Anaesthesia of farmed fish

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Bergen 6th of March 2018 Anaesthesia of farmed fish implications for welfare Solveig M R Nygaard Veterinarian FoMAS- Fiskehelse og Miljø as

Our job: visiting farms, inspecting fish, sampling, consultancy due to treatments, training courses etc

FoMAS Fiskehelse og Miljø as - Fish health service and environmental tests 5 offices 13 employees, 10 veterinarians and fish health biologists

FoMAS Fiskehelse og Miljø AS Solveig M R Nygaard Daglig leder Veterinær spesialist fisk Haugesund Tlf: 90 19 76 16 solveig@fom as.no Endre Karlsen Fiskehelsebiolog Os fra 1. april Tlf: 95784801 endre@fom as.no Bjarte Langhelle Fiskehelsebiolog Os Tlf: 48954006 bjarte@fom as.no Stine Kolstø Fiskehelsebiolog Haugesund Tlf: 97 60 05 71 stine@fom as.no Siri Giskegjerde Veterinær Forsand Tlf: 90 03 28 53 siri@fom as.no Marianne Rauboti Viken veterinær Os O Begynner 22. mai 2017 marianne@fom as.no Hanna Sæteraas Bjerke Veterinær Haugesund Tlf: 48 02 54 98 hanna@fom as.no Herman Høgenes Kvinnsland Fiskehelsebiolog Haugesund herman@fom as.no Sverri Strøm Veterinær Haugesund Tlf: sverri@fom as.no Britt Kari Legård Veterinær Odda Tlf: 97521071 Britt.kari@fom as.no Stig Johar Øverland Adm. koordinator MSc bioteknologi Haugesund Tlf: 95935502 stig@fom as.no Ragnhild Haaland Malkenes Sekretær lusenettverk Høyskolekandidat Tysnes Tlf: 48253487 ragnhild@fom as.no Vibeke Sælen Helgesen Bioingeniør Tysnes Tlf: 91 17 25 43 vibeke@fom as.no

All staff on the fish farms has to take training courses in fish welfare every 5. year,- includes pain, how to use pharmaceuticals, killing etc

Consciousness- how to evaluate? Mammals Fish Sedation/use Reduced reaction to stimuli Reduced reaction to stimuli of tranquilizer Exitation Restless, spasms, Not usual in fish, may be increased activity of operculae Anaestetized Relaxed muscles, loss of reactions Relaxed muscles, loss of reactions Collapse, death Stopped breathing and heart beating Stopped movement of operculae, stopped heart beating

Still conscious, Not acceptable anaestetized Eye rolling Flat eye The eye is rolling

Tranquilizers few possibilities: Research stations: Aquacalm Metomidate hydrochloride (not for food production) NB: CO2 virker også sederende

Anaesthetics - Finquel (Tricainmesilat) withdrawal: 25 ddegrees Tricaine Pharmaq (Tricainmesilat) withdrawal: 70 ddegrees Nytox vet (Tricainmesilat) withdrawal: 70 ddegrees Benzoak (benzocaine 200 mg/ml) withdrawal: 70 ddegrees

Stunning, - good anaesthetics linked to killing

Electricity Acceptable anaesthetics when killing (Kjølaas / Seaside )

When do we need anaesthetics in the production in salmon hatcheries? h Vaccination Euthanasia

Why anaesthetize before vaccination? - Injection may be painful - The fish has to be anaestizide to achieve correct injection point Injection too high and not in midline Result: vaccine too close to liver

Anaesthetics before injection vaccination Size salmon and rainbowtrout: 30-150 gram

Our practice based on experiences: - best to achieve effect after 40-50 seconds, max 60 seconds - the dosage has to be adapted to temperature,- 3 x higher at low temp - rapid transfer from anaesthetic bath to water immersion - control oxygen level in anaestetic bath - supervision of wake up we prefer long pipes and that the fish is awake coming into the tank

Challenges: - Anaesthesize batches of fish and get them out of the bath at the right point - Pharmaceutical SPC does not support our practice in details -only 1 of 4 SPC `s advise to adapt dosage to temperature -metacaine: safety not documenteddtemp < 7 C and > 17 C -Anaesthetic bath turns slimy and dirty:- trend: rapid changes of anaesthetics

Buffer + metacaine - used in fresh water to avoid ph drop Experience from resirculation farms: have eliminated buffer after checking ph : drop not registered

Vaccination lumpsucker: neither sedation or Anaesthetics used (we have tried )

Cultivation pit tag

Research stations: - Bottleneck when small groups are transferred to small tanks - Ulcer problems, mortality - Prevent by using sedation, focuse on water temperatures, equipment etc

When do we need anaesthetics i salmon sea production? Lice counting

Sedation in sea water production linked to fresh water treatments / delousing - Useful at high temperatures, reduces stress and oxygen consumption -Always used in freshwater treatments at temperatures > 5-6 C Risks: too lazy fish at low temperatures, clog together toget e

Salmon broodfish

Grading broodfish 8-12 kg - Anaesthetics necessary to read pit tags, find weight, length, female/male l Brønnbåt i hardt vær. Foto: Leif Torkels

-Grading for maturation in the tanks -Sedation with AquiS useful Brønnbåt i hardt vær. Foto: Leif Torkels

Adding AquiS 15-20 minutes before grading

Anaesthetics in male broodfish before stripping necessary every male used 5 10 times

Euthanasia female brood fish before bleeding and stripping - Need really high consentrations at low temperatures Brønnbåt i hardt vær. Foto: Leif Torkels

Summary: - Anaesthetics in fish farming important to chieve good fish welfare - Tranquilizer useful in some operations - Important with quick effect of anaesthetics and following immersion or bath to clean the gills from anaesthetics - Avoid too long time in anaesthetic bath