Survey Results for Method Needs
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1 Survey Results for Method Needs Kristi McCallum, CO Louise Ogden, MN {LM} Sharon Webb, UK-DRS 2017 Annual Summer AAFCO Laboratory Methods and Services Committee
2 Why and When? Backlog of methods needs when looking at the website Consolidation of our Notes & the website ID resources LMS Members, AGLabs Listserve, Compendium Opportunity for funding Look at antibiotics that are no longer in the marketplace Prioritize the remaining Survey of state labs Summer Annual 2017
3 How? Via conference call Kristi researched the drugs in marketplace in Colorado Compiled the list and reckoned with the Compendium for allowed uses That s where we started Wanted a survey: Short Concise To the point Not take too long sent to Aglabs 03/23/17 Final reminder via sent to Aglabs 03/29/17
4 Overview of Survey 5 questions Rank the methods Methods not listed Instrumentation you have or will have or will never have Check the drugs you are asked to run State or province 36 responses representing 2 Canadian Provinces & 21 states
5 Question 1: Prioritize the Method for Validation Multi-element, ICP-OES, ICP-MS Mycotoxin, LC/MS/MS Monensin, LC/MS/MS CTC & OTC, HPLC Tylosin, HPLC Multi-Drug Residue, LC/MS/MS Vitamin A, HPLC Vitamins (Fat Soluble), HPLC Vitamins (Water Soluble), HPLC Multi-residue Pesticides, LC/MS/MS Amino Acids (Lysine, Methionine, Taurine),
6 Vitamins (Water Soluble), HPLC 12.3 Vitamins (Fat Soluble), HPLC 11.1 Amino Acids (Lysine, Methionine, Taurine), UPLC, HPLC 9.6 Multi-residue Pesticides, LC/MS/MS 7.5 Tylosin, HPLC 7.4 Monensin, LC/MS/MS 7.2 Mycotoxin, LC/MS/MS 6.4 CTC & OTC, HPLC 5.9 Vitamin A, HPLC 5.9 Multi-Drug Residue, LC/MS/MS 5.7 Multi-element, ICP-OES, ICP-MS 4.1
7 Question 2: Please list any missing methods from list Crude Fat. Crude Fiber, Acid Hydrolysis Fats, Vitamin A Amprolium and Carbadox by HPLC Glyphosate in vegetation and Food Mono and Di-saccharides, Fructans, Carboadox and Pyrantel Tartrate by LC GC-MS/MS Multiple Pesticides Multi-Drug Formulation Level
8 Question 3: Instrumentation in my lab Have Answer Options Yes No Response Count HPLC LC-MS LC-MS/MS UPLC ICP-AES ICP-MS ICP-OES
9 Question 3: Instrumentation for my lab Answer Options Planning on Purchasing Within next 6 months Within next year Response Count HPLC LC-MS LC-MS/MS UPLC ICP-AES ICP-MS ICP-OES 0 2 2
10 Question 3: Instrumentation for my lab Do not have Answer Options No plans to purchase No funding Response Count HPLC LC-MS LC-MS/MS UPLC ICP-AES ICP-MS ICP-OES 2 2 4
11 Question 3: Comments AA, IC, GC, GC/MS GC, GC-MS, IC GCMS, GC MSMS, GC Various detectors, Spectrophotometer Combustion Analyzer fluorometer GC-MS, GC-MS/MS GC/MS, AA, Combustion, UV, Microwave 2 GC (variety of detectors) We also have a postcolumn derivatizer, uv, fluorescence, vis detectors for HPLC/UPLC I'm not sure why ICP-AES and ICP-OES are differentiated since they're the same thing?
12 Amprolium Bacitracin Zinc Carbadox Chlortetracycline/P Clopidol Decoquinate Diflubenzuron Florfenicol Laidlomycin Lincomycin Monensin Narasin Neomycin/Oxytetr Oxytetracycline Pyrantel Tartrate Ractopamine (S)-Methoprene Semduramicin Sulfadimethoxine Tilmicosin Tylosin/Sulfameth Zilpaterol Our laboratory Question 4: Check each drug you are asked to analyze (from 2016 Compendium) 80.0% 70.0% 60.0% 50.0% 40.0% 30.0% 20.0% 10.0% 0.0%
13 1 Lasalocid 2 Monensin 3 Chlortetracycline 4 Amprolium 5 Decoquinate 6 Tylosin 7 Oxytetracycline 8 CTC/Sulfamethazine 9 Carbadox 10 CTC/Penicillin/Sulfamethazine 11 Lincomycin 12 Salinomycin 13 Tylosin/Sulfamethazine 14 Narasin 15 (S)-Methoprene 16 Tiamulin 17 Virginiamycin 18 N/A 19 BMD 20 Diflubenzuron 21 Narasin/Nicarbazin 22 Tilmicosin 23 Bacitracin Zinc 24 Neomycin/OTC 25 Nicarbazin 26 Pyrantel Tartrate 27 Ractopamine 28 Sulfadimethoxine & Ormetoprim 5:1
14 29 Other (please specify) 30 Fenbendazole 31 Semduramicin 32 Clopidol 33 Cyromazine 34 Hygromycin B 35 Melengestrol Acetate 36 Robenidine Hydrochloride Sulfadimethoxine and Ormetoprim 37 5:3 38 Zoalene 39 Bambermycins 40 Diclazuril 41 Florfenicol 42 Laidlomycin 43 Morantel Tartrate 44 Poloxalene 45 Rabon 46 Zilpaterol Hydrochloride maduramicin, novobiocin, erythromycin Ivermectin, nitrofurazone, sulfamethazine, sulfathiazole,tetracycline HCl sufamethazine
15 Question 5: State/Province CN 2 CO 2 FL 1 IA 1 KY 2 LA 1 MD 1 MO 2 MN 2 MT 2 NC 1 NE 1 NV 1 NM 1 OH 1 OK 2 OR 2 PA 1 SC 1 SD 2 VA 2 WA
16 Next Steps
17 Thanks Louise Ogden, LM, RT, MN Kristi McCallum, CO Participants
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