Name: Block: Muppet Genetics Lab Due: _ Introduction Much is known about the genetics of Sesamus muppetis. Karyotyping reveals that Sesame Street characters have eight chromosomes: three homologous pairs and one pair of sex chromosomes. pair #1 and #2 are the largest and have a centrally placed centromere. pair #3 is much shorter with the centromere at one end. Sex is determined by X and Y chromosomes just as it is in the related species, Homo Sapiens. Through the process of gene mapping, geneticists have found the specific locations of the genes for eye shape (A), nose color (B), fur length (C), body color (D), blood type (E), and hair type (F). By observing the offspring of many crosses, geneticists also have determined the types of inheritance that several of these genes exhibit. In this lab, you will determine the phenotype and genotype of seven Muppet characters, mate two characters, and produce two Muppet offspring. #1 #2 #3 X Y A C D E B F There are five parts: Part 1: Muppet Phenotypes and Genotypes Part 2: Muppet Mates Characteristics Part 3: Punnett Squares for Muppet Offspring Part 4: Muppet Offspring Characteristics Part 5: Muppet Family Portrait 1
Requirements Part 1: Muppet Phenotypes and Genotypes marks For each Muppet, identify the phenotype and possible genotype of each Muppet using Table 1.1 and Table 1.2 found on the website Part 2: Muppet Mates Characteristics Choose two Muppets (one male and one female) to mate Identify the phenotype, genotype and type of inheritance for each trait 4 marks Part 3: Punnett Squares for Muppet Offspring 12 marks Using your chosen Muppets, fill in Punnett Squares for each given trait Indicate the probabilities expressed as ratios and percent for each possible trait Part 4: Muppet Offspring Characteristics marks Create two Muppet Offspring (one male, one female) using the results of your Punnett Squares First offspring must have the most likely characteristics (i.e. highest probabilities) Second offspring may have any characteristics Part 5: Muppet Family Portrait marks Detailed drawing of Muppet parents and Muppet offspring Phenotypes of each Muppet should be able to be recognized from the drawing Drawn on.5 x 11 paper This lab is worth 40 marks and will be based on the requirements outlined above. The final information submitted should be neatly written in ink or typed. Handout will be on website if you wish to use a computer. Your lab grade will be based upon the completion of all requirements, neatness and organization. Evaluation rubric is on pages 7-. Rubric has detailed explanations on how marks will be distributed for each part of the lab. 2
Part 1: Muppet Phenotypes and Genotypes Using Table 1.1: Phenotypes and Genotypes of Muppets and Table 1.2: Meet the Muppets (on the website) fill in the phenotypes and possible genotypes for each Muppet. Phenotypes Sex Eye Shape Nose Colour Fur Length Body Colour Hair Type Blood Type Grover Elmo Zoe Telly Honker Rosita Cookie Monster Genotypes Sex Eye Shape Nose Colour Fur Length Body Colour Hair Type Blood Type Grover Elmo Zoe Telly Honker Rosita Cookie Monster 3
Part 2: Muppet Mates Characteristics Using a male and female Muppet from the list, record the phenotype and genotypes for each characteristic. These will be Muppet Parents that you will mate to create two Choosing Muppet Parents with different characteristics is preferred! Male Muppet: Characteristic Phenotype Genotype Type of Inheritance Eye Shape Nose Colour Fur Length Body Colour Hair Type Blood Type Female Muppet: Characteristic Phenotype Genotype Type of Inheritance Eye Shape Nose Colour Fur Length Body Colour Hair Type Blood Type 4
Part 3: Punnett Squares for Muppet Offspring Fill out the Punnett Squares in order to determine the possible traits of your Using the space below each Punnett Square, determine the probability for each phenotype. First, write the probability as a ratio (i.e. 1:4), then as a percent (i.e. 25%). Note: a Punnett Square for sex is not included! Muppet 1: Muppet 2: Eye Shape Nose Colour Fur Length Body Colour Body Colour Blood Type 5
Part 4: Muppet Offspring Fill out the two tables of characteristics of two possible offspring from the Punnett Squares. One Muppet Offspring should have the most likely characteristics (i.e. highest probabilities) from your Punnett Squares. The second Muppet Offspring can have any traits that were possible from your Punnett Squares. Muppet Offspring with different characteristics is preferred! Muppet Offspring #1 Name: _ Sex Eye Shape Characteristic Phenotype Genotype Probability of Trait (%) Nose Colour Fur Length Body Colour Hair Type Blood Type Muppet Offspring #2 Name: _ Sex Eye Shape Characteristic Phenotype Genotype Probability of Trait (%) Nose Colour Fur Length Body Colour Hair Type Blood Type Part 5: Muppet Family Drawing Create a family portrait of your Muppet Family. Drawings must meet these criteria: Portrait includes drawings of both Muppet Parents and both Muppet Offspring Phenotypes for visible traits (i.e. eye shape, body colour, etc.) should be identifiable from drawings Colour should be used accurately Label each Muppet with their name. Do not label the traits Drawing should be on a separate.5 x 11 paper If someone can look at your drawing and correctly identify the phenotype for each trait for all Muppets, then your drawing is good enough! Marks are awarded for identifying traits, not artistic ability. 6
Self-Evaluation with Rubric For each part of the assignment, highlight the criteria that you think your lab meets. Read each box carefully! Justify your choices in the self-reflection portion of the table. Reflect on what you learned, the process of the lab and what you liked/didn t like. Not yet meeting (1) Approaching (2) Meeting (3) Fully Meeting (4) Part 1: Muppet Phenotype & Genotype Multiple errors in identifying phenotypes & genotypes of all Some errors in identifying phenotypes & genotypes of all Minimal errors in identifying phenotype & genotype of all No errors in identifying phenotype & genotype of all Part 2: Muppet Mate s Characteristics Multiple errors in 1) Some errors in 2) Muppets have mostly similar traits. 1) Minimal to no errors in listing phenotype & genotype of two 2) Muppets have mostly similar traits. 1) No errors in & genotype of two 2) Muppets have mostly contrasting traits. Part 3: Punnett Squares for Muppet Offspring 1) Multiple errors in crossing genes. 2) Multiple errors in calculating probabilities for each phenotype. 3) Probability expressed as a ratio or a percent, or was not calculated. 1) Some errors in crossing genes. 2) Some errors in calculating probabilities for each phenotype. 3) Probability expressed as a ratio and/or a as percent. 1) Minimal or no errors in crossing genes. 2) Minimal or no errors in calculating probabilities for each phenotype. 3) Probability expressed as a ratio and as a percent. 1) No errors in crossing genes. 2) No errors in calculating probabilities for each phenotype. 3) Probability expressed as a ratio and as a percent. Part 4: Muppet Offspring Characteristics 1) Multiple errors in 2) First Muppet Offspring does not have most probable genotypes from Part 3. 3) Lack of variety in genotypes between 1) Some errors in 2) First Muppet Offspring has most probable genotype from Part 3. 3) Lack of variety in genotypes between 1) Minimal errors or no errors in listing phenotype and genotype of two 2) First Muppet Offspring has most probable genotype from Part 3. 3) Some variety in genotypes between 1) No errors in 2) First Muppet Offspring has most probable genotype from Part 3. 3) Lots of variety in genotypes between 7
Part 5: Muppet Family Portrait 1) Cannot identify any phenotypes for visual traits given to Muppet Offspring as listed in Part 4 from drawing. 2) Drawing lacks detail and/or colour. 1) Can identify some phenotypes for visual traits given to Muppet Offspring as listed in Part 4 from drawing. 2) Drawing lacks detail and/or colour. 1) Can identify majority of phenotypes for visual traits given to Muppet Offspring as listed in Part 4 from drawing. 2) Accurate use of colour and detail. 1) Can easily identify all phenotypes for visual traits given to Muppet Offspring as listed in Part 4 from drawing. 2) Accurate use of detail and colour. Reflection:
Part 1 Part 2 Part 3 Part 4 Part 5 TOTAL 4 12 40 Comments: Part 1 Part 2 Part 3 Part 4 Part 5 TOTAL 4 12 40 Comments: Part 1 Part 2 Part 3 Part 4 Part 5 TOTAL 4 12 40 Comments: 9