EXHIBIT E. Minimizing tick bite exposure: tick biology, management and personal protection

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EXHIBIT E Minimizing tick bite exposure: tick biology, management and personal protection

Arkansas Ticks Hard Ticks (Ixodidae) Lone star tick - Amblyomma americanum Gulf Coast tick - Amblyomma maculatum American dog tick - Dermacentor variabilis Winter tick - Dermacentor albipictus Black-legged tick - Ixodes scapularis Brown dog tick - Rhipicephalus sanguineus Soft Ticks (Argasidae) Fowl tick - Argus persicus Spinose ear tick - Otobius megnini

Hard tick Soft tick

Three-host tick life cycle

Lone star tick, Amblyomma americanum Distribution From Texas throughout south-central and southeastern US. Range has expanded Abundance in an area is influenced by habitat, host availability and other factors (natural enemies)

Very abundant in AR, aggressive feeder, readily bites humans Woodlands and woodland edges Three-host tick; 1 year life cycle Adults most abundant in May, June, July; appear in March Nymphs abundant in April or May and August Larva (seed) ticks abundant in late summer Alpha-gal allergy, ehrlichiosis, STARI (southern tick associated rash illness - Borrelia), Heartland virus, Ricketttsia parkeri, and tularemia; cytauxzoonosis (bobcat fever) in domestic cats Lone Star Tick

Engorgement of adult female lone star through time

How important are white-tailed deer as tick hosts? Lone star and black-legged tick populations are expanding geographically and in density, especially in areas with increasing deer herds Other ticks species do use deer as hosts but not at the frequency of the lone star and black-legged ticks; lone star larvae, nymphs and adults readily feed on white tailed deer

Gulf Coast Tick Now established in Arkansas Three-host tick Long host list: large and small mammals, birds Diseases Heartwater disease in Africa (Cowdria ruminantium), also transmits Rickettsia parkeri (a newly described spotted fever); Hepatozoon americanum (American canine hepatozoonosis dog ingests infected tick)

American dog tick, Dermacentor variabilis Distribution Along the Atlantic coast, New England to Florida, west to Montana and west Texas Was once more common than the lone star tick

American Dog Tick Three-host tick, 1-2 year life cycle, depending on conditions Bites occasionally cause tick paralysis Diseases: Rocky Mountain spotted fever, tularemia, anaplasmosis in cattle, possibly cytauxzoonosis (bobcat fever) in domestic cats Once more commonly encountered than the lone star tick

Black-legged tick, Ixodes scapularis Distribution Eastern North America, Atlantic coast, mid-west and Gulf coast region

Black-legged Tick Three-host tick, 1 year life cycle in Arkansas Adults feed on deer cattle, horses, etc. Immature ticks feed on birds, small mammals and lizards Vector of Anaplasmosis, Babesiosis, and Lyme disease (Northeast, northcentral and mid-atlantic) Readily feed on deer

Brown dog tick, Rhipicephalus sanguineus Distribution Global occurrence between 50 degrees North and 30 degrees South Preferred host dog, opportunistic on other animals

Minimizing Tick Bite Exposure Avoid Tick Infested Areas Wear Proper Clothing Use Tick Repellents Routine Self-Checking Controlling Ticks in the Environment Controlling Ticks on Hosts

Repellents Clothing repellents (permethrin) Skin repellents (DEET) Effective non-deet clothing and skin repellents are available (Ex. BioUD (biopesticide isolated from wild tomato) has shown repellent efficacy against lone star and American dog ticks)

Clothing and Self-Checking Wear light colored clothing, tuck in shirt tail and pants into socks Inspect yourself frequently for ticks (at least twice daily), examine clothing and body twice daily when in infested areas Prompt removal of ticks, the longer a tick is attached, the greater the chance of it transmitting a pathogen (6-24 hrs.) If you are bitten, record the date of the tick bite

Grasp the tick with tweezers Tick removal Pull upward with steady pressure, do not jerk or twist the tick. If mouthparts remain in the skin, remove them with a sterilized needle (similar to removing a splinter). Write down the date of the tick bite. Removed ticks can be preserved in a vial with alcohol Wash hands and bite area thoroughly with soapy water then apply an antiseptic such as alcohol

Tick Control in the Environment Broadcast application of insecticides/acaricides not feasible for large areas, used in yards and public use areas shorter-term management option Control weeds and brush, keep lawn mowed, remove debris and leaf litter around the yard, especially near woods line reduces humidity of microclimate lowering tick survival reduces harborage for wildlife that bring ticks into the area Exclude wildlife from yard (fencing out deer not easy) Tick control on pests can reduce tick abundance in yards Controlled burns can reduce lone star tick populations, may cake a couple of years for reduction to become apparent

Tick Control on Important Hosts Deer self-application device 4-poster feeder station Deer contact acaricide treated paint rollers when consuming corn, treats ears, head and neck Studies in the NE show black-legged tick reductions Tennessee study showed a reduction in lone star ticks; the cost per station was about $20 per month; one station would cover 40-50 acres Feeding systemic insecticides to deer Feeding ivermectin-treated corn to deer reduced lone star tick abundance (TX and TN studies). Also effective against cattle fever ticks