Macropelopia (Bethbilbeckia) chilensis n. sp. (Diptera, Chironomidae) from Cajón del Maipo, Chile

Norwegian Journal of Entomology. 14 December 2018 Macropelopia (Bethbilbeckia) chilensis n. sp. (Diptera, Chironomidae) from Cajón del Maipo, Chile TROND ANDERSEN Andersen, T. 2018. Macropelopia (Bethbilbeckia) chilensis n. sp. (Diptera, Chironomidae) from Cajón del Maipo, Chile. Norwegian Journal of Entomology 65, 85 90. Macropelopia (Bethbilbeckia) chilensis n. sp. is described and figured based on pharate male pupae and pupa exuviae collected in the Andes Mountains east of Santiago, Chile, in 1998 and 1999. The pupa possesses the large, spine-like D 1 setae arising from prominent tubercles on tergites II VII, characteristic for the subgenus Bethbilbeckia Fittkau & Murray, 1988. However, the thoracic horn is more similar to the thoracic horn in some Macropelopia (s. str.) Thienemann, 1916 species than in the only other species in the subgenus, M. (B.) floridensis Fittkau & Murray, 1988. The male can easily be separated from the male of M. (B.) floridensis by having bi- to multiserial orbitals and a strong comb on the hind leg, characters that are found in Macropelopia (s. str.) but not in M. (B.) floridensis, indicating that the status of Bethbilbeckia as a separate subgenus should be reconsidered. Key words: Diptera, Chironomidae, Tanypodinae, Macropelopia (Bethbilbeckia), new species, Chile, Neotropical region. Trond Andersen, Department of Natural History, University Museum of Bergen, University of Bergen, P.O. Box 7800, NO-5020 Bergen, Norway. E-mail: trond.andersen@uib.no Introduction Fittkau & Murray (1988) erected the genus Bethbilbeckia for B. floridensis Fittkau & Murry, 1988 from Florida, U.S.A. According to Fittkau & Murray (1988), the pupa of Bethbilbeckia is superficially similar to the pupa of Macropelopia Thienemann, 1916, but can easily be distinguished by the prominent, straight D 1 setae that arise from distinct tubercles, larger than those present in any other Macropelopiini. The very short, weak LS setae on segment VIII are also diagnostic for the genus. Fittkau & Murray (1986) had already included the genus as Tanypodinae Genus I in the key to the genera of Holarctic Tanypodinae pupae. In the revised key to the genera of Holarctic Tanypodinae larvae, Cranston & Epler (2013) treated Bethbilbeckia as a subgenus of Macropelopia, stating that it resembles Macropelopia in all life stages. This placement within Macropelopia has later been supported by several phylogenetic studies based both on morphological and molecular data (Krosch et al. 2017, Silva & Ekrem 2016, Siri & Donato 2015). The subgenus Bethbilbeckia is monotypic and the adult male of M. (B.) floridensis differs from all other Macropelopiini, except Fittkauimyia Karunakaran, 1969, in the uniserial arrangement of the temporal (inner and outer verticals and postorbitals) setae. In Macropelopia (s. str.) inner and outer verticals and postorbitals are all multiserial. M. (B.) floridensis also lacks combs on fore- and hind tibiae, characters that are present in Macropelopia. During fieldwork in the Andes Mountains east of Santiago, Chile, in 1998 and 1999, several pharate pupae and pupae exuviae of a species, which possesses the prominent, spine-like D 1 setae arising from distinct tubercles characteristic for Bethbilbeckia, were collected. Most of the 85

Andersen: Macropelopia (Bethbilbeckia) chilensis n. sp. from Chile specimens were collected in a small, shallow, rather rapid stream at an altitude of about 1.800 m a.s.l.. Despite repeated field trips to the area, no adults were collected, so the species is described below as Macropelopia (Bethbilbeckia) chilensis n. sp., based on pharate male pupae and pupae exuviae only. Methods and material Most pupae were collected in driftnets in a small, rapid stream originating in the hot spring Baños Morales a few hundred meters above the collecting site. The stream is situated at about 1.800 m a.s.l. and is 1 2 m wide, rather shallow, with stone and gravel substrate. A single pupa was also collected in a driftnet in a side channel to Rio Volcán at Lo Valdes at about 2.500 m a.s.l.. At the sampling site, the channel was shallow, with stone and gravel substrate. The pupae were conserved in ethanol and later mounted on slides in Canada balsam. Measurements are given as ranges in µm, except for the total length of the pupa, which is given in mm; followed by the mean when more than three specimens were measured; followed by the number of specimens measured, (n), in parenthesis. The morphology terminology follows Sæther (1980). The type material is kept in the entomological collection at the University Museum of Bergen, Bergen, Norway (ZMBN). Macropelopia (Bethbilbeckia) chilensis n. sp. (Figures 1 10). Type material: Holotype: Pharate male pupae, CHILE, Región Metropolitana, San José de Maipo, Cajon del Maipo, Baños Morales, 33.824151 o S 70.062993 o W, 1.835 m a.s.l., 18 19 February 1999, drift net, leg. T. Andersen (ZMBN). Paratypes: 5 pharate male pupae, 3 pharate female pupae, 3 pupae exuviae, as holotype (ZMBN); 1 pharate female pupa, CHILE, Región Metropolitana, San José de Maipo, Cajon del Maipo, Lo Valdes, Rio Volcán, 33.856273 o S 69.982294 o W, 2.540 m a.s.l., 10 November 1998, drift net, leg. T. Andersen (ZMBN). Etymology: Named after the country of origin. Diagnostic characters: The male can easily be separated from the male of M. (B.) floridensis by having bi- to multiserial orbitals and a strong comb on hind tibia; M. (B.) floridensis has uniserial orbitals and lack comb on hind tibia. The pupa possesses the large, spine-like D 1 setae arising from prominent tubercles on tergites II VII, which is characteristic for M. (B.) floridensis. However, the thoracic horn is somewhat triangular and curved and is more similar to the thoracic horn in some other Macropelopia (s. str.) species than in M. (B.) floridensis. Description: Male (pharate male pupae) (n = 5, if not otherwise stated). Coloration. Brown. Antenna. Terminal flagellomere (Figure 1) 96 115, 105 µm long; 27 33, 30 µm wide at base. Head. Eye-bridge 4 ocelli wide. Inner verticals 10 14, 12, multiserial; outer verticals 8 13, 10, bito triserial; postorbitals 11 14, 12, bi- to triserial. Thorax. Dorsocentrals irregularly biserial; acrostichals tri- to multiserial; scutellum with transverse row of 15 22, 18 strong setae and 7 13, 12 weaker setae anterodorsally; postnotum apparently with altogether about 13 (1) setae, biserial; other setae difficult to observe. Scutal tubercle apparently low, difficult to observe in the slides. Wing. Not measurable. Legs. Width at apex of fore tibia 73 77 (3) µm; of mid tibia 70 76, 73 µm; of hid tibia 80 82, 81 µm. Spur of fore tibia 80 86 (3) µm long with 9 14 side teeth (Figure 2); long spur of mid tibia 66 77, 73 µm long with 17 19, 18 side teeth, short spur 57 68, 63 µm long with 11 16, 14 side teeth (Figure 3); long spur of hind tibia 80 89, 86 µm long with 6 9, 8 side teeth, short spur 57 65, 62 µm long with 9 12, 11 side teeth (Figure 4). Fore tibia apparently without comb. Comb of hind tibia with 8 strong setae, longest 69 76, 73 (4) µm long; shortest 48 52, 50 (4) µm long. Claws on all legs long and slender, distally pointed, spinulate in basal 1/3. Hypopygium (Figures 5 6). Tergite IX with 9 13 (3) setae in single to partly biserial posterior row. Gonocoxite 154 160 (3) µm long; gonostylus slender, 139 146 (3) µm long; megaseta 12 14 (3) µm long. 86

Norwegian Journal of Entomology 65, 85 90 (2018) FIGURES 1 6. Macropelopia (Bethbilbeckia) chilensis n. sp., male. 1. Terminal antennal flagellomere. 2. Tibial spur of fore leg. 3. Tibial spurs of mid leg. 4. Tibial spurs and comb of hind leg. 5. Hypopygium, dorsal aspect. 6. Hypopygium, ventral aspect. Pupa (n = 8 10). Total length 5.25 6.98, 6.03 mm. Coloration. Brown. Cephalothorax. Thoracic horn (Figure 8), somewhat triangular, curved; 372 448, 404 µm long; 140 176, 160 µm wide at its widest point; plastron plate 196 256, 214 µm long; external membrane with distinct spines; horn sac not quite filling horn lumen. Thoracic membrane with transverse ridges extending to median suture. Thoracic setae Dc 1 simple, pointed, about 60 µm long; Dc 2 not discernable; Sa simple, pointed, about 160 µm long. Abdomen (Figure 7). Scar on tergite I elongate and pigmented. Shagreen spines (Figure 9) short, blunt, and partially serially arranged in groups of 2 4. Abdominal setae D 1 on tergites II VII large, spine-like and arising from large, prominent tubercles; tergite II: D 1 100 128, 112 µm long, tubercle 88 120, 103 µm long measured along inner margin; tergite III: D 1 116 136, 127 µm long, tubercle 160 188, 174 µm long; tergite IV: D 1 140 160, 151 µm long, tubercle 192 236, 217 µm long; tergite V: D 1 140 160, 151 µm long, 87

Andersen: Macropelopia (Bethbilbeckia) chilensis n. sp. from Chile FIGURES 7 10. Macropelopia (Bethbilbeckia) chilensis n. sp., pupa. 7. Abdominal tergites and anal lobe. 8. Thoracic horn. 9. Shagreen. 10. Anal lobe and male genital sac. 88

Norwegian Journal of Entomology 65, 85 90 (2018) tubercle 212 256, 227 µm long; tergite VI: D 1 140 160, 153 µm long, tubercle 188 220, 199 µm long; tergite VII: D 1 136 164, 153 µm long, tubercle 112 136, 139 µm long. Remaining D and V setae of varying size; D 2 and D 3 on tergite III V arising from small tubercles. Segments I VI with 2 L setae; segment VII with 4 short LS setae; segment VIII with 5 LS setae, longest about the length of the segment. Anal lobe (Figure 10) 458 572, 515 µm wide, 580 669, 641 µm long. With simple spine shagreen laterally. Outer border with fringe of setae-like spinules gradually reduced to indistinct spines at the distal end; inner border divergent, without fringe, but with few, small, indistinct spines preapically in some specimens. Anal macrosetae arise from basal 1/4, about 0.75 times segment length. Larva. Unknown. Discussion The new species is placed in the subgenus Bethbilbeckia due to the prominent, spine-like D 1 setae arising from large tubercles on tergites II VII. Also in most other features, the pupa is very similar to the pupae of M. (B.) floridensis. However, the thoracic horn is somewhat triangular and curved and more similar to the thoracic horn of some Macropelopia (s. str.) species. The male differs from the male of M. (B.) floridensis in several key characters as it has bi- to multiserial orbitals and a strong comb on the hind tibia, characters that are found in Macropelopia (s. str.) but not in M. (B.) floridensis. As no adult males of the new species from Chile were collected, several characters, like the scutal tubercle, are difficult to observe. However, based on the material at hand the species shows a mixture of characters found either in the subgenus Bethbilbeckia or in Macropelopia (s. str.), indicating that the status of Bethbilbeckia as a separate subgenus should be reconsidered. Pupae and larvae of Bethbilbeckia have been recorded from Argentina (García et al. 2007, Krosch et al. 2017). At present, there are no named Macropelopia species from South America, but Ashe & O Connor (2009) list several South American Tanypodinae as unplaced valid. Most of these species were described based on adult material collected in Patagonia and Chile by Edwards (1931). However, the description of none of these species seems to fit the new species from Chile. Acknowledgements. I am indebted to Luiz Carlos Pinho, Universidade Federal de Santa Catarina, Florionópolis, Brazil for comments on the manuscript. References Ashe, P. & O Connor, J.P. 2009. A world catalogue of Chironomidae (Diptera). Part 1. Buchonomyiinae, Chilenomyiinae, Podonominae, Aphroteniinae, Tanypodinae, Usambaromyiinae, Diamesinae, Prodiamesinae and Telmatogetoninae. 445 pp. Irish Biogeographical Society and National Museum of Ireland, Dublin. Cranston, P.S. & Epler, J.H. 2013. 5. The larvae of Tanypodinae (Diptera: Chironomidae) of the Holarctic region Keys and diagnoses. In: Andersen, T., Cranston, P.S. & Epler, J.H. (Eds). Chironomidae of the Holarctic region. Keys and diagnoses Larvae. Insect Systematics & Evolution, Supplement 66, 39 136. Edwards, F.W. 1931. Chironomidae. Diptera of Patagonia and South Chile 2, 233 331. Fittkau, E.J. & Murray, D.A. 1986. 5. The pupae of Tanypodinae (Diptera: Chironomidae) of the Holarctic region Keys and diagnoses. In: Wiederholm, T. (Ed.). Chironomidae of the Holarctic region. Keys and diagnoses. Part 2. Pupae. Entomologica scandinavica, Supplement 28, 31 113. Fittkau, E.J. & Murray, D.A. 1988. Bethbilbeckia floridensis: a new genus and species of Macropelopiini from the South Eastern Nearctic (Diptera: Chironomidae). Spixiana, Supplement 14, 253 259. García, P.E. & Suárez, D.A.A. 2007. Community structure and phenology of chironomids (Insecta: Chironomidae) in a Patagonian Andean stream. Limnologia 37, 109 117. Krosch, M.N., Cranston, P.S., Bryant, L.M., Strutt, F. & McCluen, S.R. 2017. Towards a dated molecular phylogeny of the Tanypodinae (Chironomidae, 89

Andersen: Macropelopia (Bethbilbeckia) chilensis n. sp. from Chile Diptera). Invertebrate Systematics 31, 302 316. Sæther, O.A. 1980. Glossary of Chironomid morphology terminology (Diptera: Chironomidae). Entomologica scandinavica, Supplement 14, 1 51. Silva, F.L.D. & Ekrem, T. 2016. Phylogenetic relationships of nonbiting midges in the subfamily Tanypodinae (Diptera: Chironomidae) inferred from morphology. Systematic Entomology 41, 73 92. Siri, A. & Donato, M. 2015. Phylogenetic analysis of the tribe Macropelopiini (Chironomidae: Tanypodinae): adjusting homoplasies. Zoological Journal of the Linnean Society 174, 74 92. Received: 25 September 2018 Accepted: 29 October 2018 90