Recent Topics of Brucellosis Koichi IMAOKA BrucellosisBrucella spp. 1999 4 1 2008 12 31 13 4 9 2007 6 1 Brucella, B. abortus, B. suis, B. canis 19 1887 Bruce Micrococcus Brucella B. biovar... B. B. suisb. abortus 0162-8640 1-23 -1 Department of Veterinary Science, National Institute of Infectious Diseases (1-23-1 Toyama, Shinjuku-ku, Tokyo)
B. canis B. pinnipedialisb. ceti B. ovis B. neotomaeb. microti 1 3 B. B. abortus B. suis smooth-typelps o-side chain B. canis rough-type LPS o-side chain 1 3 40 1 2 5 1, 3, 4 B. B. abortus 1-6, 9 B. 1-3 B. suis 1, 3 2 4B. rangiferi 5 B. canis B. ovis B. neotomae B. pinnipedialis B. ceti B. microti 1 1 3 40 1 2 1
B. 500 473 464 457 437 431 410 403 388 326 241 234 225 202 185 160 95 93 91 87 86 82 81 78 65 48 47 45 40 37 32 125, 97, 20, 62 290 1 B. abortusb. B. suisoie OIE 1 1, 2, 5 B. abortus 17 90 B. 16 50 1, 2, 5 7 8 2 50 1, 4, 6 B. 2 4 8 9 1 2 B.ovis OIEMultiannual Animal Diseases Status 5
1940 B. abortus 1960 1970 B. suis 100 B. B. canis 8 B. canis 10 12 1 B. abortus 1916 1953 1947 1972 4596 B. B. suis 1940 7 B. canis 1971 13, 14 2 5B. canis 14, 15 2 1999.3.31 1999.3.31 1933 B. abortus 16 1962 1933 1962 17 51 3 13 35 6 B. abortus 18 19 B. 20, 21 20 21, 22 B. canis 1 23 1970 B. canis 3.9 401,017 30.4723 1971 70 70 8.8 2 5 2 51114 1683 139263 1537 1859 2
2.0693,440 14 B. canis 31999.4.1 2008.12.31 13 12 2005 4 B. abortuss-lps 24, 25 B. 2 26, 27 B. aboruts 2 16 17 18 23 20 20 19 18 21 22 PCR PCR PCR 1933 1933 1962 1976 1977 1980 1981 1993 1995 1998 3 13 35 32 51 34 17 6 41 38 1 7 64 60 abortus suis abortus abortus canis abortus abortus 1999.3.31 2002.1 2005.6 2005.12 2006.2 2006.6 2006.7 2006.9 2006.10 2007.4 2008.6 2008.7 2008.8 2008.8 abortus abortus PCR canis canis canis abortus 1999.4.1 2008.12.31 B.abortusB.canisB.abortus40B.canis160 24
B. B. aboruts 9 B. canis B. canis 5 24, 25 2008 8 2 B. canis B. canis 28 1B. 2005 6 26 30 38 40 16S rrna BA : 160 BC : 320 428 57 62 64 66 40 613 A 625 625 729 84 DOXY GM 2B. canis 2008 8 28 1 70 89 818 2 40 819 825 B. canis PCRB. canis 1 1280 2 320 DOXY SM DOXY RFP 37 B. canis 14 5 B. canis DOXY RFP 10 100 1 4, 6 B. canis 25 6 29 11, 30
1 2 3 4 5 B. canis 1 2 3 90 21 24, 71.1 38 25 37 0 4 8 4 5 2 3 3 5 6 4 4 3 5 11 9 24 18 6 3 4 10 90 180 6 40 160 PCR Moraxella phenylpyruvica 1 1, 3, 7 1 SAT 7, 31 smooth-lps B. abortus rough- LPS B. canis B. canis 7 3 4 Yersinia enterocolitica O9
100 Ochrobactrum intermedium MAT MAT SAT 15 SAT 2 1 3, 7, 8 21 2 Trypticase Soy AgarTS Agar 5 Sheep Blood AgarSBASerum Dextrose Agar5 vv equinebovine serum1 wv dextrosesmoothtype roughtype 3 1.5 2mm 0.4 x 0.8um 1, 7 3 PCR 1, 2, 32, 33 B. abortus BCSP31 PCR 32 16S ribosomal RNA IS711 BCSP31 2 OMP2OMP31 PCR 4 7, 33 1, 34 36 1986 WHO DOXY RFP 34 1, 35 RFP DOXY 2 WHO 1986 1 100mg 242 8 ST WHO 1986 8mgkg 40mgkg 5 26 2 5mgkg710 3 1g11421 4 15mgkg600900mg42 8mgkg40mgkg 2 6 15mgkg600900mg 45 15mgkg600900mg42 30mgkg 5mgkg 13534 123 45
DOXY SM GM SM DOXY GM GMSM RFP 36 2 3 DOXY GM RFP 1 1 B. canis B. canis 37 1 Brucellosis in humans and animals. WHOCDSEPR 2006.7.http:www.who.intcsrresourcespublications deliberatewho_cds_epr 2006_7enWHO, 2006. 2 Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2008.http:www.oie.intengnormesmmanual A_summry.htmOIE, 2008. 3 Brucellosis.http:emergency.cdc.govagentbrucellosis CDC, 2008. 4 Pappas G., Papadimetriou P., Akritidis N., et al.: The new global map of human brucellosis. Lancet Infect Dis, : 91-99, 2006. 5 Multiannual animal disease status, Bovine brucellosis. Caprine and ovine brucellosisexcluding B. ovis. http:www.oie.inths2report.asp?lang=enoie, 2004. 6 Memish Z.A., H.Balkhy H.H.: Brucellosis and international travel. J Travel Med, : 49-55, 2004. 7.. in,, 95-108, 2008 8 Greene, C.E. and Carmichael, L.E. Canine brucellosis. pp369-381. in : Greene CE.ed, Infectious diseases of the dog and cat, 3rd ed. Elsevier, Inc., Canada, 2006. 9. in :,,, 8: 228-229, 2007. http:idsc.nih.go.jpiasr28330fr3301.html 10Munford R.S., Weaaver R.E., Patton C., et al.: Human disease caused by Brucella canis. A clinical and epidemiologic study of two cases. JAMA, : 1267-1269, 1975. 11Polt S.S., Dismukes W.E., Flint A., et al.: Human brucellosis caused by Brucella canis. Clinical features and immune response. Ann Intern Med, : 717-719, 1982 12Lucero N.E., Jacob N.O., Ayala S.M., et al.: Unusual clinical presentation of brucellosis caused by Brucella canis. J Med Microbiol, : 505-508, 2005. 13,,.., :175-192, 1974. 14.., : 97-101, 1994. 15Kimura, M., Imaoka, K., Suzuki, M., et al.: Evaluation of a microplate agglutination testmatfor serological diagnosis of canine brucellosis. J. Vet. Med. Sci, : 707-709, 2008. 16.., : 23-24, 1933. 17...., : 201-204, 1962. 18,,. Brucella abortus 4 1., : 1067-1070, 1997. 19Takahashi, H., Tanaka, S., Yoshida K., et al.: An unusual case of brucellosis in Japan : Difficulties in the differential diagnosis from pulmonary tuberculosis. Internal Med., : 310-314, 1996. 20,,. Brucella biotype 3., : 336, 1982. 21,,. 2., : 953-956. 1999.
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