TEST REPORT. Client: M/s Ion Silver AB. Loddekopinge. Sverige / SWEDEN. Chandran. min and 30 min. 2. E. coli. 1. S. aureus

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1 TEST REPORT TEST TYPE: Liquid Suspension Time Kill Study -Quantitative Test Based On ASTM 2315 TEST METHOD of Colloidal Silver Product at Contact time points: 30 sec, 1 min, 2 min, 5 min, 10 min, 15 min and 30 min. against following microorganisms: Bacterial cultures: 1. S. aureus 2. E. coli 3. Methicillin Resistant S. aureus 4. P. aeruginosa 5. K. pneumoniae 6. Enterococcus faecalis Fungal cultures: 1. Candida albicans 2. Aspergillus niger SAMPLE DETAILS No. of samples receipt date B. 2 x500 ml No: CAS -10PPM One SAMPLE DESCRIPTION: Two amber colored bottles.

2 TEST INFORMATION: Analysis Started on Analysis Completed on B. No: CAS Contact time 30 sec, 1 min, 2 min, 5 min, 10 min, 15 min and 30 min. Vol. of used Diluent Used 9.9 ml PBS Vol. of Inoculum used Neutralizer used 0.1 ml Dey Engley Neutralizing Broth METHODS USED: METHOD STANDARD: Time Kill Study -Quantitative Test Based On ASTM 2315 Test Method Based on ASTM MEDIA USED: Soyabean Casein Digest Broth Yeast Malt Broth DE Broth Soyabean Casein Digest Agar Potato Dextrose Agar Phosphate Buffered Saline (PBS) Phosphate Saline 0.85% with 0.2 % Tween 80 CULTURE USED: 1. Staphylococcus aureus ATCCC Klebsiella pneumoniae ATCC Escherichia coli ATCC Methicillin Resistant S. aureus ATCC P. aeruginosa ATCC Enterococcus faecalis ATCC Fungal cultures: 1. Candida albicans ATCC Aspergillus niger ATCC INCUBATION CONDITIONS C, Aerobic for hrs for Bacteria C Aerobic for hrs for Fungi.

3 I INITIAL INNOCULUM COUNT Staphylococcus aureus ATCC 6538 Klebsiella pneumoniae ATCC Escherichia coli ATCC 8739 Methicillin Resistant S. aureus ATCC P. aeruginosa ATCC 9027 Enterococcus faecalis ATCCC Candida albicans ATCC Aspergillus niger ATCC16404 CFU/ml 1.5 x x x x x x x x 10 8

4 II Test Microorganism Concentration Contact Time CFU / ml Log reduction vs. at Percent Reduction vs. at 4.1 E E E S. aureus ATCC 6538 B. No: CAS E E % 6.0E % Klebsiella pneumoniae ATCC B. No: CAS 5.0E E % > % 4.0E > % > % 6.95 > %

5 Test Microorganism Concentration Contact Time CFU / ml Log reduction vs. at Percent Reduction vs. at 2.0 E E % 2.0E % Escherichia coli ATCC 8739 B. No: CAS E E % 7.0E % Methicillin Resistant S. aureus ATCC B. No: CAS 1.8E E % %

6 Test Microorganism Concentration Contact Time CFU / ml Log reduction vs. at Percent Reduction vs. at 4.0 E E % 2.0E % P. aeruginosa ATCC 9027 B. No: CAS E E % Enterococcus faecalis ATCC B. No: CAS 1.1E E E % % % 4.0E % 1 4.0E % 3.0E %

7 Test Microorganism Concentration Contact Time CFU / ml Log reduction vs. at Percent Reduction vs. at 3.1 E E % % Candida albicans ATCC B. No: CAS 1.0E E % % 2.0E % 1 1.3E % 8.0E % 4.0 E+05 Aspergillus niger ATCC B. No: CAS 2.0E E E % 1 2.0E+05

8 RESULTS: Under the given test conditions: 1. The Test - B. No: CAS exhibits more than count of Staphylococcus aureus ATCC 6538 at of contact time. 2. The Test - B. No: CAS exhibits more than count of Klebsiella pneumoniae ATCC at. of contact time. 3. The Test - B. No: CAS exhibits more than count of Escherichia coli ATCC 8739 at of contact time. 4. The Test - B. No: CAS exhibits more than count of Methicillin Resistant S. aureus ATCC at of contact time. 5. The Test - B. No: CAS exhibits more than count of P. aeruginosa ATCC 9027 at of contact time. 6. The Test - B. No: CAS exhibits 98.50% reduction in the count of Enterococcus faecalis ATCC after of contact time. 7. The Test - B. No: CAS exhibits % reduction in the count of Candida albicans ATCC after of contact time. 8. The Test - B. No: CAS exhibits 75.00% reduction in the count of Aspergillus niger ATCC16404 after of contact time. Report prepared by: MAA Checked by: AGA Disclaimer Shubhda Research Institute does not assume any responsibility as regards the origin of the sample, resultss obtained or further actions taken by you based on our results. EOD