Disk Susceptibility Studies with Cefazolin and Cephalothin
|
|
- Martha Fitzgerald
- 6 years ago
- Views:
Transcription
1 ANTIMICROBiAL AGENTS AND CHEMOTHEMRAPY, Jan. 1974, p Copyright i 1974 American Society for Microbiology Vol. 5, No. 1 Printed in U.SA. Disk Susceptibility Studies with Cefazolin and Cephalothin PAUL ACTOR, JOSEPH GUARINI, JOSEPH URI, JUDITH DICKSON, JOHN F. PAULS, AND JERRY A. WEISBACH Smith Kline & French Laboratories, Philadelphia, Pennsylvania Received for publication 14 September 1973 Cefazolin and cephalothin disk susceptibility and minimal inhibitory concentration determinations were conducted on 591 clinical isolates. Cefazolin demonstrated superior activity, as shown by lower minimal inhibitory concentrations, and a greater percentage of isolates inhibited in the disk susceptibility test. The cephalothin antibiotic class disk by the standard Bauer-Kirby method failed to detect susceptibility to cefazolin in a significant percentage of Escherchia coli, Enterobacter species, and Enterococcus isolates. A separate cefazolin disk with a susceptibility cut-off point of 18 mm is recommended. An alternative to a separate cefazolin disk would be a reinterpretation of the cephalothin susceptibility disk zone diameters so that it would more adequately predict cefazolin activity. Standard regulatory methods for tfie testing of antibiotic disk susceptibility have been published in the Federal Register (3). Representing the cephalosporin antibiotics is a disk containing 30,g of cephalothin. This disk is to be used in the determination of cephalothin, cephaloridine, cephaloglycine, and cephalexin susceptibilities. The use of such a representative, or class, disk assumes that it will cover adequately the susceptibilities within the antibiotic class. Because our laboratory is in the process of developing a new cephalosporin, cefazolin, we decided to undertake studies to compare the 30-,gg cephalothin disk with a 30-/ig cefazolin disk. The primary purpose of these studies was to determine the adequacy of the cephalosporin class disk in predicting cefazolin response as well as to detect potential differences in the two antibiotic disks. A battery of susceptibility tests comparing the two antibiotic disks was performed against a variety of gram-positive and gram-negative clinical bacterial isolates. In addition, the minimal inhibitory concentrations of cefazolin and cephalothin for each isolate were determined in order to correlate these values with the data obtained in the disk susceptibility studies. MATERIALS AND METHODS Bacterial isolates. Isolates used in these studies were obtained from hospitalized patients and sent to our laboratory from a variety of geographical locations in the United States. These samples included some isolates from patients later treated with cefazolin. Upon receipt, the isolates were classified and tested for response to 12 commercial antibiotics. A single passage of the organism was made, and samples were frozen at -70 C for additional trials. Antibiotic disks. Cephalothin 30-,ug disks were purchased from BBL. The 30-iAg cefazolin disks were prepared in the BBL laboratories under controlled conditions conforming to their commercial production of susceptibility disks. The disks were assayed for stability over a 1-year period in both BBL and our laboratories and found to have no significant loss in potency when stored at 4 C. The assays were performed by the FDA performance plate test method described in the Federal Register for cephalosporin disks (2). Disk susceptibility tests. The procedure employed was essentially that of Bauer and Kirby (1) and later was detailed in the Federal Register (3). Clinical isolates first were grown in Trypticase soy broth, adjusted to a standard optical density, and swabbed on the surface of a petri dish containing Mueller-Hinton agar (BBL). Duplicate disks were placed on the agar surface with sterile forceps. All plates were incubated at 37 C for 18 h. Zones of inhibition that developed were measured with a Fisher-Lilly zone rqader. A duplicate test was carried out on another day to account for day-to-day variation. In each experiment, two stock reference strains, one of Staphylococcus aureus (ATCC 25923) and one of Escherichia coli (ATCC 25922), were included to establish the validity of the experiment. By using the abovedescribed techniques, the acceptable limits for the 30 Ag cephalothin disk is a zone range of 25 to 37 mm for S. aureus and a 18 to 23 mm range for E. coli. Minimal inhibitory concentrations (agar dilution method). The agar dilution tests were carried out in Trypticase soy agar containing 0.5% glucose and buffered to ph 6.0. Stock solutions of cephalothin and cefazolin were prepared in sterile water, and 12 '33 twofold dilutions were added to petri dishes, result-
2 64 ACTOR ET AL. ANTIMICROB. AG. CHEMOTHER. ing in final antibiotic concentrations ranging from 0.1 to 200 yg per ml of agar. Approximately 104 gramnegative or 106 gram-positive organisms were inoculated in duplicate onto the agar with a Steers replicating apparatus (11). After incubation at 37 C for 18 h, the plates were examined, and the minimal quantity of compound capable of inhibiting growth (MIC) was determined. RESULTS MIC. The median MIC, obtained with cefazolin and cephalothin by the agar dilution method are shown in Table 1. A total of 591 isolates were included in this table. Values of 200 Ag/ml or greater were obtained with both antibiotics against indole-positive Proteus, Enterobacter hafniae, Serratia, and Pseudomonas aeruginosa. Cefazolin was found to give activity equal or superior to cephalothin against all of the other gram-negative organisms studied. The MIC values obtained for cephalothin against staphylococcus were approximately twofold better than cefazolin. A marked difference was observed with the Enterobacter species studied, where cefazolin was seen to be from 4 to 20 times more effective under the conditions used in these tests. Enterobacter aerogenes and Enterobacter liquefaciens seemed particularly susceptible to cefazolin activity. Disk diffusion studies. A total of four values were obtained with each of the 30-,gg cephalosporin disks against each of the isolates (duplicate disks on 2 test days). These four zone diameters were then averaged to give a single TABLE 1. Median minimal inhibitory concentrations of cefazolin and cephalothin against bacterial isolates Organism Escherichia coli Klebsiella pneumoniae Proteus mirabilis Proteus sp. (indole posi >200.0 tive) Enterobacter cloacae E. aerogenes E. liquefaciens E. hafniae 5 > > E. agglomerans Salmonella sp Shigella sp Serratia sp. 24 > > Pseudomonas aeruginosa 15 > > Enterococci Staphylococcus sp value for each isolate with each antibiotic. An estimate (by the least squares method) of the linear relationship between log MIC and zone sizes for the 30-.ug disks was then determined (Fig. 1 and 2). Only those isolates showing a Minimal inhibitory No. of concn (Mg/ml) isolates Cefazolin Cephalothin ENT. CLOACAE ENTEROCOCCI. PROTEUS (INDOL NEG.) SALMONELLAI ENT. AEROGENESI. KLEE. O - \~- 1.0 z E. C 0 N 4c w SA S~~~~~~~~TAPH U.- 1I I AVERAGE ZONE DIAMETER FOR CLINICAL ISOLATES WITH CEFAZOLIN DISC, mm FIG. 1. Relationship of zone diameters (30-ug disk) and minimal inhibitory concentration values obtained with cefazolin against clinical bacterial isolates. ENTEROCOCC E ENT. CLOACAE ENT. AEROGENES * - E. COLI SALMONELLA KLEB. * PROTEUS (INDOL NEG.) 2 0 OI 4 I U * <_ ~~~~~~~~~~STAPH.\ AVERAGE ZONE DIAMETER FOR CLINICAL ISOLATES WITH CEPHALOTHIN DISC. mm FIG. 2. Relationship of zone diameters (30-;&g disk) and minimal inhibitory concentration values obtained with cephalothin against clinical bacterial isolates.
3 VOL. 5, 1974 CEFAZOLIN AND CEPHALOTHIN DISK SUSCEPTIBILITY 65 positive zone of inhibition with a MIC within the test range were used in the comparison. Because the data were dominated by a few species of bacteria (i.e., E. coli and Enterobacter cloacae), we felt that a comparison based on species averages rather than on data for individual isolates would yield more representative results. Examination of the slopes obtained with the two antibiotics shows that at equivalent MIC values, the cefazolin 30-,ug disk tended to give a zone diameter somewhat less than that of cephalothin; however, there was no statistical difference in the slopes of the regression lines ( versus in logarithms) obtained with the two antibiotics. Figure 3 represents a plot of the log MIC for cefazolin plotted against the species average zone diameters for cephalothin. By using the equation given for the regression line, it can be calculated that the zone diameter of 14 mm for cephalothin will, on the average, correspond to a cefazolin MIC of 4.8,ug/ml. This MIC represents a concentration which is readily attainable in serum of patients treated with cefazolin, even with a 250-mg dose. Determination of susceptibility of isolates. The results of the Bauer-Kirby disk susceptibility tests are shown in Table 2, which shows the susceptibility patterns obtained using several parameters of classification for sensitive, intermediate, and resistant. A comparison of the data obtained with the 30-,gg cefazolin and cephalothin disks, by using 18 mm as the susceptibility cut-off point (SCOP), shows a number of significant differences with the various species of clinical isolates. In general, a much higher percentage of the isolates were found to be susceptible to the cefazolin disk : X 1.0- z 0 U. * ENT. CLOACAE SiENTEROCOCCI ENT. AEROGENES * KLEE E. COLI PROTEUS (INDOL NEG.) SALMONELLA I., lb 1 io is5 io STAPH. AVERAGE ZONE DIAMETER FOR CLINICAL ISOLATES WITH CEPHALOTHIN DISC, mm FIG. 3. Relationship of cephalothin zone diameters (30-jAg disk) and cefazolin minimal inhibitory concentration values obtained against clinical bacterial isolates. Notable examples include: E. coli, 95.7 versus 75.8%; E. aerogenes, 85.7 versus 19.1%; and enterococci, 35.4 versus 13.9%. If the parameters for susceptibility for the cephalothin disk are adjusted so that a zone size of > 14 mm is used for susceptibility classification, then the data obtained more closely approximate that obtained with the cafazolin disk when > 18 mm is used for susceptibility. DISCUSSION The MICs obtained with cefazolin and cephalothin (Table 1) are in general agreement with the values reported in the literature (6-10, 13). Differences observed can, in many cases, be attributed to differences in testing conditions such as inoculum size, media, strains of organisms, incubation time, and tube dilution versus agar dilution. The MIC values obtained with enterococcal and enterobacteral isolates are, in general, lower than those reported by other investigators. Reller et al. (7) have shown that the MIC for cefazolin for isolates of E. coli, Proteus mirabilis, Klebsiella, Salmonella, and Shigella were in close agreement when the broth or agar dilution techniques were employed. Some of their Enterobacter strains showed higher MIC values in broth dilution than did the enterococci. The lower MIC values obtained in our studies may, in part, be due to the use of the agar dilution technique. A marked difference in MIC response to cefazolin was observed with the five Enterobacter species studied. E. aerogenes and E. liquefaciens were found to be inhibited at low cefazolin concentration, whereas E. cloacae and Enterobacter agglomerans were inhibited at higher concentrations. The five strains of E. hafniae were completely resistant (MIC > 200 gg/ml) to both cafazolin and cephalothin. Most investigators do not speciate their Enterobacter isolates; therefore, it is difficult to compare our data with literature values. In addition, the Trypticase soy agar medium employed in our studies is buffered at ph 6.0, a condition that tends to result in lower MIC values with the cephalosporin antibiotics. The differences between the two antibiotics obtained with Enterobacter species are also observed in the disk susceptibility tests (Table 2). In any event, patients infected with strains of Enterobacter found to be susceptible to cefazolin were found to respond to cefazolin treatment (12, Gold et al., J. Infect. Dis., in press). The log of the MIC values for cefazolin and cephalothin were plotted against the average zone diameters for each species of clinical isolate studied (Fig. 1 and 2). Only those species
4 66 ACTOR ET AL. ANTIMICROB. AG. CHEMOTHER. TABLE 2. Percentage of bacterial isolates responding to cefazolin and cephalothin antibiotic disks No. of Cefazolin Diska Cephalothin Diska Cephalothin Disk" Organisms Isolates R R S R S Escherichia coli Enterobacter aerogenes E. cloacae E. liquefaciens E. hafniae E. agglomerans Proteus, indole negative Proteus, indole positive Klebsiella Enterococci Salmonella Shigella Staphylococcus a Resistant (R), < 14 mm; intermediate (I), 15 to I br, <10 mm; I 11 to 13 mm; S, >14 mm. where positive values were obtained for 10 or more isolates were used to generate the regression lines. The regression lines as calculated by the method of least squares are similar to values previously reported for these antibiotics (5, 13). The zone diameter values obtained with cefazolin are apparently smaller than those obtained with cephalothin at equivalent MIC determinations. This difference in zone size may be due to a difference in diffusion of the two antibiotics. A cefazolin 30-,ug susceptibility disk could employ the same parameters as cephalothin in the Bauer-Kirby disk susceptibility test: susceptible, > 18 mm; intermediate, mm; resistant, < 14 mm. A zone size of 18 mm for cephalothin would correspond to a MIC of 10.3 jig/ml on the regression line. Similarly, an 18-mm zone for cefazolin would indicate a MIC of 4.8 jg/ml, a level easily reached in the serum of men dosed intramuscularly with levels as low as 250 mg (6). By using the cefazolin disk with a susceptibility cut-off point of 18 mm, it was seen that 95.7% of the E. coli isolates were susceptible to cefazolin, whereas only 75.8% responded to cephalothin (Table 2). Large differences in susceptibility to the two antibiotics also were observed with E. aerogenes (85.7 versus 19.1%) and Enterococci (35.4 versus 13.9%). With E. coli and E. aerogenes isolates, cefazolin showed a four- to eightfold lower MIC; however, with Enterococci, there was no difference in the median MIC values for the two antibiotics. It should be noted that many of the enterococci fell into the intermediate category with both antibiotics. Cefazolin has been shown to be a useful antibiotic for treatment of enterococcal 17 mm; susceptible (S), >18 mm. infections in humans, and the disk data would tend to support this finding (Gold et al., J. Infect. Dis., in press). Isenberg et al. (4) have reported on a marked difference in response with enterococci with 30-gAg cephalothin and cephacetrile disks. Although cephacetrile consistently gave larger zone diameters in the Bauer-Kirby system, these differences rarely resulted in a change in the susceptibility category of an organism. The cephalothin response (zone diameter measurements) did not predict the cephacetrile response. The responses of individual isolates toward cephaloridine and cephaloglycine also were found to be different. As a result of these findings, we questioned the wisdom of using a single representative of a group of antibiotic agents to measure susceptibility toward all derivatives. A similar conclusion has been expressed by Wick and Preston (13), who worked with three heterocyclic thiomethyl cephalosporins, including cefazolin. They reasoned that the high serum levels attainable plus the extended activity spectrum argue against a class cephalosporin disk. An analysis of the available data, to obtain the regression line relating the logarithm of the cephazolin MIC and cephalothin disk diameter, can be used in reinterpreting the cephalothin zone diameter data so that it will predict cefazolin activity (Fig. 3). Specifically, it appears that the appropriate cephalothin zone diameters for predicting cefazolin susceptibility should be as follows: > 14 mm, susceptible; 11 to 13 mm, intermediate; < 10 mm, resistant. Thus, an alternative to a separate cefazolin susceptibility disk would be to reinterpret the SCOP for
5 VOL 5, 1974 CEFAZOLIN AND CEPHALOTHIN DISK SUSCEPTIBILITY 67 cephalothin zone diameters to predict cefazolin activity. It would appear that a SCOP of 14 mm would accomplish this end (Table 2). In almost all cases, a SCOP for cephalothin of 14 mm results in a pattern of response similar to that obtained with a cefazolin disk with a SCOP of 18 mm. The cephalothin disk still falls somewhat short of predicting E. aerogenes response; however, a larger than expected percentage of the enterococci are classified as susceptible to cefazolin. It would appear from these studies that the present class cephalosporin (cephalothin) disk fails to predict adequately cefazolin activity, particularly in the case of E. coli and E. aerogenes. In light of the superior serum levels observed in humans with cefazolin (three to four times that of cephalothin) after intramuscular injection and the improved microbiological activity, a separate cefazolin disk would be justified. An alternate approach to the problem would be to reinterpret the cephalothin class susceptibility disk zone sizes so that they adequately predict cefazolin activity. ACKNOWLEDGMENTS We thank Marie E. Knight and Bernard C. Sekula for their excellent technical assistance. Cefazolin was synthesized by the Fujisawa Pharmaceutical Compnay, Osaka, Japan. LITERATURE CITED 1. Bauer, A. W., W. M. Kirby, J. C. Sherris, and M. Turck Antibiotic susceptibility testing by a standardized single disc method. Amer. J. Clin. Pathol. 45: Department of Health, Education, and Welfare, Food and Drug Administration Fed. Regist. 32: Department of Health, Education and Welfare, Food and Drug Administration Fed. Regist. 37: Isenberg, H. D., B. G. Painter, J. Sampson-Scherer, and M. Siegel Clinical laboratory study of cephacetrile and cephalothin against bacteria recently isolated from clinical specimens. Amer. J. Clin. Pathol. 59: Matsen, J. M., M. J. H. Koepcke, and P. G. Quie Evaluation of the Bauer-Kirby-Sherris-Turck single disc diffusion method of antibiotic susceptibility testing. Antimicrob. Ag. Chemother. 1969, p Nishida, M., T. Matsubara, T. Murakawa, Y. Mine, Y. Yokota, S. Kuwahara, and S. Goto In vitro and in vivo evaluation of cefazolin, a new cephalosporin C derivative. Antimicrob. Ag. Chemother. 1969, p Reller, L. B., W. W. Karney, H. N. Beaty, K. K. Holmes, and M. Turck Evaluation of cefazolin, a new cephalosporin antibiotic. Antimicrob. Ag. Chemother. 3: Ries, K., M. E. Levison, and D. Kaye Clinical and in vitro evaluation of cefazolin, a new cephalosporin antibiotic. Antimicrob. Ag. Chemother. 3: Seiga, K., K. Yamaji, K. Miyoshi, and M. Minagawa Laboratory and clinical studies on cefazolin, a new derivative of semisynthetic cephalosporin. Int. J. Clin. Pharmacol., Therapy, Tox. 62: Shibata, K., and M. Fujii Clinical studies of cefazolin in the surgical field. Antimicrob. Ag. Chemother. 1970, p Steers, E., E. L. Foltz, B. S. Graves, and J. Riden An inocula replicating apparatus for routine testing of bacterial susceptibility to antibiotics. Antibiot. Chemother. 9: Ulmura, R Basic and clinical studies of cefazolin in surgical infections. Kagaku Ryoho 18: Wick, W. E., and D. A. Preston Biological properties of three 3-heterocyclic-thiomethyl cephalosporin antibiotics. Antimicrob. Ag. Chemother. 1:
Reassessment of the "Class" Concept of Disk Susceptibility Testing
Reassessment of the "Class" Concept of Disk Susceptibility Testing Disks versus Minimal Inhibitory Concentrations with Eleven Cephalosporins ARTHUR L. BARRY, PH.D., CLYDE THORNSBERRY, PH.D., RONALD N.
More informationIn Vitro Activity of Netilmicin, Gentamicin, and Amikacin
ANTIMICROBIAL AGzNTS AND CHEMOTHERAPY, Jan. 1977, p. 126-131 Copyright X 1977 American Society for Microbiology Vol. 11, No. 1 Printed in U.S.A. In Vitro Activity of Netilmicin, Gentamicin, and Amikacin
More informationQuality Control Testing with the Disk Antibiotic Susceptibility Test of Bauer-Kirby-Sherris-Turck
Quality Control Testing with the Disk Antibiotic Susceptibility Test of Bauer-Kirby-Sherris-Turck DONNA J. BLAZEVIC, M.P.H., MARILYN H. KOEPCKE, B.S., A JOHN M. MATSEN, M.D. Departments of Laboratory Medicine
More informationavailable. and P. aeruginosa resistant to gentamicin by standardized disk testing (1) in the Microbiology Laboratory
ANTimICROBIAL AGENTh AND CHEMOTHERAPY, OCt. 1976, p. 677-681 Copyright 1976 American Society for Microbiology Vol. 10, No. 4 Printed in U.S.A. In Vitro Susceptibility of Gentamicin-Resistant Enterobacteriaceae
More informationGeNei TM. Antibiotic Sensitivity. Teaching Kit Manual KT Revision No.: Bangalore Genei, 2007 Bangalore Genei, 2007
GeNei Bacterial Antibiotic Sensitivity Teaching Kit Manual Cat No. New Cat No. KT68 106333 Revision No.: 00180705 CONTENTS Page No. Objective 3 Principle 3 Kit Description 4 Materials Provided 5 Procedure
More informationEvaluation of a computerized antimicrobial susceptibility system with bacteria isolated from animals
J Vet Diagn Invest :164 168 (1998) Evaluation of a computerized antimicrobial susceptibility system with bacteria isolated from animals Susannah K. Hubert, Phouc Dinh Nguyen, Robert D. Walker Abstract.
More informationLab Exercise: Antibiotics- Evaluation using Kirby Bauer method.
Lab Exercise: Antibiotics- Evaluation using Kirby Bauer method. OBJECTIVES 1. Compare the antimicrobial capabilities of different antibiotics. 2. Compare effectiveness of with different types of bacteria.
More informationComparative Activity of Netilmicin, Gentamicin, Amikacin, and Tobramycin Against Pseudomonas aeruginosa and Enterobacteriaceae
ANTIMICROBIAL AGzNTS AND CHEMOTHERAPY, Oct. 1976, P. 592-597 Copyright 1976 American Society for Microbiology Vol. 1, No. 4 Printed in U.S.A. Comparative Activity of Netilmicin, Gentamicin, Amikacin, and
More informationNew Method for Antibiotic Susceptibility Testing
ANTIMIROBIAL AGENTS AND HEMOTHERAPY, Aug. 1972, p. 51-56 opyright 1972 American Society for Microbiology Vol. 2, No. 2 Printed in U.S.A. New Method for Antibiotic Susceptibility Testing G. N. ROLINSON
More informationUniversity, New York, New York Received for publication 7 May was measured by the broth dilution method as previously
ANTmIcaoBIAL AGuNTS AND CHUMTrHURAPY, Sept. 1976, p. 526-534 Copyright C 1976 American Society for Microbiology Vol. 10, No. 3 Printed in U.S.A. In Vitro Study of Netilmicin Compared with Other Aminoglycosides
More informationEvaluation of the BIOGRAM Antimicrobial Susceptibility Test System
JOURNAL OF CLINICAL MICROBIOLOGY, Nov. 1985, p. 793-798 0095-1137/85/110793-06$02.00/0 Copyright 1985, American Society for Microbiology Vol. 22, No. 5 Evaluation of the BIOGRAM Antimicrobial Susceptibility
More informationPharmacological Evaluation of Amikacin in Neonates
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, JUlY 1975, p. 86-90 Copyright 0 1975 American Society for Microbiology Vol. 8, No. 1 Printed in U.SA. Pharmacological Evaluation of Amikacin in Neonates JORGE B.
More information2 0 hr. 2 hr. 4 hr. 8 hr. 10 hr. 12 hr.14 hr. 16 hr. 18 hr. 20 hr. 22 hr. 24 hr. (time)
Key words I μ μ μ μ μ μ μ μ μ μ μ μ μ μ II Fig. 1. Microdilution plate. The dilution step of the antimicrobial agent is prepared in the -well microplate. Serial twofold dilution were prepared according
More informationIn Vitro Antimicrobial Activity of CP-99,219, a Novel Azabicyclo-Naphthyridone
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Feb. 993, p. 39-353 0066-0/93/0039-05$0.00/0 Copyright 993, American Society for Microbiology Vol. 37, No. In Vitro Antimicrobial Activity of, a Novel Azabicyclo-Naphthyridone
More informationPDF hosted at the Radboud Repository of the Radboud University Nijmegen
PDF hosted at the Radboud Repository of the Radboud University Nijmegen The following full text is a publisher's version. For additional information about this publication click this link. http://hdl.handle.net/2066/26062
More informationDetermination of antibiotic sensitivities by the
Journal of Clinical Pathology, 1978, 31, 531-535 Determination of antibiotic sensitivities by the Sensititre system IAN PHILLIPS, CHRISTINE WARREN, AND PAMELA M. WATERWORTH From the Department of Microbiology,
More informationSusceptibility Tests for Methicillin-Resistant (Heteroresistant) Staphylococci
JOURNAL OF CLNCAL MCROBOLOGY, Apr. 1984, p. 482-488 95-1137/84/4482-7$2./ Copyright C) 1984, American Society for Microbiology Vol. 19, No. 4 New Recommendations for Disk Diffusion Antimicrobial Susceptibility
More informationEXTENDED-SPECTRUM BETA-LACTAMASE (ESBL) TESTING
EXTENDED-SPECTRUM BETA-LACTAMASE (ESBL) TESTING CHN61: EXTENDED-SPECTRUM BETA-LACTAMASE (ESBL) TESTING 1.1 Introduction A common mechanism of bacterial resistance to beta-lactam antibiotics is the production
More informationComparison of tablets and paper discs for antibiotic sensitivity testing
J. clin. Path., 1975, 28, 983-988 Comparison of tablets and paper discs for antibiotic sensitivity testing D. F. J. BROWN' AND D. KOTHARI From the Division of Hospital Infection, Clinical Research Centre,
More informationSusceptibility of Staphylococcus aureus to
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Sept. 1973, p. 263-269 Copyright 0 1973 American Society for Microbiology Vol. 4, No. 3 Printed in U.S.A. Effect of Temperature on the In Vitro Susceptibility of
More informationStaphylococcus aureus with the Disc
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, May 1972, p. 422-426 Vol. 1, No. 5 Copyright 1972 American Society for Microbiology Printed in U.S.A. Identification of Cephalosporin-Resistant Staphylococcus aureus
More informationActivity of Three Aminoglycosides and Two Penicillins Against
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Feb. 1975, P. 172-178 Copyright @ 1975 American Society for Microbiology Vol. 7, No. 2 Printed in U.S.A. Activity of Three Aminoglycosides and Two Penicillins Against
More informationImproved Susceptibility Disk Assay Method Employing an
ANTIMICROIAL AGENTS AND CHEMOTHERAPY, Nov. 1978, P. 761-764 66-484/78/14-761$2./ pyright 1978 American Society for Microbiology Vol. 14, No. 5 Printed in U.S.A. Improved Susceptibility Disk Assay Method
More information6.0 ANTIBACTERIAL ACTIVITY OF CAROTENOID FROM HALOMONAS SPECIES AGAINST CHOSEN HUMAN BACTERIAL PATHOGENS
6.0 ANTIBACTERIAL ACTIVITY OF CAROTENOID FROM HALOMONAS SPECIES AGAINST CHOSEN HUMAN BACTERIAL PATHOGENS 6.1 INTRODUCTION Microorganisms that cause infectious disease are called pathogenic microbes. Although
More informationGuidelines for Laboratory Verification of Performance of the FilmArray BCID System
Guidelines for Laboratory Verification of Performance of the FilmArray BCID System Purpose The Clinical Laboratory Improvement Amendments (CLIA), passed in 1988, establishes quality standards for all laboratory
More informationTOLYPOMYCIN, A NEW ANTIBIOTIC. V IN VITRO AND IN VIVO ANTIMICROBIAL ACTIVITY. Masahiro Kondo, Tokiko Oishi and Kanji Tsuchiya
16 THE JOURNAL OF ANTIBIOTICS JAN. 1972 TOLYPOMYCIN, A NEW ANTIBIOTIC. V IN VITRO AND IN VIVO ANTIMICROBIAL ACTIVITY Masahiro Kondo, Tokiko Oishi and Kanji Tsuchiya Biological Research Laboratories, Research
More informationDiscrepancy Between Carbenicillin and Ampicillin Activities Against Enterococci and Listeria
ANTMCROBAL AGENTS AND CHEMOTHEAPY, Mar. 193, p. 3339 Copyright 193 American Society for Microbiology Vol. 3, No. 3 Printed in U.S.A. Discrepancy Between Carbenicillin and Ampicillin Activities Against
More informationThe Basics: Using CLSI Antimicrobial Susceptibility Testing Standards
The Basics: Using CLSI Antimicrobial Susceptibility Testing Standards Janet A. Hindler, MCLS, MT(ASCP) UCLA Health System Los Angeles, California, USA jhindler@ucla.edu 1 Learning Objectives Describe information
More informationVersion 1.01 (01/10/2016)
CHN58: ANTIMICROBIAL SUSCEPTIBILITY TESTING (CLSI) 1.0 PURPOSE / INTRODUCTION: 1.1 Introduction Antimicrobial susceptibility tests are performed in order to determine whether a pathogen is likely to be
More informationInternational Journal of Advances in Pharmacy and Biotechnology Vol.3, Issue-2, 2017, 1-7 Research Article Open Access.
I J A P B International Journal of Advances in Pharmacy and Biotechnology Vol.3, Issue-2, 2017, 1-7 Research Article Open Access. ISSN: 2454-8375 COMPARISON OF ANTIMICROBIAL ACTIVITY AND MIC OF BRANDED
More informationof Staphylococcus aureus
APPLIED MICROBTOLOGY, Dec. 97, p. -7 Copyright ( 97 American Society for Microbiology Vol., No. 6. Printed in U.S.A. Bacteriophage Types and Antibiotic Susceptibility of Staphylococcus aureus J. KLASTERSKY,
More informationJanuary 2014 Vol. 34 No. 1
January 2014 Vol. 34 No. 1. and Minimal Inhibitory Concentration (MIC) Interpretive Standards for Testing Conditions Medium: diffusion: Mueller-Hinton agar (MHA) roth dilution: cation-adjusted Mueller-Hinton
More informationQ1. (a) Clostridium difficile is a bacterium that is present in the gut of up to 3% of healthy adults and 66% of healthy infants.
Q1. (a) Clostridium difficile is a bacterium that is present in the gut of up to 3% of healthy adults and 66% of healthy infants. C. difficile rarely causes problems, either in healthy adults or in infants.
More informationDrug resistance in relation to use of silver sulphadiazine cream in a burns unit
J. clin. Path., 1977, 30, 160-164 Drug resistance in relation to use of silver sulphadiazine cream in a burns unit KIM BRIDGES AND E. J. L. LOWBURY From the MRC Industrial Injuries and Burns Unit, Birmingham
More informationANTIMICROBIAL TESTING. with ALKA VITA (ALKAHYDROXY ) ESCHERICHIA COLI STAPHYLOCOCCUS AUREUS (MRSA) PSEUDOMONA AERUGINOSA ENTEROBACTER CLOACAE
ANTIMICROBIAL TESTING with ALKA VITA (ALKAHYDROXY ) on ESCHERICHIA COLI STAPHYLOCOCCUS AUREUS (MRSA) PSEUDOMONA AERUGINOSA ENTEROBACTER CLOACAE FINAL RESULTS OF ANTIBACTERIAL TESTS IN VITRO WITH THE PRODUCT
More informationPerformance Information. Vet use only
Performance Information Vet use only Performance of plates read manually was measured in three sites. Each centre tested Enterobacteriaceae, streptococci, staphylococci and pseudomonas-like organisms.
More informationSusceptibility Testing
APPLIED MICROBIOLOGY, Nov. 1969, p. 766-770 Copyright 1969 American Society for Microbiology Vol. 18, No. 5 Printed in U.S.A. Effect of Mixed Cultures on Antibiotic Susceptibility Testing AZRA SHAHIDI
More informationEffects of Minocycline and Other Antibiotics on Fusobacterium necrophorum Infections in Mice
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Apr. 1975, p. 421-425 Copyright 0 1975 American Society for Microbiology Vol. 7, No. 4 Printed in U.S.A. Effects of Minocycline and Other s on Fusobacterium necrophorum
More informationChapter 2. Disk diffusion method
Chapter 2. Disk diffusion method Tendencia, Eleonor A. Date published: 2004 To cite this document : Tendencia, E. A. (2004). Chapter 2. Disk diffusion method. In Laboratory manual of standardized methods
More informationTel: Fax:
CONCISE COMMUNICATION Bactericidal activity and synergy studies of BAL,a novel pyrrolidinone--ylidenemethyl cephem,tested against streptococci, enterococci and methicillin-resistant staphylococci L. M.
More informationKlebsiella, Enterobacter, and Serratia:
APPLIED MICROBIOLOGY, Aug. 1969, p. 198-203 Copyright 1969 American Society for Microbiology Klebsiella, Enterobacter, and Serratia: Vol. 18, No. 2 Printed in U.S.A. Biochemical Differentiation and Susceptibility
More informationMICHAEL J. RYBAK,* ELLIE HERSHBERGER, TABITHA MOLDOVAN, AND RICHARD G. GRUCZ
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Apr. 2000, p. 1062 1066 Vol. 44, No. 4 0066-4804/00/$04.00 0 Copyright 2000, American Society for Microbiology. All Rights Reserved. In Vitro Activities of Daptomycin,
More informationSynergism of penicillin or ampicillin combined with sissomicin or netilmicin against enterococci
Journal of Antimicrobial Chemotherapy (78) 4, 53-543 Synergism of penicillin or ampicillin combined with sissomicin or netilmicin against enterococci Chatrchal Watanakunakoni and Cheryl Glotzbecker Infectious
More information17June2017. Parampal Deol, Ph.D, MBA Senior Director, R&D Microbiology North America
RAPID DETECTION OF BACTERIAL CONTAMINANTS IN PLATELET COMPONENTS: COMPARISON OF TIME TO DETECTION BETWEEN THE BACT/ALERT 3D AND THE BACT/ALERT VIRTUO SYSTEMS. 17June2017 Parampal Deol, Ph.D, MBA Senior
More informationAminoglycoside-resistant enterococci
Aminoglycoside-resistant enterococci M. J. BASKER, B. SLOCOMBE, AND R. SUTHERLAND From Beecham Pharmaceuticals Research Division, Brockham Park, Betchworth, Surrey J. clin. Path., 1977, 30, 375-380 SUMMARY
More informationCOMPARATIVE STUDY OF ANTIBIOTICS FOR THEIR ANTIMICROBIAL SUSCEPTIBILITY IN CLINICAL ISOLATES
IJPSR (2015), Vol. 6, Issue 2 (Research Article) Received on 03 July, 2014; received in revised form, 03 September, 2014; accepted, 19 January, 2015; published 01 February, 2015 COMPARATIVE STUDY OF ANTIBIOTICS
More informationa. 379 laboratories provided quantitative results, e.g (DD method) to 35.4% (MIC method) of all participants; see Table 2.
AND QUANTITATIVE PRECISION (SAMPLE UR-01, 2017) Background and Plan of Analysis Sample UR-01 (2017) was sent to API participants as a simulated urine culture for recognition of a significant pathogen colony
More informationEffeet on Bacterial Growth
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Nov. 17, p. 36-366 Copyright ( 17 American Society for Microbiology Vol., No. 5 Printed in U.S.A. Automatic Radiometric Measurement of Antibiotic Effeet on Bacterial
More informationDefining Extended Spectrum b-lactamases: Implications of Minimum Inhibitory Concentration- Based Screening Versus Clavulanate Confirmation Testing
Infect Dis Ther (2015) 4:513 518 DOI 10.1007/s40121-015-0094-6 BRIEF REPORT Defining Extended Spectrum b-lactamases: Implications of Minimum Inhibitory Concentration- Based Screening Versus Clavulanate
More informationConcise Antibiogram Toolkit Background
Background This toolkit is designed to guide nursing homes in creating their own antibiograms, an important tool for guiding empiric antimicrobial therapy. Information about antibiograms and instructions
More information4 th and 5 th generation cephalosporins. Naderi HR Associate professor of Infectious Diseases
4 th and 5 th generation cephalosporins Naderi HR Associate professor of Infectious Diseases Classification Forth generation: Cefclidine, cefepime (Maxipime),cefluprenam, cefoselis,cefozopran, cefpirome
More informationagainst Clinical Isolates of Gram-Positive Bacteria
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Feb. 993, p. 366-370 Vol. 37, No. 0066-0/93/00366-05$0.00/0 Copyright 993, American Society for Microbiology In Vitro Activity of CP-99,9, a New Fluoroquinolone,
More information2017 Antibiogram. Central Zone. Alberta Health Services. including. Red Deer Regional Hospital. St. Mary s Hospital, Camrose
2017 Antibiogram Central Zone Alberta Health Services including Red Deer Regional Hospital St. Mary s Hospital, Camrose Introduction This antibiogram is a cumulative report of the antimicrobial susceptibility
More information2016 Antibiotic Susceptibility Report
Fairview Northland Medical Center and Elk River, Milaca, Princeton and Zimmerman Clinics 2016 Antibiotic Susceptibility Report GRAM-NEGATIVE ORGANISMS 2016 Gram-Negative Non-Urine The number of isolates
More informationComparative In Vitro Activity of Prulifloxacin against Bacteria Isolated from Hospitalized Patients at Siriraj Hospital
Original Article Vol. 27 No. 2 In vitro activity of prulifloxacin against clinical bacterial isolates:- Thamlikitkul V & Tiengrim S. 61 Comparative In Vitro Activity of n against Bacteria Isolated from
More informationDevelopment of Resistant Bacteria Isolated from Dogs with Otitis Externa or Urinary Tract Infections after Exposure to Enrofloxacin In Vitro
A. M. Brothers, P. S. Gibbs, and R. E. Wooley Development of Resistant Bacteria Isolated from Dogs with Otitis Externa or Urinary Tract Infections after Exposure to Enrofloxacin In Vitro Amy M. Brothers,
More informationMechanism of Chloramphenicol-Cephaloridine Synergism on Enterobacteriaceae
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, June 1975, p. 845-849 Copyright 0 1975 American Society for Microbiology Vol. 7, No. 6 Printed in U.S.A. Mechanism of -Cephaloridine Synergism on Enterobacteriaceae
More informationEvaluation of the AutoMicrobic System for Susceptibility Testing of Aminoglycosides and Gram-Negative Bacilli
JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 1987, p. 546-550 0095-1137/87/030546-05$02.00/0 Copyright C 1987, American Society for Microbiology Vol. 25, No. 3 Evaluation of the AutoMicrobic System for Susceptibility
More informationSENSITIVE AND -RESISTANT TUBERCLE BACILLI IN LIQUID MEDIUM SENSITIVITY TESTS
Thorax (195), 5, 162. THE BEHAVIOUR OF MIXTURES OF STREPTOMYCIN- SENSITIVE AND -RESISTANT TUBERCLE BACILLI IN LIQUID MEDIUM SENSITIVITY TESTS BY D. A. MITCHISON* From the Department of Bacteriology, Postgraduate
More informationSynergy Between Cephalosporin and Aminoglycoside
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, June 1974, P. 571--577 Copyright 0 1974 American Society for Microbiology Vol. 5, No. 6 Printed in U.S.A. Synergy Between Cephalosporin and Aminoglycoside Antibiotics
More informationAntimicrobial Susceptibility Testing: The Basics
Antimicrobial Susceptibility Testing: The Basics Susan E. Sharp, Ph.D., DABMM, FAAM Director, Airport Way Regional Laboratory Director, Regional Microbiology and Molecular Infectious Diseases Laboratories
More informationAntimicrobial Drug on Drug Resistance in the Lactose-Fermenting Enteric Flora
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, May 1975, p. 661-665 Copyright O 1975 American Society for Microbiology Vol. 7, No. 5 Printed in U.S.A. Animal Model for Determining the No-Effect Level of an Antimicrobial
More informationComparison of antibiotic susceptibility results obtained with Adatab* and disc methods
J Clin Pathol 1984;37:159-165 Comparison of antibiotic susceptibility results obtained with Adatab* and disc methods JJS SNELL, MVS DANVERS, PS GARDNER From the Division of Microbiological Reagents and
More informationOriginal Article. Ratri Hortiwakul, M.Sc.*, Pantip Chayakul, M.D.*, Natnicha Ingviya, B.Sc.**
Original Article In Vitro Activity of Cefminox and Other β-lactam Antibiotics Against Clinical Isolates of Extended- Spectrum-β-lactamase-Producing Klebsiella pneumoniae and Escherichia coli Ratri Hortiwakul,
More informationBackground and Plan of Analysis
ENTEROCOCCI Background and Plan of Analysis UR-11 (2017) was sent to API participants as a simulated urine culture for recognition of a significant pathogen colony count, to perform the identification
More informationAPPENDIX III - DOUBLE DISK TEST FOR ESBL
Policy # MI\ANTI\04\03\v03 Page 1 of 5 Section: Antimicrobial Susceptibility Testing Manual Subject Title: Appendix III - Double Disk Test for ESBL Issued by: LABORATORY MANAGER Original Date: January
More information2012 ANTIBIOGRAM. Central Zone Former DTHR Sites. Department of Pathology and Laboratory Medicine
2012 ANTIBIOGRAM Central Zone Former DTHR Sites Department of Pathology and Laboratory Medicine Medically Relevant Pathogens Based on Gram Morphology Gram-negative Bacilli Lactose Fermenters Non-lactose
More informationESBL Producers An Increasing Problem: An Overview Of An Underrated Threat
ESBL Producers An Increasing Problem: An Overview Of An Underrated Threat Hicham Ezzat Professor of Microbiology and Immunology Cairo University Introduction 1 Since the 1980s there have been dramatic
More informationJAC Bactericidal index: a new way to assess quinolone bactericidal activity in vitro
Journal of Antimicrobial Chemotherapy (1997) 39, 713 717 JAC Bactericidal index: a new way to assess quinolone bactericidal activity in vitro Ian Morrissey* Department of Biosciences, Division of Biochemistry
More informationAcquired and Native Resistance of Staphylococcus
APPLED MCROBOLOGY, JUlY 1970, p. 1-5 Copyright 1970 American Society for Microbiology Vol. 20, No.1 Printed in U.S.A. Acquired and Native Resistance of Staphylococcus aureus to Cephalexin and Other f3-lactam
More informationThe Disinfecting Effect of Electrolyzed Water Produced by GEN-X-3. Laboratory of Diagnostic Medicine, College of Medicine, Soonchunhyang University
The Disinfecting Effect of Electrolyzed Water Produced by GEN-X-3 Laboratory of Diagnostic Medicine, College of Medicine, Soonchunhyang University Tae-yoon Choi ABSTRACT BACKGROUND: The use of disinfectants
More informationAntimicrobial Stewardship Strategy: Antibiograms
Antimicrobial Stewardship Strategy: Antibiograms A summary of the cumulative susceptibility of bacterial isolates to formulary antibiotics in a given institution or region. Its main functions are to guide
More informationAntibiotic Sensitivity Pattern in Bacterial Endocarditis
Abstract Antibiotic Sensitivity Pattern in Bacterial Endocarditis Pages with reference to book, From 129 To 132 Pirzada, M.U. Siddiqui ( Dept. of Microbiology, University of Karachi. ) Seventyone blood
More informationUnderstanding the Hospital Antibiogram
Understanding the Hospital Antibiogram Sharon Erdman, PharmD Clinical Professor Purdue University College of Pharmacy Infectious Diseases Clinical Pharmacist Eskenazi Health 5 Understanding the Hospital
More information2015 Antibiotic Susceptibility Report
Citrobacter freundii Enterobacter aerogenes Enterobacter cloacae Escherichia coli Haemophilus influenzenza Klebsiella oxytoca Klebsiella pneumoniae Proteus mirabilis Pseudomonas aeruginosa Serratia marcescens
More informationReceived 10 November 2006/Returned for modification 9 January 2007/Accepted 17 July 2007
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Oct. 2007, p. 3726 3730 Vol. 51, No. 10 0066-4804/07/$08.00 0 doi:10.1128/aac.01406-06 Copyright 2007, American Society for Microbiology. All Rights Reserved. Comparative
More informationMercy Medical Center Des Moines, Iowa Department of Pathology. Microbiology Department Antibiotic Susceptibility January December 2016
Mercy Medical Center Des Moines, Iowa Department of Pathology Microbiology Department Antibiotic Susceptibility January December 2016 These statistics are intended solely as a GUIDE to choosing appropriate
More informationOriginal Article. Hossein Khalili a*, Rasool Soltani b, Sorrosh Negahban c, Alireza Abdollahi d and Keirollah Gholami e.
Iranian Journal of Pharmaceutical Research (22), (2): 559-563 Received: January 2 Accepted: June 2 Copyright 22 by School of Pharmacy Shaheed Beheshti University of Medical Sciences and Health Services
More informationVOL. XXIII NO. II THE JOURNAL OF ANTIBIOTICS 559. ANTIBIOTIC 6640.* Ill
VOL. XXIII NO. II THE JOURNAL OF ANTIBIOTICS 559 ANTIBIOTIC 6640.* Ill BIOLOGICAL STUDIES WITH ANTIBIOTIC 6640, A NEW BROAD-SPECTRUM AMINOGLYCOSIDE ANTIBIOTIC J. Allan Waitz, Eugene L. Moss, Jr., Edwin
More informationTEST REPORT. Client: M/s Ion Silver AB. Loddekopinge. Sverige / SWEDEN. Chandran. min and 30 min. 2. E. coli. 1. S. aureus
TEST REPORT TEST TYPE: Liquid Suspension Time Kill Study -Quantitative Test Based On ASTM 2315 TEST METHOD of Colloidal Silver Product at Contact time points: 30 sec, 1 min, 2 min, 5 min, 10 min, 15 min
More informationTHE STABILITY OF E1VROFLOXA CIN University Undergraduate Research Fellow. A Senior Thesis. Texas ASM University.
THE STABILITY OF E1VROFLOXA CIN A Senior Thesis By Meagan A. Dodge 1997-98 University Undergraduate Research Fellow Texas ASM University Group: Biology THE STABILITY OF ENROFLOXACIN MEAGANA, DODGE Submitted
More informationDepartment of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
Volume 8 Number 5 (October 206) 307-3 ORIGINAL ARTICLE A comparison of antibiotic disks from different sources on Quicolor and Mueller-Hinton agar media in evaluation of antibacterial susceptibility testing
More informationAn evaluation of the susceptibility patterns of Gram-negative organisms isolated in cancer centres with aminoglycoside usage
Journal of Antimicrobial Chemotherapy (1991) 27, Suppl. C, 1-7 An evaluation of the susceptibility patterns of Gram-negative organisms isolated in cancer centres with aminoglycoside usage J. J. Muscato",
More informationHelp with moving disc diffusion methods from BSAC to EUCAST. Media BSAC EUCAST
Help with moving disc diffusion methods from BSAC to EUCAST This document sets out the main differences between the BSAC and EUCAST disc diffusion methods with specific emphasis on preparation prior to
More informationDefining Resistance and Susceptibility: What S, I, and R Mean to You
Defining Resistance and Susceptibility: What S, I, and R Mean to You Michael D. Apley, DVM, PhD, DACVCP Department of Clinical Sciences College of Veterinary Medicine Kansas State University Susceptible
More informationESCMID Online Lecture Library. by author
Quality Assurance of antimicrobial susceptibility testing Derek Brown EUCAST Scientific Secretary ESCMID Postgraduate Education Course, Linz, 17 September 2014 Quality Assurance The total process by which
More informationFg/ml into the gentamicin and tobramycin panels, and 12 and 24 pig/ml into the amikacin. panels. Minimal inhibitory concentration (MIC)
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Nov. 1983, p. 764-770 00664804/83/110764-07$02.00/0 Copyright C 1983, American Society for Microbiology Vol. 24, No. 5 Error Rates Associated With the Use of Recently
More informationSAMPLE. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals
VET01 5th Edition Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals This standard covers the current recommended methods for disk diffusion
More informationEXPERIMENT. Antibiotic Sensitivity-Kirby Bauer Diffusion Test
EXPERIMENT Antibiotic Sensitivity-Kirby Bauer Diffusion Test Author Name Version 42-0238-00-02 Review the safety materials and wear goggles when working with chemicals. Read the entire exercise before
More informationPrinciples and Practice of Antimicrobial Susceptibility Testing. Microbiology Technical Workshop 25 th September 2013
Principles and Practice of Antimicrobial Susceptibility Testing Microbiology Technical Workshop 25 th September 2013 Scope History Why Perform Antimicrobial Susceptibility Testing? How to Perform an Antimicrobial
More informationBIOLACTAM. Product Description. An innovative in vitro diagnostic for the rapid quantitative determination of ß-lactamase activity
BIOLACTAM www.biolactam.eu An innovative in vitro diagnostic for the rapid quantitative determination of ß-lactamase activity 1.5-3h 20 Copyright 2014 VL-Diagnostics GmbH. All rights reserved. Product
More informationRELIABLE AND REALISTIC APPROACH TO SENSITIVITY TESTING
RELIABLE AND REALISTIC APPROACH TO SENSITIVITY TESTING Pages with reference to book, From 94 To 97 S. Hafiz, N. Lyall, S. Punjwani, Shahida Q. Zaidi ( Department of Microbiology, The Aga Khan University
More informationcoverage. The purpose of this study was to compare the susceptibility of 297 strains of anaerobic and a new broad-spectrum semisynthetic
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Nov. 1978, p. 643-649 0066-4804/78/0014-0643$02.00/0 Copyright 1978 American Society for Microbiology Vol. 14, No. 5 Printed in U.S.A. Susceptibility of the Anaerobic
More informationVisit ABLE on the Web at:
This article reprinted from: Lessem, P. B. 2008. The antibiotic resistance phenomenon: Use of minimal inhibitory concentration (MIC) determination for inquiry based experimentation. Pages 357-362, in Tested
More informationReceived 5 February 2004/Returned for modification 16 March 2004/Accepted 7 April 2004
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Aug. 2004, p. 3112 3118 Vol. 48, No. 8 0066-4804/04/$08.00 0 DOI: 10.1128/AAC.48.8.3112 3118.2004 Copyright 2004, American Society for Microbiology. All Rights Reserved.
More informationVLLM0421c Medical Microbiology I, practical sessions. Protocol to topic J05
Topic J05: Determination of susceptibility of bacteria to antimicrobial drugs, assessments of resistance factors For study: textbooks, www, keywords e. g. Diffusion disc test ; E-test ; dilution micromethod
More informationAntibacterial susceptibility testing
Antibiotics: Antil susceptibility testing are natural chemical substances produced by certain groups of microorganisms (fungi, ) that inhibit the growth of or kill the other that cause infection. Several
More informationAntibacterial Agents & Conditions. Stijn van der Veen
Antibacterial Agents & Conditions Stijn van der Veen Antibacterial agents & conditions Antibacterial agents Disinfectants: Non-selective antimicrobial substances that kill a wide range of bacteria. Only
More informationCUMULATIVE ANTIBIOGRAM
BC Children s Hospital and BC Women s Hospital & Health Centre CUMULATIVE ANTIBIOGRAM 2017 Division of Medical Microbiology Department of Pathology and Laboratory Medicine Page 1 of 5 GRAM-POSITIVE BACTERIA
More informationPractical approach to Antimicrobial susceptibility testing (AST) and quality control
Practical approach to Antimicrobial susceptibility testing (AST) and quality control A/Professor John Ferguson, Microbiologist & Infectious Diseases Physician, Pathology North, University of Newcastle,
More information