Compliance of manufacturers of AST materials and devices with EUCAST guidelines Data are based on questionnaires to manufacturers of materials and devices for antimicrobial susceptibility testing. The tables will be updated when manufacturers report changes (contact erika.matuschek@escmid.org). The accuracy of data in these tables is not verified by EUCAST and the inclusion of any materials or devices does not indicate endorsement by EUCAST. Last updated 10 March 2016
EUCAST disk diffusion method Manufacturer Disks available MH-F plates available Abtek BD Bioanalyse All except ceftobiprole 5 µg and ceftolozane-tazobactam 30-10 µg All except ceftobiprole 5 µg and ceftolozane-tazobactam 30-10 µg All except ceftaroline 5 µg, ceftobiprole 5 µg and ceftolozane-tazobactam 30-10 µg No Expected 2016 No Bio-Rad All except ceftobiprole 5 µg biomérieux No disks marketed I2a Liofilchem All except ceftaroline 5 µg and ceftobiprole 5 µg All except ceftolozane-tazobactam 30-10 µg (expected April 2016) No MAST Group All except ceftobiprole 5 µg No but supply β-nad supplement Thermo Fisher Scientific (Oxoid) All except ceftobiprole 5 µg, ceftolozane-tazobactam 30-10 µg and ticarcillin-clavulanic acid 75-10 µg Rosco All (Neo-Sensitabs) No
Automated systems for reading antimicrobial susceptibility testing plates Manufacturer System EUCAST breakpoints EUCAST Expert Rules EUCAST terminology S R> - Bio-Rad OSIRIS/ADAGIO Giles Scientific BIOMIC V3. There are additional rules but EUCAST rules have priority.. No exceptions I2a SIRSCAN: 2000 2000 automatic Micro. There are additional rules but none should be contrary to EUCAST rules. Synbiosis - Division of Synoptics ChromaZona. EUCAST rule number displayed. No No
Gradient tests: Etest (biomérieux) Manufacturer Agents in EUCAST tables but not available Agents validated for MH-F biomérieux Amoxicillin-clavulanic acid (fixed 2 mg/l clavulanic acid)*, ** Ampicillin-sulbactam (fixed 4 mg/l sulbactam)*, ** Cefadroxil Cefalexin Cefazolin Ceftibuten Ceftobiprole (available for resaerch use only) Ceftolozane-tazobactam (expected 2016) Oritavancin Roxithromycin Tedizolid Ticarcillin Ampicillin Amoxicillin Benzylpenicillin Cefaclor Cefepime Cefixime Cefotaxime Cefpodoxime Ceftriaxone Cefuroxime Doripenem Ertapenem Imipenem Meropenem Chloramphenicol Ciprofloxacin Levofloxacin Moxifloxacin Ofloxacin Azithromycin Clarithromycin Erythromycin Clindamycin Doxycycline Minocycline Tetracycline Tigecycline Teicoplanin Vancomycin Daptomycin Linezolid Nitofurantoin Rifampicin Trimethoprim Trimethoprim-sulfamethoxazole (not for H. influenzae and M. catarrhalis) *Gradient tests with the agent:inhibitor in a 2:1 ratio are not acceptable using EUCAST methods and breakpoints ** Expected during 2014
Gradient tests: MIC Test Strip (Liofilchem) Manufacturer Agents in EUCAST tables but not available Agents validated for MH-F Liofilchem Cefadroxil Cefalexin Roxithromycin Telithromycin Ticarcillin Amoxicillin Amoxicillin-clavulanic acid (fixed 2 mg/l clavulanic acid)* Ampicillin Ampicillin-sulbactam (fixed 4 mg/l sulbactam)* Azithromycin Benzylpenicillin Cefaclor Cefepime Cefixime Cefotaxime Cefpodoxime Ceftaroline Ceftriaxone Cefuroxime Cephalothin Chloramphenicol Ciprofloxacin Clarithromycin Clindamycin Doripenem Doxycycline Ertapenem Erythromycin Imipenem Levofloxacin Linezolid Meropenem Minocycline Moxifloxacin Ofloxacin Rifampicin Teicoplanin Tetracycline Trimethoprim-sulfamethoxazole Vancomycin *Gradient tests with the agent:inhibitor in a 2:1 ratio are not acceptable using EUCAST methods and breakpoints
Gradient tests: M.I.C.Evaluator (Thermo Fisher Scientific) Manufacturer Agents in EUCAST tables but not available Agents validated for MH-F Thermo Fisher Scientific (Oxoid) Amoxicillin-clavulanic acid (fixed 2 mg/l clavulanic acid)* Ampicillin-sulbactam (fixed 4 mg/l sulbactam)* Azithromycin Aztreonam Cefaclor Cefadroxil Cefalexin Cefazolin Cefepime Cefixime Cefoxitin Cefpodoxime Ceftibuten Ceftobiprole Ceftolozane-tazobactam Cefuroxime Chloramphenicol Clarithromycin Colistin Dalbavancin Doripenem Doxycycline Ertapenem Fosfomycin Fusidic acid Mecillinam Minocycline Moxifloxacin Mupirocin Nalidixic acid Netilmicin Nitrofurantoin Norfloxacin Ofloxacin Oitavancin Piperacillin Piperacillin-tazobactam Quinupristin-dalfopristin Rifampicin Roxithromycin Spectinomycin Streptomycin Tedizolid Telavancin Telithromycin Ticarcillin Ticarcillin-clavulanate Tobramycin Trimethoprim Trimethoprim-sulfamethoxazole Amoxicillin Ampicillin Benzylpenicillin Oxacillin Cefotaxime Ceftazidime Ceftaroline Levofloxacin Amikacin Teicoplanin Vancomycin Erythromycin Tetracycline Tigecycline Linezolid *Gradient tests with the agent:inhibitor in a 2:1 ratio are not acceptable using EUCAST methods and breakpoints
Phoenix/EpiCenter automated system (BD) EUCAST terminology In computer database S No (R ) - S EUCAST Expert Rules EUCAST organism groups with no test in the system Agents in EUCAST tables but not available in the system Agents available but EUCAST breakpoints not in the system In reports ( converted to ) - (MICs reported for agents with no EUCAST breakpoints) (MICs reported for agents with no EUCAST breakpoints) All rules incorporated. There are additional rules but EUCAST rules have priority. H. influenzae M. catarrhalis N. meningitidis N. gonorrhoeae Ampicillin-sulbactam (fixed 4 mg/l sulbactam)* Ceftobiprole Ceftolozane-tazobactam Gram-negative anaerobes Gram-positive anaerobes Rifampicin (Staphylococcus spp. cannot be reported susceptible) Trimethoprim (Enterococcus spp. cannot be reported susceptible) Trimethoprim-sulfamethoxazole (Enterococcus spp. cannot be reported susceptible) *Tests with the agent:inhibitor in a 2:1 ratio are not acceptable using EUCAST methods and breakpoints
MicroScan automated system (Beckman Coultier Diagnostics) S In computer database - No EUCAST terminology S No In reports - Most - do not have interpretations reported No EUCAST Expert Rules. There are other rules in the system but EUCAST rules take precedence for EUCAST panels. All rules can be customized by user. EUCAST organism groups with no test in the system M. catarrhalis Pasteurella N. meningitidis N. gonorrhoeae Gram-negative anaerobes Gram-positive anaerobes Agents in EUCAST tables but not available in the system Agents available but EUCAST breakpoints not in the system Doxycycline Roxithromycin Telithromycin Dalbavancin Oritavancin Tedizolid Chloramphenicol Fusidic acid Cefuroxime Ampicillin-sulbactam (fixed 4 mg/l sulbactam)* Amoxicillin-clavulanic acid (fixed 2 mg/l clavulanic acid)* Ceftobiprole Ceftolozane-tazobactam All agents for anaerobes Tigecycline (Gram-positive organisms) Rifampicin Trimethoprim *Tests with the agent:inhibitor in a 2:1 ratio are not acceptable using EUCAST methods and breakpoints
EUCAST terminology EUCAST Expert Rules EUCAST organism groups with no test in the system Agents in EUCAST tables but not available in the system Agents available but EUCAST breakpoints not in the system Compliance of manufacturers with EUCAST guidelines, 10 March 2016 VITEK 2 automated system (biomérieux) In computer database In reports S - S - H. influenzae Gram-negative anaerobes C. jejuni and coli M. catarrhalis Gram-positive anaerobes Corynebacterium spp. N. meningitidis H. pylori M. tuberculosis N. gonorrhoeae L. monocytogenes Azithromycin Roxithromycin Cefuroxime (S. pneumoniae ) Dalbavancin Ampicillin-sulbactam (fixed 4 mg/l sulbactam) * (Staphylococcus spp. and Enterococcus spp.) Phenoxymethylpenicillin Cefadroxil Ceftobiprole Ceftibuten Ceftolozane-tazobactam (expected during 2016) Daptomycin Doxycycline (S. pneumoniae ) ICR (S. pneumoniae, Viridans group, Strep. Gr. C & G) Minocycline (S. pneumoniae ) Oritavancin Tedizolid Telavancin Cefepime (S. pneumoniae) Cefpodoxime (S. pneumoniae) Netilmicin (Staphylococcus spp.) Trimethoprim (Enterococcus spp. and group B streptococci) Amoxicillin-clavulanic acid (fixed 2 mg/l clavulanic acid)* uncomplicated UTI breakpoints (Enterobacteriaceae) Doripenem (Enterobacteriaceae, Pseudomonas spp., Acinetobacter spp.) Ciprofloxacin (Salmonella spp. and Enterococcus spp.) Levofloxacin (Enterococcus spp.) Trim-sulfa (Enterococcus spp.) Ofloxacin (S. pneumoniae ) Streptomycin (Enterococcus spp.) Antifungals All agents with P. multocida Benzylpenicillin (CoNS) Cefoxitin screen (S. saprophyticus and S. pseudintermedius) Norfloxacin (Enterococcus spp.) *Tests with the agent:inhibitor in a 2:1 ratio are not acceptable using EUCAST methods and breakpoints All applicable rules dealing with intrinsic resistance, therapeutic interpretation and deduction are beginning with software version V2S 5.03 (2011). There are additional rules but none should be contrary to EUCAST rules in this software version.
Sensititre (Thermo Fisher Scientific) EUCAST terminology In computer database S - S EUCAST Expert Rules EUCAST organism groups with no test in the system Agents in EUCAST tables but not available in the system Agents available but EUCAST breakpoints not in the system In reports - All EUCAST rules are in software version 3.3 (2014). There are additional rules, but these rules are inactivated in the EUCAST setting. N. gonorrhoeae All agents available All agents for yeasts and molds
MICRONAUT (MERLIN Diagnostika GmbH) EUCAST terminology In computer database S - No S EUCAST Expert Rules EUCAST organism groups with no test in the system Agents in EUCAST tables but not available in the system Agents available but EUCAST breakpoints not in the system In reports - (MICs reported for agents with no EUCAST breakpoints) No (MICs reported for agents with no EUCAST breakpoints) All EUCAST rules are. There are additional rules in the software. Our rules can be customized by the user. N. gonorrhoeae Ceftolozane-tazobactam (expected during 2016) All agents for molds.
MIKROLATEST (Erba Mannheim) EUCAST terminology In computer database S - (I) S EUCAST Expert Rules EUCAST organism groups with no test in the system Agents in EUCAST tables but not available in the system Agents available but EUCAST breakpoints not in the system In reports - (I) EUCAST rules are in software version 20151105 (2015). There are additional rules in the software. The rules can be customized by the user. Gram-positive anaerobes N. gonorrhoeae Gram-negative anaerobes H. influenzae All agents for anaerobes, Corynebacterium spp., N. gonorrhoeae, H. influenzae None Corynebacterium spp.