National Center for Disease Control and Public Health Armenia/ Reference lab center National Center for Disease Control Lebanon / Lab of immunology National Center for Disease Control and Public Health Jordan / Parasitic and Zoonotic Diseases Department Ministry of Health Tunisia/ Medical entomology lab Institut Pasteur Libya / Lab of parasitology and vector born diseases National Center for Disease Control Egypt / Entomology department and the Research and Training Center on Vector of Diseases Ain Shams Univ Palestine / Vector Control Unit Ministry of Health

MLS / Network members (contact) Kosovo / Kurtesh Sherifi Moldova / Tatiana Sulesco Bosnia &Herz. / Jelena Maric Serbia / Dušan Petrić Ukraine / Montenegro / Igor Pajovic Turkey / Bulent Alten FYROM / Elizabeta Jancheska Albania / Silvia Bino, Kela DIkolli Morocco / M'hammed Sarih (Institut Pasteur) Chafika Faraj (Institut National d'hygiène) Georgia / George Babuadze Armenia/ Lusine Paronyan Lebanon / Nabil Haddad Algeria / Zoubir Harrat Jordan / Khalil Kanan Tunisia/ Ali Bouattour Libya / Ahmed Elgrari Egypt / Abdallah Samy Palestine / Samir Sawalha MediLabSecure Team (WP4) Email: mls.entomo@ird.fr Discussion list: mls.entomo all@listes.ird.fr Vincent Robert Marie Picard

Main activities What are the main activities of our 19 labs? Medical Entomology 16 Veterinary Entomology 7 Bacteriology 4 Virology 11 Parasitology 13 Epidemiology 10 Public Health 11 Animal Health 4 Others 5 0 2 4 6 8 10 12 14 16 18

Research activities What kind of research activities? Taxonomy / Systematic 8 Morphology / Identification 16 Ecology 13 Distribution 16 Physiology 4 Genetic 7 Vector control 10 Insecticide 8 Transmission of pathogens 13 Other 2 0 2 4 6 8 10 12 14 16 18

Competences What is our matter of competences? Mosquito Culicinae 18 Mosquito Anophelinae 15 Tick Argasidae 8 Tick Ixodidae 12 Phlebotomine / Sandflies 14 Culicoides 4 Rodent 7 Other 7 0 2 4 6 8 10 12 14 16 18 20

Diseases What diseases are we involved in? West Nile Fever 15 Dengue 7 Chikungunya 5 Rift Valley Fever Crimean Congo Hemorragic Fever 6 6 Targeted diseases of the MLS lab. network Leishmaniasis Malaria Ricketsiosis Other (Borreliosis,Leprospirosis, plague ) 4 6 8 12 RVF 15% CCHF 15% WNF 39% DEN 18% CHIK 13%

Dominant disease studied > West Nile Fever (15/19 countries)

Collection Which laboratories are collecting mosquitoes in the field? no yes yes 17 89% no 2 11%

Identification Which laboratories are identifying mosquitoes? no yes yes 16 84% no 3 16% Traditional morphology 16 Morphometry 4 Molecular (e.g. PCR) 7 Biochemical (e.g. isoenzymology) Other: CD Rom 1

Vector control In which countries is there a vector control program?

> What is medical entomology? > Which objectives and activities for this working group? > Who are we? Overview of the laboratory members > What s next? Setting up of the training modules

Setting up of the training program Objective To develop capacity building in medical entomology and vector surveillance for laboratory staff of the MediLabSecure network. Activities > Five days training modules in three different areas, according to geography, climate and vector circulation > Tailored training modules > 2 persons (technician, scientific) of the selected laboratory staff (MediLabSecure network members)

Training program: which area? Zone 1 Zone 2 Zone 1 (7 countries): Albania Bosnia and Herzegovina Kosovo Montenegro Moldova Serbia The Former Yugoslav Republic of Macedonia Zone 2 (7 countries): Armenia Georgia Jordan) Lebanon Palestine Turkey (Ukraine) Zone 3 Zone 3 (5 countries): Algeria Egypt Libya Morocco Tunisia

Training program: when? 2015 2016 June 2015 Zone 1 Sept 2015 Zone 2 Spring 2016 Zone 3 Zone 1 Zone 2 Zone 3

Training program: where? Zone 1: Serbia, Novi Sad Zone 2: Turkey, Ankara Zone 3:??? Zone 1 Zone 2 Zone 3

Thank you