ACUP 602.01 Rodent Health Monitoring Program The intent of this Animal Care and Use Procedure (ACUP) is to describe the CARE Rodent Health Monitoring Program. This ACUP is intended for animal care staff and CARE veterinary staff. This ACUP is approved by the Cornell Institutional Animal Care and Use Committee (IACUC). Any exemption must be approved by the IACUC prior to its application. TABLE OF CONTENT 1. Introduction 2. Materials 3. Procedures 4. Personnel Safety 5. Animal Related Contingencies 6. References 7. Appendix 1. Introduction The rodent health monitoring program measures pathogen status of rodents housed at Cornell University. Health monitoring results provide objective results for the biosecurity efforts designed to prevent the rodent populations from exposure to infectious pathogens. Historical health monitoring data are available on the CARE webpage. 2. Materials Equipment for collecting blood. Equipment for endo and ectoparasites evaluation. Rodent transport containers. 3. Procedures a. General: i. For monitoring the population of rodents in breeding and experimental units, monitor only populations housed for more than 4 6 weeks. ii. Use outbred females as sentinels placed at the beginning of the monitoring period when about 4-6 weeks of age. iii. House the sentinels in the same manner as the population, and ensure that they receive the same bedding, food and water. iv. Maintain and sample a minimum of two sentinel mice per sentinel cage. More sentinels can be housed in the same cage to serve as backups. v. One sentinel cage can monitor up to 80 cages.
vi. At every bedding change, contaminate sentinel cages with about 2-5 mls of dirty bedding from every monitored cage. b. CARE veterinary/technical staff: i. Monitor for specific pathogens and sample at the specific frequencies as outlined in the appendix of this document. ii. Collect perianal tape, fur pluck, and fecal samples for in house evaluation. iii. Collect serum samples and send to IDEXX-RADIL (Research Animal Diagnostic Laboratory University of Missouri) for clinical profiles as defined in the appendix of this document. iv. Ship live animals to IDEXX-RADIL for basic and comprehensive profiles. v. When presented with a sick or dead sentinel, send serum to IDEXX-RADIL, if possible, and submit fresh, or fresh fixed, tissues for histopathologic examination. vi. Review IDEXX-RADIL results and promptly inform the principal investigators and facility managers of unanticipated results. Note: Specific diagnostic panel composition may vary with unique animal colony parameters (e.g., closed breeding colony) and are tailored by the facility CARE veterinarian. c. CARE webmaster: post diagnostic results to the web.. 4. Personnel Safety Medical emergencies: call 911 When working with animals, wear appropriate PPE, observe proper hygiene, and be aware of allergy, zoonosis, and injury risks. Refer to the CARE Occupational Health and Safety webpage for more information. 5. Animal Related Contingencies Post contact information for emergency assistance in a conspicuous location within the animal facility. Emergency veterinary care is available at all times including after working hours and on weekends and holidays: Biomedical settings: CARE (pager 1-800-349-2456) Non-emergency veterinary questions & requests for care, call or email CARE veterinary staff at 607-253-4378 or care@cornell.edu, respectively. 6. References: CARE Health Monitoring webpage: http://www.research.cornell.edu/care/health.html CARE Occupational Health and Safety webpage. http://www.research.cornell.edu/care/ohs.html Rev #/Author: Rev #/Effective Date : Review Date : Referee: SOP :.01: J. Gourdon,.01 : Nov 2003 Aug 2013 M. Martin CARE602.01
7. Appendix Mouse Schedule Breeding (Clinical ) Cornell Panel 3 (Basic profile) Cornell Panel 2 (Annual sentinel sreening) Clean Suites Non terminal serology In-house Submit live animals Non terminal serology In addition to panel 4 Send fecal samples to Charles River for Pinworm, Pasteurella (10 pooled pellets/35-40 cages), and Helicobacter PCR* In house fur pluck Submit live animals ------------------ Accepted Pathogen Suites ------------------------ Non terminal serology In-house -------------------------- (Basic profile) serology (no live animals submitted) In-house ---------------------------- Non terminal serology In-house ------------------------ Cornell Panel 2 (Annual sentinel sreening) Submit live animals serology In-house serology In-house parsitology (RADIL) EDIM, MHV, MNV, Parvo NS-1 (MPV, MVM), Mycoplasma pulmonis, Sendai, TMEV GDVII (CARE-In House): fecal flotation and perianal tape (endoparasites), fur pluck (ectoparasites)
All testing done at RADIL Ectromelia, EDIM, LCM, Mycoplasma pulmonis, MHV, MNV, Parvo NS-1 (MPV, MVM), PVM, Reo3, Sendai, TMEV GDVII Cornell Panel 3 (Basic profile) Klebsiella oxytoca, Klebsiella pneumoniae, Pasteurella pneumotropica, Pseudomonas aeruginosa, Salmonella spp.; All testing done at RADIL Cornell Panel 2 (Annual sentinel screen) CARB, Encephalitozoon cuniculi, Ectromelia, EDIM, LCM, Mycoplasma pulmonis, MAD1, MAD2, MCMV, MHV, MNV, Parvo NS-1 (MPV, MVM), Polyoma, PVM, Reo3, Sendai, TMEV GDVII, Tyzzer's PCR Specimen: lymph node-mpv fecal pellets- MNV nasopharyngeal swab- Mycoplasma pulmonis Citrobacter rodentium, Corynebacterium kutscheri, Klebsiella oxytoca, Klebsiella pneumoniae, Mycoplasma pulmonis, Pasteurella pneumotropica, Pseudomonas aeruginosa, Salmonella spp., Streptobacillus moniliformis, Streptococcus spp. (Grp A, B, C), Streptococcus pneumoniae; Lesioned Organs Rat Schedule Breeding Basic necropsy profile + PCR + Microbiology Comprehensive necropsy profile + PCR + Microbiology
Clinical profile (RADIL) Mycoplasma pulmonis, Parvo NS-1 (H-1, KRV, RPV, RVM), PVM, RCV, Sendai, TMEV-like virus (AHDL/CARE) fecal flotation and perianal tape (pinworms). All testing done at RADIL Basic Necropsy plus PCR, Microbiology, and Histopath options LCM*, Mycoplasma pulmonis, Parvo NS-1 (H-1, KRV, RPV, RVM), PVM, REO3*, RCV, Sendai, TMEV-like virus, MAD 1*, Mycoplasma pulmonis, Pasteurella pneumotropica, Salmonella spp.*, Streptococcus pneumoniae*; Lesioned organs PCR Helicobacter spp*. Comprehensive necropsy profile plus PCR, Microbiology, and Histopath options All tests done at RADIL CAR bacillus, HANT, LCM, MAD1, Mycoplasma pulmonis, Parvo NS-1 (H-1, KRV, RPV, RVM), PVM, REO3, RCV, Sendai, TMEV-like virus, TYZZ Bordetella bronchiseptica, Citrobacter freundii, Corynebacterium kutscheri, Pasteurella pneumotropica, Pseudomonas aeruginosa, Salmonella spp., Streptococcus pneumoniae, beta hemolytic Streptococcus spp. (not Group B); PCR Helicobacter spp. * These tests performed only on breeding colonies Hamster
Schedule Breeding parasitiology parastiology + 2 sentinels/<80 cages Comprehensive profile + + PCR + Microbiology parasitiolgy + Clinical profile + (RADIL) LCM, PVM, Sendai, Tyzzer s (AHDL) fecal flotation (RADIL) E. cuniculi, LCM, PVM, REO3, Sendai, SV5, Tyzzer s Comprehensive profile + microbiology + + histopathology (AHDL) Enteric: Campylobacter spp., Clostridium spp., Lawsonia spp., Salmonella spp. Tracheal wash: Pasteurella spp., Streptococcus pneumoniae PCR (RADIL) Helicobacter spp. (AHDL) (fecal float, direct smear) Guinea Pig Schedule Breeding Basic profile Comprehensive plus profile Clinical (RADIL) serology profile E. cuniculi, PI3, PVM, Sendai
(AHDL) fecal flotation All tests done at RADIL E. cuniculi, LCM, PI3, PVM, Sendai, SV5, TYZZ Basic profile Bordetella bronchiseptica, Salmonella spp., Streptococcus pneumoniae; Comprehensive plus profile All tests done at RADIL E. cuniculi, GPCMV, LCM, PI3, PVM, Sendai, SV5, TYZZ Bordetella bronchiseptica, Salmonella spp., Streptococcus pneumoniae;