OIE Reference Laboratory Reports Activities Activities in 2013 This report has been submitted : 2014-01-28 09:07:56 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Brucellosis (Brucella abortus) Address of laboratory: 175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, 430-757, KOREA (REP. OF) Tel.: +82-31 467.17.65 Fax: +82-31 467.17.78 e-mail address: brjung@korea.kr website: www.qia.go.kr Name (including Title) of Head of Laboratory (Responsible Official): Dr. Yong Ho Park DVM, MS, PhD QIA Commissioner Name (including Title and Position) of OIE Reference Expert: Suk-chan Jung Which of the following defines your laboratory? Check all that apply: Governmental Research OIE Reference Laboratory Reports Activities, 2013 1
ToR: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally RBT 2356 SAT 281 ELISA 281 FPA 281 Dip-stick 61 Direct diagnostic tests Nationally Internationally PCR 26 32 MLVA 58 32 Bacterial culture 26 32 ToR: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? NOTE: Currently, there are 22 laboratories that produce Standard Reference Reagents officially recognised by the OIE for 19 diseases/pathogens. Please click the following link to the list of OIE-approved International Standard Sera: http://www.oie.int/en/our-scientific-expertise/veterinary-products/reference-reagents/. If the reagent is not listed on this page, it is NOT considered OIE-approved. The next two questions allow you to indicate non-oie-approved diagnostic reagents. 2 OIE Reference Laboratory Reports Activities, 2013
Supply imported OIE-approved SRR Select where you import from list: Disease Test Available from Bovine brucellosis Indirect and Competitive enzyme-linked immunosorbent assay; Complement fixation; Agglutination; Buffered Brucella antigen tests; Fluorescence polarisation assay Dr Judith A. Stack AHVLA Weybridge, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom Tel: (44-1932) 35.76.10 Fax: (44-1932) 35.72.16 judy.stack@ahvla.gsi.gov.uk Type of reagent available Related diagnostic test Produced/ Supply imported Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg) Name of recipient OIE Member Countries RBT antigen RBT supply imported CFT antigen CFT supply imported MRT antigen MRT supply imported SAT antigen SAT supply imported 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? OIE Reference Laboratory Reports Activities, 2013 3
Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients RBT antigen RBT Produced 30 ml 30 ml 1 Africa Americas Asia and Pacific Europe Middle East SAT antigen SAT Produced 7048 ml Africa Americas Asia and Pacific Europe Middle East CFT antigen CFT Produced 100 ml Africa Americas Asia and Pacific Europe Middle East PCR test kit (multiplex) PCR Produced 576 tests Africa Americas Asia and Pacific Europe Middle East 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? 4 OIE Reference Laboratory Reports Activities, 2013
ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Name of OIE Member Country seeking assistance Date (month). samples received for provision of diagnostic support. samples received for provision of confirmatory diagnoses MONGOLIA April 9 MONGOLIA June 22 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided THAILAND Production of Brucella selective medium and application of multiplex differential PCR kit In loco MONGOLIA Training for phage typing for differentiation of Brucella species In loco ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? Title of the study Duration Purpose of the study Partners (Institutions) Distribution of Brucella species from a variety of animals and the molecular epidemiological analysis by MLVA assay in Mongolia 3 years (Dec. 2011 ~ v. 2014) Identifying Brucellosis in Mongolia and epidemiological correlation between Brucella isolates Institute of Veterinary Medicine The molecular epidemiological analysis of Brucella species from a variety of animals and application on diagnostics for Brucella canis 3 years (Oct. 2013 ~ Sep. 2016) Identifying Brucellosis in Thailand and epidemiological correlation between Brucella isolates between two countries and application and evaluation on serologic diagnostics of B. canis National Institute of Animal Health OIE Reference Laboratory Reports Activities, 2013 5
ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 6 (1) Ji-Yeon Kim, Moon Her, Sung-Il Kang, Kichan Lee, Hyang Keun Lee, and Suk Chan Jung (2013). Epidemiological relatedness between Brucella abortus isolates from livestock and wildlife in South Korea. J Wildl Disease 49(2), 2013, pp. 451 454 (2) Hu Suk Lee, Moon Her, Michael Levine, George E. Moore (2013). Time series analysis of human and bovine brucellosis in South Korea from 2005 to 2010. American J of Pre Med 110(2): 190-197. (3) Sung-il Kang, Moon Her, Ji-Yeon Kim, Kichan Lee, Jong-Wan Kim, Hyang-Keun Lee, So-Ra Sung, Hyorim Cho, SukChan Jung. Rapid, sensitive and specific detection of Brucella canis by real-time PCR assay using HybProbe. (2013). J Prev Vet Med 37(1) :24-28. (4) Hyang-Keun Lee, Jong-Wan Kim, Kichan Lee, Moon Her, Ji-Yeon Kim, Sung-il Kang, SukChan Jung (2013). Immunological analyses for recombinant GroEL of Brucella canis. J Prev Vet Med 37(1) : 29-34. (5) Jin Ju Lee, Dae Geun Kim, Dong Hyeok Kim, Hannah Leah Simborio, Wongi Min, Hu Jang Lee, Moon Her, Suk Chan Jung, Masahisa Watarai, Suk Kim. Interplay between clathrin and Rab 5 controls the early phagocytic trafficking and intracellular survival of Brucella abortus within HeLa cells. (2013) J Biol Chem. 288(39):28049-28057. (6)Jin Ju Lee, Jae Hong Kim, Dae Geun Kim, Dong Hyeok Kim, Hannah Leah Simborio, Won Gi Min, Man Hee Rhee, Jong Hwan Lim, Hong Hee Chang, Suk Kim. Characterization of betaine aldehyde dehydrogenase (betb) as an essential virulence factor of Brucella abortus. Vet Microbiol. Epub j.vetmic.2013.10.007. b) International conferences: 5 (1)Ji-Yeon Kim, So-Ra Sung, Moon Her, Kichan Lee, Sung-Il Kang, Hyang-Keun Lee, Hyorim Cho and Suk-Chan Jung (2013). Immunoproteomic analysis and identification of antigens to minimize serological cross-reaction for bovine brucellosis (94th CRWAD in Chicago) (2)So-Ra Sung, Ji-Yeon Kim, Moon Her, Kichan Lee, Jeong Hui Gu, Sung-Il Kang, Hyang Keun Lee, Suk Chan Jung. (2013). Serological study on Brucellosis in livestock of Korea (94th CRWAD in Chicago) (3)Hyang-Keun Lee, Hyorim Cho, Moon Her, Ji-Yeon Kim, Kichan Lee, Sung-Il Kang, So-Ra Sung, Suk-Chan Jung. DNA vaccine encoding chaperonin GroEL protein fused to cytokine genes protects Brucella canis (94th CRWAD in Chicago) (4)Kichan Lee, Moon Her, Ji-Yeon Kim, Sung-Il Kang, Erdenebaatar Janchivdorj, Suk-Chan Jung (2013). Isolation of Brucella species from human and livestock of Mongolia (66th Brucellosis Research Conference in Chicago) (5)Sung-Il Kang, Moon Her, Hyorim Cho, Ji-Yeon Kim, Kichan Lee, Hyang Keun Lee, So-Ra Sung, Suk Chan Jung (2013) MLVA typing of the Brucella strains isolated from the animal and human in (66th Brucellosis Research Conference in Chicago) c) National conferences: 6 (1)Ji-Yeon Kim, So-Ra Sung, Moon Her, Kichan Lee, Sung-Il Kang, Hyang-Keun Lee, Hyorim Cho and Suk Chan Jung (2013). Detection of immunogenic proteins of Brucella abortus RB51 strain by two-dimensional electrophoresis. (Spring, KSVS) (2)So-Ra Sung, Ji-Yeon Kim, Moon Her, Kichan Lee, Jeong Hui Gu, Sung-Il Kang, Hyang Keun Lee, Suk Chan Jung (2013). Serological monitoring on brucellosis in livestock of Korea (Spring, KSVS) (3)Hyorim Cho, Hyang-keun Lee, Kichan Lee, Moon Her, Ji-Yeon Kim, Sung-Il Kang, So-Ra Sung, Suk Chan Jung (2013). DNA vaccine encoding omp31-rpll fusion gene enhances protective response for Brucella canis (Spring, KSVS) (4)Sung-Il Kang, Moon Her, Hyorim Cho, Ji-Yeon Kim, Kichan Lee, Hyang Keun Lee, So-Ra Sung, Suk Chan Jung (2013). Development of a specific PCR assay for Brucella canis (Spring, KSVS). 6 OIE Reference Laboratory Reports Activities, 2013
(5)Hyang-Keun Lee, Hyorim Cho, Kichan Lee, Jong-Wan Kim, So-Ra Sung, Sung-Il Kang, Ji-Yeon Kim, Moon Her, Suk Chan Jung. (2013). DNA vaccine encoding out membrane protein 28 of Brucella canis elicits humoral and cellmediated immune responses in mice (Spring, KSVS). (6)Hyang-Keun Lee, Jong-Wan Kim, Hyo Rim Cho, Kichan Lee, Ji-Yeon Kim, Moon Her, Sung-Il Kang, So-Ra Sung, Suk Chan Jung. (2013) Development of DNA Vaccine Candidates fused to genetic adjuvant for Canine Brucellosis (Autumn, KSVS) d) Other: (Provide website address or link to appropriate information) 0 none ToR: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 2 b) Seminars: 1 c) Hands-on training courses: 19 d) Internships (>1 month): 0 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country a Mongolia 5 a Thailand 4 b Mongolia 15 c Cambodia 1 c Indonesia 2 c Lao 2 c Malaysia 2 c Mongolia 2 c Myanmar 2 c Nepal 1 c Philippines 2 c Singapore 1 c Thailand 2 c Vietnam 2 OIE Reference Laboratory Reports Activities, 2013 7
ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO/IEC 17025 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited MRT RBT SAT ELISA FPA PCR Bacterial culture Accreditation body 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1) ToR: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? 8 OIE Reference Laboratory Reports Activities, 2013
Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented 66th annual Brucellosis Research Conference 12/13 Chicago, USA 2 presenting posters 1) Isolation of Brucella species from human and livestock of Mongolia 2)MLVA typing of the Brucella strains isolated from the animal and human in Mongolia 94th CRWAD 12/13 Chicago, USA 3 presenting posters 1)Immunoproteomic analysis and identification of antigens to minimize serological cross-reaction for bovine brucellosis 2)Serological study on Brucellosis in livestock of Korea 3)DNA vaccine encoding chaperonin GroEL protein fused to cytokine genes protects Brucella canis ToR: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? OIE Reference Laboratory Reports Activities, 2013 9
te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: http://www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3 Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries Evaulating multiplex PCR kit (differentiating Brucella species 2 Africa Americas Asia and Pacific Europe Middle East ToR: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? Kind of consultancy Location Subject (facultative) Ad hoc Group meetings France Revision of the OIE health code on brucellosis Review of OIE manual France A revised draft of Chapter X.X.X Infection with Brucella abortus, melitensis and suis, and Chapter X.X.X Infection with Brucella ovis 25. Additional comments regarding your report: 10 OIE Reference Laboratory Reports Activities, 2013