Practical manual for small scale dairy farmers in Vietnam. Reproductive Management in Dairy Farms

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Practical manual for small scale dairy farmers in Vietnam Reproductive Management in Dairy Farms Hanoi 008

Practical manual for small scale dairy farmers in Vietnam Reproductive Management in Dairy Farms (Second edition) Composed and Published by Distributed by Hanoi 008

Forewords The Vietnam Belgium Dairy Project (VBDP) aims to increase the income of the rural population by sustainable growth of the domestic milk production in Vietnam. The project is implemented from 005-009 by the Ministry of Agriculture and Rural Development (MARD) with technical assistance of the Belgian Technical Cooperation (BTC). Comprehensive training of farmers is one of the main activities of the project. The project has chosen for a Training of Trainer system (TOT) in which leading demonstration farmers and/or technicians are trained to become a trainer of a group of dairy farmers. This booklet on Reproductive Management in Dairy Farms is a part of a series of booklets that cover the different aspects of Good Dairy Farming Practice. Each booklet is accompanied by flipcharts that can be used during training sessions. All manuals and flipcharts can be downloaded from the Dairy Vietnam Website: www.dairyvietnam.org.vn readers and most importantly to convince farmers to follow the instructions in the manual. We would like to thank all persons who contributed to the completion of this second edition. Specials thanks go to Dr. Nguyen Tan Anh and Dr. Berend De Leeuw. On this occasion, we would also like to express our gratitude and appreciation to the farmers and technicians who follow the guidelines of the manuals and who teach other farmers by using our publications. Sincere thanks! Constructive feedback on any of our publication is always welcome! Although some knowledge and concepts might be unfamiliar to some dairy farmer in Vietnam, the authors made very short and simple expressions which are accompanied by animated and easy to understand images to intrigue

THIS BOOKLET IS COMPLETED UNDER THE COOPERATION OF: Vietnam Belgium dairy project F, No 4, Thuy Khue str., Tay Ho dist., Hanoi, Vietnam Tel: (+84) 4 7 344 78 Fax: (+84) 4 7 344 79 E-mail: vbdairyproject@vnn.vn Content Chapter The Importance of Reproductive Management...3 Department of Livestock production No, Ngoc Ha str. Ba Dinh dist., Hanoi, Vietnam Tel: (+84) 4 7 345 443 Fax: (+84) 4 8 443 8 / (+84) 4 8 436 80 E-mail: cn@mard.gov.vn Belgian technician cooperation F7-F9, No 4, Thuy Khue str., Tay Ho dist., Hanoi, Vietnam Tel: (+84) 4 7 80 57 Fax: (+84) 4 7 80 57 E-mail: vietnam@btcctb.org Dairy Vietnam F, No 4, Thuy Khue str., Tay Ho dist., Hanoi, Vietnam Tel: (+84) 4 7 346 46 Fax: (+84) 4 7 344 79 Email: info@dairyvietnam.org.vn Website: www.dairyvietnam.org.vn www.nganhsuavn.org.vn Chapter Heat...9 Chapter 3 Artificial Insemination...5 Chapter 4 Pregnancy and Calving...43 Chapter 5 Reproductive management...6 Author: Raf Somers, Ngo Tien Dung, Luu Viet Vien and Didier Tiberghien Illustration by Mr. Dang Duc Tinh Designed by Compass Co., Ltd. Tel: (+84.4) 69 676

Why do dairy cows give birth When dairy cows give birth: milk and calf

Chapter The Importance of Reproductive Management

Dairy farming is a business The main income from dairy farming comes from milk Milk Production Curve Milk yield (kg/day) Peak 0 3 4 5 6 7 8 9 0 Month of lactation Chapter : The Importance of Reproductive Management Figure : Milk Production Curve Cows start to produce milk immediately after calving. The milk production reaches a peak from week 4 to week 0 after calving, then goes down gradually in the following months The highest yield (average yield / day) can be reached when the cow has 0 calving/year (305 day in milk + 60 days dry) 5

Chapter : The Importance of Reproductive Management Cow : Has 4 lactations in 4 years ( calving/year) Lactation : 4.000 kg/lactation, Lactation : 4.00 kg/lactation, lactation 3: 4.00 kg/lactation, Lactation 4: 4.000 kg/lactation. Total in 4 years: 6.300 kg Milk production (kg/day in 4 years) = 6.300 / (4 x 365) =,6 kg/day 4.000 kg Days in milk Dry 4.00 kg 4.00 kg 4.000 kg Dry Dry Dry Days in milk Days in milk Days in milk Cow : Has 3 lactations in 4 years Lactation : 4.00 kg/lactation, Lactation : 4.300 kg/lactation, lactation 3: 5.000 kg/lactation. Total in 4 years: 3.500 kg Milk production (kg/day in 4 years) = 3.500 / (4 x 365) = 9,5 kg/day Calving interval: 365 days (idealy) Chapter : The Importance of Reproductive Management 4.00 kg 4.300 kg 5.000 kg Dry Dry Days in milk Days in milk Days in milk 4 years Average milk production/day of cow higher than of cow of,9 kg Dry To achieve this, dairy cows should be successfully inseminated within 85 days after calving. However, this is a hard-to-do task. Therefore, farmers and inseminators should work closely together to detect heat and seminate in time in order to shorten calving intervals 6 7

Chapter : The Importance of Reproductive Management Milk yield (kg/day) Calving 0 3 4 5 6 7 8 9 0 month 85 days (Inseninate) Days in milk (305 days) Preqnant: 80 days Calving interval: 365 days (idealy) Figure : Calving Interval Dry period (60 days) Calving Note: the Calving Interval is calculated as the interval between 0 successive calvings. Chapter Heat The longer the calving interval is, the lower the average dairy milk production will be The longer the dry period is, the longer you have to feed a cow that does not produces milk Therefore, to shorten the calving interval, farmers should: - Give good care to the cows - Pay attention to heat detection - Call the AI technical in time - Keep clear records 8

. What is heat? Heat is the period of acceptance for mating in cows Heat occurs only in non-pregnant heifers and cows The period of receptivity lasts between 6 to 30 hours The average interval between 0 heats is days, but it can vary from 8 to 4 days Chapter : Heat. What is causing heat In non-pregnant cows, the ovaria are producing the eggs and sexual hormones Every days, a mature egg is released from ovaria. Just before releasing the egg, hormones are released that cause the heat signs Note: A hormone is a substance produced by an organ in the body and released in the blood stream that gives signals to certain organs to increase or decrease a certain activity

Chapter : Heat 3. Signs of Heat Heat detection calls for the observation of changes. Changes in the vulva Pink and swollen vulva. Clear mucous discharge is visible 3 Chapter : Heat The changes can be classified in: - Changes of the vulva - Changes of the behavior of the cow Observe changes in the vulva. Closely check, use light to detect mucous discharge on floor at night. Very important to notice discharge on tail and udder 4 If mucous discharge is found, mark the cow and check again in the next morning Note: Mucous discharge should be distinguished from pus (unclear, bad smell) 5 Observation Signs of changes High pregnant cows can sometimes also have clear mucous discharge Pink swollen vulva 3 4 Mucous discharge on the floor at night 5 3

Chapter : Heat Changes in behavior Frequency of heat 0 Chapter : Heat Observation of changes in behavior 5 Dairy cows in heat show signs of seeking or following bulls or other cows. For good heat detection, cows should be let to roam freely at least 30 minutes in the morning and 30 minutes in the afternoon. If a farmer has just a few cows, he/she can work together with other farmers. 0 5 0 :00 6:00 0:00 4:00 8:00 :00 0:00 4:00 8:00 :00 6:00 0:00 4:00 Time Figure3: Frequency of heat Note: Heat occurs more during the night than during the day. (the reason is not known). At right there is more quietness in the stable and also the temperatures are lower Heat detection should also be done in the early morning, late afternoon or even during the night 4 5

Chapter : Heat Change of behavior Bellows frequently Chapter : Heat Is nervous and restless Behaves otherwise and fight other cows Refuse social contact with other cows Sniff vulvas or urine of other cows 3 4 Turns in circle or press her chin on the back of other cows Mount on other cows Allow other cows to mount her (standing heat) Cow eat less and produce less Frequent peeing 5 6 Frequent peeing 3 Fussing Excited Bellowing Refuse others coming for flirting or mounting 5 4 6 6 7

Chapter : Heat Note: if a cows stand immobile and allows other cow to mount her, you can be sure she s in heat. End of heat At the end of the heat, some blood might appear on the vulva Cows return to a normal status Chapter : Heat Vulva returns to normal, fine wrinkles appear like in a non-heat period 3 3 Vulva sniffing 3 Mounting other cows Cows eat less and produce less 3 8 9

Chapter : Heat 4. Heat Detection Good heat detection starts with good routine! What should we do when cow are found in heat? Do full recording in farm record Chapter : Heat Regular time to look for heat - Changes in the vulva - Changes in behavior Always take record when symtoms are observed Check the records to know which cow will come in heat (8-4 days heat interval) You can choose to record: - On a board in the barn (write it down in a book afterwards) - In a cow file (one file per cow) - In a heat recording book (one file for the herd) Which system you use is not important, but you should know when any cow was in heat! 0

Chapter : Heat Inform inseminator Possible reasons for no heat No ovulation + no heat Chapter : Heat No heat observed Ovulation + no heat Heat detection Inform inseminator. There is really no heat: There was heat, but not seen or recorded A No ovulation and no heat Ovarian disease Reproductive organ disease The cow has recently calved and the reproductive cycle has not started again. Hormonal imbalance. This is often due to 3

Chapter : Heat - Poor nutrition - Heat stress The cow is pregnant Improve feeding can influence cow s productivity and body conditions. B There is ovulation, but the cow does not show heat signs (silent heat). This can occur in the first cycle after calving. There was actually heat: Farmer did not see it - Insufficient heat detection - Very short heat period (<6 hours), possible in the night The farmer did see it, but did not record and forgot about it Chapter 3 Artificial Insemination 4

Definition Artificial Insemination (AI) is a technique by which semen is introduced artificially into the body of the uterus at the time of heat in an attempt to cause pregnancy. Chapter 3: Artificial Insemination 7

Chapter 3: Artificial Insemination Advantages of AI It provides opportunity to select high quality bull It minimizes the risk of spreading reproductive diseases It reduces the costs and the risks of keeping bulls at the farm Artificial insemination The quality of the bull and the semen is checked Frozen semen can inseminate thousands of cows per year Frozen semen can be stored for years and can easily be transported Semen of dead or that are far away bulls can be used for insemination Natural service A bull is the best possible heat detector Fertility rates are mostly better A bull can service only 0 cow per time How to do AI? Semen collection Healthy bull High fertility High genetic potentials 3 Chapter 3: Artificial Insemination Frozen semen can be stored for years and can be easily transported Natural service Semen collection 3 4 4 8 9

Chapter 3: Artificial Insemination Semen quality check (evaluation) Volume of semen V (ml) Activity A (%) Concentration C (tr/ml) Rate of abnormal sperm cells Skillful inseminators are required Chapter 3: Artificial Insemination Treatment and storage The semen is diluted The semen put in a straw The semen is frozen The semen is stored in liquid nitrogen (-96 O C) transport of frozen semen to insemination site. 30 3

Chapter 3: Artificial Insemination Selection of semen Improve genetics The straw with the semen is defrozen and warmed before the insemination. When to do AI? AI is done when the cow is in heat. Chapter 3: Artificial Insemination When the cow is in heat, the inseminator introduces the semen into the cow s uterus with specialized tools. 3 33 Note: The hardest task in AI is to determine the time for insemination (see Chapter - Heat detection). After insemination (natural service or by AI), the cow will get pregnant if sperm goes to the right place, at the right time From 0- hours after heat time, eggs will be released and survive for about 6- hours. Meanwhile, sperm can survive in uterus for about 4 hours. Therefore, the rule of morningafternoon should be followed

Chapter 3: Artificial Insemination When to Service a Cow in Heat Coming into heat 8 hours (0-4h) Standing heat 6 hours (3-30h) Going off heat 8 hours ( - 4h) Application of the morning-afternoon rule Chapter 3: Artificial Insemination If the cow is in heat in the morning, inseminate her in the afternoon If the cow is in heat in the afternoon, inseminate her in the next morning Artificial Insemination Natural Service 0 6 8 4 giờ Too early Too early Good Best Best Good Too late Too late Note: If a cow inseminated the day before is still in heat, inseminate her again Figure 4: Timing of insemination or natural service for cows in heat 34 35

Chapter 3: Artificial Insemination Data recording Farmers and inseminators record the data in order to predict the next heat to inseminate of the right time and to determine which semen should be used. What do farmers and inseminators record? Farmers should record the date and the signs of heat. Heat Insemination Pregnant diagnosis Ear-tag Date of Date of insemination period Next heat Semen ID heat Retal P.D. DAG6468 8/5 BD 5 9/5 5-/06 4/8.................. Chapter 3: Artificial Insemination Data recording book No Ear-tag Date of heat Sign Next heat period Note Inseminators should record full data in their books and farm record books: Cow ID (ear-tag) Date of heat Date of insemination Semen ID Date for pregnant diagnosis Next heat period 36 37

Chapter 3: Artificial Insemination Causes of low conception rates In fact, even when the heat is well detected and the insemination is done in time, the conception rate is not 00% (for AI, it is 60% after 0 service; for natural service it is 80%). In good farms, the number of services/conception is.7-.5. Example Low quality semen: Improper storage of semen Chapter 3: Artificial Insemination In farm, 30 insemination were done in 5 cows in year 5 cows now are pregnant, this gives a service/ cow: 30/5 = and conception rate of 5/30 = 0.5 ( or 50%) Unskillful inseminator Low conception rates may be due to: Improper timing of service - Insemination too early in the heat period - Not servicing a cow that is in heat - Servicing a cow that is not in heat Note: Conception rates in heifers are usually better 38 39

Chapter 3: Artificial Insemination Cow s conditions - Reproductive diseases - Hormonal disorder - Obstructed oviducts - Anatomical defects - Early embryonic death (cow becomes pregnant but pregnancy is not maintained) Farmers should Do good farm management including: - Feeding and water - Hygiene - Animal welfare - Avoid heat stress Chapter 3: Artificial Insemination To get high conception rate Farmers and inseminators should do their jobs well. - Enough light in the stable (be able to read a newspaper without problems) Detect heat and call inseminators in time Inseminators should be: - Skillful - Ensure good storage of semen - Inseminate at the right time Provide enough food for cow Avoid heat stress 40 4

Chapter 4 Pregnancy and Calving

. Pregnancy diagnosis check There are 0 ways to check conception: Heat detection: If the cow does not come in heat after 8-4 days after the insemination Pregnancy check by rectal palpation: 65-70 days after insemination (a skillful inseminator can do this after 45 days) Chapter 4: Pregnancy and Calving No signs of heat 8 to 4 days after the insmenation farmers should observe if the cow is whether or not coming into heat. If the cow is pregnant, there will be no sign of heat Cow inseminated After 8-4 days Heat detection In heat Inseminate again Not in heat cow might be pregnant 45

Chapter 4: Pregnancy and Calving If a cow is not in heat for 60 days after insemination, the cow might be pregnant However, the absence of heat can have other reasons (see page 3, 4) Rectal palpation Rectal palpation is only done by a technician (inseminator or veterinarian) only. In the early days of pregnancy, diagnosis via rectal palpation can be done but does not provide accurate results. From 70 days after insemination, technicians can diagnose pregnancy, the accuracy of results is good. Embryonic death The embryo dies at a young stage You will not observe anything, but the cow will come in heat again Abortion: abortion is the expulsion of fetus out of cow s body before the end of the pregnancy. The rate of abortion is about 3%-5%, the main reasons are: Infectious diseases The insemination is done when cow is already pregnant Physical injuries Chapter 4: Pregnancy and Calving The cow eats feed which contain toxins Inseminator inseminates pregnant cow Abortion Note: In most cases, the cause of abortion is unclear Pregnancy period The average pregnancy period of cows is 80 days (ranging from 70 to 90 days). During her pregnancy, the cow is not in heat. 46 47

Chapter 4: Pregnancy and Calving Calving Calving is the expulsion of the calf and placenta. Signs of calving The enlargement of the udder due to the production of colostrum Chapter 4: Pregnancy and Calving Relaxation of the pelvic ligaments, swelling of the vulva, mucous discharge - The cervix dilates and which allows mucous to come out - The cow delivers the calf a few hours after cervix dilates completely 48 49

Chapter 4: Pregnancy and Calving Normal fetus position Calving process: 03 stages NORMAL CALF POSITION 3. The expulsion of the placenta The placenta is expelled from the uterus. After the delivery of the calf, uterine contraction are still continuing for a period of time. Normally after 5-6 hours, expulsion is completed 3 Call a veterinarian if the placenta still retains after 6 hours 4 5 3 Chapter 4: Pregnancy and Calving. Dilation of the cervix: This stage lasts: -3 hours for mature cows 4-6 hours for heifers. Delivery of the calf The calf is expelled through the uterus. At this stage, the calf may still be in the water bag. When the water bag breaks, the head passes through the birth canal, the rest of the body demands little extra efforts to be expelled. This stage lasts -0 hours Note: a common mistake is to assist by pulling the forelegs of the calf, this is not necessary in normal cases. Note: Many farmers refuse to inseminate the cow in the period of 0-60 days after calving, because the cow is in the top of its milk production. This is one of the biggest mistakes you can make as a dairy farmer! Cows should have one calf per year. The insemination and the pregnancy do not influence the milk production 4 5 50 5

Chapter 4: Pregnancy and Calving Post calving The uterus shrinks and restores in size Head can return as early as 0 days after calving 90% of cows are in heat at least once within 60 days after calving Note: The st insemination should takes place between 0 and 60 days after calving b. How to deal with difficult calving: call the veterinarian or skilful farmer Note in handling - Wash and disinfect your hands, arms and the cow s vulva - Disinfect equipment - The position of calf should be checked before you start pulling the calf out. Traction should only be applied as the cow pushes Chapter 4: Pregnancy and Calving Post calving complications. Difficult calving Experience and judgment is necessary to decide when to assist calving. If after or hours of intense pushing, the calf s forefeet do not appear and the cow is exhausted, assistance should be provided. ABNORMAL CALF POSITIONS If the farmer does not have experience in dealing with difficult calving, a technician should be called immediately. a. Causes of difficult calving Weak pushing The calf is too big The position is abnormal The calf is death 5 53

Chapter 4: Pregnancy and Calving. Retained placenta : Retained placenta accounts for 5% to 30% and mostly after a difficult calving When the placenta is retained, a veterinarian should be called - The placenta should not be removed manually because it can harm the uterus Chapter 4: Pregnancy and Calving ABNORMAL CALF POSITIONS - Medicines should be used to avoid infection and stimulate uterus contraction Prevention of retained placenta is a critical part of reproductive management. It includes - Proper hygiene during calving - Proper nutrition during the dry period - Frequent roaming 54 55

Chapter 4: Pregnancy and Calving 3. Infections Infections are caused by microorganisms. Symptoms Mucous liquid comes out from vulva, this is normal till 5 days after calving and it can vary from water clean to red to even brown and green, as long as it does not stink or is thick yellow and unclear it is not a problem The cows get fever only in 0% of the cases Not eating My cow is infected, I have to call a veterinarian now! Caring of a newly born calf Make sure the calf is breathing Clear the nose and mouth Put cold water in the head of the calve If necessary hang calve upside down Put calve in dog sitting position, which gives both breast side the opportunity to inhale easily Turn the calf from one side to the other to make the fluid from the lungs come out Chương 4: Chửa và đẻ Not starting up in production Disinfect navel cord Infection can lead to infertility Use a bandage to stop the bleeding of the navel cord only when absolutely necessary touch the umbilical cord with your fingers Use Iodine solution (7%) to disinfect, pot dip or blue spray Call the veterinarian immediately Check the navel cord for infection (hurt, swelling) days after calving Call the veterinarian if necessary 56 57

Chapter 4: Pregnancy and Calving Colostrum milk Colostrum contains antibodies that help buiding up immunity against diseases The newly born calf should be fed colostrum as soon as possible, not later than hour after calving The volume of abomasum of the newborn calf is only.5 litter. So the amount of colostrum fed at one should not exceed.5 kg. Otherwise it will cause diarrhoea After birth, the calf should be removed from the mother cow Chapter 4: Pregnancy and Calving The amount of colostrum fed should be 0% of the body weight at birth The temperature of the colostrum is very important, so feed colostrum immediately after milking Keep the calf in individual, clean and dry cage and hutch Note: The left over of colostrum can be stored in the refrigerator for week perfectly in the freeze compartment. Before it is used, it need to warmed up in a bainmarie (39 O C) Direct heating will destroy the proteins Ear tag immidiately after birth Dehorn 0-30days after birth Remove extra teats 58 59

Chapter 5 Reproductive management

To ensure good reproductive management on the farm, technicians and farmers should work together. Farmers: Ensure good dairy farming practice Observe heat in time Call inseminators and veterinarians in time Chapter 5: Reproductive management Inseminators: Should be skillful Semen should be of high quality Ensure good storage of semen If everything above is fulfilled, every months, cow will have calf. Good farm management Inseminator check cow in heat 63

Chapter 5: Reproductive management The signs of heat are easy to see when: The cow is healthy and free from reproductive diseases The cow is not injured The cow does not suffer from stress (incl. heat stress) Feeding is sufficient and balanced The cow doesn t has difficult calving The cow can roam freely Raising cows with more than 90% HF blood (HF, F4, F5...) is difficult in most regions of Vietnam. These cows suffer more from heat stress than F (50% HF) and F (75% HF) In hot and humid condition, HF cow will show less heat signs this might result in long calving interval Areas like Lam Dong and Moc Chau are more suitable to raise HF cows Chapter 5: Reproductive management Many farms in Vietnam: Cows often suffer from heat stress Feeding for dairy cows is not balanced in nutrition Cows are very skinny Cows are restricted within barns 4/4hours 3 3 For these cows it can be difficult to show heat and get pregnant. Healthy cows Cows are free to roam Cows suffer from heat stress 3 3 4 an F cow an F cow an F3 cow a pure HF cow 4 64 65

Chapter 5: Reproductive management Some reproductive management indicators Indicator For individual cow Age at first calving Calving Interval Return to heat after calving (st heat) No. of insemination per conception Days of dry period Days between calving and conception For herd Average calving interval Heat after calving Insemination after calving Cows return to heat within 60 days after calving No. of insemination per conception Rate of heifers get pregnant after 0 service Rate of mature cows get pregnant after 0 service Rate of mature cows have to do 03 services Days of dry period Interval between calving and next pregnancy Rate of cows with interval between calving and next pregnancy > 0 days Unit Month Month Day Number Day Day Month Day Day % Number % % % Day Day % Goal < 4 < < 40 <.7 50-60 < 85 < < 40 < 45 90 <,7 > 65 > 50 < 0 50-60 85-0 < 0 Limit > 30 > 4 > 60 >.5 < 45 or > 70 > 40 > 4 > 60 > 60 < 90 >,5 < 60 < 40 > 0 < 45 or > 70 >40 > 45 The importance of data recording Farmers should do data recording in order to get critical reproductive data for themselves and the technicians. Farmers should record data into recording books and cow files. If farmers and technicians record all the reproductive data carefully, problems will be identified and solutions can be found efficiently. Record table in the barn Recording book 3 Chapter 5: Reproductive management 3 Farm profile 66 67

Chapter 5: Reproductive management Full data recording allows: Identify the time when the cow will get in heat and when it will calve Identification of the day the cow should be dried off Calculation of technical criteria Criteria of the dairy herd should be applied at the farm, district or province level or for the herd in the region that the inseminator serves. Farm indicators will be calculated - For a farm - For a region - For an inseminator Great. My cow got pregnant the cows were inseminated on March. It is 5 April today. Is there any cow in heat again? No Chapter 5: Reproductive management Key indicators: For heifers: Age at the first calving Ideally < 4 months and limit not more than 30 months Heat detection and insemination should start from months Heifers should be at least 350kg when inseminated For cows: Day between calving and insemination st insemination should take place within: Ideally before 45 days after calving and limit not later than 60 days after calving Day between calving and pregnant: Successful insemination should take place: Ideally before day 85 after calving and limit not later than 40 days after calving 68 69

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