VPH dept. Laboratory for Food Microbiology Andrea Humski, Ph.D, DVM Marina Mikulić, DVM
about CVI... Established in 1933. Leading national diagnostic, research and scientific institution in the field of veterinary medicine -Ministry of Agriculture, Fishery and Rural Development (MPRRR) -Ministry of Science, Education and Sports (MZOS)
AREAS OF OPERATION 1. DIAGNOSTIC AND ANALYTICAL ACTIVITIES Preparedness, field and laboratory diagnostics, profilaxis, surveillance and monitoring, data analysis, planing and proposal of preventive actions, reference and scientific advisory functions in control of: Infectious diseases Zoonoses Food and feed safety Veterinary drugs and residues
2. SCIENTIFIC AND RESEARCH ACTIVITIES Performed within the framework of The National scientific programme Executed through the several scientific research projects financially supported by Ministry of science, education and sport
CVI laboratory network...employees total no. 238 PhD 34 Msc 13 Sci. advisor 7 Senior sci. associate - 10 Sci. associate 11
CP Križevci Vinkovci
CVI Zagreb I. VPH Dept. I.1. Laboratory for food microbiology I.2. Laboratory for residue control I.3. Laboratory for feed microbiology I.4. Laboratory for analytical chemistry I.5. Laboratory for veterinary medicinal products
LABORATORY FOR FOOD MICROBIOLOGY, Zagreb microbiological control of food: official control (import/export), on request (domestic production/market) monitoring programmes microbiological contamination of meat in slaughterhouses microbiology food safety of products- veterinary inspection on border posts research and scientific work
LABORATORY FOR FOOD MICROBIOLOGY ACCREDITATION CERTIFICATE Microbiological parameter/method 1 Salmonella spp. HRN EN ISO 6579:2003 / cor 2004 2 Salmonella spp. TECRA VIA Screening method AOAC Official Method 989.14;998.09 3 Listeria monocytogenes Part 1.: Detection method HRN EN ISO 11290-1:1999 4 Listeria monocytogenes Part 2.: Enumeration method HRN EN ISO 11290-2: 1999 5 Listeria spp. TECRA VIA Screening method AOAC Official Method 995.22;2002.09 6 Enumeration of microorganisms - CCT at 30 C HRN ISO 4833:2003 7 Escherichia coli Part 2.:Colony count technique at 44 C HRN ISO 16649-2:2001 8 Escherichia coli Part 3.: MPN method ISO/TS 16649-3:2005 9 Escherichia coli O157 HRN EN ISO 16654:2003 10 E. coli O157. TECRA VIA screening method AOAC RI, L.NO.001101 11 Enterobacteriaceae Part 1.: MPN method ISO 21528-1:2004 12 Enterobacteriaceae Part 2.: Colony count technique ISO 21528-2:2004 13 Staphylococcus aureus and other species Part 1.: Technique using Baird-Parker medium HRN EN ISO 6888-1:2004 14 Sulfite-reducing bacteria HRN ISO 15213:2004 15 Clostridium perfringens - Colony count technique HRN EN ISO 7937: 2005 16 Campylobacter spp. Part 1. Detection method ISO 10272-1:2006 17 Campylobacter spp. Part 2. Enumeration method ISO 10272-2:2006 18 Campylobacter spp. TECRA VIA Screening method
External Quality Assesment/Proficiency Testing Health Protection Agency, London, UK (since 2005.) Parameter No. of tested samples Salmonella spp. 15 Listeria monocytogenes / E 8 Listeria monocytogenes / D 4 E. coli, MPN 9 E. coli / E 2 E. coli O157 3 ACC 11 Campylobacter spp. 6 Coag. + Staphylococcus / E 5 Staph. aureus enterotoxin 2 Cl. perfringens / E 3 Enterobacteriaceae / E 3
Present and future activities.
Zoonoses in humans 2005-2008 Disease 2005 Cases Deaths Rate per 100,000 population Rank Salmonellosis 5619 0 124.9804 1 Leptospirosis 126 0 2.8026 2 Q fever 40 0 0.8897 3 Toxoplasmosis 39 0 0.8675 4 Trichinellosis 27 0 0.6005 5 Disease 2006 Cases Deaths Rate per 100,000 population Rank Salmonellosis 4734 1 105.2958 1 Trichinellosis 45 0 1.0009 2 Leptospirosis 39 0 0.8675 3 Toxoplasmosis 31 0 0.6895 4 Q fever 28 0 0.6228 5 Disease 2007 Cases Deaths Rate per 100,000 population Rank Salmonellosis 3331 0 74.0897 1 Campylobacteriosis 141 0 3.1362 2 Leptospirosis 63 1 1.4013 3 Q fever 43 0 0.9564 4 Toxoplasmosis 31 0 0.6895 5 Disease 2008 Cases Deaths Rate per 100,000 population Rank Salmonellosis 3664 0 81.4964 1 Campylobacteriosis 1294 0 28.7818 2 Q fever 41 0 0.9119 3 Brucellosis 25 0 0.5561 4 Toxoplasmosis 24 0 0.5338 5
6000 5500 5000 4500 4000 3500 3000 2500 2000 1500 1000 500 0 Salmonellosis Campylobacteriosis 2005 2006 2007 2008
1. Monitoring programme - Ordinance on conduction of veterinary inspection and control of animal products in traffic across the border of the Republic of Croatia (O.G. 132/08) / Ordinance on the document for registration of veterinary health inspection of shipments of animal origin entering the Republic of Croatia (O.G.129/06 and 75/07) Annex II 2. Baseline study acc. to Commission Decision 2007/516/EC: Campylobacter and Salmonella prevalence in broiler flocks (carcasses + caecal content)
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