1. Babesia bigemina. 2. Anaplasma marginale. 3. Theileria orientalis. 4. Trypanosoma evansi. Vector: Rhipicephalus (Boophilus) microplus.
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2 1. Babesia bigemina. Vector: Rhipicephalus (Boophilus) microplus. 2. Anaplasma marginale. Vector: Rhipicephalus (Boophilus) microplus. 3. Theileria orientalis. Vector: Rhipicephalus (Boophilus) microplus. Haemaphysalis bispinosa. 4. Trypanosoma evansi. Vector: Tabanus fly.
3 Predisposing factors associated with Theileria orientalis. Pregnancy (Last trimester). Peak lactation in case of high yielders. Newly introduced animal. Transportation stress. Pure and cross-bred animals. Concurrent viral and bacterial infections.
4 High rise of body temperature. Inappetance. Sudden drop in milk yield. Lacrymation. Anaemia. Abortion. Passing of tarry colored (Black) sticky dung. Non responsive to treatment with antibiotics and antiprotozoals used against babesiosis. Chronic cases will have normal body temperature but animal will be depressed and reluctance to eat.
5 Sample to be collected: Whole blood in EDTA/ Heparin. Diagnosis methods: Microscopic examination of Giemsa stained blood smear. Molecular detection by Polymerase Chain Reaction (PCR).
6 Animals can be treated if the case is detected early. Only drug of choice: Buparvaquone (Marketed as Zubion, Thelzon etc. by various brands). Presentation: 2 ml and 20 ml glass vials. Dosage: 2.5 mg per Kg body weight/ 1 ml per 20 kg body weight. Route: Deep intramuscular only. Schedule: Single injection is sufficient to cure the animals but may be repeated at 72 hours interval if parasite is again detected at 72 hours post treatment.
7 Screening of herd at least twice a year. Screening of the new animal for presence/absence of the parasite before introducing into the herd. Monitoring the pregnant animals, specially in their last trimester for signs of disease. Control of tick vectors by using appropriate acaricides in rotational manner at least thrice a year. Burning of unused hay and dried grass, cracks and crevices of the barn. Providing antistress/ immune-stimulant medicines like restobal, stressvit etc during pregnancy and after calving. No vaccines are available against Theileria orientalis. Owners should not use Rakshavac-T vaccine which is used aginst Theileria annulata and is not prevalent in this part of the country till now.
8 Anaplasma marginale Anaplasma marginale
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10 Babesia bigemina
11 Theileria orientalis
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15 LANE A B C D E F G 1000 bp 700 bp 500 bp 776 bp product of T.orientalis 100 bp Lane A: 100 bp Ladder Lane D,E,F : Positive Sample Lane B: Positive Control Lane G: Negative Sample Lane C: Negative Control
16 Lane A B C D E F G 800 bp 825 Bp product of T. orientalis(ikeda) 500 bp 100 bp Lane A: 100 bp Ladder Lane E,F,G :Negative Samples Lane B,C,D : 825 bp product of Theileria orientalis Ikeda Strain
17 LANE A B C D E F 500 bp 200 bp 100 bp 160 bp product of Anaplasma marginale Lane A: 100 bp Ladder Lane C,D : Positive Samples Lane B : Positive Control Lane E: Negative Control Lane F:Negative Sample
18 LANE A B C D E F G H 1100 bp 1000 bp 1124 bp product of Babesia bigemina 500 bp 100 bp Lane A: 1 kb Ladder Lane B: Positive Control Lane C: Negative Control Lane D,E,F,G : Positive Samples Lane H: Negative Samples
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27 Dr. S.K Das, PI cum HOD, DBT-NER-ADMaC, Dept. Of Microbiology, CVSc, AAU, Khanapara, Guwahati , Assam ; Dr. Parikshit Kakati, JRF cum PhD Scholar, DBT-NER-ADMaC, Dept. Of Parasitology, CVSc, AAU, Khanapara, Guwahati , Assam , Dr. Prabhat Chandra Sarmah, HOD, Department of Parasitology, CVSc, AAU, Khanapara, Guwahati: , Assam
both are fatal diseases. In babesiosis blood comes out with the urine and hence it is also known as Red water disease. Theileria vaccines are not
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