Med J Chin PL, Vol., No., pril, 8 89 [ ]() PC g/ml PC6 h 6(IL-6) (TNF- ) Toll(TLR) 88(MyD88) p-p65( 6h) () (g/ml ) ( mol/l ) ( g/ml mol/l ) IL-6 TNF-6 h (P<.5) 6h PCTLR/MyD88/NF- B 6hPC IL-6 TNF- TLR MyD88 p-p65 (P<.5) PC [] PCToll [ ] R6 R76.6 [ ] [ ] 577-7(8)-89-5 [DOI].855/j.issn.577-7.8.. Protective role and mechanism of dexmedetomidine on the -stimulated PC cells ZHOU Jun-jie, XIO Wei, HE Xuan, PENG Na, TONG Hua-sheng *, SU Lei Department of Intensive Care Unit, Heyuan People's Hospital ffiliated to Jinan University, Heyuan, Guangdong 57, China Department of Intensive Care Unit, Guangzhou General Hospital of Guangzhou Military rea Command, Guangzhou 5, China * Corresponding author, E-mail: fimmuths@6.com This work was supported by the Doctoral Start-up Projects of Natural Science Foundation of Guangdong Province (688) [bstract] Objective To explore the effect of dexmedetomidine on the lipopolysaccharide () stimulated PC cells and its potential mechanism. Methods PC cells were treated by with a concentration of g/ml. The cell viability, the concentrations of interleukin-6 (IL-6) and tumor necrosis factor (TNF- ) in the cell culture supernatant were measured after -, 6-, or -h treatment. The expressions of toll-like receptor (TLR), myeloid differentiation factor 88 (MyD88) and phosphorylated p65 (p-p65) were measured. In the second part, PC cells were cultured under four different treatments, that is, normal culture media in first group, g/ml in second group, mol/l dexmedetomidine in third group, g/ml and mol/l dexmedetomidine in fourth group. The indexes mentioned above were measured 6 hours after and treatments. Results The cell viability was decreased after treatment, and the concentrations of IL-6 and TNF- were increased significantly. Compared with control group, the concentrations in -, 6-, -h groups showed statistically significant differences (P<.5), especially after 6 hours. The TLR/MyD88/NF- B pathway was activated after stimuli and reached the peak value. Compared with treatment group, PC cell apoptosis rate, the concentrations of IL-6 and TNF- and the expressions of TLR, MyD88 and p-p65 were decreased. The differences between group and group was statistically significant (P<.5). Conclusion Dexmedetomidine has a protective effect on stimulated PC cells via the inhibition of inflammatory response. [Key words] lipopolysaccharide; dexmedetomidine; PC cells; Toll-like receptor [ ] (688) [ ] MODS [ ] 57 () 5 () [ ] E-mail fimmuths@6.com
9 8 [] [] [] [] (lipopolysaccharide ) [5] Tol l (Tolllike receptors TLR) TLR (pathogen-associated molecular patterns PMPs) TLR88 (myeloid differentiation factor 88 MyD88) NF- B [6] (dexmedetomidine ) [7] ICU [8-9] [] PC. MTT Hoechst MPDMEM GibcoTNF- IL-6 ELIS ebiosiencetlr MyD88 Cell Signaling Technology.PC 7 5% CO % %- DMEM h 6 h [] g/ml 6 h g/ml [] mol/l g/ml mol/l.96 MTT 96.5mg/ml MTT 5 l 7 h MTT DMSO 5 l min 9nm(OD) = OD /OD %. Hoechst % 5min PBS g/ml Hoechst 7min 6nm nm.5 TNF- IL-6 5nm OD TNF- IL-6.6TLR MyD88 p-65 p-p65 Western blotting BCSDS-PGE TLR(:) MyD88(:) p-65(:) p-p65(:) (:5) Image J.7SPSS. x±s LSD-tP<.5. PCIL-6 TNF- g/ml PC 6h h 6h (P<.5) IL-6 TNF- 6 h (P<.5) 6h ( ). PC TLR MyD88 p-p65 Western blotting PC TLR MyD88 p-p65 ( ) 6h IL-6 TNF- 6h
Med J Chin PL, Vol., No., pril, 8 9 5 6 8 6 5 B C PC () IL-6(B) TNF- (C) Fig. Effects of on PC cell viability (), concentrations of IL-6 (B) and TNF- (C) at different time points Cell viability (%) TLR GPDH IL-6 (pg/ml) P<.5 compared with control group MyD88 GPDH B p65 C PC TLR() MyD88(B) p-p65(c) Fig. Effects of on the expressions of TLR (), MyD88 (B) and p-p65 (C) in PC cells at different time points TNF- (pg/ml) p-p65. PC g/ml mol/l PC (P<.5) Hoechst ( B) PC Cell viability (%) 5 5 B PC 6h () (B, Hoechst staining, ) Fig. Effects of dexmedetomidine on the cell viability () and apoptosis rate (B, Hoechst staining, ) of -treated PC cells P<.5 compared with control group; P<.5 compared with group. PC IL-6 TNF- IL-6 7.5 5.pg/ml 58.7.pg/ml (P>.5)6h 65.6 59.7pg/ml (P<.5) 6h IL-68.5.5pg/ml ( P <.5) TNF- IL-6 (pg/ml) 8 6 6.9.89pg/ml 55.87 7.9pg/ml ( P >.5)6h 76.7 7.9pg/ml(P<.5) 6h TNF- 5. 6.99pg/ml (P<.5 ) PC.5 PC TLR MyD88 p-p65 Western blotting PC 6h IL-6() TNF- (B) Fig. Effects of dexmedetomidine on the concentrations of IL-6 () and TNF- (B) in the supernatants of -treated PC cells P<.5 compared with control group; P<.5 compared with group TNF- (pg/ml) 6 B
9 8 PCTLR MyD88 p-p65 Image J TLR/GPDH TLR GPDH 5 MyD88/GPDH MyD88 GPDH (P<.5 5) B C 5 PC 6hTLR() MyD88(B) p-p65(c) Fig.5 Effects of dexmedetomidine on the expressions of TLR (), MyD88 (B) and p-p65 (C) in -treated PC cells p-p65/p65 p-p65 p65 P<.5 compared with control group; P<.5 compared with group 5 [] ICU - [-7] 8d [8] [9] TNF- IL-6 IL-8 [-] Ning [] TNF- IL-6 PC TLR TLR/MyD88/NF- B TNF- IL-6 Toll TLR Toll [-] TLR (PMPs) TLR MyD88 IL-(interleukin- receptor associated kinase IRK) 6(TNF receptor associated factor 6 TRF6) NF- B TLR/MyD88/NF- B TNF- IL-6 Liu [] TLR/MyD88/NF- B [5-6] TNF- IL- [7] TLR/MyD88/NF- B TNF- IL-6 c-jun TNF- IL-6 [8] PC TNF- IL-6
Med J Chin PL, Vol., No., pril, 8 9 [] Singer M, Deutschman CS, Seymour CW, et al. The third international consensus definitions for sepsis and septic shock (sepsis-)[ J]. JM, 6, 5(8): 8-8. [] Kuperberg SJ, Wadgaonkar R. Sepsis-associated encephalopathy: the blood-brain barrier and the sphingolipid rheostat[ J]. Front Immunol, 7, 8: 597. [] Feng Q, i YH, Gong H, et al. Characterization of sepsis and sepsis-associated encephalopathy[ J]. J Intensive Care Med, 7, doi:.77/88566677975. [] Pandharipande PP, Girard TD, Ely EW. Long-term cognitive impairment after critical illness[ J]. N Engl J Med,, 7: 85-86. [5] Faraj T, McLaughlin CL, Erridge C. Host defenses against metabolic endotoxaemia and their impact on lipopolysaccharide detection[ J]. Int Rev Immunol, 7, 6(): 5-. [6] P óciennikowska, Hromada-Judycka, Borz cka K. et al. Co-operation of TLR and raft proteins in -induced proinflammatory signaling[ J]. Cell Mol Life Sci, 5, 7(): 557-58. [7] Tang C, Xia Z. Dexmedetomidine in perioperative acute pain management: a non-opioid adjuvant analgesic[ J]. J Pain Res, 7, (): 899-9. [8] Tran, Blinder H, Hutton B. et al. systematic review of alpha- agonists for sedation in mechanically ventilated neurocritical care patients[ J]. Neurocrit Care, 8, 8: -5. [9] Wang JX, Shi SZ, L i JH, et al. pplication effects of dexmedetomidine combined with sevoflurane for anesthesia in short operations in children with burn injury[ J]. Med J Chin PL, 6, (): 5-57. [,,,. [ J]., 6, (): 5-57.] [] Ning Q, Liu Z, Wang X, et al. Neurodegenerative changes and neuroapoptosis induced by systemic lipopolysaccharide administration are reversed by dexmedetomidine treatment in mice[ J]. Neurol Res, 7, 9(): 57-66. [] Wang H, Xu YS, Wang ML, et al. Protective effect of naringin against the -induced apoptosis of PC cells: Implications for the treatment of neurodegenerative disorders[ J]. Int J Mol Med, 7, 9(): 89-8. [] Wang Q, Tan Y, Zhang N. et al. Dexmedetomidine inhibits activation of the MPK pathway and protects PC and NG8-5 cells from lidocaine-induced cytotoxicity at its maximum safe dose[ J]. Biomed Pharmacother, 7, 9: 6-66. [] Schmutzhard E, Pfausler B. Neurologic complications of sepsis [ J]. Handb Clin Neurol, 7, : 675-68. [] Shen M, Wang S, Wen X, et al. Dexmedetomidine exerts neuroprotective effect via the activation of the PIK/kt/mTOR signaling pathway in rats with traumatic brain injury[ J]. Biomed Pharmacother, 7, 95: 885-89. [5] Jiang L, Hu M, Lu Y, et al. The protective effects of dexmedetomidine on ischemic brain injury: a meta-analysis[ J]. J Clin nesth, 7, : 5-. [6] Luo C, Ouyang MW, Fang YY, et al. Dexmedetomidine protects mouse brain from ischemia-reperfusion injury via inhibiting neuronal autophagy through up-regulating HIF- [ J]. Front Cell Neurosci, 7, : 97. [7] Li Y, Liu S. The effect of Dexmedetomidine on oxidative stress response following cerebral ischemia-reperfusion in rats and the expression of intracellular adhesion molecule- (ICM-) and SB[ J]. Med Sci Monit Int, 7, : 867-87. [8] Zamani MM, Keshavarz-Fathi M, Fakhri-Bafghi MS, et al. Survival benefits of dexmedetomidine used for sedating septic patients in intensive care setting: a systematic review[ J]. J Crit Care, 6, : 9-. [9] Yeh YC, Wu CY, Cheng YJ, et al. Effects of Dexmedetomidine on intestinal microcirculation and intestinal epithelial barrier in endotoxemic rats[ J]. nesthesiology, 6, 5: 55-67. [] Liu Z, Wang Y, Wang Y, et al. Dexmedetomidine attenuates inflammatory reaction in the lung tissues of septic mice by activating cholinergic anti-inflammatory pathway[ J]. Int Immunopharmacol, 6, 5: -6. [] Tan F, Chen Y, Yuan D, et al. Dexmedetomidine protects against acute kidney injury through downregulating inflammatory reactions in endotoxemia rats[ J]. Biomed Rep, 5, (): 65-7. [] Wu BW, Zhu J, Shi HM, et al. ssociation between Toll-like receptor sp99gly polymorphism and coronary heart disease susceptibility[ J]. Braz J Med Biol Res, 7, 5(9): e66. [] Bachar O, dner M, Uddman R, et al. Toll-like receptor stimulation induces airway hyper-responsiveness to bradykinin, an effect mediated by JNK and NF-kappa B signaling pathways[ J]. Eur J Immunol,, (): 96-7. [] Liu X, Xiao Q, Zhao K, et al. Ghrelin inhibits high glucoseinduced PC cell apoptosis by regulating TLR/NF- B pathway[ J]. Inflammation,, 6(6): 86-9. [5] Corrigan F, rulsamy, Collins-Praino LE, et al. Toll like receptor activation can be either detrimental or beneficial following mild repetitive traumatic brain injury depending on timing of activation[ J]. Brain Behav Immun, 7, 6: -9. [6] Wu D, Zhang X, Zhao M, et al. The role of the TLR/NF- B signaling pathway in accumulation in primary hippocampal neurons[ J]. cta Physiologica Sinica, 5, 67(): 9-8. [,,,. TLR/NF- B [ J]., 5, 67(): 9-8.] [7] Basu S, garwal P, nupurba S, et al. Elevated plasma and cerebrospinal fluid interleukin- beta and tumor necrosis factor-alpha concentration and combined outcome of death or abnormal neuroimaging in preterm neonates with early-onset clinical sepsis[ J]. J Perinatol Off J Calif Perinat ssoc, 5, 5(): 855-86. [8] Zhang X, Wang J, Qian W, et al. Dexmedetomidine inhibits tumor necrosis factor-alpha and interleukin 6 in lipopolysaccharidestimulated astrocytes by suppression of c-jun N-terminal kinases[ J]. Inflammation,, 7(): 9-99. ( 7--68--) ( )