ANTIBIOTIC SUSCEPTIBILITY TESTING BY THE CDS METHOD. Tables Only. Online Edition September S.M. Bell, B.J. Gatus, J.N. Pham, G.T.
|
|
- Jasper Cunningham
- 5 years ago
- Views:
Transcription
1 ANTIBIOTIC SUSCEPTIBILITY TESTING BY THE CDS METHOD Tles Only Online Edition Septemer 2009 S.M. Bell, B.J. Gtus, J.N. Phm, G.T. Fisher The Antiiotic Reference Lortory Deprtment of Microiology The Prince of Wles Hospitl South Estern Are Lortory Services Rndwick NSW 2031 Austrli Tel: (02) /9053 Fx: (02) E-mil: Wesite: South Estern Are Lortory Services Online Edition Septemer
2 Contents Contents CONTENTS...2 TABLES TABLES...3 A GUIDE TO THE USE OF THE TABLES CALIBRATIONS SURROGATE DISCS QUALITY ASSURANCE TESTING AND REPORTING Β-LACTAM ANTIBIOTICS...21 Tles Tle Clirtions: Grm Positive Orgnisms 4 Tle Clirtions: Grm Negtive Orgnisms 7 Tle 10.1.c. Clirtions: Aneroic Orgnisms. 11 Tle 10.1.d. Clirtions: Yest. 11 Tle Surrogte disc testing: Grm Positive Orgnisms 12 Tle Surrogte disc testing: Grm Negtive Orgnisms 14 Tle Reference strins: Grm Positive Orgnisms 16 Tle Reference strins: Grm Negtive Orgnisms 17 Tle 10.3.c. Reference strins: Aneroic Orgnisms 20 Tle 10.3.d. Reference strins: Yest 20 Tle A guide to the testing nd reporting of β-lctm ntiiotics for Grmnegtive orgnisms. 21 Online Edition Septemer
3 Chpter 10. Tles 10. Tles A Guide to the Use of the Tles There re 4 sets of tles contining informtion essentil for the performnce of the CDS Test. These tles re intended for oth Clinicl nd Veterinry lortories. Antiiotics used in veterinry medicine only re flgged. Clirtion tles 1, 1, 1c, & 1d collectively summrise much of the sic informtion used in the CDS Test. They list ll the orgnisms nd ntimicroils tested y the method, the medi used, conditions of incution, disc potencies, cut off sizes for unusul nnulr rdii nd the MICs for susceptile strins (rekpoints). The tles re updted regulrly nd opertors should ensure tht they re using the ltest versions of these tles. It is most importnt to py prticulr ttention to the footnotes included with ech tle s these highlight exceptions, restrictions nd some specific directions. For orgnisms not included in the Tle of Clirtions, extrpolte the testing from tht estlished for similr orgnisms. Surrogte Disc Testing tles 2 nd 2, list those ntimicroils where the susceptiility cn e inferred from the results otined with closely relted gent, the surrogte disc. The tle is rrnged ccording to cteril species nd the reltionship etween the ntimicroils is vlid only for the species indicted. This tle is lso updted regulrly s dt re ccumulted which either invlidte the reltionship or enle us to dd gents to the list. Lortories my find it useful to include comment on the susceptiility report tht the result reported with prticulr ntimicroil indictes the susceptiility to nother. Qulity ssurnce tles 3, 3, 3c & 3d list the 13 reference strins, the medi, the incution conditions of testing nd the expected rnge of zone sizes oserved with ech disc of stted potency. The footnotes explin how the cceptle rnges of zone sizes were derived nd recommend the indictions for nd the frequency of testing the reference strins. Tle 4 is guide through the mze of testing nd reporting the susceptiility of Grm-negtive eroic species to β-lctm ntiiotics. The production of one or more β-lctmses is n importnt nd common mechnism of resistnce in these species, ut for severl resons, resistnce my e difficult or impossile to demonstrte y the usul methods of ntiiotic susceptiility testing. The tle sets out, in some detil, those species where resistnce should e ssumed on the sis of previous documenttion of the presence of stle mechnism of resistnce nd those where susceptiility cn e relily demonstrted y the disc test. This tle lso relies hevily on the footnotes to drw ttention to exceptions nd specil circumstnces. Online Edition Septemer
4 Chpter 10. Tles Clirtions Tle Clirtions: Grm Positive Orgnisms Antiiotics, disc potencies, nnulr rdii nd MIC for susceptile strins, medi nd incution conditions. Disc potency (µg) Exception to the stndrd interprettion MIC (mg/l) Antiiotic Corynecterium species (Blood Sensitest, CO2, C) Ampicillin c 5 2 Benzylpenicillin 0.5 u Chlormphenicol 30 8 Ciprofloxcin Clindmycin n Erythromycin Fusidic cid Mrofloxcin k 5 2 Moxifloxcin/Gtifloxcin Quinupristin/Dlfopristin 15 2 Rifmpicin Teicoplnin 15 2 mm 8 Tetrcycline 10 4 Tigecycline 15 1 Vncomycin 5 2 mm 4 Enterococci (Blood Sensitest, CO 2, C) Ampicillin d 5 d 4 mm d 4 Chlormphenicol 30 4 mm 8 Gentmicin Linezolid 10 4 Mrofloxcin k 5 2 Nitrofurntoin e mm 64 Quinupristin/Dlfopristin f 15 2 Streptomycin mm 512 Teicoplnin 15 2 mm 8 Tigecycline 15 1 Vncomycin 5 (See foot note) g 4 Listeri species (Blood Sensitest, CO 2, C) Ampicillin 5 1 Co-trimoxzole /9.5 Gentmicin 10 1 The stndrd 6 mm cut-off pplies where no exception hs een specified. Slow growers re incuted for 48h. c If Corynecterium species is resistnt to enzylpenicillin 0.5 units, test mpicillin 5 µg. d Perform nitrocefin sed test to detect β lctmse ctivity if the zone of inhiition hs shrp edge nd n nnulr rdius > 4 mm. β Lctmse positive isoltes re reported s resistnt. e For testing urine isoltes only. f Enterococcus feclis is intrinsiclly resistnt to Pristinmycin nd Quinupristin/Dlfopristin. g A zone of inhiition with hzy edge indictes low level resistnce to Vncomycin (VnB type), irrespective of the size of the inhiitory zone. The hzy edge will e more evident if the plte reincuted for totl of 48. k Antiiotic clirted for veterinry medicine. n Isoltes with inducile clindmycin resistnce (imlsb phenotype) will hve lrge zone to clindmycin, ut should e reported s resistnt. Inducile clindmycin resistnce cn e detected s descried in section 4.8 of the CDS mnul. Online Edition Septemer
5 Chpter 10. Tles Tle Clirtions: Grm Positive Orgnisms cont. Antiiotics, disc potencies, nnulr rdii nd MIC for susceptile strins, medi nd incution conditions. Disc potency (µg) Exception to the stndrd interprettion MIC (mg/l) Antiiotic Nocdi species (Blood Sensitest, 5% Air, C) Amikcin 30 8 Ciprofloxcin Clrithromycin Co-trimoxzole 25 2/38 Gentmicin 10 2 Imipenem 10 4 Linezolid 10 2 Tigecycline 15 2 Stphylococci (Sensitest, Air C) Ampicillin h Benzylpenicillin i 0.5 u 0.06 Cefoxitin j 10 4 Cephlexin h Chlormphenicol 30 8 Ciprofloxcin Clindmycin n Co-trimoxzole 25 1/19 Erythromycin Fusidic cid Gentmicin 10 1 Knmycin 50 8 Linezolid 10 4 Mrofloxcin k 5 2 Moxifloxcin/Gtifloxcin Mupirocin 5 2 Neomycin k 30 4 mm 8 Nitrofurntoin e Novoiocin k 5 1 Oxcillin l Quinupristin/Dlfopristin 15 2 Rifmpicin Sulphfurzole Teicoplnin 15 2 mm 8 Tetrcycline 10 4 Tigecycline 15 1 Trimethoprim 5 4 Vncomycin 5 2 mm 4 The stndrd 6 mm cut-off pplies where no exception hs een specified. e For testing urine isoltes only. h For testing Stphylococcus sprophyticus only. i Not for testing Stphylococcus sprophyticus. j For testing Stphylococcus ureus only. k Antiiotic clirted for veterinry medicine. l For testing cogulse-negtive stphylococci (except Stphylococcus sprophyticus). n Isoltes with inducile clindmycin resistnce (imlsb phenotype) will hve lrge zone to clindmycin, ut should e reported s resistnt. Inducile clindmycin resistnce cn e detected s descried in section 4.8 of the CDS mnul. Online Edition Septemer
6 Chpter 10. Tles Tle Clirtions: Grm Positive Orgnisms cont. Antiiotics, disc potencies, nnulr rdii nd MIC for susceptile strins, medi nd incution conditions. Antiiotic Disc potency (µg) Exception to the stndrd interprettion MIC (mg/l) Streptococci & Erysipelothrix species (Blood Sensitest, CO 2, C) m Ampicillin m 5 4 mm 2 Benzylpenicillin 0.5 u Cefotxime Cefotxime m 5 2 Ceftrixone Ceftrixone m 5 2 Chlormphenicol 30 8 Clindmycin n Co-trimoxzole /9.5 Erythromycin Mrofloxcin k 5 2 Moxifloxcin/Gtifloxcin mm 1 Nitrofurntoin e Quinupristin/Dlfopristin 15 2 Rifmpicin Teicoplnin 15 2 mm 8 Tetrcycline 10 4 Tigecycline 15 1 Vncomycin 5 2 mm 4 The stndrd 6 mm cut-off pplies where no exception hs een specified. e For testing urine isoltes only. k Antiiotic clirted for veterinry medicine. m NOT for testing Streptococcus pneumonie from CSF. If Streptococcus pneumonie or ny other Streptococcus species from site other thn CSF is resistnt to enzylpenicillin 0.5 units, cefotxime 0.5 µg or ceftrixone 0.5 µg then test mpicillin 5 µg, cefotxime 5 µg nd ceftrixone 5 µg. n Isoltes with inducile clindmycin resistnce (imlsb phenotype) will hve lrge zone to clindmycin, ut should e reported s resistnt. Inducile clindmycin resistnce cn e detected s descried in section 4.8 of the CDS mnul. Online Edition Septemer
7 Chpter 10. Tles Tle Clirtions: Grm Negtive Orgnisms Antiiotics, disc potencies, nnulr rdii nd MIC for susceptile strins, medi nd incution conditions. Antiiotic Disc potency (µg) Exception to the stndrd interprettion MIC (mg/l) Acinetocter species, Enteroctericee & Virioncee (Sensitest, ir, C) Amikcin 30 4 mm c 16 c Ampicillin 25 8 Aprmicin d 15 8 Augmentin e 60 16/8 Aztreonm 30 8 Cefzolin Cefepime 10 2 Cefotxime 5 1 Cefotetn 30 8 Cefoxitin 30 8 Cefpirome 10 2 Cefpodoxime 10 2 Ceftzidime 10 4 Ceftrixone 5 1 Cefuroxime 30 8 Cephlexin Chlormphenicol 30 8 Ciprofloxcin Enoxcin 10 4 Ertpenem f 10 4 Gentmicin 10 4 mm 2 Imipenem 10 4 Knmycin 50 8 Mrofloxcin d 5 2 Meropenem 5 2 Moxifloxcin/Gtifloxcin Nlidixic cid g 30 4 Neomycin d 30 4 mm 8 Nitrofurntoin g Norfloxcin g 10 4 Polymyxin B 300 u 4 mm 1 Spectinomycin d Streptomycin d Sulphfurzole Tzocin e 55 16/2 Tetrcycline 10 4 mm 4.0 Tigecycline 15 1 Timentin e 85 32/2 Tormycin 10 4 mm 2 Trimethoprim 5 4 The stndrd 6 mm cut-off pplies where no exception hs een specified. Yersini enterocolitic is incuted in ir t 30 C. c Revised clirtion, d Antiiotic clirted for veterinry medicine. e If n ESBL is present, report Augmentin, Timentin nd Tzocin for isoltes from urine only. f Acinetocter species re considered resistnt to ertpenem. g For testing urinry isoltes only. Online Edition Septemer
8 Chpter 10. Tles Tle Clirtions: Grm Negtive Orgnisms cont. Antiiotics, disc potencies, nnulr rdii nd MIC for susceptile strins, medi nd incution conditions. Antiiotic Disc potency (µg) Exception to the stndrd interprettion MIC (mg/l) Brnhmell ctrrhlis (Morxell ctrrhlis) (Blood Sensitest, 5% CO2, C) Benzylpenicillin 0.5 u 0.25 Cefclor 30 4 Cefpodoxime 10 2 Cefuroxime 30 4 Chlormphenicol 10 4 Ciprofloxcin Co-trimoxzole 25 1/19 Erythromycin Mrofloxcin d 5 2 Moxifloxcin/Gtifloxcin Tetrcycline 10 4 Cmpylocter species (Blood Sensitest, microerophilic, 42 C) Ciprofloxcin Erythromycin 5 4 mm 0.5 Gentmicin 10 1 Tetrcycline 10 4 Hemophilus species (HTM gr h, 5% CO2, C) Ampicillin 5 1 Augmentin 15 2/1 Cefclor 30 4 Cefotxime Cefotxime 5 1 Cefpodoxime 10 2 Ceftrixone Ceftrixone 5 1 Cefuroxime 30 4 Chlormphenicol 10 2 Ciprofloxcin Co-trimoxzole 25 1/19 Mrofloxcin d 5 2 Moxifloxcin/Gtifloxcin Tetrcycline 10 4 Helicocter pylori (Chocolte Columi Blood Agr, microerophilic, C) Amoxycillin 2 1 Ciprofloxcin Erythromycin i Metronidzole 5 4 Rifmpicin j 5 2 Tetrcycline 10 4 The stndrd 6 mm cut-off pplies where no exception hs een specified. d Antiiotic clirted for veterinry medicine. h Hemophilus Test Medium Bse contining 15 mg/l of freshly prepred hemtin nd NAD. i Erythromycin 5 µg is the surrogte disc for reporting the susceptiility to clrithromycin. The MIC of clrithromycin for susceptile strins is 0.5 mg/l. j Rifmpicin 5 µg is the surrogte disc for reporting the susceptiility to rifutin. Online Edition Septemer
9 Chpter 10. Tles Tle Clirtions: Grm Negtive Orgnisms cont. Antiiotics, disc potencies, nnulr rdii nd MIC for susceptile strins, medi nd incution conditions. Antiiotic Disc potency (µg) Exception to the stndrd interprettion Neisseri gonorrhoee k (Chocolte Columi Blood Agr, 5% CO2, C, humidity > 80%) Benzylpenicillin 0.5 u MIC (mg/l) Susceptile k > l Less susceptile k l Resistnt k < 3 1 l Ceftrixone 0.5 Susceptile k > 9 < 0.03 l Non susceptile k l Resistnt k Ciprofloxcin/Nlidixic cid CIP 1 / NA 30 CIP 1 NA 30 Susceptile k > 6 > n Less susceptile k,m > 6 0 m n Resistnt k,m 6 0 m 1 n Spectinomycin 100 Susceptile k 6 64 Less susceptile k Resistnt k Neisseri meningitidis (Blood Sensitest, 5% CO2, C) Benzylpenicillin 0.5 u 4 mm 0.25 Cefotxime Ceftrixone Chlormphenicol 10 2 Ciprofloxcin Rifmpicin The stndrd 6 mm cut-off pplies where no exception hs een specified. k Neisseri gonorrhoee sensitivity interprettion: Susceptile A cure is expected following tretment with stndrd doses. Less susceptile Quinolone gents: A cure would e expected following high orl dose regimens e.g. 500 mg ciprofloxcin, ut tretment filure from lower doses. Penicillin Group: A cure would e expected with the stndrd regimens used to tret mle urethrl gonorrhoe (e.g. single orl dose of 3g moxycillin), ut tretment filure my occur for other infected sites e.g. endocervix, phrynx nd rectum. Non susceptile Reduced zone size to Ceftrixone. Clinicl significnce is currently uncertin. Resistnt There is high proility of tretment filure using current dose recommendtions. l MIC determined y douling dilution gr plte technique. m On rre occsions (< 0.001% of isoltes) smll zone to Nlidixic Acid my e oserved in ssocition with decresed ciprofloxcin zone sizes. If the findings re confirmed on repet testing the isolte should e reported s less susceptile or resistnt ccording to the ciprofloxcin zone size nd the isolte referred for MIC testing. n MICs for ciprofloxcin. Online Edition Septemer
10 Chpter 10. Tles Tle Clirtions: Grm Negtive Orgnisms cont. Antiiotics, disc potencies, nnulr rdii nd MIC for susceptile strins, medi nd incution conditions. Disc potency (µg) Exception to the stndrd interprettion MIC (mg/l) Antiiotic Psteurell species (Blood Sensitest, 5% CO2, C) Ampicillin o 5 2 Benzylpenicillin 0.5 u 4 mm 0.25 Ciprofloxcin Mrofloxcin d 5 2 Moxifloxcin/Gtifloxcin Tetrcycline 10 4 Pseudomons species, Burkholderi species & Chryseocterium species (Sensitest, ir, C) Amikcin 30 4 mm 16 Augmentin p 60 8/4 Aztreonm 30 8 Cefepime 10 2 Cefpirome 10 2 Ceftzidime 10 4 Ciprofloxcin Ertpenem 10 4 Gentmicin 10 4 mm 4 Imipenem 10 4 Mrofloxcin d 5 2 Meropenem 5 2 Moxifloxcin/Gtifloxcin Norfloxcin g 10 4 Pipercillin Polymyxin B 300 u 4 mm 1 Sulphfurzole Tzocin 55 16/2 Ticrcillin Timentin 85 32/2 Tormycin 10 4 mm 4 Trimethoprim 5 4 Stenotrophomons mltophili q (Sensitest, ir, C) Sulphfurzole q The stndrd 6 mm cut-off pplies where no exception hs een specified. d Antiiotic clirted for veterinry medicine. g For testing urine isoltes only o Psteurell multocid is tested ginst mpicillin 5 µg nd NOT enzylpenicillin 0.5 units. p Burkholderi pseudomllei is usully susceptile to Augmentin nd cn e tested ginst this ntiiotic. q See Section 5.11 of the CDS mnul for notes on testing ntiiotic susceptiilities of Sulphfurzole resistnt S. mltophili. Online Edition Septemer
11 Chpter 10. Tles Tle 10.1.c. Clirtions: Aneroic Orgnisms. Antiiotics, disc potencies, nnulr rdii nd MIC for susceptile strins, medi nd incution conditions. Disc potency (µg) Exception to the stndrd interprettion MIC (mg/l) Antiiotic Aneroic orgnisms (Supplemented Brucell Medium Bse, neroic, C) c Augmentin 3 4 mm 1/2 Benzylpenicillin 0.5 u 0.25 Cefoxitin 10 4 Clindmycin 2 4 mm 0.5 Meropenem 5 2 Metronidzole 5 4 mm 2 Moxifloxcin mm 2 Tzocin 55 8/2 Timentin 85 32/2 The stndrd 6 mm cut-off pplies where no exception hs een specified. c Slow growers should e incuted for 48 hrs. Brucell Medium Bse contining 5% defirinted horse lood, hemin 5 mg/l nd vitmin K 1 mg/l. Tle 10.1.d. Clirtions: Yest. Antiiotics, disc potencies, nnulr rdii nd MIC for susceptile strins, medi nd incution conditions. Disc potency (µg) Exception to the stndrd interprettion MIC (mg/l) Antiiotic Yests (Csitone complex medium, ir, 30 C) Amphotericin B 10 4 mm Cspofungin 5 2 mm 2.0 Fluconzole 25 6 mm 16 Itrconzole 10 4 mm 1.0 Voriconzole 1 6 mm 1.0 The stndrd 6 mm cut-off pplies where no exception hs een specified. Online Edition Septemer
12 Chpter 10. Tles Surrogte Discs Tle Surrogte disc testing: Grm Positive Orgnisms Antiiotics tht cn e reported sed on susceptiility results otined with surrogte disc. Antiiotic reported Surrogte disc used Disc potency (µg) Corynecterium species Amoxycillin/Ampicillin/Penicillin V Benzylpenicillin 0.5 u Azithromycin/Clrithromycin/Roxithromycin Erythromycin 5 Cefovecin /Ceftiofur /other Cephlosporins Benzylpenicillin 0.5 u Enrofloxcin /Orifloxcin Moxifloxcin 2.5 Lincomycin Clindmycin 2 Norfloxcin c /Ofloxcin Ciprofloxcin 2.5 Pristinmycin Quinupristin/Dlfopristin 15 Tetrcyclines Tetrcycline 10 Tylosin Erythromycin 5 Enterococci Amoxycillin/Benzylpenicillin Ampicillin 5 Pristinmycin f Quinupristin/Dlfopristin 15 Listeri species Amoxycillin/Benzylpenicillin Ampicillin 5 Stphylococci (except S. sprophyticus from urine) Amoxycillin/Ampicillin/Penicillin V Benzylpenicillin 0.5 u Augmentin Oxcillin d /Cefoxitin e 1 d / 10 e Azithromycin/Clrithromycin/Roxithromycin Erythromycin 5 Cefovecin /Ceftiofur /other Cephlosporins Oxcillin d /Cefoxitin e 1 d / 10 e Cloxcillin/ Dicloxcillin/ Flucloxcillin Oxcillin d /Cefoxitin e 1 d / 10 e Enrofloxcin /Orifloxcin Moxifloxcin 2.5 Lincomycin Clindmycin 2 Norfloxcin c /Ofloxcin Ciprofloxcin 2.5 Pristinmycin Quinupristin/Dlfopristin 15 Sulphonmides Sulphfurzole 300 Tetrcyclines Tetrcycline 10 Tylosin Erythromycin 5 Stphylococcus sprophyticus from urine Amoxycillin/Benzylpenicillin/Penicillin V Ampicillin 5 Augmentin Cephlexin 100 Cefovecin /Ceftiofur /other Cephlosporins Cephlexin 100 Cloxcillin/Dicloxcillin/Flucloxcillin Cephlexin 100 Enrofloxcin /Orifloxcin Moxifloxcin 2.5 Norfloxcin c /Ofloxcin Ciprofloxcin 2.5 Sulphonmides Sulphfurzole 300 Tetrcyclines Tetrcycline 10 c d e f Antiiotic used in veterinry medicine only. Ceftzidime is inctive ginst Grm-positive orgnisms. Reporting of norfloxcin is for urine isoltes only. Oxcillin is used for testing cogulse-negtive stphylococci (except Stphylococcus sprophyticus) only. Cefoxitin is used for testing Stphylococcus ureus only. Enterococcus feclis is intrinsiclly resistnt to Pristinmycin nd Quinupristin/Dlfopristin. Online Edition Septemer
13 Chpter 10. Tles Tle Surrogte disc testing: Grm Positive Orgnisms cont. Antiiotics tht cn e reported sed on susceptiility results otined with surrogte disc. Antiiotic reported Surrogte disc used Disc potency (µg) Streptococci g Amoxycillin/Ampicillin/Penicillin V Benzylpenicillin 0.5 u Amoxycillin/Benzylpenicillin Ampicillin h 5 Azithromycin/Clrithromycin/Roxithromycin Erythromycin 5 Cefovecin /Ceftiofur Benzylpenicillin 0.5 u Cephlosporins (except ceftiofur) Cefotxime/Ceftrixone 0.5 Lincomycin Clindmycin 2 Pristinmycin Quinupristin/Dlfopristin 15 Tetrcyclines Tetrcycline 10 Tylosin Erythromycin 5 Antiiotic used in veterinry medicine only. g For streptococci groups A, B, C, G nd Streptococcus nginosus, the susceptiility to enzylpenicillin, mpicillin, moxycillin nd cephlosporin ntiiotics (except ceftzidime) is extrpolted from the testing of enzylpenicillin 0.5 units. h NOT for testing Streptococcus pneumonie from CSF. Test if isolte is resistnt to enzylpenicillin 0.5 units, cefotxime 0.5 mg or ceftrixone 0.5 mg. Online Edition Septemer
14 Chpter 10. Tles Tle Surrogte disc testing: Grm Negtive Orgnisms Antiiotics tht cn e reported sed on susceptiility results otined with surrogte disc. Antiiotic reported Surrogte disc used Disc potency (µg) Acinetocter, Enteroctericee & Virioncee species Amoxycillin Ampicillin 25 Cefovecin /Ceftiofur Cefzolin 30 Cephlothin Ampicillin 25 Ceftrixone Cefotxime 5 Cefotxime Ceftrixone 5 Co-trimoxzole c Sulphfurzole 300 Co-trimoxzole c Trimethoprim 5 Enrofloxcin /Orifloxcin Moxifloxcin 2.5 Ofloxcin Ciprofloxcin 2.5 Pipercillin Ampicillin 25 Sulphonmides Sulphfurzole 300 Tetrcyclines Tetrcycline 10 Ticrcillin Ampicillin 25 Brnhmell ctrrhlis (Morxell ctrrhlis) Azithromycin/Clrithromycin/Roxithromycin Erythromycin 5 Amoxycillin/Ampicillin/Penicillin V Benzylpenicillin 0.5 u Augmentin Cefuroxime/Cefclor 30 Cefovecin /Ceftiofur Cefuroxime/Cefclor 30 Cephlosporins Cefuroxime/Cefclor 30 Enrofloxcin /Orifloxcin Moxifloxcin 2.5 Ofloxcin Ciprofloxcin 2.5 Tetrcyclines Tetrcycline 10 Cmpylocter species Enrofloxcin /Orifloxcin Ciprofloxcin 2.5 Tetrcyclines Tetrcycline 10 Hemophilus influenze/hemophilus species Amoxycillin Ampicillin 5 Cefepime Cefotxime/Ceftrixone 0.5 Cefotxime Ceftrixone 0.5 Cefpirome Cefotxime/Ceftrixone 0.5 Ceftzidime Cefotxime/Ceftrixone 0.5 Cefovecin /Ceftiofur Cefuroxime/Cefclor 30 Ceftrixone Cefotxime 0.5 Cephlexin Cefuroxime/Cefclor 30 Enrofloxcin /Orifloxcin Moxifloxcin 2.5 Ofloxcin Ciprofloxcin 2.5 Tetrcyclines Tetrcycline 10 Helicocter pylori Clrithromycin Erythromycin 5 Rifutin Rifmpicin 5 c Antiiotic used in veterinry medicine only. Not for Acinetocter species. Resistnce to co-trimoxzole is indicted y resistnce to oth sulphfurzole nd trimethoprim. Online Edition Septemer
15 Chpter 10. Tles Tle Surrogte disc testing: Grm Negtive Orgnisms cont. Antiiotics tht cn e reported sed on susceptiility results otined with surrogte disc. Antiiotic reported Surrogte disc used Disc potency (µg) Neisseri meningitidis Ampicillin/Amoxycillin Benzylpenicillin 0.5 u Cefotxime Ceftrixone 0.5 Cefovecin /Ceftiofur Benzylpenicillin 0.5 u Ceftrixone Cefotxime 0.5 Enrofloxcin /Orifloxcin Moxifloxcin 2.5 Psteurell species Amoxycillin/Benzylpenicillin Ampicillin 5 Ampicillin/moxycillin Benzylpenicillin d 0.5 u Cefovecin /Ceftiofur Ampicillin 5 Enrofloxcin /Orifloxcin Moxifloxcin 2.5 Ofloxcin Ciprofloxcin 2.5 Tetrcyclines Tetrcycline 10 Pseudomons species, Burkholderi species & Chryseocterium species Azlocillin Pipercillin 50 Colistin Polymyxin B 300 u Co-trimoxzole c Trimethoprim 5 Co-trimoxzole c Sulphfurzole 300 Enrofloxcin /Orifloxcin Moxifloxcin 2.5 Norfloxcin c /Ofloxcin Ciprofloxcin 2.5 Stenotrophomons mltophili Co-trimoxzole Sulphfurzole 300 Antiiotic used in veterinry medicine only. c Resistnce to co-trimoxzole is indicted y resistnce to oth sulphfurzole nd trimethoprim. d Psteurell multocid is tested ginst mpicillin 5 µg nd NOT enzylpenicillin 0.5 units. Online Edition Septemer
16 Chpter 10. Tles Qulity Assurnce Tle Reference strins: Grm Positive Orgnisms Antiiotics, disc potencies nd cceptle zones of inhiition for reference strins. Antiiotic reported Disc potency (µg) Annulr rdii (mm) Enterococcus feclis ACM 5184 (Blood Sensitest, CO 2 35 C) Ampicillin Chlormphenicol Gentmicin Linezolid Nitrofurntoin Streptomycin Tigecycline Teicoplnin Vncomycin Stphylococcus ureus ACM 5190 (Sensitest, ir 35 C) Amoxycillin c Ampicillin Benzylpenicillin 0.5 u Cefoxitin Cephlexin Chlormphenicol Ciprofloxcin Clrithromycin Clindmycin Co-trimoxzole Erythromycin Fusidic cid Gtifloxcin Gentmicin Knmycin Linezolid Mrofloxcin d Moxifloxcin Mupirocin Neomycin d Nitrofurntoin Novoiocin d Oxcillin Quinupristin/Dlfopristin Rifmpicin Rifmpicin c Sulphfurzole Teicoplnin Tetrcycline Tigecycline Trimethoprim Vncomycin Reference strin testing must e performed: (i) In conjunction with the clinicl isolte, or t lest once weekly; (ii) When new tch of medium is used; (iii) When new tch of discs is used. The cceptle rnge (95% confidence limits) is the men ± 2 stndrd devitions. The men ws derived from > 120 mesurements with different opertors using different tches of oth gr nd discs. It is sttisticlly cceptle to use one hundred mesurements to represent the norml distriution nd this gives confidence limit of 95%, mening n in-uilt MU of 5% for the test. c The 2 µg moxycillin nd 5 µg Rifmpicin discs re used for the susceptiility testing of H. pylori only. d Antiiotic used in veterinry medicine only. Online Edition Septemer
17 Chpter 10. Tles Tle Reference strins: Grm Positive Orgnisms cont. Antiiotics, disc potencies nd cceptle zones of inhiition for reference strins. Antiiotic reported Disc potency (µg) Annulr rdii (mm) Streptococcus pneumonie ACM 5191 (Blood Sensitest, 5% CO 2, C) Ampicillin Benzylpenicillin 0.5 u Cefotxime Cefotxime Ceftrixone Ceftrixone Chlormphenicol Clindmycin Co-trimoxzole Erythromycin Gtifloxcin Moxifloxcin Quinupristin/Dlfopristin Rifmpicin Teicoplnin Tetrcycline Tigecycline Vncomycin Reference strin testing must e performed: (i) In conjunction with the clinicl isolte, or t lest once weekly; (ii) When new tch of medium is used; (iii) When new tch of discs is used. The cceptle rnge (95% confidence limits) is the men ± 2 stndrd devitions. The men ws derived from > 120 mesurements with different opertors using different tches of oth gr nd discs. It is sttisticlly cceptle to use one hundred mesurements to represent the norml distriution nd this gives confidence limit of 95%, mening n in-uilt MU of 5% for the test. Tle Reference strins: Grm Negtive Orgnisms Antiiotics, disc potencies nd cceptle zones of inhiition for reference strins. Antiiotic reported Disc potency (µg) Annulr rdii (mm) Bcteroides frgilis ACM 5196 c (Blood Sensitest, neroic, C) Metronidzole Cmpylocter jejuni ACM 5183 (Blood Sensitest, microerophilic, 42 C) Ciprofloxcin Erythromycin Gentmicin Tetrcycline Reference strin testing must e performed: (i) In conjunction with the clinicl isolte, or t lest once weekly; (ii) When new tch of medium is used; (iii) When new tch of discs is used. The cceptle rnge (95% confidence limits) is the men ± 2 stndrd devitions. The men ws derived from > 120 mesurements with different opertors using different tches of oth gr nd discs. It is sttisticlly cceptle to use one hundred mesurements to represent the norml distriution nd this gives confidence limit of 95%, mening n in-uilt MU of 5% for the test. c Bcteroides frgilis (ACM 5196) my e used s the reference strin when testing H. pylori ginst metronidzole. When testing neroic orgnisms, Clostridium perfringens (ACM 5240) should e used s the reference orgnism. Online Edition Septemer
18 Chpter 10. Tles Tle Reference strins: Grm Negtive Orgnisms cont. Antiiotics, disc potencies nd cceptle zones of inhiition for reference strins. Antiiotic reported Disc potency (µg) Annulr rdii (mm) Escherichi coli ACM 5185 d (Sensitest, ir, C) Amikcin Ampicillin Aprmicin d Aztreonm Cefzolin Cefepime Cefotxime Cefotetn Cefoxitin Cefpirome Cefpodoxime Ceftzidime Ceftrixone Cefuroxime Cephlexin Chlormphenicol Ciprofloxcin Gtifloxcin Enoxcin Ertpenem Gentmicin Imipenem Knmycin Mrofloxcin e Meropenem Moxifloxcin Nlidixic cid Neomycin e Nitrofurntoin Norfloxcin Polymyxin B 300 u Spectinomycin d Streptomycin e Sulphfurzole Tetrcycline Tigecycline Tormycin Trimethoprim Escherichi coli ACM 5186 (Sensitest, ir, C) Augmentin Timentin Tzocin Reference strin testing must e performed: (i) In conjunction with the clinicl isolte, or t lest once weekly; (ii) When new tch of medium is used; (iii) When new tch of discs is used. The cceptle rnge (95% confidence limits) is the men ± 2 stndrd devitions. The men ws derived from > 120 mesurements with different opertors using different tches of oth gr nd discs. It is sttisticlly cceptle to use one hundred mesurements to represent the norml distriution nd this gives confidence limit of 95%, mening n in-uilt MU of 5% for the test. d If ntiiotic discs re tested with Escherichi coli ACM 5185, there is no need to test these ginst Pseudomons eruginos ACM 5189 s well nd vice vers. e Antiiotic used in veterinry medicine only Online Edition Septemer
19 Chpter 10. Tles Tle Reference strins: Grm Negtive Orgnisms cont. Antiiotics, disc potencies nd cceptle zones of inhiition for reference strins. Antiiotic reported Disc potency (µg) Annulr rdii (mm) Hemophilus influenze ACM 5187 (HTM f gr, 5% CO 2, C) Ampicillin Cefclor Cefotxime Cefpodoxime Ceftrixone Cefuroxime Chlormphenicol Ciprofloxcin Co-trimoxzole Gtifloxcin Moxifloxcin Tetrcycline Hemophilus influenze ACM 5188 (HTM f gr, 5% CO 2, C) Augmentin Neisseri gonorrhoee ACM 5239 (WHO C) (Chocolte Columi Blood Agr, 5% CO 2, C, humidity > 80%) Benzylpenicillin 0. 5 u Ceftrixone Ciprofloxcin Nlidixic cid Spectinomycin Pseudomons eruginos ACM 5189 d (Sensitest, ir, C) Amikcin Aztreonm Cefepime Cefpirome Ceftzidime Ciprofloxcin Ertpenem g 10 g Gtifloxcin Gentmicin Imipenem Meropenem Moxifloxcin g 2.5 g Norfloxcin Pipercillin Polymyxin B 300 u Ticrcillin Tormycin Reference strin testing must e performed: (i) In conjunction with the clinicl isolte, or t lest once weekly; (ii) When new tch of medium is used; (iii) When new tch of discs is used. The cceptle rnge (95% confidence limits) is the men ± 2 stndrd devitions. The men ws derived from > 120 mesurements with different opertors using different tches of oth gr nd discs. It is sttisticlly cceptle to use one hundred mesurements to represent the norml distriution nd this gives confidence limit of 95%, mening n in-uilt MU of 5% for the test. d If ntiiotic discs re tested with Escherichi coli ACM 5185, there is no need to test these ginst Pseudomons eruginos ACM 5189 s well nd vice vers. f Hemophilus Test Medium Bse contining 15 mg/l freshly prepred Hemtin nd NAD. g Ertpenem nd Moxifloxcin should e tested ginst E. coli ACM Online Edition Septemer
20 Chpter 10. Tles Tle 10.3.c. Reference strins: Aneroic Orgnisms Antiiotics, disc potencies nd cceptle zones of inhiition for reference strins. Antiiotic reported Disc potency (µg) Annulr rdii (mm) Clostridium perfringens ACM 5240 (Supplemented Brucell Medium Bse, neroic, C) c Benzylpenicillin 0. 5 u Cefoxitin Clindmycin Meropenem Metronidzole Moxifloxcin Bcteroides frgilis ACM 5196 (Supplemented Brucell medium Bse, neroic, C) c Augmentin Tzocin Timentin Reference strin testing must e performed: (i) In conjunction with the clinicl isolte, or t lest once weekly; (ii) When new tch of medium is used; (iii) When new tch of discs is used. The cceptle rnge (95% confidence limits) is the men ± 2 stndrd devitions. The men ws derived from > 120 mesurements with different opertors using different tches of oth gr nd discs. It is sttisticlly cceptle to use one hundred mesurements to represent the norml distriution nd this gives confidence limit of 95%, mening n in-uilt MU of 5% for the test. c Brucell Medium Bse contining 5% defirinted horse lood, hemin 5 mg/l nd vitmin K 1 mg/l. Tle 10.3.d. Reference strins: Yest Antiiotics, disc potencies nd cceptle zones of inhiition for reference strins. Antiiotic reported Disc potency (µg) Annulr rdii (mm) Cndid prpsilosis ACM 5283 (Csitone Complex medium, eroic, 30 C) Amphotericin B Cspofungin Fluconzole Itrconzole Voriconzole Reference strin testing must e performed: (i) In conjunction with the clinicl isolte, or t lest once weekly; (ii) When new tch of medium is used; (iii) When new tch of discs is used. The cceptle rnge (95% confidence limits) is the men ± 2 stndrd devitions. The men ws derived from > 120 mesurements with different opertors using different tches of oth gr nd discs. It is sttisticlly cceptle to use one hundred mesurements to represent the norml distriution nd this gives confidence limit of 95%, mening n in-uilt MU of 5% for the test. Online Edition Septemer
21 Chpter 10. Tles Testing nd reporting β-lctm ntiiotics Tle A guide to the testing nd reporting of β-lctm ntiiotics for Grm-negtive orgnisms. Orgnism AMP AMC ATM CAZ CXM,CL,CPD CRO,CTX,KZ Antiiotic,c CPO CTT FEP FOX IPM,MEM ETP PRL TIM TZP Enteroctericee with n inducile AmpC d R R R R R T R R T R R R Aeromons with A2 crpenemse (most A. sori) e R R T T T T T T R e R R R Aeromons with A1 cephlosporinse & A2 crpenemse Providenci sturtii /rettgeri Morgnell. morgnii R R T R R T R R R R R R R R T R R T R R T R R T Proteus vulgris / penneri f R U R T R f R T T T R U U K. oxytoc K1 hyperproducer g R U R T g R g R T T T R U U Enteroctericee with n AmpC R R R R R T R R T R R R Enteroctericee with ESBL R U R R R R T R T R U U Pseudomons species / Burkholderi species h / Chryseocterium species R R h T T R T R R T T T T P. eruginos with ESBL R R R R R R R R T R U U Orgnism with metllo-β-lctmse R R T R R R R R R R R R Stenotrophomons mltophili i R R R i R i R R R R R R i R i R i c d e f g h i Where n orgnism fits into more thn one ctegory resistnce (R) to n ntiiotic in ny one ctegory tkes precedence over the remining ctegories. R = The orgnism is resistnt to the ntiiotic ecuse it possesses mechnism of resistnce tht my not e demonstrted y disc testing. T = Cn e tested. U = Test isoltes from urine only. Isoltes from other sites re considered RESISTANT. AMP mpicillin CL cephlexin CTX cefotxime KZ cefzolin AMC ugmentin CPD cefpodoxime ETP ertpenem MEM meropenem ATM ztreonm CPO cefpirome FEP cefepime PRL pipercillin CAZ ceftzidime CRO ceftrixone FOX cefoxitin TIM Timentin CXM cefuroxime CTT cefotetn IPM imipenem TZP Tzocin Enterocter erogenes, Enterocter cloce, Serrti mrcescens, Citrocter freundii, Hfni lvei. A. cvie does not possess crpenemse (A2) nd cn e tested ginst imipenem, meropenem nd ertpenem. Isoltes with high β-lctmse ctivity my give no zone round CTX 5 µg ut show "key-hole" effect tht my e mistken s n indiction of the presence of n ESBL. However, unlike ESBL producers, they my e susceptile to ceftzidime. K. oxytoc hyperproducer of the K1 enzyme my hve reduced zone of inhiition round cefotxime/ceftrixone disc. However, the orgnism is usully susceptile to ceftzidime nd cn e tested. B. pseudomllei is usully susceptile to Augmentin nd cn e tested ginst this ntiiotic. See Section 5.11 of the CDS mnul for notes on testing S. mltophili ginst these nd other ntiiotics. Online Edition Septemer
Survey of bloodstream infection isolates: SENTRY Antimicrobial Surveillance Program in Buenos Aires, Argentina ( )
Jordá Vrgs L, et l Survey of bloodstrem infection isoltes... rtículo originl/rtigo originl Survey of bloodstrem infection isoltes: SENTRY Antimicrobil Surveillnce Progrm in Buenos Aires, Argentin (1997-2002)
More informationPostantibiotic Sub-MIC Effects of Vancomycin, Roxithromycin, Sparfloxacin, and Amikacin
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Sept. 1992, p. 1852-1858 0066-4804/92/091852-07$02.00/0 Copyright X) 1992, Americn Society for Microiology Vol. 36, No. 9 Postntiiotic Su-MIC Effects of Vncomycin,
More informationESCMID Online Lecture Library. by author
Expert rules in susceptibility testing EUCAST-ESGARS-EPASG Educational Workshop Linz, 16 19 September, 2014 Dr. Rafael Cantón Hospital Universitario Ramón y Cajal SERVICIO DE MICROBIOLOGÍA Y PARASITOLOGÍA
More informationRoutine internal quality control as recommended by EUCAST Version 3.1, valid from
Routine internal quality control as recommended by EUCAST Version.1, valid from 01-01-01 Escherichia coli Pseudomonas aeruginosa Staphylococcus aureus Enterococcus faecalis Streptococcus pneumoniae Haemophilus
More informationEUCAST recommended strains for internal quality control
EUCAST recommended strains for internal quality control Escherichia coli Pseudomonas aeruginosa Staphylococcus aureus Enterococcus faecalis Streptococcus pneumoniae Haemophilus influenzae ATCC 59 ATCC
More informationHigh Frequency of Antimicrobial Resistance in Human Fecal Flora
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Dec. 1988, p. 181-186 66-484188112181-6$2./ Copyright 1988, Americn Society for Microbiology Vol. 32, No. 12 High Frequency of Antimicrobil Resistnce in Humn Fecl
More informationFilmArray Blood Culture Identification Panel Quick Guide
FilmArry Pnel FilmArry Blood Culture Identifiction Pnel Quick Guide Testing on the FilmArry should be performed within 8 hours of the positive reding from continuously monitoring blood culture instrument.
More informationWhat s new in EUCAST methods?
What s new in EUCAST methods? Derek Brown EUCAST Scientific Secretary Interactive question 1 MIC determination MH-F broth for broth microdilution testing of fastidious microorganisms Gradient MIC tests
More informationEuropean Committee on Antimicrobial Susceptibility Testing
European Committee on Antimicrobial Susceptibility Testing Routine and extended internal quality control as recommended by EUCAST Version 5.0, valid from 015-01-09 This document should be cited as "The
More informationCompliance of manufacturers of AST materials and devices with EUCAST guidelines
Compliance of manufacturers of AST materials and devices with EUCAST guidelines Data are based on questionnaires to manufacturers of materials and devices for antimicrobial susceptibility testing. The
More informationCompliance of manufacturers of AST materials and devices with EUCAST guidelines
Compliance of manufacturers of AST materials and devices with EUCAST guidelines Data are based on questionnaires to manufacturers of materials and devices for antimicrobial susceptibility testing. The
More informationHelp with moving disc diffusion methods from BSAC to EUCAST. Media BSAC EUCAST
Help with moving disc diffusion methods from BSAC to EUCAST This document sets out the main differences between the BSAC and EUCAST disc diffusion methods with specific emphasis on preparation prior to
More information2 New products for 2014
2 New products for 2014 Group B Streptococci Testing Thermo Scientific Brilliance GBS Agar Thermo Scientific TM Brilliance GBS Agar is a clear, selective chromogenic medium for the presumptive identification
More informationRomain Béraud, Louis Huneault, Dave Bernier, Francis Beaudry, Ann Letellier, Jérôme R.E. del Castillo. Abstract. Résumé
Article Comprison of the selection of ntimicroil resistnce in fecl Escherichi coli during enrofloxcin dministrtion with locl drug delivery system or with intrmusculr injections in swine model Romin Bérud,
More informationEUCAST-and CLSI potency NEO-SENSITABS
EUCASTand CLSI potency NEOSENSITABS Neo Sensitabs Page 1 / 6 Document: 6.2.0 Fastidious organisms EUCAST Interpretation zones and MIC breakpoints according to recommendations by the "Comité de l'antibiogramme
More informationSusceptibility of Anaerobic Bacteria to 23 Antimicrobial
ArTiMIctoIBAL AGoNT8 AND CHumOrHzRAPY, Oct. 1976, p. 736-752 Copyright 0 1976 Americn Society for Microbiology Vol. 10, No. 4 Printed in U.S.A. Susceptibility of Anerobic to 23 Antimicrobil Agents VERA
More informationEuropean Committee on Antimicrobial Susceptibility Testing
European Committee on Antimicrobial Susceptibility Testing Routine and extended internal quality control for MIC determination and disk diffusion as recommended by EUCAST Version 8.0, valid from 018-01-01
More informationSuggestions for appropriate agents to include in routine antimicrobial susceptibility testing
Suggestions for appropriate agents to include in routine antimicrobial susceptibility testing These suggestions are intended to indicate minimum sets of agents to test routinely in a diagnostic laboratory
More informationAntimicrobial susceptibility
Antimicrobial susceptibility PATTERNS Microbiology Department Canterbury ealth Laboratories and Clinical Pharmacology Department Canterbury District ealth Board March 2011 Contents Preface... Page 1 ANTIMICROBIAL
More informationThe ARESC study: an international survey on the antimicrobial resistance of pathogens involved in uncomplicated urinary tract infections
The ARESC study: n interntionl survey on the ntimicrobil resistnce of pthogens involved in uncomplicted urinry trct infections Gin Crlo Schito, Kurt G. Nber, Henry Botto, Jun Plou, Teresit Mzzei, Lur Gulco,
More informationESCMID Online Lecture Library. by author
Quality Assurance of antimicrobial susceptibility testing Derek Brown EUCAST Scientific Secretary ESCMID Postgraduate Education Course, Linz, 17 September 2014 Quality Assurance The total process by which
More information2012 ANTIBIOGRAM. Central Zone Former DTHR Sites. Department of Pathology and Laboratory Medicine
2012 ANTIBIOGRAM Central Zone Former DTHR Sites Department of Pathology and Laboratory Medicine Medically Relevant Pathogens Based on Gram Morphology Gram-negative Bacilli Lactose Fermenters Non-lactose
More information2015 Antibiotic Susceptibility Report
Citrobacter freundii Enterobacter aerogenes Enterobacter cloacae Escherichia coli Haemophilus influenzenza Klebsiella oxytoca Klebsiella pneumoniae Proteus mirabilis Pseudomonas aeruginosa Serratia marcescens
More information2016 Antibiotic Susceptibility Report
Fairview Northland Medical Center and Elk River, Milaca, Princeton and Zimmerman Clinics 2016 Antibiotic Susceptibility Report GRAM-NEGATIVE ORGANISMS 2016 Gram-Negative Non-Urine The number of isolates
More informationPrevalence and Antimicrobial Resistance of Enterococcus Species Isolated from Retail Meats
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Dec. 2003, p. 7153 7160 Vol. 69, No. 12 0099-2240/03/$08.00 0 DOI: 10.1128/AEM.69.12.7153 7160.2003 Prevlence nd Antimicrobil Resistnce of Enterococcus Species Isolted
More informationAMR Industry Alliance Antibiotic Discharge Targets
AMR Industry Alliance Antibiotic Discharge Targets List of Predicted No-Effect Concentrations (PNECs) The members of the AMR Industry Alliance have developed a unified approach to establishing discharge
More informationMain objectives of the EURL EQAS s
EQAS Enterococci, Staphylococci and E. coli EURL workshop, April, 11 Lourdes García Migura Main objectives of the EURL EQAS s To improve the comparability of antimicrobial susceptibility testing (AST)
More informationProduct Catalogue 2017 Clinical and Industrial Microbiology
Antibiotic discs in cartridge Description µg CLSI 1,2 EUCAST 3,4 BSAC Packaging * Ref. Amikacin AK x0 Discs 9004 Amoxicillin AML 2 x0 Discs 911 Amoxicillin AML x0 Discs 9133 Amoxicillin AML 2 x0 Discs
More informationPrevalence of Darkling Beetles (Alphitobius diaperinus) and Bacterial Load in Broiler Litters
Interntionl Journl of Poultry Science 6 (6): 440-444, 007 ISSN 168-8356 Asin Network for Scientific Informtion, 007 Prevlence of Drkling Beetles (Alphitoius diperinus) nd Bcteril Lod in Broiler Litters
More informationAntimicrobial Susceptibility Testing: Advanced Course
Antimicrobial Susceptibility Testing: Advanced Course Cascade Reporting Cascade Reporting I. Selecting Antimicrobial Agents for Testing and Reporting Selection of the most appropriate antimicrobials to
More informationProduct Catalogue 2016 Clinical and Industrial Microbiology
Antibiotic discs in cartridge Description μg CLSI 1,2 EUCAST 3,4 BSAC Packaging * Ref. Amikacin AK x0 Discs 9004 Amoxicillin AML 2 x0 Discs 911 Amoxicillin AML x0 Discs 9133 Amoxicillin AML 2 x0 Discs
More informationHelen Heffernan and Rosemary Woodhouse Antibiotic Reference Laboratory
METHODS USED IN NEW ZEALAND DIAGNOSTIC LABORATORIES TO IDENTIFY AND REPORT EXTENDED-SPECTRUM β-lactamase- PRODUCING ENTEROBACTERIACEAE by Helen Heffernan and Rosemary Woodhouse Antibiotic Reference Laboratory
More informationAgreed by the Antimicrobial Advice ad hoc Expert Group (AMEG) 2 May Adopted by the CVMP for release for consultation 19 May 2016
27 July 2016 EMA/CVMP/CHMP/231573/2016 Committee for Medicinl Products for Veterinry use (CVMP) Committee for Medicinl Products for Humn Use (CHMP) Updted dvice on the use of colistin products in nimls
More informationBSAC standardized disc susceptibility testing method (version 8)
Journal of Antimicrobial Chemotherapy (2009) 64, 454 489 doi:10.1093/jac/dkp244 Advance Access publication 8 July 2009 BSAC standardized disc susceptibility testing method (version 8) J. M. Andrews* for
More informationBritish Society for Antimicrobial Chemotherapy
British Society for Antimicrobial Chemotherapy BSAC to actively support the EUCAST Disc Diffusion Method for Antimicrobial Susceptibility Testing in preference to the current BSAC Disc Diffusion Method
More informationCHARACTERISTICS ASSOCIATED WITH OUT CROSSING IN A SHORT DURATION IMPROVED RICE (Oryza sativa L) VARIETY AT307
CHARACTERISTICS ASSOCIATED WITH OUT CROSSING IN A SHORT DURATION IMPROVED RICE (Oryz stiv L) VARIETY AT307 Kumr APA 1, Dhnyke Nilnthi 1 *, Phthinyke BD 2 nd Sennyke SGJN 1 1 Deprtment of Agriculturl Biology,
More informationSMART WORKFLOW SOLUTIONS Introducing DxM MicroScan WalkAway System* ...
SMART WORKFLOW SOLUTIONS Introducing DxM MicroScan WalkAway System* The next-generation MicroScan WalkAway System combines proven technology and reliability with enhanced ease-of-use features to streamline
More informationReal Life Problems involving Area
Rel Life Prolems involving Are Prolems occur often in everydy life. Exmple :- A edroom wll is to e wllppered. (300 cm) The wll hs een mesured nd is 6 metres y etres, s shown. The rolls of wllpper to e
More information2009 ANTIBIOGRAM. University of Alberta Hospital and the Stollery Childrens Hospital
2009 ANTIBIOGRAM University of Alberta Hospital and the Stollery Childrens Hospital Division of Medical Microbiology Department of Laboratory Medicine and Pathology 2 Table of Contents Page Introduction.....
More informationDistribution and dissemination of antimicrobial-resistant Salmonella in broiler farms with or without enrofloxacin use
Shng et l. BMC Veterinry Reserch (2018) 14:257 https://doi.org/10.1186/s12917-018-1590-1 RESEARCH ARTICLE Open Access Distribution nd dissemintion of ntimicrobil-resistnt Slmonell in broiler frms with
More informationComparative Study on Production Efficiency of Two Strains of Brown and White Egg Laying Hens in Kuwait
Interntionl Journl of Poultry Science 12 (7): 383-389, 2013 ISSN 1682-8356 Asin Network for Scientific Informtion, 2013 Comprtive Study on Production Efficiency of Two Strins of Brown nd White Egg Lying
More informationSusceptibility Patterns of Bacterial Pathogens Associated With Otitis Media At Murtala Muhammad Specialist Hospital, Kano, Nigeria
Susceptibility Ptterns of Bcteril Pthogens Associted With Otitis Medi At Murtl Muhmmd Specilist Hospitl, Kno, igeri A. H. Kwo* 1, B. M. Diyb 1, M. Yush u 1 * 1 Microbiology Unit, Deprtment of Biologicl
More informationa. 379 laboratories provided quantitative results, e.g (DD method) to 35.4% (MIC method) of all participants; see Table 2.
AND QUANTITATIVE PRECISION (SAMPLE UR-01, 2017) Background and Plan of Analysis Sample UR-01 (2017) was sent to API participants as a simulated urine culture for recognition of a significant pathogen colony
More informationStreptococcus pneumoniae. Oxacillin 1 µg as screen for beta-lactam resistance
Streptococcus pneumoniae Oxacillin µg as screen for beta-lactam resistance Version 6. June Streptococcus pneumoniae and zone diameter correlates The following histograms present inhibition zone diameter
More informationVersion 1.01 (01/10/2016)
CHN58: ANTIMICROBIAL SUSCEPTIBILITY TESTING (CLSI) 1.0 PURPOSE / INTRODUCTION: 1.1 Introduction Antimicrobial susceptibility tests are performed in order to determine whether a pathogen is likely to be
More informationC.D.S. USERS GROUP. NEWSLETTER No. 3. Report of the CDS Users Group Workshop held at the. ASM Gold Coast Meeting 1991
THE PRINCE OF WALES HOSPITAL The Prince of Wales Hospital, Cnr. High & Avoca Streets, Randwick. N.S.W. 2031. SMB/BG 20 th August, 1991. Dear Colleague, C.D.S. USERS GROUP NEWSLETTER No. 3 Report of the
More informationBritish Society for Antimicrobial Chemotherapy
British Society for Antimicrobial Chemotherapy Standing Committee on Susceptibility Testing Version 13.0, 10-06-2014 Content Page Additional information Changes in version 13 2 Suggestions for appropriate
More informationChemotherapy of bacterial infections. Part II. Mechanisms of Resistance. evolution of antimicrobial resistance
Chemotherapy of bacterial infections. Part II. Mechanisms of Resistance evolution of antimicrobial resistance Mechanism of bacterial genetic variability Point mutations may occur in a nucleotide base pair,
More information2010 ANTIBIOGRAM. University of Alberta Hospital and the Stollery Children s Hospital
2010 ANTIBIOGRAM University of Alberta Hospital and the Stollery Children s Hospital Medical Microbiology Department of Laboratory Medicine and Pathology Table of Contents Page Introduction..... 2 Antibiogram
More informationEffect of Rumensin on Health and Reproduction of Lactating Dairy Cows
Scientific Updte From Elnco Animl Helth Effect of Rumensin on Helth nd Reproduction of Lctting Diry Cows NADA 095-735 Dvid G. McClry, DVM, MS; Howrd B. Green, MS; Gerld D. Mechor, DVM; nd John I. D. Wilkinson,
More informationAntibiotic. Antibiotic Classes, Spectrum of Activity & Antibiotic Reporting
Antibiotic Antibiotic Classes, Spectrum of Activity & Antibiotic Reporting Any substance of natural, synthetic or semisynthetic origin which at low concentrations kills or inhibits the growth of bacteria
More informationجداول میکروارگانیسم های بیماریزای اولویت دار و آنتی بیوتیک های تعیین شده برای آزمایش تعیین حساسیت ضد میکروبی در برنامه مهار مقاومت میکروبی
جداول میکروارگانیسم های بیماریزای اولویت دار و آنتی بیوتیک های تعیین شده برای آزمایش تعیین حساسیت ضد میکروبی در برنامه مهار مقاومت میکروبی ویرایش دوم بر اساس ed., 2017 CLSI M100 27 th تابستان ۶۹۳۱ تهیه
More informationISSN: Isolation of High Antibiotic Resistant Fecal Bacteria Indicators, Salmonella and Vibrio Species from Raw
ISSN: 2276-7762 Isoltion of High Antibiotic Resistnt Fecl Bcteri Indictors, Slmonell nd Vibrio Species from Rw Abttoirs Sewge in Peri- Urbn Loctions of Nirobi, Keny By Nymboy Rosemry Atieno Okemo Pul Owuor
More informationLuteolysis and pregnancy outcomes after change in dose delivery of prostaglandin F2α in a 5-day timed artificial insemination program in dairy cows
Knss Agriculturl Experiment ttion Reserch Reports Volume Issue 2 Diry Reserch (94-24) Article 9 24 Luteolysis nd pregnncy outcomes fter chnge in dose delivery of prostglndin F2α in -dy timed rtificil insemintion
More informationThe Anatomy of Sea Turtles
Close this window to return to the previous pge or go to www.ivis.org The Antomy of Se Turtles Jenette Wyneken, Ph.D. Illustrted y Dwn Witherington Close this window to return to the previous pge or go
More informationSynergistic effect of rhein in combination with ampicillin or oxacillin against methicillin-resistant Staphylococcus aureus
608 Synergistic effect of rhein in combintion with mpicillin or oxcillin ginst methicillin-resistnt Stphylococcus ureus DAE-KI JOUNG 1, HEE JOUNG 1, DA-WUN YANG 1, DONG-YEUL KWON 2, JANG-GI CHOI 2, SEO
More informationUnderstanding the Hospital Antibiogram
Understanding the Hospital Antibiogram Sharon Erdman, PharmD Clinical Professor Purdue University College of Pharmacy Infectious Diseases Clinical Pharmacist Eskenazi Health 5 Understanding the Hospital
More informationAntibiotics: mode of action and mechanisms of resistance. Slides made by Special consultant Henrik Hasman Statens Serum Institut
Antibiotics: mode of action and mechanisms of resistance. Slides made by Special consultant Henrik Hasman Statens Serum Institut This presentation Definitions needed to discuss antimicrobial resistance
More informationEvaluation of the Hologic Gen-Probe PANTHER, APTIMA Combo 2 Assay in a Tertiary Care Teaching Hospital
AJCP / Originl Article Evlution of the Hologic Gen-Probe PANTHER, APTIMA Combo 2 Assy in Tertiry Cre Teching Hospitl Annie Cheng, MT, nd Jmes E. Kirby, MD From the Deprtment of Pthology, Beth Isrel Deconess
More informationPrinciples and Practice of Antimicrobial Susceptibility Testing. Microbiology Technical Workshop 25 th September 2013
Principles and Practice of Antimicrobial Susceptibility Testing Microbiology Technical Workshop 25 th September 2013 Scope History Why Perform Antimicrobial Susceptibility Testing? How to Perform an Antimicrobial
More informationInsecticide Resistance of the Green Rice Leafhopper, Nephotettix cincticeps, to the Systemic Insecticides Used for Seedling-Box Application
ScienceAsi 31 (25): 151-158 Insecticide Resistnce of the Green Rice Lefhopper, Nephotettix cincticeps, to the Systemic Insecticides Used for Seedling-Box Appliction Jirpong Jirin,* Nouki Kojim nd Toru
More informationDefining Extended Spectrum b-lactamases: Implications of Minimum Inhibitory Concentration- Based Screening Versus Clavulanate Confirmation Testing
Infect Dis Ther (2015) 4:513 518 DOI 10.1007/s40121-015-0094-6 BRIEF REPORT Defining Extended Spectrum b-lactamases: Implications of Minimum Inhibitory Concentration- Based Screening Versus Clavulanate
More informationESBL- and carbapenemase-producing microorganisms; state of the art. Laurent POIREL
ESBL- and carbapenemase-producing microorganisms; state of the art Laurent POIREL Medical and Molecular Microbiology Unit Dept of Medicine University of Fribourg Switzerland INSERM U914 «Emerging Resistance
More informationESBL Producers An Increasing Problem: An Overview Of An Underrated Threat
ESBL Producers An Increasing Problem: An Overview Of An Underrated Threat Hicham Ezzat Professor of Microbiology and Immunology Cairo University Introduction 1 Since the 1980s there have been dramatic
More informationCONTAGIOUS COMMENTS Department of Epidemiology
VOLUME XXXII NUMBER 6 September 2017 CONTAGIOUS COMMENTS Department of Epidemiology Bugs and Drugs Elaine Dowell SM MLS (ASCP), Stacey Hamilton MT SM (ASCP), Samuel Dominguez MD PhD, Sarah Parker MD, and
More informationShell Thickness of Turkey Eggs Affects Cardiac Physiology and Embryo Survival 1
Interntionl Journl of Poultry Science 5 (8): 796-80, 2006 ISSN 682-856 Asin Network for Scientific Informtion, 2006 Shell Thickness of Turkey Eggs Affects Crdic Physiology nd Emryo Survivl 2 2 4 2 V.L.
More informationInterpreting Microbiology reports for better Clinical Decisions Interpreting Antibiogrammes
Interpreting Microbiology reports for better Clinical Decisions Interpreting Antibiogrammes Prof C. Wattal Hon. Sr. Consultant & Chairman Dept. of Clinical Microbiology Sir Ganga Ram Hospital New Delhi
More informationA Model for Promoting Poultry Industry Development in Togo: Part 1. Management Practices and Incubation Conditions
Interntionl Journl of Poultry Science 13 (3): 176-184, 2014 ISSN 1682-8356 Asin Network for Scientific Informtion, 2014 A Model for Promoting Poultry Industry Development in Togo: Prt 1. Mngement Prctices
More informationThere are two international organisations that set up guidelines and interpretive breakpoints for bacteriology and susceptibility
ANTIMICROBIAL SUSCEPTIBILITY TESTING ON MILK SAMPLES Method and guidelines There are two international organisations that set up guidelines and interpretive breakpoints for bacteriology and susceptibility
More informationQuality assurance of antimicrobial susceptibility testing
Quality assurance of antimicrobial susceptibility testing Derek Brown Routine quality control Repeated testing of controls in parallel with tests to ensure that the test system is performing reproducibly
More information2017 Antibiogram. Central Zone. Alberta Health Services. including. Red Deer Regional Hospital. St. Mary s Hospital, Camrose
2017 Antibiogram Central Zone Alberta Health Services including Red Deer Regional Hospital St. Mary s Hospital, Camrose Introduction This antibiogram is a cumulative report of the antimicrobial susceptibility
More informationAntimicrobial Susceptibility Testing: The Basics
Antimicrobial Susceptibility Testing: The Basics Susan E. Sharp, Ph.D., DABMM, FAAM Director, Airport Way Regional Laboratory Director, Regional Microbiology and Molecular Infectious Diseases Laboratories
More information2015 Antibiogram. Red Deer Regional Hospital. Central Zone. Alberta Health Services
2015 Antibiogram Red Deer Regional Hospital Central Zone Alberta Health Services Introduction. This antibiogram is a cumulative report of the antimicrobial susceptibility rates of common microbial pathogens
More informationMASTDISCS AST. Leading the field with a complete solution for AST and Identification disc testing. Comprehensive range. Premium quality products
IVD solutions through partnership MASTDISCS AST Leading the field with a complete solution for AST and Identification disc testing Comprehensive range Premium quality products Compatible with EUCAST and
More information1 INTRODUCTION OBJECTIVES OUTLINE OF THE SALM/CAMP EQAS
PROTOCOL For antimicrobial susceptibility testing of Salmonella, Campylobacter and optional genotypic characterisation of AmpC-, ESBL- and carbapenemase-producing test strains 1 INTRODUCTION... 1 2 OBJECTIVES...
More informationVersion 10.1 April 2011
BSAC Methods for Antimicrobial Susceptibility Testing Version 10.1 April 2011 All enquiries to: Jenny Andrews at: + 44 (0) 121 507 5693 Email: jenny.andrews1@nhs.net Contents Page Working Party members
More informationEnlargement 2. Scale and Enlargement
Scle nd Enlrgement Enlrgement 2 Here is picture of Andy. He is 7 5 cm tll in the picture. In rel life, Andy is ctully twenty times the size he ppers to e in the picture. We sy tht this picture hs scle
More informationVersion 8 January 2009
BSAC Methods for Antimicrobial Susceptibility Testing All enquiries to: Jenny Andrews at: + 44 (0) 121 507 5693 Email: jenny.andrews@swbh.nhs.uk 2 Contents Page Working Party members 5 Abstract 6 Preface
More informationConcise Antibiogram Toolkit Background
Background This toolkit is designed to guide nursing homes in creating their own antibiograms, an important tool for guiding empiric antimicrobial therapy. Information about antibiograms and instructions
More informationEfficacy of Clarithromycin for Treatment of Experimental
ANTIMICROBLAL AGENTS AND CHEMOTHERAPY, June 1993, p. 1329-1333 0066-4804/93/061329-05$02.00/0 Copyright X) 1993, Americn Society for Microbiology Vol. 37, No. 6 Efficcy of for Tretment of Experimentl Lyme
More informationSurveillance for antimicrobial resistance in enteric bacteria in Australian pigs and chickens
Surveillance for antimicrobial resistance in enteric bacteria in Australian pigs and chickens Dr Pat Mitchell R & I Manager Production Stewardship APL CDC Conference, Melbourne June 2017 Dr Kylie Hewson
More informationPROTOCOL for serotyping and antimicrobial susceptibility testing of Salmonella test strains
PROTOCOL for serotyping and antimicrobial susceptibility testing of Salmonella test strains 1 INTRODUCTION... 1 2 OBJECTIVES... 2 3 OUTLINE OF THE EQAS 2017... 2 3.1 Shipping, receipt and storage of strains...
More informationVersion 9.1 March 2010
BSAC Methods for Antimicrobial Susceptibility Testing Version 9.1 March 2010 All enquiries to: Jenny Andrews at: + 44 (0) 121 507 5693 Email: jenny.andrews@swbh.nhs.uk Contents Page Working Party members
More informationNon-Susceptibility of Bacterial Pathogens Causing Hospital-Onset Pneumonia UK and Ireland,
Non-Susceptibility of Bacterial Pathogens Causing Hospital-Onset Pneumonia UK and Ireland, 2008-2016 Alicia Russell Federation of Infection Societies conference 14 th November 2018 alisia_russell BSAC
More informationTaiwan Surveillance of Antimicrobial Resistance (TSAR)
Taiwan Surveillance of Antimicrobial Resistance (TSAR) 2009 MIRL Symposium July 17, 2009 Tsai-Ling Yang Lauderdale ( ) Microbial Infections Reference Laboratory (MIRL) Division of Infectious Diseases,
More informationPolicy # MI_AST Department of Microbiology. Page Quality Manual
Department of Microbiology Version: 2.0 CURRENT 1 of 160 Prepared by QA Committee Issued by: Laboratory Manager Revision Date: 2/26/2018 Approved by Laboratory Director: Annual Review Date: 5/1/2018 Microbiologist-in-Chief
More informationAntimicrobial susceptibility testing challenges. Linda Joyce St Vincent s Hospital Melbourne
Antimicrobial susceptibility testing challenges Linda Joyce St Vincent s Hospital Melbourne Bacteria/antimicrobials without breakpoints (B.A.W.B.S.) Enterobacteriacae Pseudomonas aeruginosa, Acinetobacter
More informationEfficacy of Colistin in combination with Carbapenem and Tigecycline in patients with pneumonia caused by multidrug-resistant Acinetobacter baumannii
Efficacy of Colistin in combination with Carbapenem and Tigecycline in patients with pneumonia caused by multidrug-resistant Acinetobacter baumannii Enty Tjoa 1, Frans Pangalila 2, Lucky H Moehario 1,
More informationIntrinsic, implied and default resistance
Appendix A Intrinsic, implied and default resistance Magiorakos et al. [1] and CLSI [2] are our primary sources of information on intrinsic resistance. Sanford et al. [3] and Gilbert et al. [4] have been
More informationEUCAST expert rules in antimicrobial susceptibility testing
REVIEW BACTERIOLOGY EUCAST expert rules in antimicrobial susceptibility testing R. Leclercq 1,2, R. Cantón 2,3,4, D. F. J. Brown 4, C. G. Giske 2,4,5, P. Heisig 2,6, A. P. MacGowan 4,7, J. W. Mouton 4,8,
More informationOriginal Article. Introduction
Irnin Journl of Phrmceuticl Reserch (2018), 17 (4): 1182-1190 Received: Mrch 2016 Accepted: Decemer 2016 Originl Article Determintion of Enrofloxcin nd Ciprofloxcin Residues in Five Different Kinds of
More informationPenicillins - EUCAST clinical MIC breakpoints (version 1.3)
EUCAST clinical MIC breakpoints - penicillins Penicillins - EUCAST clinical MIC breakpoints 2009-04-19 (version 1.3) Penicillins Click on antibiotic name to see wild type MIC distributions. Enterobacteriaceae
More informationAntimicrobials. Antimicrobials
Antimicrobials For more than 50 years, antibiotics have come to the rescue by routinely producing rapid and long-lasting miracle cures. However, from the beginning antibiotics have selected for resistance
More informationTABLE OF CONTENTS. Urine - Gram Positive Susceptibility Reporting 1 Staphylococcus species, MRSA...11
Policy #MI\ANTI\v23 Page 1 of 3 Section: Antimicrobial Susceptibility Testing Subject Title: Table of Contents Manual Issued by: LABORATORY MANAGER Original Date: January 10, 2000 Approved by: Laboratory
More informationNational Clinical Guideline Centre Pneumonia Diagnosis and management of community- and hospital-acquired pneumonia in adults
National Clinical Guideline Centre Antibiotic classifications Pneumonia Diagnosis and management of community- and hospital-acquired pneumonia in adults Clinical guideline 191 Appendix N 3 December 2014
More informationAntibacterial therapy 1. د. حامد الزعبي Dr Hamed Al-Zoubi
Antibacterial therapy 1 د. حامد الزعبي Dr Hamed Al-Zoubi ILOs Principles and terms Different categories of antibiotics Spectrum of activity and mechanism of action Resistancs Antibacterial therapy What
More informationEinheit für pädiatrische Infektiologie Antibiotics - what, why, when and how?
Einheit für pädiatrische Infektiologie Antibiotics - what, why, when and how? Andrea Duppenthaler andrea.duppenthaler@insel.ch Limping patient local pain swelling tenderness warmth fever acute Osteomyelitis
More informationEffect of Dwarfism on Reproductive and Meat Yield Parameters of Crossbred Chicken
Interntionl Journl of Poultry Science 4 (6): 372-377, 2005 ISSN 1682-8356 Asin Network for Scientific Informtion, 2005 Effect of Dwrfism on Reproductive nd Met Yield Prmeters of Crossred Chicken 1 2 3
More informationBrief reports. Heat stability of the antimicrobial activity of sixty-two antibacterial agents
Journal of Antimicrobial Chemotherapy (5) 35, -5 Brief reports Heat stability of the antimicrobial activity of sixty-two antibacterial agents Walter H. Traub and Birgit Leonhard Institut fur Medizinische
More informationEARS-Net Belgium Data call for 2016: Instructions for participating laboratories, including data definition. (version 4, 20/3/2017)
EARS-Net Belgium Data call for 2016: Instructions for participating laboratories, including data definition. (version 4, 20/3/2017) Questions on this document can be directed towards: Karl Mertens Scientific
More information