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Indicative Budget for implementation of the Bovine Brucellosis Control Programme in 2016 1. Introduction ANNEX 6.1 Given the current epidemiological situation of brucellosis in cattle in Albania, a flexible, responsive, stepwise approach toward its control is adopted, which is uniquely suited to the conditions in Albania. Details of the measures and their timeline for implementation are presented in the Bovine Brucellosis Control Programme (BBCP) as adopted by the Veterinary Directorate of the Ministry of Agriculture, Rural Development and Water Administration (MARDWA). Financial resources for implementation of the BBCP are secured from the State budget of the Veterinary Directorate. The implementation of the BBCP is also by supported by PAZA II project which cover costs for diagnostic reagents and consumables including training for the official and private veterinarians, raising awareness, specimen submission, etc. Although with the given lack of supporting information especially of the disease presence it is not possible to prepare much precise calculation, an indicative budget for implementation of the BBCP based on numerous assumptions indicates the costs that will be covered from the state budget of the Veterinary Directorate MARDWA. Provided calculations does not attempt to simulate the real disease situation in Albania but rather to assess the financial resources required for implementation of the control programme in 2016. An official decision on the unit prices for field activities and laboratory tests is required to finalize the indicative budget for 2016. 2. Materials and Methods 2.1. Materials Comprehensive and reliable calculation of a disease control options requires relevant and reliable data, arising from the field observations and scientific data available. Given that very limited relevant and reliable data is readily available, as much as possible realistic assumptions based on scientific references is adopted that are driving the calculation of the budget for implementation. The current calculation of the costs for implementation of the Bovine Brucellosis Control Programme are based on the assumptions presented in the Tables below. Assumptions used for calculations of the indicative budget were adopted during several consultative meetings with representatives of the Veterinary Directorate. The unit prices that were used for the calculation of the costs are not official and are only presenting assumptions to the foreseen adopted prices.

Table 1: National categorization of cattle herds in Albania based on herd sizes National categorization of cattle herds in Albania, based on herd sizes Category Number of cattle in holding Number of holdings number of cattle per holding Total number of cattle in the category Percentage of cattle population per category I >50 II 21 to 50 III 11 to 20 IV 5 to 10 V 1 to 4 Source: Farm register 2011 198 532 1,333 7,390 166,035 Total number of cattle in registered holdings 96 32 15 6 2 18,971 17,228 19,481 46,634 272,903 375,217 Table 2: Assumed unit prices for implementation of the Bovine Brucellosis Control Programme in 2016 Parameter Unit price Comment in ALL milk sample 1,000 In 2016 it is foreseen that milk samples will be collected by OVs who will be paid a lump sum for transportation to the farm blood samples 50 In 2016 blood samples will be collected by contracted private veterinarians from the holdings where positive result on MRT were obtained Laboratory cost for MRT 900 Price was adopted based on calculations of the costs for performance of the single test. In 2016, reagents for MRT were supplied by PAZA II project Laboratory cost for RBPT 45 Price was adopted based on calculations of the costs for performance of the single test. In 2016, reagents for RBPT were supplied by PAZA II project Laboratory cost for CFT 272 Calculations foresees use of ELISA test as a confirmatory test Laboratory cost for ELISA 677 Price was adopted based on calculations of the costs for performance of the single test. In 2016, reagents for MRT were supplied by PAZA II project. Laboratory cost for 500 Not confirmed price by AHD ISUV bacteriology Disinfection costs 80 It is not official price. In addition, responsible person for disinfection has to be appointed. 5 5 5 12 73 100

Compensation for the positive cattle will be done based on the procedure adopted with the Ministerial Order No.??. Given that wide range of cattle species will be slaughtered, a range of expected unit prices were adopted to accommodate for the expected uncertainties. Table 3: Compensation for positive cattle Parameter Unit price Comment Compensation costs (min) 70,000 The range was adopted given the previous Compensation costs 100,000 experience with compensation of positive (mean) cattle Compensation costs (max) 120,000 Given that mainly larger farms are subject of the BBCP in 2016, relatively large surfaces are assumed. Table 4: Assumed surfaces for disinfection in positive holdings Parameter Unit price Comment Disinfection surface (min) 1,000 m 2 An average of 1,500 m 2 is adopted given that Disinfection surface (mean) 1,500 m 2 in case of positive animals complete cleansing Disinfection surface (max) 2,000 m 2 and disinfection is foreseen Figures below assumes the range of expected herd and individual animal which greatly influence the financial resources required for implementation of the BBCP. In the absence of solid and reliable information, below indications are only opinion based on previous experience and scientific knowledge of an endemic situation within any country. Much more reliable data will be available after the start of the implementation of the BBCP when budget may be further fine-tuned. Table 5: and within infected herd individual animal per category Category I Category II (MRT) Assumed individual animal (MRT) Assumed individual animal Min 1.00% 1.00% 5.00% 1.00% 1.00% 5.00% 5.00% 2.50% 12.00% 6.00% 4.00% 12.00% Max 10.00% 5.00% 20.00% 12.00% 6.00% 20.00%

2.2. Method A spreadsheet using RiskAMP Add-in for Excel was constructed with available information and adopted assumptions to simulate the probability distribution of anticipated costs for implementation of BBCP for each holding category. Simulation for each category was conducted using Monte Carlo procedure. One thousand iterations were conducted to establish the minimal and maximum expected costs. In addition, out of 1,000 iterations, a mean value was derived. 3. Calculation of costs With the given assumptions and unit values, expected range of positive herds and animals for each category was calculated (Table 6). Table 6: herds and positive animals after implementation of the foreseen measures herds (MRT) Category I herds animals Min 2 2 15 11 6 66 Max 20 10 167 Category II herds (MRT) herds animals Min 6 6 17 34 19 76 Max 63 31 170 Total Category I & II herds (MRT) herds animals Min 8 8 33 44 25 142 Max 82 41 337 Expected output in the number of animals that shall be subjected to individual animal tests is presented in Table 7.

Table 7: Expected number of animals to be subjected to individual animal tests Category I Category II Total Category I & II Animals to be covered with individual tests (RBPT&ELISA or CFT) Min 144 301 445 483 1,004 1,487 Max 909 1,961 2,870 Based on the above assumptions, detailed costs were calculated as follows. Costs for screening for the disease includes collection of samples and laboratory testing and are presented in Table 8 below. Table 8: Costs for screening activities including sampling and laboratory investigation specimens for milk ring tests (three tests annually) Screening costs in Albanian Lek in holdings of Category I Confirmation of Confirmation Milk ring testlaboratory samples for blood diseaselaboratory tests laboratory of disease- costs confirmation tests (ELISA) of disease Bacteriology Min 594,000 534,600 21,570 19,413 404,394 7,697 594,000 534,600 72,503 65,253 1,086,583 32,769 Max 594,000 534,600 136,348 122,713 1,886,235 83,370 specimens for milk ring tests (three tests annually) Screening costs in Albanian Lek in holdings of Category II Milk ring testlaboratory costs blood samples for confirmation of disease Confirmation of diseaselaboratory tests Confirmation of diseaselaboratory tests (ELISA) Bacteriology Min 1,436,400 45,166 40,649 394,302 8,603 1,436,400 1,436,400 150,590 135,531 1,259,584 38,042 1,436,400 Max 1,436,400 294,139 264,725 2,036,526 85,145 1,436,400 specimens for milk ring tests (three tests annually) Screening costs in Albanian Lek in holdings of Category I & II Milk ring testlaboratory costs blood samples for confirmation of disease Confirmation of diseaselaboratory tests Confirmation of diseaselaboratory tests (ELISA) Bacteriology Min 2,190,000 1,971,000 66,736 60,062 798,697 16,301 2,190,000 1,971,000 223,093 200,784 2,346,167 70,811 Max 2,190,000 1,971,000 430,488 387,439 3,922,761 168,516

Compensation and disinfection costs according to the assumed range of herd and individual animal are presented in Table 9 and Table 10 below. Table 9: Compensation costs Compensation costs in Albanian Lek Category I Category II Total Category I & II Min 1,605,065 1,570,595 3,175,660 6,344,872 7,404,057 13,748,929 Max 16,771,215 17,377,025 34,148,241 Table 10: Disinfection costs Disinfection costs in Albanian Lek Category I Category II Total Category I & II Min 202,939 623,063 826,003 6,344,872 2,318,332 8,663,204 Max 16,771,215 4,696,143 21,467,359 The summary of all costs for implementation of the activities that are foreseen to be covered from the national budget with the given assumptions are given in the Table xx. Table 11: Summary of costs for implementation of activities as foreseen with the BBCP in 2016 Summary Costs in Albanian Lek Min Max milk Collection of blood for MRT for RBPT for ELISA bacteriology Compensation Disinfection TOTAL 2,190,000 66,736 1,971,000 60,062 798,697 16,301 3,175,660 826,003 9,104,462 2,190,000 223,093 1,971,000 200,784 2,346,167 70,811 8,663,204 8,663,204 24,328,265 2,190,000 430,488 1,971,000 387,439 3,922,761 168,516 34,148,241 21,467,359 64,685,805