PESTE DES PETITS RUMINANTS (PPR) IN SAIGA ANTELOPE IN MONGOLIA

Transcription

1 PESTE DES PETITS RUMINANTS (PPR) IN SAIGA ANTELOPE IN MONGOLIA BODISAIKHAN.Kh State Central Veterinary Laboratory, Mongolia Bali, Indonesia

2 CONTENT About Saiga antelope of Mongolia Mortality of saiga antelope Laboratory diagnosis Comments

3 ABOUT SAIGA ANTELOPE OF MONGOLIA Central Asian antelopes 400,000 2,700,000 40, ,000 67,000-72,000 Richard Kock

4 ABOUT SAIGA ANTELOPE OF MONGOLIA Richard Kock & SCVL & WCS

5 ABOUT SAIGA ANTELOPE OF MONGOLIA

6 ABOUT SAIGA ANTELOPE OF MONGOLIA Mongolian Saiga is distributed in western part of Mongolia. Mongolian saiga has about 14,000 in 2013.

7 ABOUT SAIGA ANTELOPE OF MONGOLIA Populaton of saiga in Mongolia In recent years, Mongolian saiga population has increased steadily.

8 MORTALITY OF SAIGA ANTELOPE Mortality of Saga antelope There are too many saiga died in western part of Mongolia. We destroyed about 4000 saiga carcasses by official information. Unofficial information, about from 5 to 6 thousand saiga were died.

9 MORTALITY OF SAIGA ANTELOPE

10 MORTALITY OF SAIGA ANTELOPE We finded many many saiga carcasses on the frozen desert range Richard Kock & SCVL & WCS

11 MORTALITY OF SAIGA ANTELOPE Our country morbidity livestock by PPR at This disease covered western provinces of Mongolia. I b e x

12 MORTALITY OF SAIGA ANTELOPE Red cycle is Khovd province area. Blue cycle is Gobi-Altai province area.

13 MORTALITY OF SAIGA ANTELOPE PPR disease detected in Mongolia from wild animals. Saiga Black-tailed gazelles By June 2017 reported the death of saiga has been stopped, but the ibex died in mountains area (in Khovd province). Wild sheep Ibex

14 LABORATORY DIAGNOSIS BSL III Laboratory of TAD division Our organization have BSL-III laboratory. We used this laboratory to diagnose the PPR and TAD diseases. We used 3 kind of diagnostic kit and to give a result in 3-6 hours for PPR.

15 LABORATORY DIAGNOSIS The method of PPR diagnosis Prepare samples: After take a samples from field, to extracted gr sample Evolution, Bertin, France). and centrifuged at 4000 for 2 minute (Precellys Isolation of RNA: Isolated RNA by kit (Viral RNA isolation kit, Macherey- Nagel) Detection of antibody: We used recombinant ELISA kit to detection of antibody for NP protein of PPR which was manufactured b ID. Vet France (ID Screen PPR Competition. Code: PPRC, Batch: 0814) Detection of antigen : We used ELISA kit which was manufactured b ID. Vet France (ID Screen PPR sandwich. Code: PPRAg, Ver: 0614)

16 LABORATORY DIAGNOSIS The method of PPR diagnosis RT-PCR: We detected NP gene for PPR using NP3, 5 TCT CGG AAA TCG CCT CAC AGA CTG 3, NP4, 5 CCT CCT CCT GGT CCT CCA GAA TCT 3 primers. Real time PCR: We performed at Laboratory of OIE and international atomic energy agency (IAEA) in Vena city of Austria. (used itaq TM Universal Probe One-Step (Bio-Rad)

17 LABORATORY DIAGNOSIS Necropsy and clinical symptom Molecule biology Sero surveillance Ta take sample and necropsy New serum without any precipitation To isolate RNA and DNA To detect of antibody by ELISA Real time PCR, RT-PCR To detect antigen by ELISA Sequence Result Notified VABA After take a samples, to give a result in 3-6 hours.

18 LABORATORY DIAGNOSIS Use of in-field confirmatory diagnostic lateral flow device (BDSL) for PPR

19 LABORATORY DIAGNOSIS Result of qrt-pcr All samples taken by suspected animals for PPR. Дээжийн дараалал: Ховд аймаг, Дөргөн сум, ишигний эмгэгт эд. 1 Залгиур 21.72, 2 Нарийн гэдэс 17.79, 3 Элэг 27.76, 4 Уушги N/A, 5 Зүрх 26.93, 6 Бөөр N/A, 7 Дэлүү 19.3, 8 Чацга 22.2, 9 Нуух N/A, 10 Эерэг хяналт (Standard Pos RNA, IAEA, Bharani Kumar, ), 11 Эерэг хяналт (Мянгад сум ), 12 Эерэг хяналт (Годрон MCCP, Texas Red), 13 Сөрөг хяналт N/A, үлэмж цэвэр ус

20 LABORATORY DIAGNOSIS Result of RT-PCR From 12 samples which were suspected for PPR. 6 samples were positive by RT-PCR with PPR E gene specific primers. Samples: 1-2 negative control, 3-7 liver, kidney, lung, spleen and blood of goat from Buyant sum of Khovd aimag eye discharge, nasal discharge of goat from Myangat sum of Khovd aimag liver and hearth of goat grom Dugun sum of Khovd aimag Positive control (Standard Pos RNA, IAEA, Bharani Kumar, ).

21 LABORATORY DIAGNOSIS LABORATORY RESULT OF SAIGA ANTILOPE Test results No Origin Date Animal species Specimen PCR Ag ELISA Ref 1 Lung Saiga tatarica Spleen Khovd 27 December, 2016 mongolica 3 Eye discharge Saiga tatarica Spleen Gobi-Altai 11 January, mongolica Lung Lung Spleen Saiga tatarica Eye discharge + 8 Khovd 16 January, 2017 mongolica 9 Nasal discharge Tongue ephitelial + 10 Total 10 samples

22 LABORATORY DIAGNOSIS Phylogenic tree of Saiga.

23 COMMENTS A national (& possibly regional) emergency disease situation exists and is likely to escalate if not immediately contained. Local people are concerned for wildlife & feel livelihoods threatened and are committed to help practically and politically Food resources in the saiga range are limited by desert conditions & severe winters, competition from livestock, animals under stress, clearly shown by poor condition of animals which may have increased susceptibility to diseases Inadequate funding & lack of trained human resource for wildlife disease outbreak disease investigation and epidemiological monitoring, livestock seromonitoring (vaccine effectiveness) and surveillance, inadequate veterinary capacity for virus elimination but State Central Veterinary Laboratory is competent for diagnosis with upgraded facilities Science based inter-disciplinary, multi-sectoral decision making is vital, Maintain emergency request international/national funding, initiate TCP (support FAO/OIE secretariat mission) Inform international wildlife/saiga conservation organizations on Saiga/wildlife mortality situation for rapid support and response, Plan for spring animal movements, maintain restrictions on livestock use of saiga range to reduce stress & pathogen risk, initiate wildlife habitat protection measures & policies, Use this opportunity to kick-start PPR Global Eradication Program, safeguard livestock and ruminant biodiversity & associated livelihoods, One Health approach Focus on live not dead animals as the former transmit the infection not the carcasses (no threat to humans & low risk to livestock or wildlife), Initiate vaccination of livestock lambs/kids by July & adults if seromonitoring dictates, Current focus & spending on disposal & disinfection excessive whilst investment in monitoring of epidemic & planning for spring inadequate

24 COMMENTS Current livestock & wildlife epidemic situation under-reported but confirmed and more extensive than widely thought with still active infection indicated by wildlife outbreak & sporadic cases reported in livestock A national (& possibly regional) emergency disease situation exists and is likely to escalate if not immediately contained. Local people are concerned for wildlife & feel livelihoods threatened and are committed to help practically and politically Globally significant mass mortality event, endangered saiga antelope being driven to the edge of extinction & other species both domestic & wildlife also affected & tourism economy threatened. Note Kazakh population devastated in Food resources in the saiga range are limited by desert conditions & severe winters, competition from livestock, animals under stress, clearly shown by poor condition of animals which may have increased susceptibility to diseases Inadequate funding & lack of trained human resource for wildlife disease outbreak disease investigation and epidemiological monitoring, livestock seromonitoring (vaccine effectiveness) and surveillance, inadequate veterinary capacity for virus elimination but State Central Veterinary Laboratory is competent for diagnosis with upgraded facilities

25 Supporters of saiga disease research

26 THANK YOU FOR YOUR ATTENTION