Trials to control Western Corn Rootworn (Diabrotica virgifera virgifera) in Austria

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Trials to control Western Corn Rootworn (Diabrotica virgifera virgifera) in Austria Rosner J.: Ofiice of the Lower Austrian Provincial Government, Department of Agricultural Education, Frauentorgasse 72, A- 3430 Tulln Austria Foltin, K.: Agro ds Austria, Wulkaprodersdorf, Austria

Hansag Seewinkel Burgenland 2008

Aging - feeding on the ears with reduced fertilization by eating on the silks

Strategies for monoculture (continuous corn) Seed treatment or seedrillaplication, band, T-band, furrow application with granules or liquids treatment of young plants with granules or liquids into the root zone (Talstar active substance Bifentrin) Cornplanter with seedrill (furrow) application granule drill for Bifenthrin Spraying the beetles

Test variants 2005-2006 Variant 1.1. und 1.2.: Wide angle nozzle - 2005 Variant 2: Air assisted sprayer Variant3: Conventonal Spraying with high boom / Teejetnozzles Variant 4: Drag hoses with double nozzles 2006 2 double nozzles on the high boom Spray wide 8 m boom width 15 m Boom width 15 m Boom width 15 m Spray pressure 3 bar Spray pressure 3 bar Spray pressure 3 bar Spray pressure 3 bar application: 10.August 2005; 4.und 17. August 2006, spray water rate 400l/ha Diabrotica Pheromone trap PAL Deutsch Jahrndorf beginning of August 2005 before insicticide spraying

Catch result 2005 1.screening control untreated Insecticide efficacy % Control untreated Insecticide efficacy % Spray technologyvariant 2 days after spraying 12.08.2005. 2 weeks after spraying and change of the trap 17.08.2005 Variant 1: : wide angle nozzle No datas 360 513 410 20 % Variante : air assited sprayer 225 70 69 % 190 110 42 % Variante : conventional spraying with high boom and tee jet nozzles 1050 310 70 % 300 180 40 % Variante :drag hoses with double nozzles on the high boom 365 220 40 % 230 140 40 %

Untreated cotrol 2005 Insecticide spraying

Results exact trial 2006 100 % Wirkung % efficacy % efficacy days after - 1 Application 2006 90 80 70 60 50 Luftuntetrstützt air assited sprayer 1 Applikation Konventionell conventional sprayer 1 Applikation Schleppschläuche drag hoses 1 Applikation 40 30 20 10 0 1.5 6 15 19 days after 35spraying Tage nach Applikation

Results exact trial 2006 100 % efficacy % efficacy days after - 2 Applications 2006 2. Application 90 80 70 60 50 40 30 20 Luftunterstützt air assited sprayer 2 Applikationen Konventionell conventional 2 sprayer Applikationen Schleppschläuche drag hoses 2 Applikationen 10 0 2 6 2 6 22 days after spraying

Untreated control Air assisted sprayer Pheromone traps 6 days after spraying Conventional spraying with high boom Drag hoses with double nozzles

results water sensitive paper test 2005-2006 The improved coverage was rated optic with a 1 9 scale 90% improved coverage = 1 80% improved coverage = 2 70% improved coverage = 3.. 10% improved coverage = 9 Spray technlogy - variant Improved coverage Scale 1-9 2005 2006 Variant 1: : wide angle nozzle 8 9 - Variant 2 : air assited sprayer 1 3 1-2 Variante 3 : conventional spraying with high boom and tee jet nozzles 3 5 4-6 Variante 4 :drag hoses with one nozzle on the high boom 3 4 Variant 4.1.Drag hoses with double nozzles on the high boom 2-3

Result Wate sensitive Paper Test: The improved coverage was rated optic with a 1 9 scale 90% improved coverage = 1, 80% improved coverage = 2, 70% improved coverage = 3.. 10% improved coverage = 9 Air assisted sprayer Drag hoses with 1 double nozzle on the high boom Air assited sprayer Conventional spraying with high boom Drag hoses with 2 double nozzles on the high boom a.50 cm over cornear b. Height corn ear c. 50 cm under corn ear

Drag hoses with 1 double nozzle on the high boom Risk of wind drift 50 cm over ear Hight ear Improved coverage: 50 cm unde ear 50 cm over ear Hight ear 50 cm under ear 2 3 3 2 2 3 2 2 2 50 cm over ear Hight ear 50 cm under

Drag hoses with unaccaptable spray coating High boom with drag hoses and double nozzles 2005

Drag hoses with 2 double nozzles 2006 Dangle of the drag hoses in the corn

Air assited spray technology High boom in the corn

High clearance tractor Van sprayer with high risk of wind drift

Diabrotica control with Seed treatment or seedrillaplication, band, T-band, furrow application with granules treatment of young plants with granules into the root zone Hansag Wallern Seewinkel/Burgenland 2008-11

Diabrotica larva -control Seewinkel 2008 Test - variants: Active substance Application rate 1. Control untreated 2. Poncho Pro seedtreatment Clothianidin 104 ml/unit (50.000 grains) 3. Fipronil Granule drill Fipronil 5 kg/ha 4. Force 1,5 G Granule drill Tefluthrin 15g/kg 13,3 kg/ha - 0.95 g/m 5. Fipronil Granule young plant larva control Fipronil 5 kg/ha 6. Force 1,5 G Granule young plant larva control Tefluthrin 15g/kg 13,3 kg/ha 7. Poncho Pro + Fipronil Granule young plant larva control Clothianidin+Fipronil 104ml+5kg 8. Poncho Pro + Force Granule young plant larva control Clothianidin + Tefluthrin 104ml+13,3kg 9.( Poncho Pro + Fipronil Granule drill) + Fipronil Granule young plant larva control (Clothianidin + Fipronil) + Fipronil (104ml+5kg)+5kg 10. ( Poncho Pro + Force Granule drill) + Force Granule young plant larva control (Clothianidin + Tefluthrin) + Tefluthrin (104ml+13,3kg)+13,3kg

root zone application implement

Blade tines for granuledrill larva control

Diabrotica larva Control2008 10. ( Poncho Pro + Force Granule drill) + Force Granule young plant larva control 2,3 9.( Poncho Pro + Fipronil Granule drill) + Fipronil Granule young plant larva control 8. Poncho Pro + Force Granule young plant larva control 2,2 2,5 IOWA Scale 1 6 7. Poncho Pro + Fipronil Granule young plant larva control 2,5 6. Force 1,5 G Granule young plant larva control 2,4 5. Fipronil Granule young plant larva control 2,1 4. Force 1,5 G Granule drill 2,3 3. Fipronil Granule drill 2. Poncho Pro seedtreatment 2,5 2,5 1. Control untreated 2,9 0,0 1,0 2,0 3,0 4,0 5,0 6,0 IOWA - Scale 1-6 Damage threshold

Goose neck % plants 2008 10. ( Poncho Pro + Force Granule drill) + Force Granule young plant larva control 0,3 9.( Poncho Pro + Fipronil Granule drill) + Fipronil Granule young plant larva control 0,8 8. Poncho Pro + Force Granule young plant larva control 1,0 7. Poncho Pro + Fipronil Granule young plant larva control 0,5 6. Force 1,5 G Granule young plant larva control 1,0 5. Fipronil Granule young plant larva control 2,0 4. Force 1,5 G Granule drill 0,5 3. Fipronil Granule drill 1,8 2. Poncho Pro seedtreatment 1,0 1. Control untreated 3,5 0,0 0,5 1,0 1,5 2,0 2,5 3,0 3,5 goose neck % plants

below ear flatten plants 2008 10. ( Poncho Pro + Force Granule drill) + Force Granule young plant larva control 0,0 9.( Poncho Pro + Fipronil Granule drill) + Fipronil Granule young plant larva control 0,2 8. Poncho Pro + Force Granule young plant larva control 7. Poncho Pro + Fipronil Granule young plant larva control 0,0 0,0 6. Force 1,5 G Granule young plant larva control 0,8 5. Fipronil Granule young plant larva control 0,2 4. Force 1,5 G Granule drill 0,3 3. Fipronil Granule drill 2. Poncho Pro seedtreatment 0,5 0,5 1. Control untreated 1,5 0,0 0,2 0,4 0,6 0,8 1,0 1,2 1,4 1,6 % flatten plants

Diabrotica larva - control Seewinkel 2009 Test - variants: Active substance Application rate 1. Control untreated 2. Poncho seedtreatment 3. Poncho Pro seedtreatment 4. Cruiser 350 FS seedtreatment 5. Force 1,5 G Granule drill 6. Poncho Pro Seedtreatment + Force 1,5 G Granule drill 7. Cruiser 350 FS Seedtreatment + Force 1,5 G Granule drill 8. Force 1,5 G Granule young plant larva control 9. Poncho Pro + Force 1,5 G Granule young plant larva control 10. Cruiser 350 FS seedtreatment + Force Granule young plant larva control 11.Force 1,5 G Granule drill + Force 1,5 G Granule young plant larva control 12. ( Cruiser 350 FS seedtreatment + Force Granule drill) + Force Granule young plant larva control 13. (Poncho Pro seedtreatment + Force 1,5 G Granule drill) + Force Granule young plant - larva control Clothianidin 600 g/l 41,87 ml/unit (50.000 grains) Clothianidin 600 g/l 104 ml/unit Thiametoxam 350 g/l 90 m/unit Tefluthrin 15g/kg 13,3 kg/ha - 0.95 g/m

Blade tines for granuledrill larva control

root damage IOWA 1-6 scale 2009 13. (Poncho Pro seedtreatment + Force 1,5 G Granule drill) + Force Granule young plant - larva control 12. ( Cruiser 350 FS seedtreatment + Force Granule drill) + Force Granule young plant larva control 11.Force 1,5 G Granule drill + Force 1,5 G Granule young plant larva control 10. Cruiser 350 FS seedtreatment + Force Granule young plant larva control 9. Poncho Pro + Force 1,5 G Granule young plant larva control 8. Force 1,5 G Granule young plant larva control 7. Cruiser 350 FS Seedtreatment + Force 1,5 G Granule drill 6. Poncho Pro Seedtreatment + Force 1,5 G Granule drill 5. Force 1,5 G Granule drill 4. Cruiser 350 FS seedtreatment 3. Poncho Pro seedtreatment 2. Poncho seedtreatment 1. Control untreated 2,32 2,25 2,45 2,47 2,5 2,37 2,4 2,57 2,5 2,42 2,3 2,35 2,45 IOWA 4 5 0 1 2 3 4 5 6 Damage threshold

Root damage IOWA 1-6 scale 2010 Wurzelbonitur IOWA Scale 1-6 19. Juli 2010 Larva control Granule drill Goldor Bait - Jungplanzenbehandlung Goldor Bait - Saatrille Granulat NAK 4455 Transfluthrin 3,55 3,47 3,62 Grannulat YRC 2894 3,97 V-Beizung 3-2010 V-Beizung 2-2010 3,7 3,7 V-Beizung 1-2010 Poncho Pro + Force Saatrille + Force Jungpflanzenbeh. 3,92 3,9 (Cruiser 350 FS + Force 1,5 G Saatrille) + Force 1,5 G Jungpflanzen - Larvenbekämpfung 3,05 Combined Force 1,5 G Saatrille + Force 1,5 G Jungpflnazen - Larvenbehandlung seedtreatment + larva control Cruiser 350 FS + Force 1,5 G Jungpflanzen - Larvenbehandlung Young plant larva control Poncho Pro + Force Jungpflanzenbeh. Force Jungpflanzenbehandlung Cruiser 350 FS + Force 1,5 G Saatrille 3,27 3,37 3,37 3,62 3,72 Seadtreatment+Granule drill Poncho Pro + Force 1,5 G Saatrille Force Saatrille 3,42 3,55 Cruiser 350 FS 3,98 seedtreatment Poncho Pro 3,3 Poncho 3,47 Kontrolle 3,72 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 Damage threshold IOWA 1-6

Diabroticabekämpfungsversuch Wallern 2010 Goldor Bait - Larvenbekämpfung 71 Goldor Bait bei Aussaat 91 V - Granulat 2 NAK 4455 103 V - Granulat 1 Y RC 2 8 9 4 100 V - B eizung 3 100 V - B eizung 2 104 V - B eizung 1 98 Poncho Pro + Force Saatrille + Force Jungpflanzenbeh. 89 ( Cruiser 350 FS + Force 1,5 G Saatrille) + Force 1,5 G Jungpflanzen - Larvenbekämpfung 94 Force Saatrille + Force Jungpflanzenbehandlung 89 Cruiser 350 FS + Force 1,5 G Jungpflanzen - Larvenbehandlung 89 Combined treatments + larva control Poncho Pro + Force Jungpflanzenbeh. Force Jungpflanzenbehandlung 84 85 Seedtreatment + Granule drill Granule drill seedtreatment Cruiser 350 FS + Force 1,5 G Saatrille Poncho Pro + Force Saatrille Force Saatrille Cruiser 350 FS Po ncho Pro Po ncho Ko nt ro lle 92 91 94 96 96 102 100 50 60 70 80 90 100 110 Ertrag in % Kontrolle (100%) GD 5 %...13 % Yield in % control (100%) LSD 5%...13 % from control

Wurzelbonituren IOWA Scale 1-6 mehrjährig Rootdamage IOWA scale 1 6 several years Cruiser 350 FS + Force 1,5 G Saatrille 2,9 Poncho Pro + Force 1,5 G Saatrille 3 Granule drill Force 1,5 G Saatrille 3,1 Cruiser 350 FS 3,2 Poncho Pro Poncho 2,8 3 seedtreatment Kontrolle unbehandelt Untreated control 3,1 0 1 2 3 4 5 6 Damage threshold IOWA 1-6

Wurzelbonituren IOWA Scale 1-6 mehrjährig Rootdamage IOWA scale 1 6 several years Saatgut unbehandelt + Goldor Bait - Jungpflanzen - Larvenbehandlung Goldor Bait Saatrille 2,9 3 Larva control Granule drill ( Poncho Pro + Force 1,5 G Saatrille) + Force 1,5 G Jungpflanzen - Larvenbekämpfung (Cruiser 350 FS + Force 1,5 G Saatrille) + Force 1,5 G Jungpflanzen - Larvenbekämpfung Force 1,5 G Saatrille + Force 1,5 G Jungpflanzen - Larvenbehandlung 2,7 2,9 3,1 Larva control Cruiser 350 FS + Force 1,5 G Jungpflanzen - Larvenbehandlung 3,1 Poncho pro + Force 1,5 G Jungpflanzen - Larvenbehandlung 3 Force 1,5 G Jungpflanzen - Larvenbekämpfung 3,1 Kontrolle unbehandelt Untreated control 3,1 IOWA 1-6 0 1 2 3 4 5 6 Damage threshold

Untreated control

Diabroticabekämpfungsversuch Wallern Ertrag 2008-2010 Yield in % unteated control 2008-2010 V - Beizung 2 104 V - Beizung 1 Poncho Pro + Force Saatrille + Force Jungpflanzenbeh. (Cruiser 350 FS + Force 1,5 G Saatrille) + Force 1,5 G Jungpflanzen - Larvenbekämpfung Force Saatrille + Force Jungpflanzenbehandlung Cruiser 350 FS + Force 1,5 G Jungpflanzen - Larvenbehandlung Poncho Pro + Force Jungpflanzenbeh. 92 92 94 97 98 97 Larva control Force Jungpflanzenbehandlung 90 Cruiser 350 FS + Force 1,5 G Saatrille 100 Poncho Pro + Force Saatrille 102 Force Saatrille Cruiser 350 FS 99 98 Poncho Pro 100 P oncho 97 Kontrolle 100 50 60 70 80 90 100 110 Ertrag in % Kontrolle (100%)

Ertrag Diabroticaversuch % vom Standard mehrjährig % vom Standard 2010 Cruiser 350 FS + Force 1,5 G Saatrille 94 100 Poncho Pro + Force Saatrille 102 102 Force Saatrille 96 99 Cruiser 350 FS 92 98 Poncho Pro 96 100 Poncho 91 97 Kontrolle 100 100 Yield 2008-2010 Yield 2010 50 70 90 110 Ertrag in % Kontrolle GD 5 % 2010.13 %

Ertrag Diabroticaversuch % vom Standard mehrjährig % vom Standard 2010 Goldor Bait - Larvenbekämpfung 71 Wurzelverletzungen bei Unterfußapplikation Goldor Bait bei Aussaat 91 Poncho Pro + Force Saatrille + Force Jungpflanzenbeh. 89 98 (Cruiser 350 FS + Force 1,5 G Saatrille) + Force 1,5 G Jungpflanzen - Larvenbekämpfung 94 94 Force Saatrille + Force Jungpflanzenbehandlung 89 92 Cruiser 350 FS + Force 1,5 G Jungpflanzen - Larvenbehandlung 89 92 Poncho Pro + Force Jungpflanzenbeh. 84 97 Force Jungpflanzenbehandlung 85 90 Kontrolle 100 100 Yield 2008-10 Yield 2010 50 60 70 80 90 100 Ertrag in % Kontrolle

Larva control caused a growth reduction (depression) of 10 20 % until harvest + chlorosis leaf yellowing 10 % root damage by tools (blade tines for granule drill under roots) possible

crop rotation Conclusion 2-3 years corn 1 year other plants The insecticides against the adults are limited in their mode of action and to repeat Under wet conditions good replacement of rootdamages by corn