Indiana: Ready for Anything

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March 206 The goal of the American Sheep Industry Association and the U.S. sheep industry is to eradicate scrapie from our borders by 207. In addition, it is the objective to have the United States recognized as scrapie free in accordance with the World Organization for Animal Health. This quarterly publication is created specifically for those of you in the field who are also working to achieve this goal. This newsletter brings together, into one spot, current information from all 50 states, as well as from the U.S. Department of Agriculture and any other organization providing scrapie news, and reports it back to the field. If you have first-hand accounts that you believe would be relevant for others to read or have information that you would like included in this newsletter, please email judym@sheepusa.org. Indiana: Ready for Anything BY CHERYL MLER, DVM As regulatory veterinarians, we would prefer to deal with herd/ flock health challenges one at a time. Unfortunately, that is not what usually occurs, as we experienced earlier this year, here in Indiana. In the midst of investigating bovine tuberculosis, exposed dairy cattle moved into a large dairy and responding to an outbreak of avian influenza involving 0 sites, I received notice of our first scrapie positive flock in Indiana since 202. I m sure we all had that deer in the headlights look as we feared what was ahead of us. The scrapie-positive flock owner was informed of the test results and his flock was quarantined. The original positive ewe was a 3-year-old that was confirmed as AAQQ by the National Veterinary Services Laboratory. Fortunately, the producer was very cooperative and wanted to get his flock cleaned up as soon as possible. The flock was a black-faced, club-lamb flock consisting of approximately 70 adult sheep. They were in the middle of lambing and still had several ewes to lamb. We codon tested 66 ewes, seven rams, and 69 sexually intact lambs. Out of this group, we identified 9 QQ sheep, including a pregnant daughter of the original positive ewe. This ewe was removed from the flock prior to lambing, and was diagnosed as scrapie positive. Seventeen additional QQs were removed and one additional positive was identified (One of the 9 QQs was a ram lamb which the owner castrated). Cleaning and disinfecting has been completed and verified. The lambs born after the initial round of genotyping are currently being genotyped, once the testing is completed we will remove any remaining QQ ewe lambs. Fortunately, the Indiana Board of Animal Health did not have many traces. Illinois, Iowa and Michigan each received one ewe lamb trace. Ohio received three ewe lambs. Indiana had one ewe lamb trace to another producer which was confirmed as QR. Cull ewes and some lambs were sold through a local sale barn and livestock dealers. BOAH is still working on these traces. As both a field veterinarian and the designated scrapie epidemiologist for Indiana, I was actively involved in all three events. Fortunately, no additional positive sites were found for avian influenza. I am very thankful that my co-workers were willing to jump in and work until the job was done and the producers were very cooperative also. Hopefully, Indiana will not face a triple threat again anytime soon. Submitting Mature Heads APS provides shipping boxes and labels for the submission of heads for scrapie testing at no cost to producers. Many veterinary diagnostic laboratories also accept heads for scrapie testing. To request a box or more information on sample submission, contact the veterinary services field office for your state. State contact information is available at www.aphis.usda.gov/animal_health/area_offices (select contact us from the side bar). VS field offices can also be reached through the toll free number, 866-873-2824. Free For Producers The National Scrapie Eradication Program provides scrapie program official free of charge to producers (call 866-UA-TAG to get tags), testing of exposed flocks and indemnity for infected flocks. Contact your State or local VS office for more information. To view the National Scrapie Eradication Program current Monthly Report, prepared by the U.S. Department of Agriculture s Animal and Plant Health Inspection Service s Veterinary Services, go to https://www.aphis.usda.gov/animal_health/animal_diseases/scrapie/downloads/monthly_scrapie_report.pdf

Animals Sampled for Scrapie Testing Sheep and Goats In Fiscal Year 206, as of Feb. 29, 206 4,434 animals have been sampled for scrapie testing: 3,768 RSSS and 666 on-farm samples,268 sheep and 3,66 goats. 2 sheep and 0 goats have tested positive Regulatory Scrapie Slaughter Surveillance (RSSS) Statistics Since April, 2003 In FY 206 (as of Feb. 29, 206) 496,370 samples collected 3,768 samples collected (2,99 from goats) 479 SL* confirmed positives SL confirmed positives *National Veterinary Services Laboratories On-Farm Surveillance In Fiscal Year 206, as of Feb. 29, 206 666 animals have been tested on farm 49 sheep and 247 goats Infected and Source Flocks New Statuses by Year - Fiscal Years 997 to 206* 200 50 00 Infected Source 50 0 (Chart 2) * As of February 29, 206.

Percent of RSSS Sheep Samples that Tested Positive for Classical Scrapie - Weighted by Face Color 0.6% 0.4% 0.2% 0.0% 0.08% 0.06% 0.04% 0.02% 0.00% Fiscal Years 2003 to 206* % Pos '03 % Pos '04 % Pos '05 % Pos '06 % Pos '07 % Pos '08 % Pos '09 % Pos '0 % Pos ' % Pos '2 % Pos '3 % Pos '4 % Pos '5 % Pos '6 * As of February 29, 206. Adjusted to exclude multiple positive animals from the same flock. Does not include Nor98-like scrapie cases found through RSSS (2 in FY 2007, in FY 2008, 4 in FY 200, in FY 20). 6000 5000 Slaughter Surveillance Samples Collected by Month Fiscal Years 202 to 206 As of February 29, 206 4000 3000 2000 000 FY 2 FY 3 FY 4 FY 5 FY 6 0 0* 2 2 3 4 5 6 7 8 9 Note: Surveillance numbers were significantly lower October 203 compared to October in prior FYs because the furlough reduced the number of collection days. (Chart 5)

FY 206 Scrapie Confirmed Cases by State As of February 29, 206 State Sheep Goats RSSS On-Farm RSSS On-Farm IN 2 0 0 0 0 0 OH 0 8 0 0 Total All States 0 0 Scrapie Infected and Source Flocks Open Statuses - As of February 29, 206 IN OH VA Open Source 3 Open Infected

New Scrapie Infected and Source Flocks - FY 206 As of February 29, 206 IN OH 2 VA New Source 3 New Infected State Sampling Minimums The National Scrapie Eradication Program establishes annual sheep and goat sampling minimums for each state, and tracks the states level of compliance with meeting these minimums. These state minimums help ensure adequate geographical representation, so that APS can find the last remaining cases and document freedom from scrapie. State sampling minimums are established based on the population demographics of mature sheep in each state. The calculations used to derive the sampling minimums are described in the National Scrapie Surveillance Plan. Progress toward meeting these minimums in FY 206 is shown in the following two slides. Percent of Sampling Minimum Achieved in FY 206 RSSS and On-farm Surveillance Sheep* 20% 2-40% IN OH VA MD 4-60% 6-80% 8-99% 00% + * As of February 29, 206. Percentage of sampling minimum achieved is based on 35% of the annual sampling minimum.

Percent of Sampling Minimum Achieved in FY 206 RSSS and On-farm Surveillance Goats* 20% 2-40% IN OH VA MD 4-60% 6-80% 8-99% 00% + * As of February 29, 206. Percentage of sampling minimum achieved is based on 40% of the annual sampling minimum., and have a sampling minimum of. and MD had a large increase in their minimums due to finding an infected herd in each State in FY 204. Websites Dedicated to the Eradication of Scrapie Animal and Plant Health Inspection Service: www.aphis.usda.gov/animal_health/animal_diseases/scrapie Maryland Small Ruminant Page: www.sheepandgoat.com/scrapie.html National Institute of Animal Agriculture: www.animalagriculture.org/scrapie/scrapie.htm Scrapie SharePoint: http://animalhealth/scrapie/default.aspx (Federal employees can access this password-protected site by emailing Diane.L.Sutton@aphis.usda.gov if you need assistance. State employees can request a copy on CD.)