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1 // OUTLIE OF PRESETATIO RECET STUDIES OF PARASITIC IFECTIO OF RUMIATS I BOGOR AGRICULTURAL UIVERSITY ITRODUCTIO STUDI O THE PREVALECE AD RISK FACTORS OF EDOPARASITE IFECTIOS I SMALL HOLDER BEEF CATTLE FARMS OF WEST JAVA PROVICE DEVELOPMET OF ELISA FOR DETECTIO OF LIVER FLUKE (F. GIGATICA) COPROATIGE I Fadjar Satrija ational Zoonosis Center Department of Animal Diseases and Veterinary Public Health, Faculty of Veterinary Medicine Bogor Agricultural University ITRODUCTIO Ruminants as an important source of animal origin protein in Indonesia Government cattle-buffalo meat self sufficient program 4 Socio-cultural importance of ruminants for Indonesian people Tropical country : favorable environment for pathogens including parasite RUMIATS ACKOWLEDGMETS STUDI O THE PREVALECE AD RISK FACTORS OF EDOPARASITE IFECTIOS I SMALL HOLDER BEEF CATTLE FARMS OF WEST JAVA PROVICE Y. Ridwan Ridwan,, F. Satrija EB Retnani Retnani,, R.Tiuria R. Tiuria,, U. Cahyaningsih Cahyaningsih,, A. Budiman Division of Parasitology and Medical Entomology, Department of Animal Diseases an Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University

2 // Background Objectives Parasite Meat self sufficient To identify types and prevalence of endo parasites that infect beef cattle on farms in rural area of West Java Province To identify risk factors endo parasite infection in beef cattle in farms Basic data of parasite epidemiology Disease control, incl parasites Study Design Cross sectional Metode multi stage sampling (Win episcope.) with basic assumptions: Sensitivity 95 Prevalence Level of confidence 95 Fecal and blood smear samples Questionnaire for farmer (farming management) Study Design Period Study area Kabupaten Ciamis August September Kabupaten Tasikmalaya Kabupaten Sumedang Kabupaten Subang Kabupaten Cianjur Kabupaten Sukabumi

3 // umber of sample and farmer o Sampling area (Regency) Cattle population umber of sample umber of farmer Cianjur Ciamis Subang Sumedang Sukabumi Tasikmalaya Total sample Prevalence of GI ematode Infection in Cattle Cattle P Prevalence of parasite infections Prevalence of Toxocarosis vitulorum in Cattle Cattle Sapi umur (> th) Sapi umur (6- th) Sapi umur (>- th) Sapi umur (-6 bln) 7 4

4 // Prevalence of Cestodosis dan Trematodosis in Cattle Prevalence of blood parasite infections in cattle Anaplasma Monezia Fasciola Prevalence of coccidiosis in cattle Cianjur Sukabumi Sumedang Tasikmalaya 6.9 Provinsi Prevalence of cryptosporidiosis 4.9 Coccidiosis Subang Ciamis 8.6 Trypanosoma Theileria Babesia Parampistomum

5 // Cattle rearing system Kategori M Kept in barn (Zero grazing) Grazing during day time and barned in the night Method of rearing cattle Grazing all the time Every day Farm Management A number of time within a week Grazing Frequency A number of time within a month Place of Grazing Cattle barn Barn floor Concrete 76. Soil Wood 8.7 ot regular. Regular Every day Barn cleaning Frequency of One a week barn cleaning Couple days in a week o regular schedule Others Paddy field (after harvest) Estate area (coconut, rubber estate) Forest Common grazing area Others Abortion Abortion cases with the last one year 98 o 6 Yes Occurrence of abortion case in the herd Always Seldom 4 Rare ot known

6 // Calves Health Calves Mortality Occurrence of o sickness Yes Symptoms of sickness 6 9 Diarrhea Infeksi tali pusar Worm expulsion from rectum Constipation afsu makan turun Emaciation Dull hair Others Anthelmintics Anthelmintic usage Type of anthelmintic Source of anthelmintic Frequency of anthelmintic treatment o Yes Standarized herbal medicine (Jamu) Herbal medicine Commercial drug Others Livestock Service Vet drug TS Purchase of its own x / year x / year x / year 4 x / year Clinical cases Post partus Farmer Occurrence of calves mortality o Yes Clinical signs prior to mortality Diarrhea loss of appetite Emaciated Dull hair Risk factors relating to blood parasite infections Cattle rearing method (Grazing cattle) Frequency of grazing (Every day, weekly) Place of grazing (estate area, post harvest paddy field) Presence of flies and tick (often found) Control of flies and tick (manual removal, no treatment) 6

7 // Risk factors relating to Trematodes infections Rearing method (Grazing cattle) Frequency of grazing (all time grazing) Place for grazing (post harvest paddy field, estate area) Part of straw for feeding (all part, ¾ lower) Grass for feeding (fresh grass) Source of grass (common grazing land) Source of water (river) Frequency of anthelmintic treatment (only when animal sick) Risk factors relating to Coccidia infections Rearing method (Grazing all time) Place of grazing (common grazing land) Barn floor (soil, wood) Frequency of barn cleaning (x/week) Risk factors relating to Gastrointestinal ematode infections Rearing method (Grazing cattle) Frequency of grazing (Every day) Place of grazing (common grazing land) Part of straw for feeding (¾ lower) Source of grass (common grazing land) Frequency of anthelmintic treatment (only when animal sick) DEVELOPMET OF ELISA FOR DETECTIO OF LIVER FLUKE (F. GIGATICA) COPROATIGE I RUMIATS F. Satrija, S. Murtini, Y. Ridwan Department of Animal Diseases an Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University 7

8 // Objective Background Fasciolosis has been known as one of most economically important parasite of ruminants in Indonesia Potential zoonosis? eeds for early detection tools for effective control To develop ELISA for detection of coproantigen as a diagnostic tool for early detection of Fasciola gigantica infection Study Design Isolation and characterization of excretory/secretory (ES) protein from F. gigantica Production of polyclonal antibody (IgY and Ig G) anti-es Development of ELISA Specificity and sensitivity test of ELISA 8

9 // Profile of ES protein antigen Marker ELISA design Sandwich ELISA Capture antibody IgY anti-es Antigen: fecal suspension Detection antibody IgG anti-es Secondary antibody goat anti rabbit IgG HRP conjugated Subtrate (TMB) ES Comparison between ELISA results and worm counting (gold standard) Post mortem Positive ELISA result egative Total Positive 7 (a) (b) 7 egative (c) 9 (d) Total Sensitivity a/a+c x 87,5 Specificity d/b+d x 9

10 // Trial using field samples Result of microscopic observation Positive Result Positive of ELISA egative Total egative Total 9 (a) (b) 6 (c) 6 (d) Aknowledgments West Java Province Livestock Service Competitive Research Grant, Directorate General of Higher Education, Ministry of Education & Culture, GOI Faculty of Animal Science, IPB Thank you very much for your attention

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