OIE Reference Laboratory Reports Activities

OIE Reference Laboratory Reports Activities Activities in 2015 This report has been submitted : 2016-01-15 19:44:47 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Salmonellosis Address of laboratory: Viale delluniversità, 10, 35020 Legnaro (Padova) ITALY Tel.: +39-049 8084.296 Fax: +39-049 8830.268 E-mail address: aricci@izsvenezie.it Website: www.izsvenezie.it Name (including Title) of Head of Laboratory (Responsible Official): Antonia Ricci Name (including Title and Position) of OIE Reference Expert: Antonia Ricci, Head of food safety Department at Istituto Zooprofilattico Sperimentale delle Venezie Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities, 2015 1

ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally 0 0 Direct diagnostic tests Nationally Internationally Isolation and identification 2030 0 Serotyping - slide agglutination 1650 328 Phage-typing 900 57 MIC (micro-plate method) 308 150 PCR and Real Time PCR 1060 18 PFGE 200 108 MLVA 240 0 Molecular serotyping 480 20 Whole Genome Sequencing 10 0 Salmonella confirmatory test 500 200 ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 2 OIE Reference Laboratory Reports Activities, 2015

3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Name of OIE Member Country seeking assistance Date (month). samples received for provision of diagnostic support. samples received for provision of confirmatory diagnoses NIGERIA June 0 400 VIETNAM January 0 170 TANZANIA July 0 30 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? OIE Reference Laboratory Reports Activities, 2015 3

Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided VIETNAM Improve technical skills on Salmonella diagnosis remote NIGERIA Provide support on management of epidemiological data on Salmonella and on laboratory analyses aimed to chacterize Salmonella strains remote ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 16 1) L. Barco, S. Belluco, A. Roccato, and A. Ricci, A systematic review of studies on Escherichia coli and Enterobacteriaceae on beef carcasses at the slaughterhouse, Int. J. Food Microbiol., vol. 207, pp. 30 39, Aug. 2015. 2) L. Barco, F. Barrucci, E. Cortini, E. Ramon, J. E. Olsen, I. Luzzi, A. A. Lettini, and A. Ricci, Ascertaining the relationship between Salmonella Typhimurium and Salmonella 4,[5],12:i:- by MLVA and inferring the sources of human salmonellosis due to the two serovars in Italy, Front. Microbiol., vol. 6, Apr. 2015. 3) S. Belluco, L. Barco, A. Roccato, and A. Ricci, Variability of Escherichia coli and Enterobacteriaceae counts on pig carcasses: A systematic review, Food Control, vol. 55, pp. 115 126, Sep. 2015. 4) S. Belluco, L. Barco, A. Roccato, and A. Ricci, Escherichia coli and Enterobacteriaceae counts on poultry carcasses along the slaughterline: A systematic review and meta-analysis, Food Control, vol. 60, pp. 269 280, Feb. 2016. S. Belluco, V. Cibin, R. Davies, A. Ricci, and A. Wales, A review of the scientific literature on the control of Salmonella spp. in food-producing animals other than poultry. 2015. 5) A. De Cesare, K. Krishnamani, A. Parisi, A. Ricci, I. Luzzi, L. Barco, A. Lucchi, A. Miccolupo, and G. Manfreda, Comparison between Salmonella enterica Serotype Enteritidis Genotyping Methods and Phage Type, J. Clin. 4 OIE Reference Laboratory Reports Activities, 2015

Microbiol., vol. 53, no. 9, pp. 3021 3031, Sep. 2015. 6) E. Cortini, F. De Pasquale, A. Longo, and E. Ramon, Metodi innovativi per la caratterizzazione dei Patogeni Alimentari, La Settim. Vet., no. 906, pp. 31 33, 2015. 7) L. Gerzova, V. Babak, K. Sedlar, M. Faldynova, P. Videnska, D. Cejkova, A. N. Jensen, M. Denis, A. Kerouanton, A. Ricci, V. Cibin, J. Österberg, and I. Rychlik, Characterization of Antibiotic Resistance Gene Abundance and Microbiota Composition in Feces of Organic and Conventional Pigs from Four EU Countries, PLoS One, vol. 10, no. 7, p. e0132892, Jul. 2015. 8) S. Iannetti, E. Malizia, A. Sorgente, A. Ricci, V. Cibin, and P. Colangeli, The Integrated Management System for Salmonella Control Plan in Italy, J. Heal. Med. Informatics, vol. 06, no. 04, 2015. 9) C. Losasso, V. Cappa, M. L. Neuhouser, V. Giaccone, I. Andrighetto, and A. Ricci, Students Consumption of Beverages and Snacks at School and Away from School: A Case Study in the rth East of Italy, Front. Nutr., vol. 2, Oct. 2015. 10) M. Mancin, L. Barco, C. Saccardin, and A. Ricci, Proposed statistical analysis to evaluate qualitative proficiency testing of Salmonella serotyping, Accredit. Qual. Assur., vol. 20, no. 4, pp. 305 310, Aug. 2015. 11) G.M. or Uddin, M.H. Larsen, L. Barco, T. Minh Phu, A. Dalsgaard Clonal Occurrence of Salmonella Weltevreden in Cultured Shrimp in the Mekong Delta, Vietnam PLoS One, vol. 10, Jul. 2015 12) A. Pezzuto, C. Losasso, M. Mancin, F. Gallocchio, A. Piovesana, G. Binato, A. Gallina, A. Marangon, R. Mioni, M. Favretti, and A. Ricci, Food safety concerns deriving from the use of silver based food packaging materials, Front. Microbiol., vol. 6, Oct. 2015. 13) Y.T. Proroga, F. Capuano, M.R. Carullo, I. La Tela, R. Capparelli, L. Barco, V. Pasquale Occurrence and antimicrobial resistance of Salmonella strains from food of animal origin in southern Italy, Folia Microbiol.(Praha), vol. 61, Jan. 2016 14) A. Ricci, M. T. Da Silva Felicio, L. Vivas-Alegre, P. Butaye, R. H. Davies, T. Hald, A. Havelaar, B. A. Lindstedt, M. C. J. Maiden, E. M. Nielsen, G. Scavia, M. Struelens, J. Threlfall, and D. L. Baggesen, Molecular typing methods for major food-borne microbiolgical hazards and their use for attribution modelling, outbreak investigation and scanning surveillance, in Options Méditerranéennes, vol. Series A:, no. 111, 2015, pp. 59 66. 15) A. Roccato, M. Uyttendaele, V. Cibin, F. Barrucci, V. Cappa, P. Zavagnin, A. Longo, P. Catellani, and A. Ricci, Effects of Domestic Storage and Thawing Practices on Salmonella in Poultry-Based Meat Preparations, J. Food Prot., vol. 78, no. 12, pp. 2117 2125, Dec. 2015. 16) A. Roccato, M. Uyttendaele, V. Cibin, F. Barrucci, V. Cappa, P. Zavagnin, A. Longo, and A. Ricci, Survival of Salmonella Typhimurium in poultry-based meat preparations during grilling, frying and baking, Int. J. Food Microbiol., vol. 197, pp. 1 8, Mar. 2015. b) International conferences: 4 1) A. Roccato, M. Uyttendaele, L. Barco, V. Cibin, F. Barrucci, I. Patuzzi, and A. Ricci, Domestic Handling of Chicken Carcasses: Quantification of Campylobacter Species Cross-contamination., in IAFP 2015, 2015, p. 62. 2) A. Roccato, V. Cibin, F. Barrucci, M. Favretti, A. Piovesana, A. Pezzuto, M. Dal Cin, S. De Rui, and A. Ricci, Riskbased control options to mitigate microbial risks associated to traditional fermented Italian salami and soppresse consumption., in 2nd EFSA Scientific Conference, 2015, p. 112. 3) A. Roccato, M. Uyttendaele, F. Barrucci, V. Cibin, M. Favretti, A. Piovesana, A. Pezzuto, M. Dal Cin, S. De Rui, and A. Ricci, Artisanal Italian Salami and Soppresse: Identification of Control Strategies to Manage Microbiological Risks., in IAFP 2015, 2015, p. 183. 4) A. Roccato, M. Uyttendaele, V. Cibin, F. Barrucci, V. Cappa, P. Zavagnin, A. Longo, and A. Ricci, Domestic Consumer-style Storage and Thawing Practices: Effect on Salmonella in Poultry-based Meat Preparations., in IAFP 2015, 2015, p. 184. c) National conferences: 1 1) A.A. Lettini, V. Cibin, F. De Pasquale, M. Mancin, I. Patuzzi, K. Antonello, D. Conficoni, A. Ricci. Occurrence of extended-spectrum β-lactamase (ESBL) - producing Escherichia Coli isolated from Italian poultry farms and fresh poultry meat. Oral Presentation. SIDiLV, Montesilvano (PE) 30/09-2/10-2015. d) Other: (Provide website address or link to appropriate information) 1 Reports about the Italian passive surveillance system of Salmonella from veterinary samples about the period 2011-2012 and 2013-2014 http://www.izsvenezie.it/salmonella-report-entervet-2011-2012-2013-2014/ OIE Reference Laboratory Reports Activities, 2015 5

ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 1 b) Seminars: 1 c) Hands-on training courses: 1 d) Internships (>1 month): 1 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country a Vietnam 10 b Vietnam 30 c Nigeria 1 d Nigeria 1 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO 17025 Certificate scan (PDF, JPG, PNG format) certificate-iso-17025.pdf 16. Is your laboratory accredited by an international accreditation body? 6 OIE Reference Laboratory Reports Activities, 2015

Test for which your laboratory is accredited Isolation and identification of Salmonella on primary production samples Serotyping of Salmonella strains Salmonella phage-typing PCR to differentiate S. Typhimurium and monophasic variant (S. 4,[5],12:i:-) Serotyping of S. Enteritidis and S. Typhimurium strains Real time for detection of Salmonella spp. on food and feed matrices Accreditation body 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2014, Chapter 1.1.3a) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? National/ International Title of event Co-organiser Date (mm/yy) Location. Participants International Final OIE Twinning Project CVVI 03/2015 Nha Trang, Vietnam 34 19. Did your laboratory participate in scientific meetings on behalf of the OIE? ToR 10: To establish and maintain a network with other OIE Reference Laboratories OIE Reference Laboratory Reports Activities, 2015 7

designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: http://www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3 8 OIE Reference Laboratory Reports Activities, 2015

Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries Assess Laboratory Capability on Salmonella isolation from veterinary samples collected at primary production level (organizer) 76 Assess laboratory capability on Salmonella serotyping(organizer) 11 Global Microbial Identifier (GMI) Proficiency Test 2015 Assess laboratory capability on Salmonella isolation from animal faeces according ISO rules 36 Assess laboratory capability on Salmonella Enteritidis and Salmonella Typhimurium phage-typing 7 Assess laboratory capability on Salmonella serotyping 34 Assess laboratory capability on Salmonella sub-typing by PFGE 10 ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: OIE Reference Laboratory Reports Activities, 2015 9

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