RESEARCH ADVISORY COUNCIL APPLICATION FOR USE OF ANIMALS IN RESEARCH OR TRAINING
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1 (ssigned by ) R # RESERH DVISORY OUNIL PPLITION FOR USE OF NIMLS IN RESERH OR TRINING Please contact the omparative Medicine Department (ext# 31795/77672/24780) for assistance in completing this form. The form can be downloaded from site. The completed application should be submitted to the Office of Research ffairs () together with the research proposal or request to use animals for training purposes. Use additional pages if more space is required. 1. Proposal Title: Duration of the study: 2. Rationale, Goals, and nimal Research Methodologies: Please describe briefly but accurately the methodology of experiments involving animals. 3. Species and Number of nimals to be used: Please list all species to be used for the project (according to the standard nomenclature ( www. Informatics.jax.org/mgihome/nomen/). For each species indicate the strain, age, sex, weight, and the number you estimate will be needed for each years and for the entire project. Species Strain ge Sex Weight No. per years 1st 2nd 3rd No. Duration 4. Please explain why it is necessary to use animals, why the particular requested species are needed, and how the number of animals was calculated. ddress issues of statistical analysis, data variability, and sample size calculations pplicuse nimals_res ortraining revised 29jan12 per U minute dated 23/1/1433 (18/12/11)
2 (ssigned by ) R # 5. Location: Where will the animals be housed? Where will the animal studies be conducted? 6. Will this project involve the use of any of hazardous material? YES If YES, please complete the Biological, hemical & Radiological Hazards Form (Downloaded from site) 7. Will any of these animals be used in more than one project? YES If YES, please indicate the R number(s) of the other project(s) and explain why. 8. Pain and Distress: Discuss any aspect of the research project that has the potential to produce pain. Please grade pain/distress according to U guidelines (Quick Flow hart). Indicate your plan for reducing pain or distress through use of sedatives, anesthetics, or analgesics. [Note: if anesthesia is required then, by definition, there is the potential for pain or distress]. Type of Medication Dose Route of dministration Frequency 9. a) nimal Experiment Endpoint: learly state what is the endpoint of the experimental animal use {for example, aging research, cancer growth, etc} b) Euthanasia: Describe the method of euthanasia. If this requires administration of a drug, specify the dosage and route of administration. The method of euthanasia must be in accordance with the VM guidelines on Euthanasia ( unless the investigator provides written justification for any deviation based on scientific reasons. If animals will not be euthanized, what will happen to them? pplicuse nimals_res ortraining revised 29jan12 per U minute dated 23/1/1433 (18/12/11)
3 (ssigned by ) R # 10. lternatives to Use of nimals or Procedures Involving Pain or Distress: (a) re there alternative studies, procedures, or methods that could be conducted to answer the question of interest which would eliminate the need to use animals or reduce the number of animals needed? YES, please explain why these alternatives are not being employed. (b) side from euthanasia itself, will any of these animals require anesthesia or be subjected to any procedures which have the potential to produce more than slight momentary pain or distress? YES, please explain. (c) re there alternative studies, procedures, or methods that could be conducted which would lessen the pain or distress experienced by these animals? YES, please explain why these alternatives are not being employed. (d) Do these studies duplicate prior experimentation conducted by you or by others? YES, please explain why the studies are still needed. (e) The R regulations require that you indicate the information sources you consulted to develop the rationale and methods for this study, to ensure that these studies do not duplicate previous studies and to ensure that there were no alternatives that would reduce the use of animals or lessen the amount of pain or distress they might suffer. Indicate which information sources were searched, when the search was last done, and what keywords were used for database searches. Sources might include: the nimal Welfare Information enter, the urrent Research Information Service, Medline, Index Medicus, urrent ontents, specific journals, etc. Date of Most Recent Search Sources Searched Keywords Used 11. Description for the Laymen: a) Potential Benefits to Society: Indicate in laymen s terms the potential benefits to society that may be derived from this research. Use plain English and avoid using scientific jargon and abbreviations. b) Pain/Distress: Describe in laymen s terms the degree of pain or distress the animals are likely to experience by relating it as best you can to commonly understood medical, dental, or veterinary procedures pplicuse nimals_res ortraining revised 29jan12 per U minute dated 23/1/1433 (18/12/11)
4 (ssigned by ) R # omparative Medicine Budget / learance Form 1. nimal Protocols and Flow hart: Please describe the step by step protocol for all aspects of the project that involves the use of animals from receipt until euthanasia. This should include experimental groups, dministration of any drugs or special diet, and all surgery or other procedures to be performed on the animals. In addition, provide a flow chart (here or on a separate sheet) which summarizes the protocols using animals. 2. Requested Support: Beyond routine care of animals, are there any special space and/or caging, special services, or support which are requested to carry on the project (e.g. veterinary assistance, help with anesthesia, equipment, monitoring, GLP Quality ssurance, etc.?) YES, please describe. 3. nimal Budget: To be completed by omparative Medicine Department in oordination with the Principal Investigator (PI) nimal Facility osts Items: Numbers/time (hrs) ost/item/hour Subtotal nimal ost: nimal (#) Per diem (#) Special are & Housing (#) Transportation (#) nesthetics & Supplies: nesthesia supplies (#) nesthetic agents (#) nesthetist time (hr) Medical: Drugs (#) Supplies (#) pplicuse nimals_res ortraining revised 29jan12 per U minute dated 23/1/1433 (18/12/11)
5 (ssigned by ) R # nimal Facility osts ontinue from above Items: Numbers/time (hrs) ost/item/hour Subtotal Laboratory: Blood hemistry (#) Hematology (#) Serology (#) Supplies (#) Urine nalysis (#) Histology (#) Miscellaneous: Radiology/Imaging: X-ray (#) Echocardiogram (#) -arm (#) Xenogen (#) Surgical: Instrument trays (#) Miscellaneous (#) Nurses time (hr) Sterile supplies (#) Suture materials (#) Theatre use (hr) Post operative care (hr) Syringes & Needles (#) Technical nimal are: ollection of specimens (#) dministration of (#) drugs/biologicals Technicians time (hr) Veterinary onsultation (hr) Grand SR: hairman, omparative Medicine Department Signature Date pplicuse nimals_res ortraining revised 29jan12 per U minute dated 23/1/1433 (18/12/11)
6 (ssigned by ) R # Survival Surgery/Procedure Form Important Notes: The regulations on the use of laboratory animals require specific documentation for all survival surgical procedures. ppropriate forms can be obtained from the omparative Medicine Department (ext# 31795/77672/24780). Some forms must be completed and returned prior to surgery while others are to be completed intraoperatively and after the recovery period. ll forms must be returned to the veterinarians. Please reserve the survival surgery suite well in advance and discuss any requests for support with the veterinarians. re any animals to remain alive for any period of time after a surgical procedure? YES If YES, please complete the survival surgery/procedure form: 1 Surgical Team: List members of the surgical team, including surgeon(s), technicians, and anesthesiologist. Indicate their training and experience with the species being used. 2 Preoperative Preparation and are: Describe pre-operative care and preparations, including physical examination, lab tests, antibiotics, etc. 3 Surgical Procedure Description: 4 nesthesia Plan: Describe your anesthesia/analgesia plan, including premedication, intraoperative anesthesia, and paralytics. List dosages, frequency and route of administration. State what criteria you will use to assess the level of anesthesia during surgery. 5. Postoperative nalgesia Plan: Generally speaking, it is felt that major procedures require administration of analgesics for a certain period of time postoperatively. Describe your plan for evaluating and managing postoperative pain or distress. Be specific in describing how you will evaluate pain and the frequency with which assessment will take place. What drugs will be used for management of pain or distress? Give the dosage, route of administration, and frequency of administration. 6. Postoperative Recovery and are: Describe the postoperative recovery plan. Describe where recovery will take place, the personnel involved, how the animal will be monitored, and the specific criteria used to determine when the animal can safely be left unattended. Principal Investigator Signature Date pplicuse nimals_res ortraining revised 29jan12 per U minute dated 23/1/1433 (18/12/11)
7 (ssigned by ) R # pplicuse nimals_res ortraining revised 29jan12 per U minute dated 23/1/1433 (18/12/11)
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