OIE Collaborating Centres Reports Activities

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OIE Collaborating Centres Reports Activities Activities in 2016 This report has been submitted : 2017-01-20 17:44:12 Title of collaborating centre: Maladies infectieuses de la reproduction en Europe Address of Collaborating Centre: Laboratoire National de Contrôle des Reproducteurs (LNCR) French national laboratory for health controls in breeding stock 13 rue Jouet 94704 Maisons-Alfort cedex FRANCE Tel.: +33-(0)1 43.53.51.00 Fax: +33-(0)1 43.53.51.01 E-mail address: edith.authie@lncr.org Website: www.lncr.org Name of Director of Institute (Responsible Official): Name (including Title and Position) of Head of the Collaborating Centre (formally OIE Contact Point): Name of writer: OIE Collaborating Centres Reports Activities, 2016 1

ToR: To provide services to the OIE, in particular within the region, in the designated specialty, in support of the implementation of OIE policies and, where required, seek for collaboration with OIE Reference Laboratories ToR: To identify and maintain existing expertise, in particular within its region 1. Activities as a centre of research, expertise, standardisation and dissemination of techniques within the remit of the mandate given by the OIE Epidemiology, surveillance, risk assessment, modelling Title of activity Contribution to surveillance of SBV re-emergence in 2016 in France Transmission of PEDv via reproduction Scope Data extraction and analysis from the LNCR database on SBV seroconversions in breeding bulls To assess the transmission of PEDv via semen from infected boars via experimental studies in collaboration with ANSES Diagnosis, biotechnology and laboratory Title of activity Scope Laboratory diagnostics of infectious reproductive diseases and other diseases of relevance for international trade of breeding animals and their gametes Approx. 250 000 tests were carried out (of which 80% were serological tests, mainly in the breeding sector Semen quality assessment in breeding males About 10 000 tests (all methods included) were carried out for bull and boar semen quality assessment Participation to proficiency tests organized by reference laboratories ANSES (6), GD Animal Health (7), CODA-CERVA (12) Provision of consultancy to the French ministry of agriculture Provision of consultancy to Service du développement rural de la Polynésie française Development and validation of an interferon gamma PCR assay for the diagnostics of bovine tuberculosis Detection of Campylobacter fetus and Trichomonas fetus in the context of weanlings export to third countries Presence and significance of bovine leukemia virus DNA in semen from seropositive bulls Study initiated in 2012 and completed in 2016; report submitted to funding body (French ministry of agriculture). ToR : To propose or develop methods and procedures that facilitate harmonisation of international standards and guidelines applicable to the designated specialty 2. Proposal or development of any procedure that will facilitate harmonisation of international regulations applicable to the surveillance and control of animal diseases, food safety or animal welfare Proposal title Scope/Content Applicable area 2 OIE Collaborating Centres Reports Activities, 2016

Detection of Porcine Epidemic Diarrhoea virus in semen A comparison of RT-PCR methods for PEDv detection in semen was carried out in collaboration between LNCR and ANSES- Ploufragan Surveillance and control of animal diseases Food safety Animal welfare Detection of Orthobunyaviruses in bovine semen Development of a generic RT-PCR for detection of orthobunyaviruses in bull semen Surveillance and control of animal diseases Food safety Animal welfare ToR: To establish and maintain a network with other OIE Collaborating Centres designated for the same specialty, and should the need arise, with Collaborating Centres in other disciplines ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other centres, laboratories or organisations 3. Did your Collaborating Centre maintain a network with other OIE Collaborating Centres (CC), Reference Laboratories (RL), or organisations designated for the same specialty, to coordinate scientific and technical studies? No 4. Did your Collaborating Centre maintain a network with other OIE Collaborating Centres, Reference laboratories, or organisations in other disciplines, to coordinate scientific and technical studies? Name of OIE CC/RL/other organisation(s) Location Region of networking Centre Purpose Bovine tuberculosis ANSES Laboratoire de Santé Animale Maisons-Alfort, France Africa Americas Asia and Pacific Europe Middle East Exchange of informations for completion of a study on validation of interferon gamma PCR for the diagnostics of bovine tuberculosis ToR: To place expert consultants at the disposal of the OIE. 5. Did your Collaborating Centre place expert consultants at the disposal of the OIE? OIE Collaborating Centres Reports Activities, 2016 3

Name of expert Kind of consultancy Subject Edith Authié Provision of comments on draft revised chapter 2.4.4 of the OIE Terrestrial manual Diagnostic procedures for bovine campylobacteriosis Nathalie Pozzi id id Virginie Catinot id id ToR: To provide, within the designated specialty, scientific and technical training to personnel from OIE Member Countries 6. Did your Collaborating Centre provide scientific and technical training, within the remit of the mandate given by the OIE, to personnel from OIE Member Countries? a) Technical visits: 2 b) Seminars: 0 c) Hands-on training courses: 0 d) Internships (>1 month): 2 Type of technical training provided (a, b, c or d) Content Country of origin of the expert(s) provided with training No. participants from the corresponding country d Development of a Quality assurance system for a cryobank of gametes embryos and somatic cells France 1 d Preparation of culture media for detection of reproductive pathogens France 1 a Assess the capacity of LNCR to run quality controls on semen extenders USA 1 a Visits by delegations from various countries Mongolia, India, China, Sudan 5 ToR: To organise and participate in scientific meetings and other activities on behalf of the OIE 7. Did your Collaborating Centre organise or participate in the organisation of scientific meetings on behalf of the OIE? No 4 OIE Collaborating Centres Reports Activities, 2016

ToR: To collect, process, analyse, publish and disseminate data and information relevant to the designated specialty 8. Publication and dissemination of any information within the remit of the mandate given by the OIE that may be useful to Member Countries of the OIE a) Articles published in peer-reviewed journals: 0 b) International conferences: 1 18th International Congress on Animal Reproduction - Tours (France)- 26-30 June 2016 Chevrier L; Camugli S., Merlet P. Pozzi N. Schmitt E. Flow cytometry assessment of bacterial contamination in artificial insemination doses. c) National conferences: 0 d) Other (Provide website address or link to appropriate information): 0 OIE Collaborating Centres Reports Activities, 2016 5