COURSE ON LORTORY NIML SCIENCE VII 13-24 pril 2015 Coordination: Marie Kamber, DVM, PhD Tel: +39 06 90091 223 Fax: +39 06 90091 272 Web: www.embl.it Email: maria.kamber@embl.it
WEEK 1 Time Lectures / practical / Groups Monday 13 pril 2015 09.00-09.15 Opening Session P.vner 09.15-10.15 FELS Courses and new EU Directive Jan as Prins 10.15-11.15 Introduction Lab. nimal Science 11.15-11.30 11.30-13.15 Ethics of animal experimentation: video and discussion 13.15-14.15 Lunch break 14.15-16.15 Welfare and experimental procedures 16.15-17.15 Italian legislation G.Perretta 17.15-18.15 Preparation protocol (introduction) M.Kamber,T.Paparountas Tuesday 14 pril 2015 09.00-10.00 Nutrition Marcus rielmeier 10.00-10.45 Design of animal facilities Rainer Post 10.45-11.45 Practical training (handling) intro + M.Kamber, C. Johner.Giannakopoulou, V.alafas, M.D Ercole, V.Paribeni 12.00-13.00 Lunch break 13.00-15.15 Preparation protocol, Self study T.Paparountas Practical handling & restraining tech. 15.15-15.30 M.Kamber, C. Johner.Giannakopoulou, V.alafas,M.D Ercole,V.Paribeni 15.30-17.45 Preparation protocol, Self study T.Paparountas Practical handling & restraining tech. M.Kamber, C. Johner.Giannakopoulou, V.alafas,M.D Ercole, V.Paribeni 17.45-18.30 Cryopreservation M. Raspa 15 pril 2015 09.00-10.00 Pre lecture & demonstration of experimental techniques: adm. of substances, suturing 10.00-10.15 10.15-12.45 Preparation protocol, Self study T.Paparountas Practical experimental techniques: adm. of substances; suturing 12.45-13.45 Lunch break, M.Kamber, C. Johner V.alafas, S.Tatti,.Giannakopoulou 13.45-16.15 Preparation protocol, Self study T.Paparountas Practical experimental techniques: adm. of substances; suturing, M.Kamber, C. Johner V.alafas,S.Tatti.Giannakopoulou
WEEK 1 Time Lectures / practical / 15 pril 2015 16.15-16.30 16.30- Guidelines for a safe transportation 17.00 of laboratory animals around the world 17.00-17.30 Different caging systems for small laboratory rodents Grou ps Thursday 16 pril 2015 Friday 17 pril 2015 17.30-18.20 09.00-11.00 11.00-11.15 11.15-12.15 12.15-13.15 13.15-14.15 14.15-15.15 15.15-15.30 15.30-17.00 17.00-18.30 reeding Strategies for Maintaining Colonies of G Laboratory Mice Demonstration experimental techniques, collection of body fluids Mice nomenclature Lunch break Clinical / Physical Exam - Modified SHIRP + M.Raspa, M.Kamber, C. Johner,V.alafas,.Giannakop oulou Jorge Sztein J.Watson ehavioural Screen level (theory) + J.Watson ehavioural Screen level (practicals) J.Watson, M.Kamber, V.alafas,S.Mandillo,.Gianna kopoulou Preparation protocol, self-study T.Paparountas ehavioural Screen level J.Watson, M.Kamber, (practicals) V.alafas,S.Mandillo,.Gianna kopoulou Preparation protocol, self-study T.Paparountas 9.00-10.00 Genetic standardization C.Gross 10.00- nimal models of anxiety and fear C.Gross 10.45 10.45-11.00 11.00- Vasectomy (theory) +.Giannakopoulou 11.30 11.30-12.30 Vasectomy(practicals) Preparation protocol/self-study.giannakopoulou, M.Kamber V.Rossi, M.Riposati,M.Gaetani 12.30- Lunch break 13.30 13.30-14.30 Vasectomy (practicals).giannakopoulou, M.Kamber V.Rossi, M.Riposati,M.Gaetani Preparation protocol/self-study T.Paparountas 14.30-15.15 15.15-16.45 16.45-17.45 17.45-18.45 From the idea to the experiment Environmental influences on phenotypes guide for nimal facility Managers New Health surveillance techniques.papalois J.Watson S.ernardi J. Watson
WEEK 2 Time Lectures / practical / Groups Monday 20 pril 2015 Tuesday 21 pril 2015 09.00-11.00 Statistics + methodology I D.Lovell 11.00-11.15 11.15-12.45 Statistics + methodology II D.Lovell 12.45-13.45 Lunch break 13.45-15.15 Statistics + methodology III D.Lovell 15.15-17.15 Statistic IV D.Lovell 17.15-17.45 Zoonosis M. Kamber 17.45-18.30 High Resolution in vivo micro-imaging for small animals S.Lacerenza 09.00-10.30 Statistic Practical D.Lovell-T.Paparountas 10.30-10.45 10.45-12.15 Statistic Practical D.Lovell-T.Paparountas 12.15-13.15 Lunch break 13.15-14.45 Cost-enefit analysis G.Perretta 14.45-16.15 naesthesia Patricia Hedenqvist 16.15-16.30 16.30-17.30 nalgesia and peri-operative care Patricia Hedenqvist 22 pril 2015 17.30-18.30 lternatives to nimal procedures I.Dontas 09.00-10.00 Euthanasia Patricia Hedenqvist 10.00-11.30 Endotracheal intubation in practice part M.Kamber,V.alafas, Preparation protocol, Self study 11.30-12.00 12.00-13.30 Endotracheal intubation in practice part M.Kamber,V.alafas, Preparation protocol, Self study T.Paparountas 13.30-14.30 Lunch break 14.30-16.00 Endotracheal intubation in practice part M.Kamber,V.alafas, Preparation protocol, Self study T.Paparountas 16.00-16.30 16.30-18.00 Endotracheal intubation in practice part M.Kamber,V.alafas, Preparation protocol, Self study T.Paparountas
WEEK 2 Time Lectures / practical / Groups Thursday 23 pril 2015 9.00-10.00 Necropsy(theory) C.rayton 10.00-12.00 Necropsy, trimming: practical s C. rayton, M.Kamber, M.Gaettani, V.Castiglioni,S.Tatti,V.Ros si Preparation protocol/self-study T.Paparountas 12.00-13.00 Lunch break 13.00-15.00 Necropsy, trimming: practical s C. rayton, V.Castiglioni,M.Kamber, M.Gaettani,S.Tatti, V.Rossi Preparation protocol, Self study 15.00-16.00 asic rodent biology V.Castiglioni 16.00-16.15 16.15-17.15 Rodent pathology I:infectious diseases of mice and rats C.rayton Friday 24 pril 2015 17.15-18.15 Rodent pathology I:Non infectious diseases of mice and rats C.rayton 09.00-10.00 Gene editing in mice and rats Jean Cozzi 10.00-12.30 Examination + 12.30-13.30 Lunch break 13.30-16.30 Presentation protocol + C.rayton,T.Paparountas, C.Gross,G.Perretta,F.Scav izzi, 16.30-17.00 Vevo3100 demo Visualsonics in vivo Savino Lacerenza imaging applications for small animals 17.00-18.00 Refreshments and farewell ll