OIE RL for Rabies in China: Activities and Challenges

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1 OIE RL for Rabies in China: Activities and Challenges Diagnostic Laboratory on Rabies and Wildlife Associated Zoonoses (DLR), Chinese Ministry of Agriculture; Changchun Vet Res Institute (CVRI), Chinese Academy of Agri Sci; OIE Reference Laboratory for Rabies;

2 1 Changchun Vet Res Institute (CVRI) Established in 1949 and located in Changchun, the capital city of Jilin Province. Focuses on preventive vet med and sci

3 Diagnostic Laboratory for Rabies and Wildlife Associated Zoonoses BSL2 537 M 2 in total BSL M 2 in total Established in 2005 OIE RL for Rabies in 2012

4 1 Diagnostic Laboratory for Rabies and Wildlife Associated Zoonoses PCR Cyclers Automatic RNA extraction Fluorescence microscope Ultra-centrifuge Cell culture

5 Technical capacity FAT RTCIT MIT 1 Ag detection 2 NA detection RT-PCR Real time PCR Phylogeny 3 Ab detection FAVN

6 Technical capacity

7 Main Activities Diagnosis of animal rabies cases Rapid response to the outbreak Epidemiology and surveillance Evaluation of rabies immunity for pet travel scheme Technical training and advice Scientific research

8 Main Activities Diagnosis, epidemiology and surveillance of rabies Animal rabies cases RABV genotypes in China Dog Asian 1 Asian 2 Arctic-related 亚洲 1 群 Asian 1 亚洲 2 群 Asian 2 北极相关群 Arctic-related 世界群 Cosmopolitan 世界群草原型 Steppe-type Cosmopolitan

9 Main Activities Rapid response to the outbreak Camel rabies Sheep rabies

10 Main Activities Evaluation of rabies immunity >1000 dogs and cats tested in Pet travel scheme + _ FAVN

11 Main Activities Technical training SEA countires 2014 Indonesia in 2016 National training 2013 Nepal in 2012

12 R & D: Main Activities 1. Direct rabies immunohistochemistry test (drit) Biotinylated mab FAT drit + drit - 2. Oligo DNA microarray Control RABV LBV MOKV DUVV EBLV-1 EBLV-2 ABLV

13 Main Activities OIE ad hoc meeting on rabies (2011 and 2012) OIE/FAO mission to Vietnam on rabies (2013) OIE consultance on Oral vaccination against dog rabies (2016) Vietnam rabies mission-2013 Ad hoc meeting-2011 Ad hoc meeting-2012

14 Publications Yu Jiang, et al.,seroprevalence of Rabies Virus Antibodies in Bats from Southern China,Vector-borne and Zoonotic Dis. 2010,10(2): Wenjie Gong, et al., Temporal and Spatial Dynamics of Rabies Viruses in China and Southeast Asia, Virus Res. 2010,150(1-2): Yan Zhu et al.,an outbreak of sheep rabies in Shanxi province, China. Epidemiol.Infect., 2011, doi: /s Jin Xi et al.,differentiation of the seven major lyssavirus species by oligonucleotide microarray, J Clin Microbiol, 2012,50(3): Yin, Wenwu et al., Challenges and needs for China to eliminate rabies. Infect Dis Poverty.2013, 2(1):23. Ye Feng eat al.,disease outbreaks caused by steppe-type rabies viruses in China, Epidemiol.Infect, 2014, 143(6): Hualei Wang et al., Using rabies virus vaccine strain SRV9 as viral vector to express exogenous gene, Virus Genes, 2015, 50(2): Ye Feng, et al., Livestock rabies outbreaks in Shanxi province,china, Arch Virol, 2016, 161:

15 Key Challenges In sample importation to provide diagnostic service to OIE Member Countries In techinical training to Member Countries In laboratory network with other Reference Centers

16 Challenge 1 In sample importation The official permit to import biosamples is given on case-by-case basis according to GAQSIQ s regulations All importing samples must pay tariffs (even they are free) according to GAC s regulations. Therefore, precedures are time-consuming and costly for us to receive samples outside China, eventually prevents us from providing diagnostic service to OIE Member Countries GAC GAQSIQ General Administration of Customs of China General Administration of Quality Supervision, Inspection and Quarantine of China

17 Challenge 2 In techinical training Techinical training is very important to help member countries build up it s laboratory capacity. Demands and requests increasing, but no program and funding in place. Nepal in 2012

18 Challenge 3 In network The laboratory network is weak to facilitate the sharing and exchanging of knowledge and expertise among OIE RLs.

19 Suggestions For challenge 1,we continue to solicit MoA to coordinate with GAQSIQ and GAC. For challenge 2, I would suggest to establish OIE fund to specially support technical training in OIE RLs for personnels of the Member Countries For challenge 3, I would suggest to establish annual meeting mechanism for Regional OIE RLs, which can be organized in turn in the country of each RL under the support of OIE and/or host country.

20 Thank you for your listening and comments!