Biological Threat Fact Sheets Anthrax Agent: Bacillus anthracis There are three clinical forms of B. anthracis which are determined by route of entry: Pulmonary or Inhalation BT implications Cutaneous most common Gastrointestinal Aerobic or facultative anaerobe Spore-forming Gram-positive rods Non-hemolytic (gamma) Non-motile Cutaneous anthrax: sterile swab from vesicular or eschar stage Gastrointestinal anthrax: Blood culture, stool ( 5 g) or rectal swab Inhalational anthrax: Blood culture, lower respiratory specimen (>1ml)
Brucellosis Agent: Brucella abortus, Brucella melitensis, Brucella suis, and Brucella canis Aerosols of Brucella spp. are considered to be highly infectious. B. melitensis produces the most severe and acute form of disease. B. abortus produces a more chronic form of disease. B. suis may also cause severe acute disease, but is associated with osteomyelitis. B. canis rarely causes disease in humans. Small gram-negative coccobacilli Slow-grower, colonies appear after 48-72 hrs of incubation Non-motile Oxidase positive Urease positive X and V factors not required Blood culture Bone marrow Tissue sample
Botulism Agent: Clostridium botulinum There are three forms of botulism: Foodborne (classic form) Wound Intestinal (infant and adult) Key Points: Please have physician consult with the Infectious Disease Control Unit (IDCU) prior to sample collection: o (800) 252-8239 ( press 1 ) Stool o 10-50 grams recommended for an adult o 5 grams recommended for an infant o Do not freeze Serum o 10ml minimum for an adult is recommended o Not recommended for infant testing Wound o Tissue from a biopsy or swab from deep in the wound Food o Only tested if associated with a confirmed botulism case Shipping conditions: Stools and serum o Should be shipped cold (on cold packs, not dry ice) by overnight courier Wound o Ship tissue in anaerobic atmosphere o Swab in anaerobic transport for swabs o Ship without refrigeration Food o Should be shipped in original container under current storage conditions (Example: cold storage submitted cold; frozen storage submitted frozen; etc)
Tularemia Agent: Francisella tularemia Aerosols of F. tularensis. are considered to be highly infectious. Two subspecies cause human disease: o F. tularensis subsp. tularensis (highly virulent) o F. tularensis subsp. holarctica (less virulent) Tiny gram-negative coccobacilli Slow-grower, colonies appear after 48-72 hrs of incubation Usually requires cysteine supplementation Non-motile Oxidase negative Urease negative Beta-lactamase positive X and V factors not required Blood culture Biopsied tissue or scraping of an ulcer (preferable) Swab of an ulcer (acceptable alternative) Tissue aspirate
Plague Agent: Yersinia pestis Three forms of plague: o Bubonic: infected lymph nodes o Septicemic: blood-borne organisms o Pneumonic: transmissible person-to-person; deadliest Gram-negative coccobacilli with bipolar staining Colonies appear after 24-48 hrs of incubation Grows better at 22-25 C Non-motile Oxidase negative Urease negative Bubonic: Aspirate of involved tissue (bubonic) or biopsied specimen: Tissue or aspirates that can be obtained for culture include liver, spleen, bone marrow, lymph node, and/or lung Septicemic: blood Pneumonic: Bronchial wash or transtracheal aspirate (>1 ml)
Melioidosis Glanders Agent: Burholderia pseudomallei and Burkholderia mallei B. pseudomallei- causative agent of melioidosis B. mallei causative agent of glanders B. pseudomallei o Gram-negative rods with bipolar staining o Oxidase positive o Indole negative o Motile o Resistant to colistin/polymyxin B B. mallei o Faintly staining, gram-negative coccobacilli o Oxidase variable o Indole negative o Non-motile o Resistant to colistin/polymyxin B Blood Urine Abscesses, tissue aspirates, fluids
Ricin Toxin Agent: Ricin toxin Ricin toxin is derived from castor beans, Ricinus communis. Medical tests to confirm that a person has been exposed to ricin are not widely available. No antidote exists for ricin. Clinical: o Texas Department of State Health Services, Austin, does not currently accept clinical samples for ricin toxin testing. o Serum (acute and convalescent 2 weeks apart) testing is performed at the CDC o Not indicated unless a known exposure has occurred Environmental: food, beverage, soil, water, powder, swabs, wipes, paper, plant material o Submitted by law enforcement personnel only Specimen collection: Collect specimens in plastic containers and be triple contained in accordance with federal shipping Store specimens at room temperature or refrigerate NEVER freeze or heat samples suspected of containing ricin toxin, as this will denature the toxin
Staphylococcal Enterotoxin B (SEB) Agent: Staphylococcal enterotoxin B (SEB) SEB is one of several exotoxins produced by Staphylococcus aureus. SEB is the enterotoxin that most commonly causes classic food poisoning. Clinical: Texas Department of State Health Services, Austin, does not currently accept clinical sample for SEB testing. Environmental: paper, powder, swabs, wipes, water, soil, food, beverage o Submitted by law enforcement personnel only
Q Fever Agent: Coxiella burnetii C. burnetii is an obligate intracellular organism and cannot be cultured on routine bacteriologic media. Due to the extreme infectivity of C. burnetii, Level A (Sentinel) laboratories should not attempt to culture this organism. Blood - Collect blood in EDTA (lavender) or sodium citrate (blue) and maintain at 4 C for storage and shipping for PCR or special cultures Please call (512) 458-7185 prior to submission.